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The map label for this gene is emrB [H]

Identifier: 94313238

GI number: 94313238

Start: 906714

End: 908273

Strand: Reverse

Name: emrB [H]

Synonym: Rmet_4313

Alternate gene names: 94313238

Gene position: 908273-906714 (Counterclockwise)

Preceding gene: 94313239

Following gene: 94313237

Centisome position: 35.2

GC content: 59.1

Gene sequence:

>1560_bases
ATGCCAAATCCGATGACAAGCGAATTACCGCCGCCGCTGACCGGTGGCAAGCTGGCAATGGGCAGTCTGGCCGTCGCGCT
GGCCACATTCATGAACGTGCTGGATTCATCCATTGCCAATGTCGCCATCCCGACTATCTCTGGCAACCTCGGCGTCTCCG
TGGATGAAGGCACTTGGGTGATCACGCTGTTCGCGGCCGCCAATGCGGTAGCCATTCCACTGACGGGGTGGCTGACCCAG
CGCGTGGGGCAGATCCGTCTGTTCGTGGGATCGATTCTGCTGTTCGTCCTGTCGTCGTGGCTATGCGGCGTCGCGCCCAA
TCTCACTTTTCTTCTCGCGGCGCGAATCCTTCAGGGAGCCGTCGCGGGTCCGATGATTCCGCTCTCGCAAGCAATTCTCC
TGGGGTCCTATCCCAAGGAGAAAAGCGGTCAGGCACTCGCGTTGTGGGCGATGACCGCTACCGTGGGACCAATAGCCGGC
CCGGCTTTGGGGGGATGGATGACTGACAGCTATTCCTGGTCATGGATCTTCTACATCAACATCCCCGTTGGGCTGTTTGC
TGCCGCAGTCACCTGGGCGATCTATCGTAGCCGCGAGACAACAACCCGTATTCTCCCGATCGACGCCATAGGACTGGCAG
CGCTTGTCACCTGGGTGGCAGCCCTTCAGATCATGCTCGACAAAGGCAAGGATCTCGACTGGTTCGCTTCGCCGGTGATC
ATCAGCCTGGCCATTGTCGCCCTACTCGGCTTCCTGTTCTTCGTGATCTGGGAACTGACCGAAGCCAATCCGATCGTCGA
TTTGAGGCTGTTTAAAGGACGCAACTTCCTGGCCGGAACCATCGCAATATCGGTGGCCTACGCGGTCTTCTTCGCGAATC
TGGTCGTGCTGCCGCAATGGATGCAGCAGTACCTCGCCTATCCAGCCGTCAATGCCGGCCTTGTCACGGCGCCATTGGGC
ATCTTCGCGGTGGTGCTTGCGCCGGTCCTTGGCAAACTGATGCCAAAAGCCGAGTTGCGCATTCTCTCCACCCTTTCGTT
TCTCGGCTTTGCCGCGGTGTTCTTCATGCGCTCCCACTACACGACGGGAGTCGACACATTCACGTTGGTTCTCCCAACGC
TGCTGCAGGGCATTCCAACAGCATTGTTCTTTGTTCCGTTGACGGCCATCATTCTTTCCGGTCTCCCACCAGACAAGATC
CCCGCCGCGGCCGGACTGTCGAACTTTGTCCGCGTGTTCTGCGGCGCTGTGGGAACGTCACTTGCGACCACTGTGTGGAA
CGACCGCGCCATACTGCATCACGCACAACTAGTCGAAAAGGCGAACCCTGGTAATCCGAACTACAGTGCCGCGCTGGAGG
CCATCCAGTCCGTAATGCACCTATCCCCTGGCCAGGCGACCGCCTACTTTGAAAGACAGCTCAATACCCAGGCGATCATG
CTGGGCCTGAACGACATCTTCTGGATATCAGCCGTCATCTTCGTCGCCATTGTTCCACTGATCTGGCTGACCAAGCCAGC
GCGTGGCGCGGCGTCCGGTGCGGCATCCGCTGCCCACTAG

Upstream 100 bases:

>100_bases
CGCGAAACGAAACGACCAGGAATGCGCGGCCGGTGGCCAGTCCTTCGGCACTCGCCAGGGCCTCGTGAAACCGTAGTCGC
CAACGCTAACTGAGGGCATC

Downstream 100 bases:

>100_bases
CTGTCGTCGGTACGATCCTGGGTACATCAGAACAAATCGCAGGTGCTCGCCAAACATACATTTGCTTGCGGATCAGCGTG
TACCTGTATAACCTGACAAC

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MPNPMTSELPPPLTGGKLAMGSLAVALATFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITLFAAANAVAIPLTGWLTQ
RVGQIRLFVGSILLFVLSSWLCGVAPNLTFLLAARILQGAVAGPMIPLSQAILLGSYPKEKSGQALALWAMTATVGPIAG
PALGGWMTDSYSWSWIFYINIPVGLFAAAVTWAIYRSRETTTRILPIDAIGLAALVTWVAALQIMLDKGKDLDWFASPVI
ISLAIVALLGFLFFVIWELTEANPIVDLRLFKGRNFLAGTIAISVAYAVFFANLVVLPQWMQQYLAYPAVNAGLVTAPLG
IFAVVLAPVLGKLMPKAELRILSTLSFLGFAAVFFMRSHYTTGVDTFTLVLPTLLQGIPTALFFVPLTAIILSGLPPDKI
PAAAGLSNFVRVFCGAVGTSLATTVWNDRAILHHAQLVEKANPGNPNYSAALEAIQSVMHLSPGQATAYFERQLNTQAIM
LGLNDIFWISAVIFVAIVPLIWLTKPARGAASGAASAAH

Sequences:

>Translated_519_residues
MPNPMTSELPPPLTGGKLAMGSLAVALATFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITLFAAANAVAIPLTGWLTQ
RVGQIRLFVGSILLFVLSSWLCGVAPNLTFLLAARILQGAVAGPMIPLSQAILLGSYPKEKSGQALALWAMTATVGPIAG
PALGGWMTDSYSWSWIFYINIPVGLFAAAVTWAIYRSRETTTRILPIDAIGLAALVTWVAALQIMLDKGKDLDWFASPVI
ISLAIVALLGFLFFVIWELTEANPIVDLRLFKGRNFLAGTIAISVAYAVFFANLVVLPQWMQQYLAYPAVNAGLVTAPLG
IFAVVLAPVLGKLMPKAELRILSTLSFLGFAAVFFMRSHYTTGVDTFTLVLPTLLQGIPTALFFVPLTAIILSGLPPDKI
PAAAGLSNFVRVFCGAVGTSLATTVWNDRAILHHAQLVEKANPGNPNYSAALEAIQSVMHLSPGQATAYFERQLNTQAIM
LGLNDIFWISAVIFVAIVPLIWLTKPARGAASGAASAAH
>Mature_518_residues
PNPMTSELPPPLTGGKLAMGSLAVALATFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITLFAAANAVAIPLTGWLTQR
VGQIRLFVGSILLFVLSSWLCGVAPNLTFLLAARILQGAVAGPMIPLSQAILLGSYPKEKSGQALALWAMTATVGPIAGP
ALGGWMTDSYSWSWIFYINIPVGLFAAAVTWAIYRSRETTTRILPIDAIGLAALVTWVAALQIMLDKGKDLDWFASPVII
SLAIVALLGFLFFVIWELTEANPIVDLRLFKGRNFLAGTIAISVAYAVFFANLVVLPQWMQQYLAYPAVNAGLVTAPLGI
FAVVLAPVLGKLMPKAELRILSTLSFLGFAAVFFMRSHYTTGVDTFTLVLPTLLQGIPTALFFVPLTAIILSGLPPDKIP
AAAGLSNFVRVFCGAVGTSLATTVWNDRAILHHAQLVEKANPGNPNYSAALEAIQSVMHLSPGQATAYFERQLNTQAIML
GLNDIFWISAVIFVAIVPLIWLTKPARGAASGAASAAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=496, Percent_Identity=49.1935483870968, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1788710, Length=503, Percent_Identity=44.9304174950298, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1790195, Length=383, Percent_Identity=28.4595300261097, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87081983, Length=438, Percent_Identity=24.4292237442922, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=28.4653465346535, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55128; Mature: 54997

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNPMTSELPPPLTGGKLAMGSLAVALATFMNVLDSSIANVAIPTISGNLGVSVDEGTWV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCCEE
ITLFAAANAVAIPLTGWLTQRVGQIRLFVGSILLFVLSSWLCGVAPNLTFLLAARILQGA
EEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VAGPMIPLSQAILLGSYPKEKSGQALALWAMTATVGPIAGPALGGWMTDSYSWSWIFYIN
HCCCCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCEEEEEEEE
IPVGLFAAAVTWAIYRSRETTTRILPIDAIGLAALVTWVAALQIMLDKGKDLDWFASPVI
CCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ISLAIVALLGFLFFVIWELTEANPIVDLRLFKGRNFLAGTIAISVAYAVFFANLVVLPQW
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MQQYLAYPAVNAGLVTAPLGIFAVVLAPVLGKLMPKAELRILSTLSFLGFAAVFFMRSHY
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
TTGVDTFTLVLPTLLQGIPTALFFVPLTAIILSGLPPDKIPAAAGLSNFVRVFCGAVGTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LATTVWNDRAILHHAQLVEKANPGNPNYSAALEAIQSVMHLSPGQATAYFERQLNTQAIM
HHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEE
LGLNDIFWISAVIFVAIVPLIWLTKPARGAASGAASAAH
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PNPMTSELPPPLTGGKLAMGSLAVALATFMNVLDSSIANVAIPTISGNLGVSVDEGTWV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCCEE
ITLFAAANAVAIPLTGWLTQRVGQIRLFVGSILLFVLSSWLCGVAPNLTFLLAARILQGA
EEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VAGPMIPLSQAILLGSYPKEKSGQALALWAMTATVGPIAGPALGGWMTDSYSWSWIFYIN
HCCCCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCEEEEEEEE
IPVGLFAAAVTWAIYRSRETTTRILPIDAIGLAALVTWVAALQIMLDKGKDLDWFASPVI
CCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ISLAIVALLGFLFFVIWELTEANPIVDLRLFKGRNFLAGTIAISVAYAVFFANLVVLPQW
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MQQYLAYPAVNAGLVTAPLGIFAVVLAPVLGKLMPKAELRILSTLSFLGFAAVFFMRSHY
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
TTGVDTFTLVLPTLLQGIPTALFFVPLTAIILSGLPPDKIPAAAGLSNFVRVFCGAVGTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LATTVWNDRAILHHAQLVEKANPGNPNYSAALEAIQSVMHLSPGQATAYFERQLNTQAIM
HHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEE
LGLNDIFWISAVIFVAIVPLIWLTKPARGAASGAASAAH
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]