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The map label for this gene is tmk

Identifier: 94310766

GI number: 94310766

Start: 1983942

End: 1984544

Strand: Reverse

Name: tmk

Synonym: Rmet_1828

Alternate gene names: 94310766

Gene position: 1984544-1983942 (Counterclockwise)

Preceding gene: 94310767

Following gene: 94310765

Centisome position: 50.52

GC content: 65.51

Gene sequence:

>603_bases
ATGCGCGGAAAATTCATCACCTTCGAGGGCATCGATGGCGCGGGCAAGAGCACCCATATCGAGTGGGTGGCCGAGCGCCT
GCGCGCGCGCGCAAATGTCGTCACTACCCGGGAGCCGGGCGGCACGCCGCTGGGCGAAGACCTGCGCCAGTTGCTGCTGC
ACCGGAAGATGCATCTTGAAACCGAGGCGCTGCTGATGTTCGCGGCGCGCCGCGAGCACATCGCCGAAGTCATCGAGCCC
GCGCTGGCGCGCGGCGACTGGGTCATCTCGGATCGTTTCACCGATGCCACGTTTGCCTATCAGGGCGGTGGCCGCGGGCT
GGCGCTCGATCGGCTGGCCACGCTCGAGCAGTGGGTGCAGAGCGGTCTTCAGCCCGACCTGACGCTGCTGTTTGACGTTC
CGCTGGAAACCGCGAGCGCACGTCTGGCCGGGGCCCGTGAGCCCGACAAGTTTGAGGCCGAGTCCCGCGCCTTCTTCGAA
CGCACCCGCACTGAGTATCTGCGCCGTGCGGCGGAAGCGCCACAGCGCTTTCGGGTCATCGACGCAACGCGCTCGATCGA
CGAGATTCGTGTGGCGCTTGAAGAAATTATTGCAACGCTTTGA

Upstream 100 bases:

>100_bases
TATTTCGTGGCACGCGGCGACGGCAGCAGCCATTTCTCCAATTCACTACCCGAACACAATCGCGCGGTCGACAAGTACCA
GCGCGGCAAACCATAAGGTC

Downstream 100 bases:

>100_bases
TTTTTAATGGCTATATCCATGTGGTGATGTCGATGTCATTGACTCCGACAATACATGAGATGGCTGCGCTAACTGATTGA
ACCTGAATGCTTTATCCCTG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MRGKFITFEGIDGAGKSTHIEWVAERLRARANVVTTREPGGTPLGEDLRQLLLHRKMHLETEALLMFAARREHIAEVIEP
ALARGDWVISDRFTDATFAYQGGGRGLALDRLATLEQWVQSGLQPDLTLLFDVPLETASARLAGAREPDKFEAESRAFFE
RTRTEYLRRAAEAPQRFRVIDATRSIDEIRVALEEIIATL

Sequences:

>Translated_200_residues
MRGKFITFEGIDGAGKSTHIEWVAERLRARANVVTTREPGGTPLGEDLRQLLLHRKMHLETEALLMFAARREHIAEVIEP
ALARGDWVISDRFTDATFAYQGGGRGLALDRLATLEQWVQSGLQPDLTLLFDVPLETASARLAGAREPDKFEAESRAFFE
RTRTEYLRRAAEAPQRFRVIDATRSIDEIRVALEEIIATL
>Mature_200_residues
MRGKFITFEGIDGAGKSTHIEWVAERLRARANVVTTREPGGTPLGEDLRQLLLHRKMHLETEALLMFAARREHIAEVIEP
ALARGDWVISDRFTDATFAYQGGGRGLALDRLATLEQWVQSGLQPDLTLLFDVPLETASARLAGAREPDKFEAESRAFFE
RTRTEYLRRAAEAPQRFRVIDATRSIDEIRVALEEIIATL

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=201, Percent_Identity=43.7810945273632, Blast_Score=138, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_RALME (Q1LMB9)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583976.1
- ProteinModelPortal:   Q1LMB9
- SMR:   Q1LMB9
- STRING:   Q1LMB9
- GeneID:   4038630
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1828
- eggNOG:   COG0125
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- PhylomeDB:   Q1LMB9
- ProtClustDB:   PRK00698
- BioCyc:   RMET266264:RMET_1828-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 22544; Mature: 22544

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGKFITFEGIDGAGKSTHIEWVAERLRARANVVTTREPGGTPLGEDLRQLLLHRKMHLE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHH
TEALLMFAARREHIAEVIEPALARGDWVISDRFTDATFAYQGGGRGLALDRLATLEQWVQ
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHH
SGLQPDLTLLFDVPLETASARLAGAREPDKFEAESRAFFERTRTEYLRRAAEAPQRFRVI
CCCCCCEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHH
DATRSIDEIRVALEEIIATL
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRGKFITFEGIDGAGKSTHIEWVAERLRARANVVTTREPGGTPLGEDLRQLLLHRKMHLE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHH
TEALLMFAARREHIAEVIEPALARGDWVISDRFTDATFAYQGGGRGLALDRLATLEQWVQ
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHH
SGLQPDLTLLFDVPLETASARLAGAREPDKFEAESRAFFERTRTEYLRRAAEAPQRFRVI
CCCCCCEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHH
DATRSIDEIRVALEEIIATL
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA