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The map label for this gene is holB [H]

Identifier: 94310765

GI number: 94310765

Start: 1982842

End: 1983855

Strand: Reverse

Name: holB [H]

Synonym: Rmet_1827

Alternate gene names: 94310765

Gene position: 1983855-1982842 (Counterclockwise)

Preceding gene: 94310766

Following gene: 94310764

Centisome position: 50.5

GC content: 67.65

Gene sequence:

>1014_bases
ATGCTTTATCCCTGGCAACGAGATGACTGGCATCGGCTGACCGCGCTACGCGACCGGCTGCCGCACGCGCTGCTGCTTCA
CGGCCAGCAAGGCATCGGCAAGCGCGATCTGGCGTTGCACTTCGCGCAGGGCCTGCTTTGCGAAAGCGCGCTGCCCGATG
GCCAGCCCTGCAATACCTGCAGCGCCTGCCACTGGTTTGGTCAGGGCAACCATCCCGATTTCACGGTGGTTCGGCCCGAG
GCACTGGAAGCCGGCGCTGGAGAGGCCGAAGGCGACGGCGAGAGCGGCAGCAAGAAGAAGGCGCCGAGCAAGATCATCCG
CATGGAGCAGGTGCGGGCGCTGATCGAAGCGGTGGGTGTTGGCACGCACCGCGCGGGTTTGCGCGTGGTGGTGGTCTATC
CGCTCGACGCGCTGCAGACTGAAGGCGCCAACGCGCTGCTCAAGACGCTCGAGGAGCCGCCGCCGTCCACGGTGTTCCTG
CTGGTCACGGATCGTATCGACCGTGTGTTACCGACGATCCTGTCCCGCTGCCGCCAATTCTCGATGACGCGGCCGACCAG
CGCGGATGCGCTCGATTGGCTGCGCGGACAGGGGGTGGCCGATGTGGAGGCGCAACTGGCGCTGGCTGGGGGGGCGCCAC
TGACGGCGCTGCACGCGGCCGAGGCCGAGGAACAGCCCTTGCAGCGCTGGCTGGTCGGCCAGCTCGGTAGCGCGGCTGCG
CTGGATGCCCTGGCCGCCGCGGAGCAATTGCAGAAGCTGCCGGTTCCCGCCGTGCTCGGCACCCTGCAACGCTGGACGTA
CGATCTGCTGGCGCTCTGCCTGGGTACGGGTGCCGCACGTTACTTCCCGAAGGAGCAGACGGCACTGGCCCGCTGCGCCA
GCGCAACCGACGCGCATCGGCTGCAGGCGTTCGCCGCGCGGCTGGTCGGACATCGCCGCAATGAGAACCATCCGTTGGCG
GCGCGCCTCGTGATGGAGTCGGTGTTGCTCGATTACCGACAATTGTTCCGTTAG

Upstream 100 bases:

>100_bases
TTGCAACGCTTTGATTTTTAATGGCTATATCCATGTGGTGATGTCGATGTCATTGACTCCGACAATACATGAGATGGCTG
CGCTAACTGATTGAACCTGA

Downstream 100 bases:

>100_bases
CGTGCGTCTCGCGGTAGGAATCGTGCCGCACACGTGGTACAAGTCGAACAAAGCGACTTTCAAATAGCCCGCACAAAGCG
GGCAACGCTGCAACAAGGGG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MLYPWQRDDWHRLTALRDRLPHALLLHGQQGIGKRDLALHFAQGLLCESALPDGQPCNTCSACHWFGQGNHPDFTVVRPE
ALEAGAGEAEGDGESGSKKKAPSKIIRMEQVRALIEAVGVGTHRAGLRVVVVYPLDALQTEGANALLKTLEEPPPSTVFL
LVTDRIDRVLPTILSRCRQFSMTRPTSADALDWLRGQGVADVEAQLALAGGAPLTALHAAEAEEQPLQRWLVGQLGSAAA
LDALAAAEQLQKLPVPAVLGTLQRWTYDLLALCLGTGAARYFPKEQTALARCASATDAHRLQAFAARLVGHRRNENHPLA
ARLVMESVLLDYRQLFR

Sequences:

>Translated_337_residues
MLYPWQRDDWHRLTALRDRLPHALLLHGQQGIGKRDLALHFAQGLLCESALPDGQPCNTCSACHWFGQGNHPDFTVVRPE
ALEAGAGEAEGDGESGSKKKAPSKIIRMEQVRALIEAVGVGTHRAGLRVVVVYPLDALQTEGANALLKTLEEPPPSTVFL
LVTDRIDRVLPTILSRCRQFSMTRPTSADALDWLRGQGVADVEAQLALAGGAPLTALHAAEAEEQPLQRWLVGQLGSAAA
LDALAAAEQLQKLPVPAVLGTLQRWTYDLLALCLGTGAARYFPKEQTALARCASATDAHRLQAFAARLVGHRRNENHPLA
ARLVMESVLLDYRQLFR
>Mature_337_residues
MLYPWQRDDWHRLTALRDRLPHALLLHGQQGIGKRDLALHFAQGLLCESALPDGQPCNTCSACHWFGQGNHPDFTVVRPE
ALEAGAGEAEGDGESGSKKKAPSKIIRMEQVRALIEAVGVGTHRAGLRVVVVYPLDALQTEGANALLKTLEEPPPSTVFL
LVTDRIDRVLPTILSRCRQFSMTRPTSADALDWLRGQGVADVEAQLALAGGAPLTALHAAEAEEQPLQRWLVGQLGSAAA
LDALAAAEQLQKLPVPAVLGTLQRWTYDLLALCLGTGAARYFPKEQTALARCASATDAHRLQAFAARLVGHRRNENHPLA
ARLVMESVLLDYRQLFR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=177, Percent_Identity=33.8983050847458, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787341, Length=195, Percent_Identity=33.8461538461538, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36677; Mature: 36677

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPWQRDDWHRLTALRDRLPHALLLHGQQGIGKRDLALHFAQGLLCESALPDGQPCNTC
CCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
SACHWFGQGNHPDFTVVRPEALEAGAGEAEGDGESGSKKKAPSKIIRMEQVRALIEAVGV
HHHHHCCCCCCCCEEEECHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GTHRAGLRVVVVYPLDALQTEGANALLKTLEEPPPSTVFLLVTDRIDRVLPTILSRCRQF
CCCCCCCEEEEEECCHHHHCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
SMTRPTSADALDWLRGQGVADVEAQLALAGGAPLTALHAAEAEEQPLQRWLVGQLGSAAA
CCCCCCCHHHHHHHHCCCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
LDALAAAEQLQKLPVPAVLGTLQRWTYDLLALCLGTGAARYFPKEQTALARCASATDAHR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCCHHHHH
LQAFAARLVGHRRNENHPLAARLVMESVLLDYRQLFR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLYPWQRDDWHRLTALRDRLPHALLLHGQQGIGKRDLALHFAQGLLCESALPDGQPCNTC
CCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
SACHWFGQGNHPDFTVVRPEALEAGAGEAEGDGESGSKKKAPSKIIRMEQVRALIEAVGV
HHHHHCCCCCCCCEEEECHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GTHRAGLRVVVVYPLDALQTEGANALLKTLEEPPPSTVFLLVTDRIDRVLPTILSRCRQF
CCCCCCCEEEEEECCHHHHCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
SMTRPTSADALDWLRGQGVADVEAQLALAGGAPLTALHAAEAEEQPLQRWLVGQLGSAAA
CCCCCCCHHHHHHHHCCCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
LDALAAAEQLQKLPVPAVLGTLQRWTYDLLALCLGTGAARYFPKEQTALARCASATDAHR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCCHHHHH
LQAFAARLVGHRRNENHPLAARLVMESVLLDYRQLFR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]