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The map label for this gene is tpiA

Identifier: 99080605

GI number: 99080605

Start: 820756

End: 821496

Strand: Reverse

Name: tpiA

Synonym: TM1040_0764

Alternate gene names: 99080605

Gene position: 821496-820756 (Counterclockwise)

Preceding gene: 99080606

Following gene: 99080575

Centisome position: 25.66

GC content: 64.24

Gene sequence:

>741_bases
ATGGCGCGCAAACTCGCGGCAGGCAACTGGAAAATGAACGGCACCGGCGAGCACCTCGCCGAACTTGAAAACCTTGCACA
GGGTGATCTGCCGGAGGGCGTCGATGTGCTGATCTGCCCGCCCGCAACGCTCATCTCGCGCGCGGCCGACCGTGCCGGTG
ACATCGCAATTGGCGGGCAGGATTGCCACGCAAAGACGTCTGGCGCACATACTGGCGATCTGAGCGCTGACATGCTTCGG
GATGCGGGGGCAACCTATGTGATCATTGGCCACTCGGAACGCCGCGCCGATCACGGCGAAGGAGACGCGGATGTGCGCGC
CAAGACCGAGGCCGCTCAGGCTGCGGGCCTTGTGGCCGTGGTTTGCATCGGTGAAACGCTCGAGGAGCGCGAGGCTGGCA
CCACCCTAGAGGTGGTTGGCGCGCAGCTCGCGGGCTCCCTGCCCGATGGTGTCACGGCGGAGAACACAGTTGTCGCCTAT
GAACCCGTCTGGGCCATCGGCACAGGCAAGGTTCCCACGCTCGATCAGATCGCTGAGGTGCATGACGCTCTGCGTGCAGA
TCTGGTGGCGCGCTTTGGTGCAGCGGGCAAGGACCTGCCACTGCTCTATGGTGGTTCCGTGAAACCAGGCAATGCAGCCG
AGATCTTTGGGGTTTCCAACGTCGATGGCGCGCTTGTGGGTGGCGCCAGCCTAAAGGCTGCGGACTTCGGCCCCATCATT
GCGGCCCTCGCCGCAAGCTGA

Upstream 100 bases:

>100_bases
CGCCCTTTTTTAATGTCGCGTGTGGCGGTGATTGCTCGCCTCGCGCCCCGGTGCTACCACCTGTGGAGACACTTGATCAG
AACAGGATTTGGGGGGCGAC

Downstream 100 bases:

>100_bases
TCCCCCATAAAATGAGTCCGTCGCAAACGAGGTCGCGCAGGCCTTGTTTGCGGTCGCGCCCCACCTATCGATCAAATACG
TGGGCCTCTGACCAGACCTC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MARKLAAGNWKMNGTGEHLAELENLAQGDLPEGVDVLICPPATLISRAADRAGDIAIGGQDCHAKTSGAHTGDLSADMLR
DAGATYVIIGHSERRADHGEGDADVRAKTEAAQAAGLVAVVCIGETLEEREAGTTLEVVGAQLAGSLPDGVTAENTVVAY
EPVWAIGTGKVPTLDQIAEVHDALRADLVARFGAAGKDLPLLYGGSVKPGNAAEIFGVSNVDGALVGGASLKAADFGPII
AALAAS

Sequences:

>Translated_246_residues
MARKLAAGNWKMNGTGEHLAELENLAQGDLPEGVDVLICPPATLISRAADRAGDIAIGGQDCHAKTSGAHTGDLSADMLR
DAGATYVIIGHSERRADHGEGDADVRAKTEAAQAAGLVAVVCIGETLEEREAGTTLEVVGAQLAGSLPDGVTAENTVVAY
EPVWAIGTGKVPTLDQIAEVHDALRADLVARFGAAGKDLPLLYGGSVKPGNAAEIFGVSNVDGALVGGASLKAADFGPII
AALAAS
>Mature_245_residues
ARKLAAGNWKMNGTGEHLAELENLAQGDLPEGVDVLICPPATLISRAADRAGDIAIGGQDCHAKTSGAHTGDLSADMLRD
AGATYVIIGHSERRADHGEGDADVRAKTEAAQAAGLVAVVCIGETLEEREAGTTLEVVGAQLAGSLPDGVTAENTVVAYE
PVWAIGTGKVPTLDQIAEVHDALRADLVARFGAAGKDLPLLYGGSVKPGNAAEIFGVSNVDGALVGGASLKAADFGPIIA
ALAAS

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=44.3089430894309, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=44.3089430894309, Blast_Score=171, Evalue=7e-43,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=45.3815261044177, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17536593, Length=248, Percent_Identity=44.758064516129, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6320255, Length=245, Percent_Identity=43.6734693877551, Blast_Score=189, Evalue=3e-49,
Organism=Drosophila melanogaster, GI28572004, Length=248, Percent_Identity=45.9677419354839, Blast_Score=183, Evalue=9e-47,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=45.9349593495935, Blast_Score=183, Evalue=9e-47,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=45.9349593495935, Blast_Score=183, Evalue=9e-47,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_SILST (Q1GIL9)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612759.1
- ProteinModelPortal:   Q1GIL9
- SMR:   Q1GIL9
- STRING:   Q1GIL9
- GeneID:   4078076
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0764
- NMPDR:   fig|292414.1.peg.2867
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   RAYHRES
- PhylomeDB:   Q1GIL9
- BioCyc:   SSP292414:TM1040_0764-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 24837; Mature: 24706

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 91-91 ACT_SITE 161-161 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKLAAGNWKMNGTGEHLAELENLAQGDLPEGVDVLICPPATLISRAADRAGDIAIGGQ
CCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCEEECCC
DCHAKTSGAHTGDLSADMLRDAGATYVIIGHSERRADHGEGDADVRAKTEAAQAAGLVAV
CCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCHHCCCCCCCCCCHHHHHHHHHHCCEEEE
VCIGETLEEREAGTTLEVVGAQLAGSLPDGVTAENTVVAYEPVWAIGTGKVPTLDQIAEV
EEECCHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCEEEEECCEEEECCCCCCCHHHHHHH
HDALRADLVARFGAAGKDLPLLYGGSVKPGNAAEIFGVSNVDGALVGGASLKAADFGPII
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCCCCCEEECCCCCCCCCHHHHH
AALAAS
HHHHCC
>Mature Secondary Structure 
ARKLAAGNWKMNGTGEHLAELENLAQGDLPEGVDVLICPPATLISRAADRAGDIAIGGQ
CCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCEEECCC
DCHAKTSGAHTGDLSADMLRDAGATYVIIGHSERRADHGEGDADVRAKTEAAQAAGLVAV
CCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCHHCCCCCCCCCCHHHHHHHHHHCCEEEE
VCIGETLEEREAGTTLEVVGAQLAGSLPDGVTAENTVVAYEPVWAIGTGKVPTLDQIAEV
EEECCHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCEEEEECCEEEECCCCCCCHHHHHHH
HDALRADLVARFGAAGKDLPLLYGGSVKPGNAAEIFGVSNVDGALVGGASLKAADFGPII
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCCCCCEEECCCCCCCCCHHHHH
AALAAS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA