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The map label for this gene is narK [H]

Identifier: 94971858

GI number: 94971858

Start: 102082

End: 103317

Strand: Direct

Name: narK [H]

Synonym: Dgeo_2389

Alternate gene names: 94971858

Gene position: 102082-103317 (Clockwise)

Preceding gene: 94971859

Following gene: 94971857

Centisome position: 17.78

GC content: 67.39

Gene sequence:

>1236_bases
ATGACCCAGCCTGCCAACATCCCGCTTCCCGCTCCCGCCCTCACCGCCGATGCCCGGCGCGTCGTCACCTGGAGCACCCT
CGGCTTCACCCTGATGTTCGCCGTGTGGGTGATGTTCTCGATCGTGGCCCTGCCCATCCGCAAGCAGCTCGGGCTGACGG
ACGCGCAGTTCACGCTGCTGACGGCCATCCCGGTGCTCACGGGATCGCTGCTGCGCCTGCCCGCCGGGCTGCTGGCCGAC
CGACTTGGCGGCAAGAAGATGTTCCTGACCGTGACGCTGGTGACTGCCGCCTTCTCGCTGGCGCTCTCGTTTGCCCAGGC
GTACGACACGCTGCTTGTCCTTGCCCTCGGGGTGGGGCTGGCGGGGGTGAGCTTCGCGGTGGGGAACGCCTGGATTGCCC
AGTGGGTGCCCGCCTCACGGCAGGGGCTCGCGCTGGGGACCTTCGGCGCGGGAAATGCGGGAGCCAGCATCACCAAGCTG
ATTGCGCCGCTGCTGATCACGCTGGTGCCCGCCGGGCTGCTGATTCCGGGAGGCTGGCACTTCGTCCCCTTCGTGTTCGC
CCTGATGCTTGTGCTGTGCGCGGGACTCACCGCCCGTTTCACCCCTGCCGACGGTGCCGTCCGTCCCCAGCGCTCCCTGG
CCGACTGGCTGCGGCCCCTGGGAAACGTGCAGGTCTGGCGCTTCGGGCTGTACTACGTGGTGTTCTTCGGCGCCTATGTG
GCCCTGAGCCTCTTCCTGCCGAAGTACTACGTGGACCACTACGAGGTTCCCCTGACGGAAGCCGGCCTGCTCACCGCCCT
CTTCATCTTCCCGGCCAGCCTGCTGCGGCCCCTGGGCGGCTACCTGAGTGACCGCTTTGGGCCGCGCGGGGTGACGATTG
CCTCCTTCGCCGTGATGCTCTTGGGCCTTTTGCCCCTGACTCACGAGCTGCCGCTTGTCACCTTTCTGGCGCTGACCACT
GTTGTTGGGGTTGGCATGGGCGTCGGCAAGGCAAGCACGTACACGCTGGTCGCACAGTGGTATCCCGGACAGATGGGCGT
CGTGGGCGGTTTGGTCGGGCTGCTGGGCGGACTGGGCGGCTTTATCCTCCCCCTCGCCTTCGCGACTCTGAAGCCTGTGT
TGGGCGCTCAGGCGGCCTTCATCACCCTGCTGGCCGTGACGCTCGTCAGTACCGTGATCTTCCTGGGGAGCATGCTGCGC
CTTCGGGCACTGGGACGGCGGCCCAACTTTGCGTAG

Upstream 100 bases:

>100_bases
AGCGCAGCCCGCCCGAATCGCCCCGCTGGAGGGGGGAGTGCCTATCTGACCGCTTCCCCTCCTGCCCCCAGGCCCTCACT
CCTTCCCCCGAGGTTCCCCC

Downstream 100 bases:

>100_bases
GAAAGTTGTGGCAAGTGGTTGGGGTAGGGGAGCGTGGGACTGAGTCGGGCACCGCCATGCAAGCCGCCTGCAGGTCAAGC
CATTCCTGTTGCTCCAGCCA

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTQPANIPLPAPALTADARRVVTWSTLGFTLMFAVWVMFSIVALPIRKQLGLTDAQFTLLTAIPVLTGSLLRLPAGLLAD
RLGGKKMFLTVTLVTAAFSLALSFAQAYDTLLVLALGVGLAGVSFAVGNAWIAQWVPASRQGLALGTFGAGNAGASITKL
IAPLLITLVPAGLLIPGGWHFVPFVFALMLVLCAGLTARFTPADGAVRPQRSLADWLRPLGNVQVWRFGLYYVVFFGAYV
ALSLFLPKYYVDHYEVPLTEAGLLTALFIFPASLLRPLGGYLSDRFGPRGVTIASFAVMLLGLLPLTHELPLVTFLALTT
VVGVGMGVGKASTYTLVAQWYPGQMGVVGGLVGLLGGLGGFILPLAFATLKPVLGAQAAFITLLAVTLVSTVIFLGSMLR
LRALGRRPNFA

Sequences:

>Translated_411_residues
MTQPANIPLPAPALTADARRVVTWSTLGFTLMFAVWVMFSIVALPIRKQLGLTDAQFTLLTAIPVLTGSLLRLPAGLLAD
RLGGKKMFLTVTLVTAAFSLALSFAQAYDTLLVLALGVGLAGVSFAVGNAWIAQWVPASRQGLALGTFGAGNAGASITKL
IAPLLITLVPAGLLIPGGWHFVPFVFALMLVLCAGLTARFTPADGAVRPQRSLADWLRPLGNVQVWRFGLYYVVFFGAYV
ALSLFLPKYYVDHYEVPLTEAGLLTALFIFPASLLRPLGGYLSDRFGPRGVTIASFAVMLLGLLPLTHELPLVTFLALTT
VVGVGMGVGKASTYTLVAQWYPGQMGVVGGLVGLLGGLGGFILPLAFATLKPVLGAQAAFITLLAVTLVSTVIFLGSMLR
LRALGRRPNFA
>Mature_410_residues
TQPANIPLPAPALTADARRVVTWSTLGFTLMFAVWVMFSIVALPIRKQLGLTDAQFTLLTAIPVLTGSLLRLPAGLLADR
LGGKKMFLTVTLVTAAFSLALSFAQAYDTLLVLALGVGLAGVSFAVGNAWIAQWVPASRQGLALGTFGAGNAGASITKLI
APLLITLVPAGLLIPGGWHFVPFVFALMLVLCAGLTARFTPADGAVRPQRSLADWLRPLGNVQVWRFGLYYVVFFGAYVA
LSLFLPKYYVDHYEVPLTEAGLLTALFIFPASLLRPLGGYLSDRFGPRGVTIASFAVMLLGLLPLTHELPLVTFLALTTV
VGVGMGVGKASTYTLVAQWYPGQMGVVGGLVGLLGGLGGFILPLAFATLKPVLGAQAAFITLLAVTLVSTVIFLGSMLRL
RALGRRPNFA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=418, Percent_Identity=24.8803827751196, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787743, Length=431, Percent_Identity=25.9860788863109, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43385; Mature: 43253

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPANIPLPAPALTADARRVVTWSTLGFTLMFAVWVMFSIVALPIRKQLGLTDAQFTLL
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TAIPVLTGSLLRLPAGLLADRLGGKKMFLTVTLVTAAFSLALSFAQAYDTLLVLALGVGL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGVSFAVGNAWIAQWVPASRQGLALGTFGAGNAGASITKLIAPLLITLVPAGLLIPGGWH
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
FVPFVFALMLVLCAGLTARFTPADGAVRPQRSLADWLRPLGNVQVWRFGLYYVVFFGAYV
HHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ALSLFLPKYYVDHYEVPLTEAGLLTALFIFPASLLRPLGGYLSDRFGPRGVTIASFAVML
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LGLLPLTHELPLVTFLALTTVVGVGMGVGKASTYTLVAQWYPGQMGVVGGLVGLLGGLGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
FILPLAFATLKPVLGAQAAFITLLAVTLVSTVIFLGSMLRLRALGRRPNFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TQPANIPLPAPALTADARRVVTWSTLGFTLMFAVWVMFSIVALPIRKQLGLTDAQFTLL
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TAIPVLTGSLLRLPAGLLADRLGGKKMFLTVTLVTAAFSLALSFAQAYDTLLVLALGVGL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGVSFAVGNAWIAQWVPASRQGLALGTFGAGNAGASITKLIAPLLITLVPAGLLIPGGWH
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
FVPFVFALMLVLCAGLTARFTPADGAVRPQRSLADWLRPLGNVQVWRFGLYYVVFFGAYV
HHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ALSLFLPKYYVDHYEVPLTEAGLLTALFIFPASLLRPLGGYLSDRFGPRGVTIASFAVML
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LGLLPLTHELPLVTFLALTTVVGVGMGVGKASTYTLVAQWYPGQMGVVGGLVGLLGGLGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
FILPLAFATLKPVLGAQAAFITLLAVTLVSTVIFLGSMLRLRALGRRPNFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]