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The map label for this gene is insB1 [H]

Identifier: 94971852

GI number: 94971852

Start: 108235

End: 108681

Strand: Direct

Name: insB1 [H]

Synonym: Dgeo_2383

Alternate gene names: 94971852

Gene position: 108235-108681 (Clockwise)

Preceding gene: 94971854

Following gene: 94971850

Centisome position: 18.85

GC content: 60.4

Gene sequence:

>447_bases
GTGACGCAGACCCCTGGGGTCTGCGTCACTCCCCCAGACGAGGTGGTTGTTGAACTGGACGAGTTGTGGACCTTCGTGGG
CAAGAAAAAGCAGGCGAGGTGGTTGTGGATCGCCCTAGAGCGCCGCACCCGAAAGGTGCTGGCTTGGGTCCTTGGAGACC
GCAGCGAAGCGACCGCTTTCAAGTTGTGGGACCGCTTGCCCCTCTCCCCAGAGCAGCGCCTGAAAGGCACTTTTTGCACG
GACCTCTGGCGTGCCTATGATGAACCGTTGTTGGGCGTCAAACGGCTGACCCGCCGAGGGGACACCAACCATGTCGAGCG
GCTGAACTGCACCTTGAGACAACGCCTGGGTCGTCTGGTCCGCAAGTCCCTCTCGTTCTCGAAGTCAGACGAGATGCTCG
AAGCTTCACTTACCCTGGCCTTCCACCGCTACAACCTGTCACGTTGA

Upstream 100 bases:

>100_bases
TCGATGCCGTCCACGAACGGATGAGTCTCAGGGGCGTGCAGCGCGTCTTTGGCGTCCACCGCAACACGGTCATCCGGTGG
ATAAAAAGGGGGCCGCCGAA

Downstream 100 bases:

>100_bases
CGCAGCCACTACCTCCCTTTGCTCCGGTAGGCGGAACAATATGCGCTGCGTCGCCGCCGATAAACAGCCGTCCGTGCTGC
ATGCGGTCACACACAAAAGA

Product: IS1 transposase

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MTQTPGVCVTPPDEVVVELDELWTFVGKKKQARWLWIALERRTRKVLAWVLGDRSEATAFKLWDRLPLSPEQRLKGTFCT
DLWRAYDEPLLGVKRLTRRGDTNHVERLNCTLRQRLGRLVRKSLSFSKSDEMLEASLTLAFHRYNLSR

Sequences:

>Translated_148_residues
MTQTPGVCVTPPDEVVVELDELWTFVGKKKQARWLWIALERRTRKVLAWVLGDRSEATAFKLWDRLPLSPEQRLKGTFCT
DLWRAYDEPLLGVKRLTRRGDTNHVERLNCTLRQRLGRLVRKSLSFSKSDEMLEASLTLAFHRYNLSR
>Mature_147_residues
TQTPGVCVTPPDEVVVELDELWTFVGKKKQARWLWIALERRTRKVLAWVLGDRSEATAFKLWDRLPLSPEQRLKGTFCTD
LWRAYDEPLLGVKRLTRRGDTNHVERLNCTLRQRLGRLVRKSLSFSKSDEMLEASLTLAFHRYNLSR

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1787223, Length=126, Percent_Identity=41.2698412698413, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1786470, Length=125, Percent_Identity=41.6, Blast_Score=79, Evalue=8e-17,
Organism=Escherichia coli, GI1786459, Length=125, Percent_Identity=41.6, Blast_Score=79, Evalue=8e-17,
Organism=Escherichia coli, GI1789853, Length=126, Percent_Identity=40.4761904761905, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1788203, Length=126, Percent_Identity=40.4761904761905, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1786203, Length=126, Percent_Identity=40.4761904761905, Blast_Score=78, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 17342; Mature: 17211

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTPGVCVTPPDEVVVELDELWTFVGKKKQARWLWIALERRTRKVLAWVLGDRSEATAF
CCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHH
KLWDRLPLSPEQRLKGTFCTDLWRAYDEPLLGVKRLTRRGDTNHVERLNCTLRQRLGRLV
HHHHCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RKSLSFSKSDEMLEASLTLAFHRYNLSR
HHHHCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQTPGVCVTPPDEVVVELDELWTFVGKKKQARWLWIALERRTRKVLAWVLGDRSEATAF
CCCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHH
KLWDRLPLSPEQRLKGTFCTDLWRAYDEPLLGVKRLTRRGDTNHVERLNCTLRQRLGRLV
HHHHCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RKSLSFSKSDEMLEASLTLAFHRYNLSR
HHHHCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]