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The map label for this gene is iscA [H]

Identifier: 99080606

GI number: 99080606

Start: 821644

End: 821997

Strand: Reverse

Name: iscA [H]

Synonym: TM1040_0765

Alternate gene names: 99080606

Gene position: 821997-821644 (Counterclockwise)

Preceding gene: 99080607

Following gene: 99080605

Centisome position: 25.68

GC content: 58.76

Gene sequence:

>354_bases
ATGTTCGCCATTCCCGGCAAGCAAGCTGTTACCATGACCCCCCGAGCCGCCGCGCAGATCTCCAAACTCATGGAGAACGC
AGGCCATGCCGGTCTGCGGATCGGCGTCAAGAAAGGCGGCTGTGCGGGCATGGAGTACACGATGGAATATGTCGACGTCG
CCGATCCGAACGATGAGGTGGTGGAGCAGGACGGCGCGCGGGTGCTGATCGCCCCGATGGCCCAGATGTTCCTCTTTGGC
ACCGAAATCGACTATGAGACGACGCTTCTTGAGTCGAGTTTTAAGTTCAACAACCCCAATGTGGCCGAAGCCTGTGGCTG
CGGCGAGTCGATCAGTTTCAAGGTCGGCGAATAA

Upstream 100 bases:

>100_bases
ATCGTGATCTGAGACTGTCGCCTTTCCCTGACATCCGTGCCCCTTGATGGGCTACGCCACACCACCTAGGTTACAGGGCA
ACACACAACGGAGCACCCCC

Downstream 100 bases:

>100_bases
TACTGCCCCTTATTCTGACGAGACCCCAAAATAGGGTGGCTTTGCGCCGCCCTTTTTTAATGTCGCGTGTGGCGGTGATT
GCTCGCCTCGCGCCCCGGTG

Product: HesB/YadR/YfhF

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MFAIPGKQAVTMTPRAAAQISKLMENAGHAGLRIGVKKGGCAGMEYTMEYVDVADPNDEVVEQDGARVLIAPMAQMFLFG
TEIDYETTLLESSFKFNNPNVAEACGCGESISFKVGE

Sequences:

>Translated_117_residues
MFAIPGKQAVTMTPRAAAQISKLMENAGHAGLRIGVKKGGCAGMEYTMEYVDVADPNDEVVEQDGARVLIAPMAQMFLFG
TEIDYETTLLESSFKFNNPNVAEACGCGESISFKVGE
>Mature_117_residues
MFAIPGKQAVTMTPRAAAQISKLMENAGHAGLRIGVKKGGCAGMEYTMEYVDVADPNDEVVEQDGARVLIAPMAQMFLFG
TEIDYETTLLESSFKFNNPNVAEACGCGESISFKVGE

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=110, Percent_Identity=45.4545454545455, Blast_Score=109, Evalue=6e-25,
Organism=Homo sapiens, GI160420328, Length=98, Percent_Identity=37.7551020408163, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788877, Length=104, Percent_Identity=42.3076923076923, Blast_Score=96, Evalue=6e-22,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=41.9047619047619, Blast_Score=91, Evalue=3e-20,
Organism=Escherichia coli, GI1786351, Length=103, Percent_Identity=33.0097087378641, Blast_Score=69, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25146411, Length=108, Percent_Identity=46.2962962962963, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI193209025, Length=108, Percent_Identity=46.2962962962963, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71998452, Length=113, Percent_Identity=30.9734513274336, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323001, Length=101, Percent_Identity=35.6435643564356, Blast_Score=82, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24642263, Length=111, Percent_Identity=45.045045045045, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 12529; Mature: 12529

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAIPGKQAVTMTPRAAAQISKLMENAGHAGLRIGVKKGGCAGMEYTMEYVDVADPNDEV
CCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCHHH
VEQDGARVLIAPMAQMFLFGTEIDYETTLLESSFKFNNPNVAEACGCGESISFKVGE
HHCCCCEEEEHHHHHHHHHCCCCCCHHHEECCCCCCCCCCHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MFAIPGKQAVTMTPRAAAQISKLMENAGHAGLRIGVKKGGCAGMEYTMEYVDVADPNDEV
CCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCHHH
VEQDGARVLIAPMAQMFLFGTEIDYETTLLESSFKFNNPNVAEACGCGESISFKVGE
HHCCCCEEEEHHHHHHHHHCCCCCCHHHEECCCCCCCCCCHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]