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The map label for this gene is nasC [H]

Identifier: 94971859

GI number: 94971859

Start: 99901

End: 102030

Strand: Direct

Name: nasC [H]

Synonym: Dgeo_2390

Alternate gene names: 94971859

Gene position: 99901-102030 (Clockwise)

Preceding gene: 94971864

Following gene: 94971858

Centisome position: 17.4

GC content: 66.67

Gene sequence:

>2130_bases
ATGCCGAGCACCCCCACTCCCCGGCGCGCGTCAGCCACACGCGTGGTGCGGACAACTTGTCCATATTGCGCAGTCCAGTG
CAACTTCGACCTACAGATCGAGGACAACCTGCCCGTCAAGGCGACACCGACCAAGGAATGCCCCGTGGCCCACGGCACCG
TCTGTAAGAAGGGGCTCTCCGCCCTGAATGACCTGCGTCACCCTGACCGCCTGACTCAGCCCCTGCTCCGCGAGAATGGC
GAACTTGTGCCTGTGAGCTGGACTGAGGCGTTGGCCTACGTGTCGGACGCGCTGGGGCCCCTCCTCGCTACGCAGCCAAC
TGCGGTGGGCGTATTGGGCAGCGGCGCGCTGACAAACGAAAAAACTTACCTGCTAGGTAAGTTCGCCCGCCTGGCGCTGA
GAACGGCAAATATTGACTACAACGGGCGCTATTGCATGTCCTCGGCTGCCACAGCGCTGAACCGCACGGTGGGCTATGAC
CGGGGCCTGGGCTTTCCGCTGGAGGAGATCAGTACAAGTGACCTGATCCTGCTCGTGGGCGCGAACATCGCCGAGACGCT
GCCGCCGGTGATGCAGTACCTCAAGCGGGCGCGAGACCGGGGCGCGACCGTGTACGCCATCGATCCGCGTGCTACCCCGA
CGGCGAAGGTGGCCGGGCATCACCTTGCTGTCCGCCCCGGCACCGACGGCATCCTTGCGCTTGGCCTGCTGCACCTGATG
AAGGCGTGGGGGCGCATTCGCCCGACTGCCCCCGCCCACGGCATGCCGGAGGTGCTGTGGCAGGCCGACGACTACCCGCC
CGCCTCTGTGGCCAGCGCGTGCGGTGTCGCAGAAGACGAGCTGATGACCCTAGCGAAGCTCTACGCCAACGCTCATAAGC
CGCTGATCCTGACTGGACGCGGTCCCGAACAGCATGCCTACGGCACCGATACCGTCCAGGCTTACCTGAATCTCGCTTTC
CTGACCGGGCACTTCGGCAAGCCGGGCGGCGGCTACGGCACGCTCACCGGGCAGGGCAACGGCCAGGGGGGGCGTGAGCA
CGGGCAGAAGGCGGACCAGCTTCCCGGCGCGCGCAGCCTGCGCAATCCCAAACACCGCAGGGAGATCGCCGCCCTGTGGG
GGTGCTTACCCGAGGACCTGCCTCAACCCGGCCTGAGCGCCCAAGAGTTGCTCAATGCCTGCGGTGAGGCGGGCGGCATT
GAAGCTTTGATTGTGCTGGGATCGAACCCCATCGTCAGCGCGCCCGGAGCCCTGCAGGTTCGTCAACGCCTCAGTGCCCT
CAAGCACCTCATCGTCATTGACGTTCTGCCCAGTGAGACCGCACAGCTTGCCACACTCGTCCTCCCCGGCTCCATGTGGT
GCGAGGAGGAGGGCACAACCACCAACCTCGAAGGCCGGGTACAGCGCCGGCGCCAGGCAATCACTCCCCCCGCTGCGGCC
CGTGAAGACTGGCGCATCCTCTGCGACCTGGCCGCAGCGATTGGGCGCCCTCAGGGATTCACCTACGCCACCTTCCGTGA
GCTTCAGGATGAATTCTTCCGTGCAACAGGGGGCGGCGTGGCTGACTACAGCGGCCTCAGCGCTGAACGCCTCGATCGGG
CAAGCGCCCAGTGGCCCGTTCGCAGTGCCGACGGTCCCGACACTCCCTACGCCTACGCGCCGAAGTACCCGACACCGGAC
GGCCTCGCCAGGCTACACGTCCCGAGCTTTCCGCCGCTCCCTTTGTCCCACCACCTGCTCCTCACGACTGGGCGGCTGGG
CAACCAATACCAGAGTGGCACCCAGACCCGCCGCAATCCCGCTCTGCGGGCCACCCTCGAACTCCAGGTTCACCCAGAGA
CGGCCCGTGAGCGCAGCCTGAAACCCGGCGACCTCGCCCGCGTGACCACCTCGCACGGCACACTCGACCTGCCCGTCGCG
CTCAACCCCGGCCTGCGTCCCGACACCTTGTTCATGCCCTTCCACTGGGAAGCAAGCGCGAACCTGCTGACCAGCCCTGA
TCGCCTCGATCCCCACTCGCGGATGCCCGCCTTCAAGGCGACGCCCGCCACCCTCATGCCCGTTCCCGTCTCCGTTCCTG
AAGCGCAGCCCGCCCGAATCGCCCCGCTGGAGGGGGGAGTGCCTATCTGA

Upstream 100 bases:

>100_bases
TCGGATATTTGTGGCCTATACTTGCAACTTGTCTAAAAATTTGGAAACAAATTGCATTTTTGGCCTTGACGACCCTGTCG
ATCCCTGCCAAGGTGCGCGC

Downstream 100 bases:

>100_bases
CCGCTTCCCCTCCTGCCCCCAGGCCCTCACTCCTTCCCCCGAGGTTCCCCCATGACCCAGCCTGCCAACATCCCGCTTCC
CGCTCCCGCCCTCACCGCCG

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 709; Mature: 708

Protein sequence:

>709_residues
MPSTPTPRRASATRVVRTTCPYCAVQCNFDLQIEDNLPVKATPTKECPVAHGTVCKKGLSALNDLRHPDRLTQPLLRENG
ELVPVSWTEALAYVSDALGPLLATQPTAVGVLGSGALTNEKTYLLGKFARLALRTANIDYNGRYCMSSAATALNRTVGYD
RGLGFPLEEISTSDLILLVGANIAETLPPVMQYLKRARDRGATVYAIDPRATPTAKVAGHHLAVRPGTDGILALGLLHLM
KAWGRIRPTAPAHGMPEVLWQADDYPPASVASACGVAEDELMTLAKLYANAHKPLILTGRGPEQHAYGTDTVQAYLNLAF
LTGHFGKPGGGYGTLTGQGNGQGGREHGQKADQLPGARSLRNPKHRREIAALWGCLPEDLPQPGLSAQELLNACGEAGGI
EALIVLGSNPIVSAPGALQVRQRLSALKHLIVIDVLPSETAQLATLVLPGSMWCEEEGTTTNLEGRVQRRRQAITPPAAA
REDWRILCDLAAAIGRPQGFTYATFRELQDEFFRATGGGVADYSGLSAERLDRASAQWPVRSADGPDTPYAYAPKYPTPD
GLARLHVPSFPPLPLSHHLLLTTGRLGNQYQSGTQTRRNPALRATLELQVHPETARERSLKPGDLARVTTSHGTLDLPVA
LNPGLRPDTLFMPFHWEASANLLTSPDRLDPHSRMPAFKATPATLMPVPVSVPEAQPARIAPLEGGVPI

Sequences:

>Translated_709_residues
MPSTPTPRRASATRVVRTTCPYCAVQCNFDLQIEDNLPVKATPTKECPVAHGTVCKKGLSALNDLRHPDRLTQPLLRENG
ELVPVSWTEALAYVSDALGPLLATQPTAVGVLGSGALTNEKTYLLGKFARLALRTANIDYNGRYCMSSAATALNRTVGYD
RGLGFPLEEISTSDLILLVGANIAETLPPVMQYLKRARDRGATVYAIDPRATPTAKVAGHHLAVRPGTDGILALGLLHLM
KAWGRIRPTAPAHGMPEVLWQADDYPPASVASACGVAEDELMTLAKLYANAHKPLILTGRGPEQHAYGTDTVQAYLNLAF
LTGHFGKPGGGYGTLTGQGNGQGGREHGQKADQLPGARSLRNPKHRREIAALWGCLPEDLPQPGLSAQELLNACGEAGGI
EALIVLGSNPIVSAPGALQVRQRLSALKHLIVIDVLPSETAQLATLVLPGSMWCEEEGTTTNLEGRVQRRRQAITPPAAA
REDWRILCDLAAAIGRPQGFTYATFRELQDEFFRATGGGVADYSGLSAERLDRASAQWPVRSADGPDTPYAYAPKYPTPD
GLARLHVPSFPPLPLSHHLLLTTGRLGNQYQSGTQTRRNPALRATLELQVHPETARERSLKPGDLARVTTSHGTLDLPVA
LNPGLRPDTLFMPFHWEASANLLTSPDRLDPHSRMPAFKATPATLMPVPVSVPEAQPARIAPLEGGVPI
>Mature_708_residues
PSTPTPRRASATRVVRTTCPYCAVQCNFDLQIEDNLPVKATPTKECPVAHGTVCKKGLSALNDLRHPDRLTQPLLRENGE
LVPVSWTEALAYVSDALGPLLATQPTAVGVLGSGALTNEKTYLLGKFARLALRTANIDYNGRYCMSSAATALNRTVGYDR
GLGFPLEEISTSDLILLVGANIAETLPPVMQYLKRARDRGATVYAIDPRATPTAKVAGHHLAVRPGTDGILALGLLHLMK
AWGRIRPTAPAHGMPEVLWQADDYPPASVASACGVAEDELMTLAKLYANAHKPLILTGRGPEQHAYGTDTVQAYLNLAFL
TGHFGKPGGGYGTLTGQGNGQGGREHGQKADQLPGARSLRNPKHRREIAALWGCLPEDLPQPGLSAQELLNACGEAGGIE
ALIVLGSNPIVSAPGALQVRQRLSALKHLIVIDVLPSETAQLATLVLPGSMWCEEEGTTTNLEGRVQRRRQAITPPAAAR
EDWRILCDLAAAIGRPQGFTYATFRELQDEFFRATGGGVADYSGLSAERLDRASAQWPVRSADGPDTPYAYAPKYPTPDG
LARLHVPSFPPLPLSHHLLLTTGRLGNQYQSGTQTRRNPALRATLELQVHPETARERSLKPGDLARVTTSHGTLDLPVAL
NPGLRPDTLFMPFHWEASANLLTSPDRLDPHSRMPAFKATPATLMPVPVSVPEAQPARIAPLEGGVPI

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=698, Percent_Identity=28.3667621776504, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1788534, Length=799, Percent_Identity=27.2841051314143, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1787870, Length=505, Percent_Identity=24.5544554455446, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI171474008, Length=515, Percent_Identity=25.4368932038835, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI87081797, Length=751, Percent_Identity=22.9027962716378, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787778, Length=388, Percent_Identity=27.0618556701031, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI3868720, Length=256, Percent_Identity=28.515625, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI3868719, Length=278, Percent_Identity=25.1798561151079, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI87081994, Length=513, Percent_Identity=23.0019493177388, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 76057; Mature: 75925

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTPTPRRASATRVVRTTCPYCAVQCNFDLQIEDNLPVKATPTKECPVAHGTVCKKGLS
CCCCCCCCCCCHHHHHEECCCEEEEEECEEEEECCCCCEECCCCCCCCCCCHHHHHHHHH
ALNDLRHPDRLTQPLLRENGELVPVSWTEALAYVSDALGPLLATQPTAVGVLGSGALTNE
HHHHCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
KTYLLGKFARLALRTANIDYNGRYCMSSAATALNRTVGYDRGLGFPLEEISTSDLILLVG
CHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEC
ANIAETLPPVMQYLKRARDRGATVYAIDPRATPTAKVAGHHLAVRPGTDGILALGLLHLM
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCEEEECCCCCHHHHHHHHHHH
KAWGRIRPTAPAHGMPEVLWQADDYPPASVASACGVAEDELMTLAKLYANAHKPLILTGR
HHHCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECC
GPEQHAYGTDTVQAYLNLAFLTGHFGKPGGGYGTLTGQGNGQGGREHGQKADQLPGARSL
CCCCCCCCHHHHHHHHHHHHEECCCCCCCCCEEEEECCCCCCCCHHHCCHHHHCCCHHHH
RNPKHRREIAALWGCLPEDLPQPGLSAQELLNACGEAGGIEALIVLGSNPIVSAPGALQV
CCHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHH
RQRLSALKHLIVIDVLPSETAQLATLVLPGSMWCEEEGTTTNLEGRVQRRRQAITPPAAA
HHHHHHHHHEEEEEECCCCCHHEEEEEECCCCEECCCCCCCCHHHHHHHHHHHCCCCCCC
REDWRILCDLAAAIGRPQGFTYATFRELQDEFFRATGGGVADYSGLSAERLDRASAQWPV
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCC
RSADGPDTPYAYAPKYPTPDGLARLHVPSFPPLPLSHHLLLTTGRLGNQYQSGTQTRRNP
CCCCCCCCCCEECCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHCCCCHHCCC
ALRATLELQVHPETARERSLKPGDLARVTTSHGTLDLPVALNPGLRPDTLFMPFHWEASA
CEEEEEEEEECCCHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEEEECCCC
NLLTSPDRLDPHSRMPAFKATPATLMPVPVSVPEAQPARIAPLEGGVPI
CEECCCCCCCCHHCCCCCCCCCCEEEECCCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure 
PSTPTPRRASATRVVRTTCPYCAVQCNFDLQIEDNLPVKATPTKECPVAHGTVCKKGLS
CCCCCCCCCCHHHHHEECCCEEEEEECEEEEECCCCCEECCCCCCCCCCCHHHHHHHHH
ALNDLRHPDRLTQPLLRENGELVPVSWTEALAYVSDALGPLLATQPTAVGVLGSGALTNE
HHHHCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
KTYLLGKFARLALRTANIDYNGRYCMSSAATALNRTVGYDRGLGFPLEEISTSDLILLVG
CHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEC
ANIAETLPPVMQYLKRARDRGATVYAIDPRATPTAKVAGHHLAVRPGTDGILALGLLHLM
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCEEEECCCCCHHHHHHHHHHH
KAWGRIRPTAPAHGMPEVLWQADDYPPASVASACGVAEDELMTLAKLYANAHKPLILTGR
HHHCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECC
GPEQHAYGTDTVQAYLNLAFLTGHFGKPGGGYGTLTGQGNGQGGREHGQKADQLPGARSL
CCCCCCCCHHHHHHHHHHHHEECCCCCCCCCEEEEECCCCCCCCHHHCCHHHHCCCHHHH
RNPKHRREIAALWGCLPEDLPQPGLSAQELLNACGEAGGIEALIVLGSNPIVSAPGALQV
CCHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHH
RQRLSALKHLIVIDVLPSETAQLATLVLPGSMWCEEEGTTTNLEGRVQRRRQAITPPAAA
HHHHHHHHHEEEEEECCCCCHHEEEEEECCCCEECCCCCCCCHHHHHHHHHHHCCCCCCC
REDWRILCDLAAAIGRPQGFTYATFRELQDEFFRATGGGVADYSGLSAERLDRASAQWPV
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCC
RSADGPDTPYAYAPKYPTPDGLARLHVPSFPPLPLSHHLLLTTGRLGNQYQSGTQTRRNP
CCCCCCCCCCEECCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHCCCCHHCCC
ALRATLELQVHPETARERSLKPGDLARVTTSHGTLDLPVALNPGLRPDTLFMPFHWEASA
CEEEEEEEEECCCHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEEEECCCC
NLLTSPDRLDPHSRMPAFKATPATLMPVPVSVPEAQPARIAPLEGGVPI
CEECCCCCCCCHHCCCCCCCCCCEEEECCCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 7868621 [H]