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The map label for this gene is cbiL [H]

Identifier: 94971827

GI number: 94971827

Start: 131295

End: 132053

Strand: Direct

Name: cbiL [H]

Synonym: Dgeo_2358

Alternate gene names: 94971827

Gene position: 131295-132053 (Clockwise)

Preceding gene: 94971828

Following gene: 94971826

Centisome position: 22.87

GC content: 71.01

Gene sequence:

>759_bases
ATGAGCGGCACCTTCTACGGTCTGGGCGTTGGTCCCGGCCCCGGCGGCCTGCTGCCCGTCGCGGCGCTGGAGATCCTCCG
AACTGCCGACCTGATCTACACGCCCCGTTCCCGCGTGTCGCCGGCGTCGGTGGCGCTCGCCGCACTGGACGGCTTGGCCT
TTCCCCGCGAGCGGGTGCGCGAGGTGGAATTCCTGATGGATGGTGACGACGCGCGCCTAGGCGAGCATTACGCCGCGCTG
GCCCGCGAGATCGCCGCGCACCAGCGTGCGGGTCGCCACGTCGCGTACCTGACCATCGGGGACGCGATGACCTACAGCAC
GCTGGGCTACCTGGTGGCCGCCCTGGCCCGCGAGGCGCCGGAGTTGCCCCGCCGCATCCTGCCCGGCGTCACCAGTTACG
CCGCCGCCGCCGCCCGCACCGGCTTCAGCCTGGGTGAGGGCAAGGAACGGGTGCTGATCCTGCCCTGCCCCGACGATATG
GCGGCCCTGCGCGCCGACATTCTCTCGCATGACGTGGTGGTGCTGATGAAGGTGGGGCGGCGGATGCCGGACGTGCTGGC
CCTGCTCGCCGAGCTGGACATTCTCGAACACTGTGCCCTCGCGCACCGCCTTGGCCTGGACGGCGAAGTGGTCCTGCCCT
CGCTCTCGCCTGCCGCCGACTCCCCAATGGGTCGCCGGGAACGGCCTCCCCTCGACGGACGCCTGGGGTACCTGAGCGTG
CTGCTGATTCGCCGGCATCCGCCCCGGAGGTTTTTGTGA

Upstream 100 bases:

>100_bases
GGCAGGTGGCCAAAACCCTGCACGGGCGCGCCCCCGCCGCAGGCCGTGTCCGGGTGGCCCTCTTCGCACTGGACGGGACC
GGGCTGGCGGAGGCGGAGGC

Downstream 100 bases:

>100_bases
AGGTGTATTTCATCGGCGCTGGTCCTGGCGCCCCCGACCTCATCACCGTGCGGGGGGCGCGGCTGCTGGCGCAGGCGGAC
CTCATTCTCTACGCTGGGTC

Product: precorrin-2 C20-methyltransferase

Products: NA

Alternate protein names: S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSGTFYGLGVGPGPGGLLPVAALEILRTADLIYTPRSRVSPASVALAALDGLAFPRERVREVEFLMDGDDARLGEHYAAL
AREIAAHQRAGRHVAYLTIGDAMTYSTLGYLVAALAREAPELPRRILPGVTSYAAAAARTGFSLGEGKERVLILPCPDDM
AALRADILSHDVVVLMKVGRRMPDVLALLAELDILEHCALAHRLGLDGEVVLPSLSPAADSPMGRRERPPLDGRLGYLSV
LLIRRHPPRRFL

Sequences:

>Translated_252_residues
MSGTFYGLGVGPGPGGLLPVAALEILRTADLIYTPRSRVSPASVALAALDGLAFPRERVREVEFLMDGDDARLGEHYAAL
AREIAAHQRAGRHVAYLTIGDAMTYSTLGYLVAALAREAPELPRRILPGVTSYAAAAARTGFSLGEGKERVLILPCPDDM
AALRADILSHDVVVLMKVGRRMPDVLALLAELDILEHCALAHRLGLDGEVVLPSLSPAADSPMGRRERPPLDGRLGYLSV
LLIRRHPPRRFL
>Mature_251_residues
SGTFYGLGVGPGPGGLLPVAALEILRTADLIYTPRSRVSPASVALAALDGLAFPRERVREVEFLMDGDDARLGEHYAALA
REIAAHQRAGRHVAYLTIGDAMTYSTLGYLVAALAREAPELPRRILPGVTSYAAAAARTGFSLGEGKERVLILPCPDDMA
ALRADILSHDVVVLMKVGRRMPDVLALLAELDILEHCALAHRLGLDGEVVLPSLSPAADSPMGRRERPPLDGRLGYLSVL
LIRRHPPRRFL

Specific function: Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.151 [H]

Molecular weight: Translated: 27108; Mature: 26977

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTFYGLGVGPGPGGLLPVAALEILRTADLIYTPRSRVSPASVALAALDGLAFPRERVR
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHH
EVEFLMDGDDARLGEHYAALAREIAAHQRAGRHVAYLTIGDAMTYSTLGYLVAALAREAP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCH
ELPRRILPGVTSYAAAAARTGFSLGEGKERVLILPCPDDMAALRADILSHDVVVLMKVGR
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
RMPDVLALLAELDILEHCALAHRLGLDGEVVLPSLSPAADSPMGRRERPPLDGRLGYLSV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
LLIRRHPPRRFL
HHHHCCCCCCCC
>Mature Secondary Structure 
SGTFYGLGVGPGPGGLLPVAALEILRTADLIYTPRSRVSPASVALAALDGLAFPRERVR
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHH
EVEFLMDGDDARLGEHYAALAREIAAHQRAGRHVAYLTIGDAMTYSTLGYLVAALAREAP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCH
ELPRRILPGVTSYAAAAARTGFSLGEGKERVLILPCPDDMAALRADILSHDVVVLMKVGR
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
RMPDVLALLAELDILEHCALAHRLGLDGEVVLPSLSPAADSPMGRRERPPLDGRLGYLSV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
LLIRRHPPRRFL
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]