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The map label for this gene is pcaB [H]

Identifier: 94971854

GI number: 94971854

Start: 106290

End: 107681

Strand: Direct

Name: pcaB [H]

Synonym: Dgeo_2385

Alternate gene names: 94971854

Gene position: 106290-107681 (Clockwise)

Preceding gene: 94971855

Following gene: 94971852

Centisome position: 18.51

GC content: 69.9

Gene sequence:

>1392_bases
ATGCCGCTGACGCCTGCCGATTCGGCTCTCTTCGGCTCCCTCTTTGCTCAGCCGGAAGTGGCCGCCCTCTTTACCGACGA
GGTCTATGTCCGCCGGTTGCTGACGGTGGAGGGAGCGCTCGCCAGCGTACAGGGCAGGCTGGGAATCATCCCGCCCGAGT
CGGCAGAGGCCATCTTGCACCTCACACAGACGTTCTCTCCCGACTTGGCGTCACTCCGCGCAGGCCTCCTGACGGACGGC
GTGCCGGTGAGTGCCCTGCTTGCCCAGCTGCGCCCGGCTCTTCCCGCCGCCGCGCGTGACCACCTGCACTTTGGCGCGAC
CACCCAGGATATTGTCGACACGGCCTTCGTGCTGGCTGCGCGTGAAGCGCTGAGGGTGCTCCGCCGTGAACTGCTGGCGG
TGGGCGACCGGCTCGCGGAACTCTCTCAGGCACAGGCCCAGACGCTGATGCCGGGCCGCACCCACTCGCAGCAGGCGCTG
CCCGTCACCTTCGGCCTCAAGGCCGCCGGATGGCTCACGCCGCTCACCCGGCACCTGGAGCGGTTGCGAGAGCTGGAAGG
CCGGCTGTACGTGGTGAGTTTCGGGGGAGCCGCGGGCACGCTCGCCGCGCTGGGGGACCGGGGCTTGGCGGTGGCGGACG
CCCTGGCCGCCGAGCTGGATTTGGCGGCTCCCCCCACCCCCTGGCACACCCAGCGGGATACCGTCTTTGAACTGGCCGCC
TGGCTTGCGGGCGTGGGGACCAGTCTGGGCAAGCTCGCCCAGGACGTGATCCTGCTCGCTCAGAGTGAAGTCGGTGAGGT
ACGTGAGGGAGGCGGGGGCGGAGGGTCGAGCACCATGCCTCAGAAGCAAAATCCCATCACCTCGGAGGTGATCCTGGCCG
CAGCCAGCACGAATGCCGGGCTGCTTGCCGCCGTCGCCCGCAGCGGCGTGCAGGAGCACGAACGCGGCACCCACGGCTGG
CAGGTGGAGTGGCTGACCGTGCCGCAGATGCTTGGCCTGATTGGAGCGGCCCTCGCCCATACTCGCCGCCTGCTGGACGG
TCTGGAGGTACAGGCGGCACGGATGGCGCACAACGTCGCGGCCTCGCACGGCCTGATGCTCGCCGAGGCCCTGAGTTTCG
CCCTGACCGCCGTCCTGCCGAAGGAGGAGGCCAAGGCCCTGATTCGGCAGGCGGTCCCGCAGGCGCTTGCGGGTGAGGGC
CATCTTGTCGGCCTCGTTCGGGAGCGCCTGGGACCTCGGGGGGCCCGGGTGGACTGGGCGGCCCTCACCGAGGCACACAC
GCTGGGCAGCACCTTCAACCTGATTGACCGAGCCATCCGCCACTACCGGCAAGTCCGCCCCTCCTGTTCGCCGGCCGAGG
CGCCGCCTCAGTCCAGCGGCAGCCCGACGTAA

Upstream 100 bases:

>100_bases
ATTGCCCAGCGGGAGGAGACGCCGGATGGGACCGTGTACCGCTTCGACTTCCGCATGCAGGGCAAGGACGAGACGGTCTT
CTTCGCCCCATGAGGTTCCC

Downstream 100 bases:

>100_bases
TTTTCTGCAAGGGCGGTGGCCGCGGCCCTTGAGTGGACGACATAGTCGAGGTCGGCCAGGCGGATGCGCTGCTCAAAAGG
GCCTTCGGCAGGGTTGGTGT

Product: fumarate lyase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPLTPADSALFGSLFAQPEVAALFTDEVYVRRLLTVEGALASVQGRLGIIPPESAEAILHLTQTFSPDLASLRAGLLTDG
VPVSALLAQLRPALPAAARDHLHFGATTQDIVDTAFVLAAREALRVLRRELLAVGDRLAELSQAQAQTLMPGRTHSQQAL
PVTFGLKAAGWLTPLTRHLERLRELEGRLYVVSFGGAAGTLAALGDRGLAVADALAAELDLAAPPTPWHTQRDTVFELAA
WLAGVGTSLGKLAQDVILLAQSEVGEVREGGGGGGSSTMPQKQNPITSEVILAAASTNAGLLAAVARSGVQEHERGTHGW
QVEWLTVPQMLGLIGAALAHTRRLLDGLEVQAARMAHNVAASHGLMLAEALSFALTAVLPKEEAKALIRQAVPQALAGEG
HLVGLVRERLGPRGARVDWAALTEAHTLGSTFNLIDRAIRHYRQVRPSCSPAEAPPQSSGSPT

Sequences:

>Translated_463_residues
MPLTPADSALFGSLFAQPEVAALFTDEVYVRRLLTVEGALASVQGRLGIIPPESAEAILHLTQTFSPDLASLRAGLLTDG
VPVSALLAQLRPALPAAARDHLHFGATTQDIVDTAFVLAAREALRVLRRELLAVGDRLAELSQAQAQTLMPGRTHSQQAL
PVTFGLKAAGWLTPLTRHLERLRELEGRLYVVSFGGAAGTLAALGDRGLAVADALAAELDLAAPPTPWHTQRDTVFELAA
WLAGVGTSLGKLAQDVILLAQSEVGEVREGGGGGGSSTMPQKQNPITSEVILAAASTNAGLLAAVARSGVQEHERGTHGW
QVEWLTVPQMLGLIGAALAHTRRLLDGLEVQAARMAHNVAASHGLMLAEALSFALTAVLPKEEAKALIRQAVPQALAGEG
HLVGLVRERLGPRGARVDWAALTEAHTLGSTFNLIDRAIRHYRQVRPSCSPAEAPPQSSGSPT
>Mature_462_residues
PLTPADSALFGSLFAQPEVAALFTDEVYVRRLLTVEGALASVQGRLGIIPPESAEAILHLTQTFSPDLASLRAGLLTDGV
PVSALLAQLRPALPAAARDHLHFGATTQDIVDTAFVLAAREALRVLRRELLAVGDRLAELSQAQAQTLMPGRTHSQQALP
VTFGLKAAGWLTPLTRHLERLRELEGRLYVVSFGGAAGTLAALGDRGLAVADALAAELDLAAPPTPWHTQRDTVFELAAW
LAGVGTSLGKLAQDVILLAQSEVGEVREGGGGGGSSTMPQKQNPITSEVILAAASTNAGLLAAVARSGVQEHERGTHGWQ
VEWLTVPQMLGLIGAALAHTRRLLDGLEVQAARMAHNVAASHGLMLAEALSFALTAVLPKEEAKALIRQAVPQALAGEGH
LVGLVRERLGPRGARVDWAALTEAHTLGSTFNLIDRAIRHYRQVRPSCSPAEAPPQSSGSPT

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI68303547, Length=276, Percent_Identity=28.9855072463768, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI31541964, Length=276, Percent_Identity=28.9855072463768, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI68303542, Length=276, Percent_Identity=28.9855072463768, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI183227688, Length=294, Percent_Identity=26.8707482993197, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI4557269, Length=294, Percent_Identity=26.8707482993197, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI19743875, Length=323, Percent_Identity=26.9349845201238, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1790398, Length=313, Percent_Identity=28.7539936102236, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787896, Length=205, Percent_Identity=31.7073170731707, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=313, Percent_Identity=25.8785942492013, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17553882, Length=379, Percent_Identity=24.2744063324538, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=432, Percent_Identity=25, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24662684, Length=229, Percent_Identity=27.9475982532751, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI78710009, Length=323, Percent_Identity=24.4582043343653, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24647570, Length=306, Percent_Identity=29.0849673202614, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 48680; Mature: 48549

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTPADSALFGSLFAQPEVAALFTDEVYVRRLLTVEGALASVQGRLGIIPPESAEAILH
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LTQTFSPDLASLRAGLLTDGVPVSALLAQLRPALPAAARDHLHFGATTQDIVDTAFVLAA
HHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHH
REALRVLRRELLAVGDRLAELSQAQAQTLMPGRTHSQQALPVTFGLKAAGWLTPLTRHLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
RLRELEGRLYVVSFGGAAGTLAALGDRGLAVADALAAELDLAAPPTPWHTQRDTVFELAA
HHHHHCCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
WLAGVGTSLGKLAQDVILLAQSEVGEVREGGGGGGSSTMPQKQNPITSEVILAAASTNAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHEEEECCCCCH
LLAAVARSGVQEHERGTHGWQVEWLTVPQMLGLIGAALAHTRRLLDGLEVQAARMAHNVA
HHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASHGLMLAEALSFALTAVLPKEEAKALIRQAVPQALAGEGHLVGLVRERLGPRGARVDWA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHH
ALTEAHTLGSTFNLIDRAIRHYRQVRPSCSPAEAPPQSSGSPT
HHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLTPADSALFGSLFAQPEVAALFTDEVYVRRLLTVEGALASVQGRLGIIPPESAEAILH
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LTQTFSPDLASLRAGLLTDGVPVSALLAQLRPALPAAARDHLHFGATTQDIVDTAFVLAA
HHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHH
REALRVLRRELLAVGDRLAELSQAQAQTLMPGRTHSQQALPVTFGLKAAGWLTPLTRHLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
RLRELEGRLYVVSFGGAAGTLAALGDRGLAVADALAAELDLAAPPTPWHTQRDTVFELAA
HHHHHCCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
WLAGVGTSLGKLAQDVILLAQSEVGEVREGGGGGGSSTMPQKQNPITSEVILAAASTNAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHEEEECCCCCH
LLAAVARSGVQEHERGTHGWQVEWLTVPQMLGLIGAALAHTRRLLDGLEVQAARMAHNVA
HHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASHGLMLAEALSFALTAVLPKEEAKALIRQAVPQALAGEGHLVGLVRERLGPRGARVDWA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHH
ALTEAHTLGSTFNLIDRAIRHYRQVRPSCSPAEAPPQSSGSPT
HHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1390752 [H]