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The map label for this gene is comM [H]

Identifier: 93007271

GI number: 93007271

Start: 3021084

End: 3022634

Strand: Reverse

Name: comM [H]

Synonym: Pcryo_2447

Alternate gene names: 93007271

Gene position: 3022634-3021084 (Counterclockwise)

Preceding gene: 93007274

Following gene: 93007267

Centisome position: 98.78

GC content: 48.74

Gene sequence:

>1551_bases
ATGTCGTTTGCTCAAGTCTATACCCGCTCGGTCGTCGGGCTGCACGCACCTGAGGTCATCATTGAAGTGCATTTGTCGCA
AGGACTGCCAGCATTGACCATTGTTGGTTTGCCTGAAGCGGCGGTACGTGAGAGCAAGGATAGAGTGCGCTCAGCGATAC
TCAATTCTGGATTTCAGTTTCCTAATAGACGTTTGACGATTAATTTAGCACCCGCCGATTTACCAAAGGATGGTGCTCGC
CTTGATTTACCGATTGCTATCGGTATTTTGGCAGCCAGTGATCAGTTGGATCCAACGGTATTGTCAGGGTTTGAGTTTAT
TGGGGAGTTGGCGCTCAATGGTGAGCTGCGGCAAGTAAGCGGTAGTTTGGCGGTTGCACGAGCAATGAAAGCTGAGGTAT
TGGCGACGCAACTGTTAAAAGGTGATGAGAAAATGATGTCGTCAACACGGCAACTCATTGTCCCAACCATCAATGGGGCA
GAAGCTAGCCGTGTGGATGGCATCACCGTATTGGCAGCGACAAATTTAAAAGCCGTATGCAATCACTTGCAGTCACTTAC
CGATAGCAGTCTTTCGGATGAGCGCTTAGCGGCAGTAGAGCCAAATCAAATCCAGCAGCATATAGGCTATCAAGTAGATT
TGGCAGACGTAAAAGGACAGCATCATGCACGGCGTGCCTTAGAGATTGCAGCAGCAGGTGGTCATTCATTGTTACTTACA
GGACCACCGGGTTCAGGTAAGACCTTGATGGCGTCACGCCTGCCAACTATTTTGCCAGATTTGAGTGCAGAAGATGCGCT
AGAGGTTGCCAGTACGTATTCGGTTGCCGATAGTGATTATGATTATGGCACACGACCTTTTAGACAAGTTCATCACACCA
TATCAGCGGTGGCATTGGTCGGTGGCGGTTCACGTCCCAAACCGGGTGAGATTACCCTTGCCAATAAGGGCGTACTATTC
CTTGATGAGTTGCCTGAATTTGATCGTACGGTGTTAGAGGTACTGCGCCAACCACTAGAGGCAAAACAAATCACTATTAG
CCGTGCCAACTCACAGATGACCTTTCCAGCAAATTTTCAACTGGTGGCGGCGATGAACCCGTGCCCGTGTGGCTATGATG
GTGATGGCTCAGGGCGGTGTCGTTGCAGAGCTGAGCAGATTAAACGCTATCAAGATAAATTATCAGGACCACTACTTGAT
CGTATTGATTTACATATTACCGTACCAGCATTACCGATTGCGGATTTGCAAAACGCTCAAGCGGGAGAAACCTCCGAGCA
AGTACGTACTCGAGTATCGGCAGCACATAATCGCCAATTAATACGACAGAACAAGGTCAATAATGAGCTGAGTCCTAGTG
AAATTGATAAATATATTCAGCTAGGGGCGGGCGAGCAGCAGCTATTACAGCTCGCCCAGCAGCGCCTTAATTTATCTGCT
CGTGGCTACCATAGGGTACTACGCGTTGCACGAACTATTGCTGATTTGGCGGACAGCGCGGATATCACGAGCGCCCATGT
ATCTGAAGCGCTCAGTTATCGTAGTAAATGA

Upstream 100 bases:

>100_bases
ACGTCTACGCTAGTGAATAAACCAAACAAATAAATACGCAACATTTGCGCACTTATTAATAATCCTCTATGATCGATCAA
TCTCTCTATAGGAAAGGCTT

Downstream 100 bases:

>100_bases
TAGTTTTAAGATCCAATTATAGTAGATACACTTTTAAAAGAATACAGTGCTACTGGGATTAATTATTCGCAGCCTCTGTC
TGCGTAGGCACAGTAAGCAA

Product: Mg chelatase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MSFAQVYTRSVVGLHAPEVIIEVHLSQGLPALTIVGLPEAAVRESKDRVRSAILNSGFQFPNRRLTINLAPADLPKDGAR
LDLPIAIGILAASDQLDPTVLSGFEFIGELALNGELRQVSGSLAVARAMKAEVLATQLLKGDEKMMSSTRQLIVPTINGA
EASRVDGITVLAATNLKAVCNHLQSLTDSSLSDERLAAVEPNQIQQHIGYQVDLADVKGQHHARRALEIAAAGGHSLLLT
GPPGSGKTLMASRLPTILPDLSAEDALEVASTYSVADSDYDYGTRPFRQVHHTISAVALVGGGSRPKPGEITLANKGVLF
LDELPEFDRTVLEVLRQPLEAKQITISRANSQMTFPANFQLVAAMNPCPCGYDGDGSGRCRCRAEQIKRYQDKLSGPLLD
RIDLHITVPALPIADLQNAQAGETSEQVRTRVSAAHNRQLIRQNKVNNELSPSEIDKYIQLGAGEQQLLQLAQQRLNLSA
RGYHRVLRVARTIADLADSADITSAHVSEALSYRSK

Sequences:

>Translated_516_residues
MSFAQVYTRSVVGLHAPEVIIEVHLSQGLPALTIVGLPEAAVRESKDRVRSAILNSGFQFPNRRLTINLAPADLPKDGAR
LDLPIAIGILAASDQLDPTVLSGFEFIGELALNGELRQVSGSLAVARAMKAEVLATQLLKGDEKMMSSTRQLIVPTINGA
EASRVDGITVLAATNLKAVCNHLQSLTDSSLSDERLAAVEPNQIQQHIGYQVDLADVKGQHHARRALEIAAAGGHSLLLT
GPPGSGKTLMASRLPTILPDLSAEDALEVASTYSVADSDYDYGTRPFRQVHHTISAVALVGGGSRPKPGEITLANKGVLF
LDELPEFDRTVLEVLRQPLEAKQITISRANSQMTFPANFQLVAAMNPCPCGYDGDGSGRCRCRAEQIKRYQDKLSGPLLD
RIDLHITVPALPIADLQNAQAGETSEQVRTRVSAAHNRQLIRQNKVNNELSPSEIDKYIQLGAGEQQLLQLAQQRLNLSA
RGYHRVLRVARTIADLADSADITSAHVSEALSYRSK
>Mature_515_residues
SFAQVYTRSVVGLHAPEVIIEVHLSQGLPALTIVGLPEAAVRESKDRVRSAILNSGFQFPNRRLTINLAPADLPKDGARL
DLPIAIGILAASDQLDPTVLSGFEFIGELALNGELRQVSGSLAVARAMKAEVLATQLLKGDEKMMSSTRQLIVPTINGAE
ASRVDGITVLAATNLKAVCNHLQSLTDSSLSDERLAAVEPNQIQQHIGYQVDLADVKGQHHARRALEIAAAGGHSLLLTG
PPGSGKTLMASRLPTILPDLSAEDALEVASTYSVADSDYDYGTRPFRQVHHTISAVALVGGGSRPKPGEITLANKGVLFL
DELPEFDRTVLEVLRQPLEAKQITISRANSQMTFPANFQLVAAMNPCPCGYDGDGSGRCRCRAEQIKRYQDKLSGPLLDR
IDLHITVPALPIADLQNAQAGETSEQVRTRVSAAHNRQLIRQNKVNNELSPSEIDKYIQLGAGEQQLLQLAQQRLNLSAR
GYHRVLRVARTIADLADSADITSAHVSEALSYRSK

Specific function: Induced during competence development. Not needed for DNA uptake. May be involved in a recombination step [H]

COG id: COG0606

COG function: function code O; Predicted ATPase with chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Mg-chelatase subunits D/I family. ComM subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693202, Length=520, Percent_Identity=47.5, Blast_Score=451, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001208
- InterPro:   IPR004482
- InterPro:   IPR000523
- InterPro:   IPR020568 [H]

Pfam domain/function: PF01078 Mg_chelatase [H]

EC number: NA

Molecular weight: Translated: 55653; Mature: 55522

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS50051 MCM_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFAQVYTRSVVGLHAPEVIIEVHLSQGLPALTIVGLPEAAVRESKDRVRSAILNSGFQF
CCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
PNRRLTINLAPADLPKDGARLDLPIAIGILAASDQLDPTVLSGFEFIGELALNGELRQVS
CCCEEEEEECCCCCCCCCCEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHH
GSLAVARAMKAEVLATQLLKGDEKMMSSTRQLIVPTINGAEASRVDGITVLAATNLKAVC
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECCHHHHH
NHLQSLTDSSLSDERLAAVEPNQIQQHIGYQVDLADVKGQHHARRALEIAAAGGHSLLLT
HHHHHHHCCCCCCCCEECCCHHHHHHHCCCEEEEHHCCCHHHHHHHHHHEECCCCEEEEE
GPPGSGKTLMASRLPTILPDLSAEDALEVASTYSVADSDYDYGTRPFRQVHHTISAVALV
CCCCCCCEEHHHCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEE
GGGSRPKPGEITLANKGVLFLDELPEFDRTVLEVLRQPLEAKQITISRANSQMTFPANFQ
CCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHCCHHHHEEEEEECCCCEECCCCEE
LVAAMNPCPCGYDGDGSGRCRCRAEQIKRYQDKLSGPLLDRIDLHITVPALPIADLQNAQ
EEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHCCCCC
AGETSEQVRTRVSAAHNRQLIRQNKVNNELSPSEIDKYIQLGAGEQQLLQLAQQRLNLSA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCH
RGYHRVLRVARTIADLADSADITSAHVSEALSYRSK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFAQVYTRSVVGLHAPEVIIEVHLSQGLPALTIVGLPEAAVRESKDRVRSAILNSGFQF
CHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
PNRRLTINLAPADLPKDGARLDLPIAIGILAASDQLDPTVLSGFEFIGELALNGELRQVS
CCCEEEEEECCCCCCCCCCEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHH
GSLAVARAMKAEVLATQLLKGDEKMMSSTRQLIVPTINGAEASRVDGITVLAATNLKAVC
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECCHHHHH
NHLQSLTDSSLSDERLAAVEPNQIQQHIGYQVDLADVKGQHHARRALEIAAAGGHSLLLT
HHHHHHHCCCCCCCCEECCCHHHHHHHCCCEEEEHHCCCHHHHHHHHHHEECCCCEEEEE
GPPGSGKTLMASRLPTILPDLSAEDALEVASTYSVADSDYDYGTRPFRQVHHTISAVALV
CCCCCCCEEHHHCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEE
GGGSRPKPGEITLANKGVLFLDELPEFDRTVLEVLRQPLEAKQITISRANSQMTFPANFQ
CCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHCCHHHHEEEEEECCCCEECCCCEE
LVAAMNPCPCGYDGDGSGRCRCRAEQIKRYQDKLSGPLLDRIDLHITVPALPIADLQNAQ
EEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHCCCCC
AGETSEQVRTRVSAAHNRQLIRQNKVNNELSPSEIDKYIQLGAGEQQLLQLAQQRLNLSA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCH
RGYHRVLRVARTIADLADSADITSAHVSEALSYRSK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 9457884 [H]