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The map label for this gene is emrA [H]

Identifier: 94313239

GI number: 94313239

Start: 908306

End: 909577

Strand: Reverse

Name: emrA [H]

Synonym: Rmet_4314

Alternate gene names: 94313239

Gene position: 909577-908306 (Counterclockwise)

Preceding gene: 94313240

Following gene: 94313238

Centisome position: 35.25

GC content: 63.6

Gene sequence:

>1272_bases
ATGACCACGACCAACCCGAACCCCACTGACAATCCCAAGGCCGCGTCCGGCGCGCCGAGAACCCGCAAGCGTCTGCTGGC
CCTGCTTGGGCTGGGCGCTCTGGTGGCAGCGGCAGGCTACGGCGCGTACTACTACAGCGTGGCACGCTTTCACGAAGACA
CCGATGACGCCTACGTCAACGGCAATCTGGTGCAACTCACCCCTCAGGTGAGCGGGACGGTTGTCGCAGTCAATGCCGAC
GACACGGAGATCGTCAGGGCGGGCGATGCGATCGTCACGCTTGATCCCGCAGACGCTCGCATCGCTCTGGCCAACGCCGA
GGCGGCGCTCGGGCAGACCGTTCGCCAGGTAAGTGCGTTATATGTCAACAACAACACGCTGGCTGCAACTGTTCAACAGC
GGCAGGTGGACCTTTCCCGGGCGCGAGATGATCTGCGGCGTCGAACCGAAGCCTCGGAAAGTGGCGCGGTTGCCGCGGAG
GACGTCGCGCACGCAAGGGACACCGTGAAGGCTGCCGAAGCCGCGCTGGAAACGGCGAAGCAACAGCTCGCCTCGAACCA
TGCGTTGACCGACCAGGCTAGCGTGGCCGATCACCCCAGTGTTCAAGCAGCCGCCACACGCGTGCGGGATGCCTATCTCG
CCAATGCGCGAAACACGCTGCCCGCTCCCGTGACCGGCTATGTCGCCCGTCGATCCGTGCAAGTCGGTCAACGTGTCGCG
CCTGGCAATCCACTCATGGCCATCGTGCCTCTCGATGGAGTCTGGGTAGATGCCAACTTCAAGGAGGTTCAGCTCAAGCA
CATTCGAATTGGCCAGCCAGTAGAACTCCACGCCGATCTGTACGGCGGCAAGGTGACCTATCACGGCAAGGTCGTCGGCT
TCTCCGCGGGCACCGGTAGCGCGTTCTCCGCGCTACCAGCGCAGAACGCGACTGGCAACTGGATAAAGGTCGTCCAGCGA
CTGCCCGTGCGGATCGCACTGGATCCGGTGGAACTGCGCGCGCACCCACTGCAGGTTGGCCTGTCTATGCAGGTCGATGT
GGAGACCAGGGAGCGCGGCGGAGAGCTTTCCCCCGCCAAGGGCACAAGCACACCAATTCGCACCGCCTATCGCACCGATG
TGTTCGAACGCTACGGTGCGGAAGCCGACAGCGAGATCGATCGGATCATCGCCCGGAACAGCAATAGCAACAGCGACAAC
TCCACGCGAAACGAAACGACCAGGAATGCGCGGCCGGTGGCCAGTCCTTCGGCACTCGCCAGGGCCTCGTGA

Upstream 100 bases:

>100_bases
TTGCGCTGGCAGCGGCCCTCGGCGGCGGGTACACGGCCGACGACACACAAAGCGCGGACACAACCCGGTGAGCCGCCACG
CCACAGATACAGGTGACATG

Downstream 100 bases:

>100_bases
AACCGTAGTCGCCAACGCTAACTGAGGGCATCATGCCAAATCCGATGACAAGCGAATTACCGCCGCCGCTGACCGGTGGC
AAGCTGGCAATGGGCAGTCT

Product: MFS efflux pump, membrane fusion protein, EmrA subfamily

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTTTNPNPTDNPKAASGAPRTRKRLLALLGLGALVAAAGYGAYYYSVARFHEDTDDAYVNGNLVQLTPQVSGTVVAVNAD
DTEIVRAGDAIVTLDPADARIALANAEAALGQTVRQVSALYVNNNTLAATVQQRQVDLSRARDDLRRRTEASESGAVAAE
DVAHARDTVKAAEAALETAKQQLASNHALTDQASVADHPSVQAAATRVRDAYLANARNTLPAPVTGYVARRSVQVGQRVA
PGNPLMAIVPLDGVWVDANFKEVQLKHIRIGQPVELHADLYGGKVTYHGKVVGFSAGTGSAFSALPAQNATGNWIKVVQR
LPVRIALDPVELRAHPLQVGLSMQVDVETRERGGELSPAKGTSTPIRTAYRTDVFERYGAEADSEIDRIIARNSNSNSDN
STRNETTRNARPVASPSALARAS

Sequences:

>Translated_423_residues
MTTTNPNPTDNPKAASGAPRTRKRLLALLGLGALVAAAGYGAYYYSVARFHEDTDDAYVNGNLVQLTPQVSGTVVAVNAD
DTEIVRAGDAIVTLDPADARIALANAEAALGQTVRQVSALYVNNNTLAATVQQRQVDLSRARDDLRRRTEASESGAVAAE
DVAHARDTVKAAEAALETAKQQLASNHALTDQASVADHPSVQAAATRVRDAYLANARNTLPAPVTGYVARRSVQVGQRVA
PGNPLMAIVPLDGVWVDANFKEVQLKHIRIGQPVELHADLYGGKVTYHGKVVGFSAGTGSAFSALPAQNATGNWIKVVQR
LPVRIALDPVELRAHPLQVGLSMQVDVETRERGGELSPAKGTSTPIRTAYRTDVFERYGAEADSEIDRIIARNSNSNSDN
STRNETTRNARPVASPSALARAS
>Mature_422_residues
TTTNPNPTDNPKAASGAPRTRKRLLALLGLGALVAAAGYGAYYYSVARFHEDTDDAYVNGNLVQLTPQVSGTVVAVNADD
TEIVRAGDAIVTLDPADARIALANAEAALGQTVRQVSALYVNNNTLAATVQQRQVDLSRARDDLRRRTEASESGAVAAED
VAHARDTVKAAEAALETAKQQLASNHALTDQASVADHPSVQAAATRVRDAYLANARNTLPAPVTGYVARRSVQVGQRVAP
GNPLMAIVPLDGVWVDANFKEVQLKHIRIGQPVELHADLYGGKVTYHGKVVGFSAGTGSAFSALPAQNATGNWIKVVQRL
PVRIALDPVELRAHPLQVGLSMQVDVETRERGGELSPAKGTSTPIRTAYRTDVFERYGAEADSEIDRIIARNSNSNSDNS
TRNETTRNARPVASPSALARAS

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=358, Percent_Identity=48.8826815642458, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1788711, Length=372, Percent_Identity=41.9354838709677, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1790519, Length=291, Percent_Identity=32.9896907216495, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI87081951, Length=299, Percent_Identity=29.0969899665552, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789637, Length=315, Percent_Identity=24.1269841269841, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44921; Mature: 44789

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTNPNPTDNPKAASGAPRTRKRLLALLGLGALVAAAGYGAYYYSVARFHEDTDDAYVN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEC
GNLVQLTPQVSGTVVAVNADDTEIVRAGDAIVTLDPADARIALANAEAALGQTVRQVSAL
CCEEEECCCCCCEEEEEECCCCEEEECCCEEEEECCCCCEEEEECCHHHHHHHHHHHHEE
YVNNNTLAATVQQRQVDLSRARDDLRRRTEASESGAVAAEDVAHARDTVKAAEAALETAK
EECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
QQLASNHALTDQASVADHPSVQAAATRVRDAYLANARNTLPAPVTGYVARRSVQVGQRVA
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
PGNPLMAIVPLDGVWVDANFKEVQLKHIRIGQPVELHADLYGGKVTYHGKVVGFSAGTGS
CCCCEEEEEECCCEEECCCHHEEEEEEEECCCCEEEEEEECCCEEEEEEEEEEEECCCCC
AFSALPAQNATGNWIKVVQRLPVRIALDPVELRAHPLQVGLSMQVDVETRERGGELSPAK
CHHCCCCCCCCCHHHHHHHHCCCEEEECHHHHCCCEEEECCEEEEEEHHHHCCCCCCCCC
GTSTPIRTAYRTDVFERYGAEADSEIDRIIARNSNSNSDNSTRNETTRNARPVASPSALA
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RAS
CCC
>Mature Secondary Structure 
TTTNPNPTDNPKAASGAPRTRKRLLALLGLGALVAAAGYGAYYYSVARFHEDTDDAYVN
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEC
GNLVQLTPQVSGTVVAVNADDTEIVRAGDAIVTLDPADARIALANAEAALGQTVRQVSAL
CCEEEECCCCCCEEEEEECCCCEEEECCCEEEEECCCCCEEEEECCHHHHHHHHHHHHEE
YVNNNTLAATVQQRQVDLSRARDDLRRRTEASESGAVAAEDVAHARDTVKAAEAALETAK
EECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
QQLASNHALTDQASVADHPSVQAAATRVRDAYLANARNTLPAPVTGYVARRSVQVGQRVA
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
PGNPLMAIVPLDGVWVDANFKEVQLKHIRIGQPVELHADLYGGKVTYHGKVVGFSAGTGS
CCCCEEEEEECCCEEECCCHHEEEEEEEECCCCEEEEEEECCCEEEEEEEEEEEECCCCC
AFSALPAQNATGNWIKVVQRLPVRIALDPVELRAHPLQVGLSMQVDVETRERGGELSPAK
CHHCCCCCCCCCHHHHHHHHCCCEEEECHHHHCCCEEEECCEEEEEEHHHHCCCCCCCCC
GTSTPIRTAYRTDVFERYGAEADSEIDRIIARNSNSNSDNSTRNETTRNARPVASPSALA
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RAS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]