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The map label for this gene is citE [H]

Identifier: 94312765

GI number: 94312765

Start: 376796

End: 377626

Strand: Direct

Name: citE [H]

Synonym: Rmet_3835

Alternate gene names: 94312765

Gene position: 376796-377626 (Clockwise)

Preceding gene: 94312764

Following gene: 94312766

Centisome position: 14.6

GC content: 68.59

Gene sequence:

>831_bases
ATGACAACCACCCGAGTGCCGCGCAGTTATCTGTTCGTTCCGGCATCGCGTCCGGAGCGTATTGGCAAGGCGATCGCGGC
TGGCGCCGACGCCGTGATCGTCGATCTTGAGGATGCGGTGGCGCCCGATGCCAAGGCGGCTGCGCGCGCTGGCCTGGCCG
AGCCCTGGCGCGACTTGCAGGCCCAGGCGGATGCCGCCGGCCTAGCATTGCTGCTCCGCATCAATGGTGCTGACACCTCG
TACTTTGCCGAAGACGTGGGCTTTTGCCGCACAAGCGGCGTTCGCGAGATCGTGCTGCCCAAGGCCGACAAGGCGGCGCT
GGCCGCGCTGCGTGCCGAACTGCCCGAGACGCCGTGCGGCGCCTTGTTGGAGAGCGCGGCCGGGTTTGCCGATCTGGCCC
ATGTGGCGCGCGCGCCAGGCGTATCGCGACTGCTGTTCGGCAGCATCGACCTGATGTTCGACCTTGACGTGGGTGACGAC
GACGCGCCGCTCCACCATTTCCGTAGCGAATTGGTCATGCATTCCCGGGCGGCCGGACTGCCGGCGCCGGTCGACGGTGT
CTGCACGGCCATCGGCGATGCCGACGCGCTGGCCGCCGACACCGCGCGGGCACGGCGTTTCGGGTTTGGGGCCAAATTGC
TGATTCATCCGAATCAGGTGGGGGGTGTCCATGCCGCCCTGGCGCCATCGGCCGACGAACTGGCCTGGGCGCGTCGCGTG
GTCGAGCAGGCCGCCGCCGCGCAGGGTGCCGCGATTGCCGTCGACGGGAAGATGGTTGATCGCCCAGTGCTTGAACGCGC
ACAACGTATCGTCGCGGCGGGTGCCGCCTGA

Upstream 100 bases:

>100_bases
GGTGGGCGAGCACACGGACGCCATCCTTGCCGAGGCCGGTTGCACTGCCGAACAGATCGCCCGGCTTCATGCCGACGGCG
TGGTCTGACGGAGGCGGGCG

Downstream 100 bases:

>100_bases
CGACGCTTCCGGCCCTCCGAATTGGCGCTGATGGGGCGCCAATGGAGCGCCAATGCGTGTTTTCCCTGAGCGTGGGATTA
CTGCAAAACCTGCCGGTTCG

Product: putative aldolase/citrate lyase

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTTTRVPRSYLFVPASRPERIGKAIAAGADAVIVDLEDAVAPDAKAAARAGLAEPWRDLQAQADAAGLALLLRINGADTS
YFAEDVGFCRTSGVREIVLPKADKAALAALRAELPETPCGALLESAAGFADLAHVARAPGVSRLLFGSIDLMFDLDVGDD
DAPLHHFRSELVMHSRAAGLPAPVDGVCTAIGDADALAADTARARRFGFGAKLLIHPNQVGGVHAALAPSADELAWARRV
VEQAAAAQGAAIAVDGKMVDRPVLERAQRIVAAGAA

Sequences:

>Translated_276_residues
MTTTRVPRSYLFVPASRPERIGKAIAAGADAVIVDLEDAVAPDAKAAARAGLAEPWRDLQAQADAAGLALLLRINGADTS
YFAEDVGFCRTSGVREIVLPKADKAALAALRAELPETPCGALLESAAGFADLAHVARAPGVSRLLFGSIDLMFDLDVGDD
DAPLHHFRSELVMHSRAAGLPAPVDGVCTAIGDADALAADTARARRFGFGAKLLIHPNQVGGVHAALAPSADELAWARRV
VEQAAAAQGAAIAVDGKMVDRPVLERAQRIVAAGAA
>Mature_275_residues
TTTRVPRSYLFVPASRPERIGKAIAAGADAVIVDLEDAVAPDAKAAARAGLAEPWRDLQAQADAAGLALLLRINGADTSY
FAEDVGFCRTSGVREIVLPKADKAALAALRAELPETPCGALLESAAGFADLAHVARAPGVSRLLFGSIDLMFDLDVGDDD
APLHHFRSELVMHSRAAGLPAPVDGVCTAIGDADALAADTARARRFGFGAKLLIHPNQVGGVHAALAPSADELAWARRVV
EQAAAAQGAAIAVDGKMVDRPVLERAQRIVAAGAA

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=296, Percent_Identity=26.3513513513513, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI87081764, Length=294, Percent_Identity=29.9319727891156, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17557458, Length=299, Percent_Identity=27.7591973244147, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 28519; Mature: 28387

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTRVPRSYLFVPASRPERIGKAIAAGADAVIVDLEDAVAPDAKAAARAGLAEPWRDLQ
CCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEECHHHCCCCHHHHHHCCCHHHHHHHH
AQADAAGLALLLRINGADTSYFAEDVGFCRTSGVREIVLPKADKAALAALRAELPETPCG
HHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCHH
ALLESAAGFADLAHVARAPGVSRLLFGSIDLMFDLDVGDDDAPLHHFRSELVMHSRAAGL
HHHHHHCCHHHHHHHHHCCCHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
PAPVDGVCTAIGDADALAADTARARRFGFGAKLLIHPNQVGGVHAALAPSADELAWARRV
CCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCHHHHHHHHHH
VEQAAAAQGAAIAVDGKMVDRPVLERAQRIVAAGAA
HHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTTRVPRSYLFVPASRPERIGKAIAAGADAVIVDLEDAVAPDAKAAARAGLAEPWRDLQ
CCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEECHHHCCCCHHHHHHCCCHHHHHHHH
AQADAAGLALLLRINGADTSYFAEDVGFCRTSGVREIVLPKADKAALAALRAELPETPCG
HHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCHH
ALLESAAGFADLAHVARAPGVSRLLFGSIDLMFDLDVGDDDAPLHHFRSELVMHSRAAGL
HHHHHHCCHHHHHHHHHCCCHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
PAPVDGVCTAIGDADALAADTARARRFGFGAKLLIHPNQVGGVHAALAPSADELAWARRV
CCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCHHHHHHHHHH
VEQAAAAQGAAIAVDGKMVDRPVLERAQRIVAAGAA
HHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9457870 [H]