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The map label for this gene is priA [H]

Identifier: 93006831

GI number: 93006831

Start: 2467655

End: 2470036

Strand: Direct

Name: priA [H]

Synonym: Pcryo_2007

Alternate gene names: 93006831

Gene position: 2467655-2470036 (Clockwise)

Preceding gene: 93006830

Following gene: 93006832

Centisome position: 80.65

GC content: 45.68

Gene sequence:

>2382_bases
ATGCTAAGCTTATTCGTCTCTCCCTCTTTATTTTTATTTCATATTGACTTAATCAAACTGCGAAGCCTTTTTATGTTGGT
ACAAGTTGCTCTGCCCGTGCCCCTTTATCGGGTATTTGACTATAGCCTACCGTCCGATATAAGTGCTTTGCCAAATGCTT
CGCCAAACAATAGCTCAATACAACTGCCGCAAATTGGCAGTCGTGTTGAGGTGTCCTTTGGTCGTCAGACTTTAATCGGA
ATCGTGGTTGCTCATATTGCGCAAGCGGATACCAGCGTACCACTCAATAAGCTGAAACCCATTAATAAGTGTTTAGATGC
TGAGTCTATTTTAGATAACAGCTTGCTAAAGCTCGCCTATTGGCTTGCTCGTTATTATCATTATCCGCTTGGTGAGGTGT
TAGCGGTCATCTTGCCCACCTTGGTTCGTCAAGGTAAACCGCTAGATTTACTCATTACCCATTGGCGAATTCTGCCACAT
ATCACTGATGACGACTTTCGTACTAACGCCAAAAAGCAAAAGCAGCAGTTTGATATGCTAAAGCTACATGGTCAACATGG
TGCAAGTGAAGATGTACTATTATTAGAAGGTATGGAGCGTGCTTTTTTAAAAACACTAGAAGATAAGGGTTTGATTGAGC
GCTATATTCAACCAAAACAAGCACCTACCTCTGTCAAATTGGCAAAAATGCCCCTTGATCTCAATGAGGAGCAACAGCTT
GCCGTTGCAGCGATTGTTGCTGCACATGAATCCAAATGCTATACGGGCTTTTTATTAAATGGCATTACCGGCAGTGGCAA
GACTGAAGTCTATCTACAAGCCATGCAAGCAGTATTGGAGGCTGGCAAGCAAGTGCTTATTTTAGTGCCTGAGATTGGGT
TAACGCCACAGACGCGTGCGCGTTTTGCCAGTCGCTTCGCTGCGCATATCGTCTTGCTACATTCTGGTATGAACAATACT
CATCGATTGCAAGGTTGGCAAGATTGCCGTACGGGTCACGCACAAATCATTATCGGTACGCGTTCGGCAGTACTATATCC
GTTTGCAGATTTAGGCTTAATTGTCGTTGATGAAGCGCATGATGGCTCCTATAAACAGCAGGATACCTTGCGCTATCATG
CTGCTGATGTCGCACTTTATCGAGGATTCCAAGCCAAAATACCGGTGGTCCTTGGTACGGCTACCCCTTCTCTTGAACAT
TTAAAATTAGTCGATGAGGGCAAGCTAGTAGAGTGTCAATTATCGACGCGCCCTGGCAATGCTAAACCTGCGCCCATGCA
GCTGATTGATGCGCGCTTGCAAAGTACTCATCAGCAAACGCAAGATGATGGCGCGCGCTATGATACCGGACTGACGGATG
CACTTATCGGCGCCATAAGGCAAACCCTAGAAGCTGGCGATCAAGTTTTGATATTTTTAAACCGTCGCGGCTATGCGCCT
GTTTTATTATGCGAGGCGTGTGGTTGGCAGGCAGATTGTCCGCGCTGCGACGCGCATTTAACCGTTCATTATAATAATCA
AGCCCAGCGTAGCAATTATTCAAGCAACTCTTATTTAAAATGCCATCATTGTGATTGGCAAGCCTATATTCCGACTGTAT
GCCCTGATTGTGGCAGTCAAAATCTAGATGCCAAAGGGATGGGAACAACAAGGCTAAGCGAAAATTTGCACGCTATCTTT
GCCAATCCGCAAACCAGTAAAGCGCTTTATCCCATTATTCAAATTGATCGTGATACCACTAGGCGTAAAGACAGCTGGGA
AAATATCTATCAGCGTATTAATGCAGGAAATCCTGCCATATTGGTCGGTACACAAATGGTTGCCAAAGGTCATCATTTTC
CCAATGTCACGCTCGTTTGCTTACCCAACGCTGATCGTGGCTTTTTGTCTGCAGACTTTCGCTCGCCAGAGCATACTGCC
CAATTGATGATCCAAGTAGCGGGGCGTGCAGGACGCGGGGATAAATCAGGACGGGTATTGATTCAAACGCTGCAACCGGA
CAATGCGTTATTATTAAAATTGGTGAAAGACGGTTATTTGTCTTTTGCTCGTGAGCTGTTGCAAGAGCGTAAAATGATGG
GACTGCCCCCTCATAGTCATGCTGCGCTGATACGCTGCGAAGGTAAAACTCTTGCTGCCACCACACAAGCACTCAAAGAT
GCCATTGCCGTTCTGCCAACAGGGCACAATTTAGCCGTTCTTGGTCCTATCGATGCGCCCATGAGTAAAAAGAACAGTCG
CTATCATGCCCAGCTTTTGCTATTGGCCAAGGATCGTCAGCAACTGCATCATGTTTTAAATCATTGGTGGCAACCTGTCC
TTACCCTACCGAGCGCTAAATATCTTAAGCTTACTTTAGATATTGACCCTGTCGGCTGGTAA

Upstream 100 bases:

>100_bases
CGTGGTCGAAATTGAGTCTATTCTATATATTGAATAAGTTAGATATTCAATACTGCTTGAATAACAATATTTCATATTAG
ATCACCATAAGCTTAGCCTC

Downstream 100 bases:

>100_bases
CGCAAATTTGCGAGTTATTGCTAGCATAATCATTTGTCGCATTACTCTTTTATCTTTACTTCGTCAGTACAGTTTCTAAT
ATTTGACTATGATAAATTTA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MLSLFVSPSLFLFHIDLIKLRSLFMLVQVALPVPLYRVFDYSLPSDISALPNASPNNSSIQLPQIGSRVEVSFGRQTLIG
IVVAHIAQADTSVPLNKLKPINKCLDAESILDNSLLKLAYWLARYYHYPLGEVLAVILPTLVRQGKPLDLLITHWRILPH
ITDDDFRTNAKKQKQQFDMLKLHGQHGASEDVLLLEGMERAFLKTLEDKGLIERYIQPKQAPTSVKLAKMPLDLNEEQQL
AVAAIVAAHESKCYTGFLLNGITGSGKTEVYLQAMQAVLEAGKQVLILVPEIGLTPQTRARFASRFAAHIVLLHSGMNNT
HRLQGWQDCRTGHAQIIIGTRSAVLYPFADLGLIVVDEAHDGSYKQQDTLRYHAADVALYRGFQAKIPVVLGTATPSLEH
LKLVDEGKLVECQLSTRPGNAKPAPMQLIDARLQSTHQQTQDDGARYDTGLTDALIGAIRQTLEAGDQVLIFLNRRGYAP
VLLCEACGWQADCPRCDAHLTVHYNNQAQRSNYSSNSYLKCHHCDWQAYIPTVCPDCGSQNLDAKGMGTTRLSENLHAIF
ANPQTSKALYPIIQIDRDTTRRKDSWENIYQRINAGNPAILVGTQMVAKGHHFPNVTLVCLPNADRGFLSADFRSPEHTA
QLMIQVAGRAGRGDKSGRVLIQTLQPDNALLLKLVKDGYLSFARELLQERKMMGLPPHSHAALIRCEGKTLAATTQALKD
AIAVLPTGHNLAVLGPIDAPMSKKNSRYHAQLLLLAKDRQQLHHVLNHWWQPVLTLPSAKYLKLTLDIDPVGW

Sequences:

>Translated_793_residues
MLSLFVSPSLFLFHIDLIKLRSLFMLVQVALPVPLYRVFDYSLPSDISALPNASPNNSSIQLPQIGSRVEVSFGRQTLIG
IVVAHIAQADTSVPLNKLKPINKCLDAESILDNSLLKLAYWLARYYHYPLGEVLAVILPTLVRQGKPLDLLITHWRILPH
ITDDDFRTNAKKQKQQFDMLKLHGQHGASEDVLLLEGMERAFLKTLEDKGLIERYIQPKQAPTSVKLAKMPLDLNEEQQL
AVAAIVAAHESKCYTGFLLNGITGSGKTEVYLQAMQAVLEAGKQVLILVPEIGLTPQTRARFASRFAAHIVLLHSGMNNT
HRLQGWQDCRTGHAQIIIGTRSAVLYPFADLGLIVVDEAHDGSYKQQDTLRYHAADVALYRGFQAKIPVVLGTATPSLEH
LKLVDEGKLVECQLSTRPGNAKPAPMQLIDARLQSTHQQTQDDGARYDTGLTDALIGAIRQTLEAGDQVLIFLNRRGYAP
VLLCEACGWQADCPRCDAHLTVHYNNQAQRSNYSSNSYLKCHHCDWQAYIPTVCPDCGSQNLDAKGMGTTRLSENLHAIF
ANPQTSKALYPIIQIDRDTTRRKDSWENIYQRINAGNPAILVGTQMVAKGHHFPNVTLVCLPNADRGFLSADFRSPEHTA
QLMIQVAGRAGRGDKSGRVLIQTLQPDNALLLKLVKDGYLSFARELLQERKMMGLPPHSHAALIRCEGKTLAATTQALKD
AIAVLPTGHNLAVLGPIDAPMSKKNSRYHAQLLLLAKDRQQLHHVLNHWWQPVLTLPSAKYLKLTLDIDPVGW
>Mature_793_residues
MLSLFVSPSLFLFHIDLIKLRSLFMLVQVALPVPLYRVFDYSLPSDISALPNASPNNSSIQLPQIGSRVEVSFGRQTLIG
IVVAHIAQADTSVPLNKLKPINKCLDAESILDNSLLKLAYWLARYYHYPLGEVLAVILPTLVRQGKPLDLLITHWRILPH
ITDDDFRTNAKKQKQQFDMLKLHGQHGASEDVLLLEGMERAFLKTLEDKGLIERYIQPKQAPTSVKLAKMPLDLNEEQQL
AVAAIVAAHESKCYTGFLLNGITGSGKTEVYLQAMQAVLEAGKQVLILVPEIGLTPQTRARFASRFAAHIVLLHSGMNNT
HRLQGWQDCRTGHAQIIIGTRSAVLYPFADLGLIVVDEAHDGSYKQQDTLRYHAADVALYRGFQAKIPVVLGTATPSLEH
LKLVDEGKLVECQLSTRPGNAKPAPMQLIDARLQSTHQQTQDDGARYDTGLTDALIGAIRQTLEAGDQVLIFLNRRGYAP
VLLCEACGWQADCPRCDAHLTVHYNNQAQRSNYSSNSYLKCHHCDWQAYIPTVCPDCGSQNLDAKGMGTTRLSENLHAIF
ANPQTSKALYPIIQIDRDTTRRKDSWENIYQRINAGNPAILVGTQMVAKGHHFPNVTLVCLPNADRGFLSADFRSPEHTA
QLMIQVAGRAGRGDKSGRVLIQTLQPDNALLLKLVKDGYLSFARELLQERKMMGLPPHSHAALIRCEGKTLAATTQALKD
AIAVLPTGHNLAVLGPIDAPMSKKNSRYHAQLLLLAKDRQQLHHVLNHWWQPVLTLPSAKYLKLTLDIDPVGW

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=775, Percent_Identity=42.3225806451613, Blast_Score=565, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 88005; Mature: 88005

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLFVSPSLFLFHIDLIKLRSLFMLVQVALPVPLYRVFDYSLPSDISALPNASPNNSSI
CEEEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHCCCCCCCCCEE
QLPQIGSRVEVSFGRQTLIGIVVAHIAQADTSVPLNKLKPINKCLDAESILDNSLLKLAY
ECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHCHHHHHHHHHHHHHH
WLARYYHYPLGEVLAVILPTLVRQGKPLDLLITHWRILPHITDDDFRTNAKKQKQQFDML
HHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCHHCCHHHHHHHHHHH
KLHGQHGASEDVLLLEGMERAFLKTLEDKGLIERYIQPKQAPTSVKLAKMPLDLNEEQQL
HHCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCCCCHHH
AVAAIVAAHESKCYTGFLLNGITGSGKTEVYLQAMQAVLEAGKQVLILVPEIGLTPQTRA
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH
RFASRFAAHIVLLHSGMNNTHRLQGWQDCRTGHAQIIIGTRSAVLYPFADLGLIVVDEAH
HHHHHHHHHEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCCEEEEECCCCCEEEEECCC
DGSYKQQDTLRYHAADVALYRGFQAKIPVVLGTATPSLEHLKLVDEGKLVECQLSTRPGN
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHEEEECCCCEEEEEEECCCCC
AKPAPMQLIDARLQSTHQQTQDDGARYDTGLTDALIGAIRQTLEAGDQVLIFLNRRGYAP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
VLLCEACGWQADCPRCDAHLTVHYNNQAQRSNYSSNSYLKCHHCDWQAYIPTVCPDCGSQ
EEEEECCCCCCCCCCCCCEEEEEECCCHHHCCCCCCCEEEEEECCCCEECCCCCCCCCCC
NLDAKGMGTTRLSENLHAIFANPQTSKALYPIIQIDRDTTRRKDSWENIYQRINAGNPAI
CCCCCCCCCHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEE
LVGTQMVAKGHHFPNVTLVCLPNADRGFLSADFRSPEHTAQLMIQVAGRAGRGDKSGRVL
EEECHHHHCCCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
IQTLQPDNALLLKLVKDGYLSFARELLQERKMMGLPPHSHAALIRCEGKTLAATTQALKD
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHH
AIAVLPTGHNLAVLGPIDAPMSKKNSRYHAQLLLLAKDRQQLHHVLNHWWQPVLTLPSAK
HHHEECCCCCEEEEECCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCHHEECCCCE
YLKLTLDIDPVGW
EEEEEEECCCCCC
>Mature Secondary Structure
MLSLFVSPSLFLFHIDLIKLRSLFMLVQVALPVPLYRVFDYSLPSDISALPNASPNNSSI
CEEEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHCCCCCCCCCEE
QLPQIGSRVEVSFGRQTLIGIVVAHIAQADTSVPLNKLKPINKCLDAESILDNSLLKLAY
ECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHCHHHHHHHHHHHHHH
WLARYYHYPLGEVLAVILPTLVRQGKPLDLLITHWRILPHITDDDFRTNAKKQKQQFDML
HHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCHHCCHHHHHHHHHHH
KLHGQHGASEDVLLLEGMERAFLKTLEDKGLIERYIQPKQAPTSVKLAKMPLDLNEEQQL
HHCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCCCCHHH
AVAAIVAAHESKCYTGFLLNGITGSGKTEVYLQAMQAVLEAGKQVLILVPEIGLTPQTRA
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH
RFASRFAAHIVLLHSGMNNTHRLQGWQDCRTGHAQIIIGTRSAVLYPFADLGLIVVDEAH
HHHHHHHHHEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCCEEEEECCCCCEEEEECCC
DGSYKQQDTLRYHAADVALYRGFQAKIPVVLGTATPSLEHLKLVDEGKLVECQLSTRPGN
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHEEEECCCCEEEEEEECCCCC
AKPAPMQLIDARLQSTHQQTQDDGARYDTGLTDALIGAIRQTLEAGDQVLIFLNRRGYAP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
VLLCEACGWQADCPRCDAHLTVHYNNQAQRSNYSSNSYLKCHHCDWQAYIPTVCPDCGSQ
EEEEECCCCCCCCCCCCCEEEEEECCCHHHCCCCCCCEEEEEECCCCEECCCCCCCCCCC
NLDAKGMGTTRLSENLHAIFANPQTSKALYPIIQIDRDTTRRKDSWENIYQRINAGNPAI
CCCCCCCCCHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEE
LVGTQMVAKGHHFPNVTLVCLPNADRGFLSADFRSPEHTAQLMIQVAGRAGRGDKSGRVL
EEECHHHHCCCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
IQTLQPDNALLLKLVKDGYLSFARELLQERKMMGLPPHSHAALIRCEGKTLAATTQALKD
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHH
AIAVLPTGHNLAVLGPIDAPMSKKNSRYHAQLLLLAKDRQQLHHVLNHWWQPVLTLPSAK
HHHEECCCCCEEEEECCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCHHEECCCCE
YLKLTLDIDPVGW
EEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]