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The map label for this gene is aphA [H]

Identifier: 99080671

GI number: 99080671

Start: 870579

End: 871763

Strand: Reverse

Name: aphA [H]

Synonym: TM1040_0830

Alternate gene names: 99080671

Gene position: 871763-870579 (Counterclockwise)

Preceding gene: 99080672

Following gene: 99080670

Centisome position: 27.23

GC content: 51.9

Gene sequence:

>1185_bases
ATGACAAAAAATGCACAAGCAGTTTCCAAGCCAACCTCTGCAAAACACTACCTAGAAGAAGAGCTTGATGCACTCTTCCA
ACAGGATGCGGAGGCTTGGCGTTTTGTTCAGCAAGGGTCGCTTGACGGGGTTTGGTACTGGGATCTTAAGCAGCCTGACA
AAGAATGGATGAGCCCCGAGATGTGGGCGCTATTTGGCATAGATCCCACCACAAAGCGTCACGACCCGTCAGAGTGGCAG
GACATCATTTTCAAAGAAGACCTCGAGGTTGCTCTCAAGAATTTTGAAAAGCACTGCGCAGATCCCAATCACCCCTATGA
TCAGATTGTGCGGTATCGTCACGCCGATGGATCGACGGTTTGGGTCCGGTGTCGAGGCATTGCTATCCGTGACGAAACAG
GAAAACCCATCCGTATGTTGGGCGCGCACAATGACCTGACGGCGGTCAAACTTGCTGAGGAAAGAGCAAACACTGCCTGC
AGGGAAACTGCTGCTGCCAATGAAGAACTGCGCAGCTTTGCGTATAGTATTTCCCATGATCTCAAGTCCCCCACTAATAC
AATGGACATGCTCCTGCGAGAGATCGCTATTGCGGACGATGGTGGCATGTCAGAAGAACAGCGCGAGCTGCTGCATACGG
CGCAAGAAACGGCGAATAGAATGCGCAGCCTTGTTGATGACATGCTGGACTACACGCGGTTAATTGGCGAGGAGCCTGTG
TGGGAGAGCGTTTCGCTTTACGATACCGGGACGCGTGTTCGGGATATGCTGGGATCGCTCGTGGCCGAGACAGGTGCCAA
AATTTCGATCGACGAAAACCTTCCCAATGTGTCAGGCAACCCCACCCAGTTGAGGGTATTATTACAAAACCTTATCGAGA
ACGCCATCAAGTACCGCGATCCCTCGCGTCCGCCGGTTGTGGTTGTTTCTAAACTCCACGCTGCTGACACGAATGAAACT
GGTTTTTCGGTAGCCGATAACGGCTTGGGCATAGCAGAGGGGCATCAAGACCGTGTTTTCGAGATGTTCAAACGTTTACA
CCGCGAAGATGAAGTTGCTGGGGCGGGTTTGGGTTTAACTCTTTGCCGTCGCGTGGCGCTAAACCATGGTGGGGATATTC
AAGTCTCCTCGAAGCTTGGCCAAGGAACAACTTTCACCGTATCTTTTCCGGAGCGAAACACATGA

Upstream 100 bases:

>100_bases
CCGTGTCGAGCAGCTAAATAATTTATGCATTCTTAACACGATGCAGATAGGAATTTTGTGCATGTTTCAGTGACACGGTT
ATTTGGTGGTCAGGTAAAAA

Downstream 100 bases:

>100_bases
GCACTTCATCTCCCATTGGCTCGATAATGCTCATCGACGATAACGAGATTGACCATCGCATGTGCAAACGAGTGGTCGAA
CGCTCAGGGTTGGTCGGGGA

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTKNAQAVSKPTSAKHYLEEELDALFQQDAEAWRFVQQGSLDGVWYWDLKQPDKEWMSPEMWALFGIDPTTKRHDPSEWQ
DIIFKEDLEVALKNFEKHCADPNHPYDQIVRYRHADGSTVWVRCRGIAIRDETGKPIRMLGAHNDLTAVKLAEERANTAC
RETAAANEELRSFAYSISHDLKSPTNTMDMLLREIAIADDGGMSEEQRELLHTAQETANRMRSLVDDMLDYTRLIGEEPV
WESVSLYDTGTRVRDMLGSLVAETGAKISIDENLPNVSGNPTQLRVLLQNLIENAIKYRDPSRPPVVVVSKLHAADTNET
GFSVADNGLGIAEGHQDRVFEMFKRLHREDEVAGAGLGLTLCRRVALNHGGDIQVSSKLGQGTTFTVSFPERNT

Sequences:

>Translated_394_residues
MTKNAQAVSKPTSAKHYLEEELDALFQQDAEAWRFVQQGSLDGVWYWDLKQPDKEWMSPEMWALFGIDPTTKRHDPSEWQ
DIIFKEDLEVALKNFEKHCADPNHPYDQIVRYRHADGSTVWVRCRGIAIRDETGKPIRMLGAHNDLTAVKLAEERANTAC
RETAAANEELRSFAYSISHDLKSPTNTMDMLLREIAIADDGGMSEEQRELLHTAQETANRMRSLVDDMLDYTRLIGEEPV
WESVSLYDTGTRVRDMLGSLVAETGAKISIDENLPNVSGNPTQLRVLLQNLIENAIKYRDPSRPPVVVVSKLHAADTNET
GFSVADNGLGIAEGHQDRVFEMFKRLHREDEVAGAGLGLTLCRRVALNHGGDIQVSSKLGQGTTFTVSFPERNT
>Mature_393_residues
TKNAQAVSKPTSAKHYLEEELDALFQQDAEAWRFVQQGSLDGVWYWDLKQPDKEWMSPEMWALFGIDPTTKRHDPSEWQD
IIFKEDLEVALKNFEKHCADPNHPYDQIVRYRHADGSTVWVRCRGIAIRDETGKPIRMLGAHNDLTAVKLAEERANTACR
ETAAANEELRSFAYSISHDLKSPTNTMDMLLREIAIADDGGMSEEQRELLHTAQETANRMRSLVDDMLDYTRLIGEEPVW
ESVSLYDTGTRVRDMLGSLVAETGAKISIDENLPNVSGNPTQLRVLLQNLIENAIKYRDPSRPPVVVVSKLHAADTNETG
FSVADNGLGIAEGHQDRVFEMFKRLHREDEVAGAGLGLTLCRRVALNHGGDIQVSSKLGQGTTFTVSFPERNT

Specific function: Photoreceptor which exists in two forms that are reversibly interconvertible by light:the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=297, Percent_Identity=28.2828282828283, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789149, Length=244, Percent_Identity=27.4590163934426, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788713, Length=251, Percent_Identity=25.4980079681275, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786600, Length=235, Percent_Identity=29.3617021276596, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786783, Length=229, Percent_Identity=28.82096069869, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786912, Length=346, Percent_Identity=26.0115606936416, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790436, Length=228, Percent_Identity=27.1929824561404, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI145693157, Length=259, Percent_Identity=25.0965250965251, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081816, Length=241, Percent_Identity=23.6514522821577, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=26.2008733624454, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1788549, Length=268, Percent_Identity=25, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR013654
- InterPro:   IPR016132
- InterPro:   IPR001294
- InterPro:   IPR013515
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF01590 GAF; PF02518 HATPase_c; PF00512 HisKA; PF08446 PAS_2; PF00360 Phytochrome [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44308; Mature: 44177

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNAQAVSKPTSAKHYLEEELDALFQQDAEAWRFVQQGSLDGVWYWDLKQPDKEWMSPE
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHCCCC
MWALFGIDPTTKRHDPSEWQDIIFKEDLEVALKNFEKHCADPNHPYDQIVRYRHADGSTV
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEE
WVRCRGIAIRDETGKPIRMLGAHNDLTAVKLAEERANTACRETAAANEELRSFAYSISHD
EEEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKSPTNTMDMLLREIAIADDGGMSEEQRELLHTAQETANRMRSLVDDMLDYTRLIGEEPV
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WESVSLYDTGTRVRDMLGSLVAETGAKISIDENLPNVSGNPTQLRVLLQNLIENAIKYRD
CCCEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PSRPPVVVVSKLHAADTNETGFSVADNGLGIAEGHQDRVFEMFKRLHREDEVAGAGLGLT
CCCCCEEEEEEHHCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
LCRRVALNHGGDIQVSSKLGQGTTFTVSFPERNT
HHHHHHHCCCCCEEEECCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TKNAQAVSKPTSAKHYLEEELDALFQQDAEAWRFVQQGSLDGVWYWDLKQPDKEWMSPE
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHCCCC
MWALFGIDPTTKRHDPSEWQDIIFKEDLEVALKNFEKHCADPNHPYDQIVRYRHADGSTV
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEE
WVRCRGIAIRDETGKPIRMLGAHNDLTAVKLAEERANTACRETAAANEELRSFAYSISHD
EEEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKSPTNTMDMLLREIAIADDGGMSEEQRELLHTAQETANRMRSLVDDMLDYTRLIGEEPV
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WESVSLYDTGTRVRDMLGSLVAETGAKISIDENLPNVSGNPTQLRVLLQNLIENAIKYRD
CCCEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PSRPPVVVVSKLHAADTNETGFSVADNGLGIAEGHQDRVFEMFKRLHREDEVAGAGLGLT
CCCCCEEEEEEHHCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
LCRRVALNHGGDIQVSSKLGQGTTFTVSFPERNT
HHHHHHHCCCCCEEEECCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]