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The map label for this gene is aceA [C]

Identifier: 93006684

GI number: 93006684

Start: 2296421

End: 2298007

Strand: Direct

Name: aceA [C]

Synonym: Pcryo_1860

Alternate gene names: 93006684

Gene position: 2296421-2298007 (Clockwise)

Preceding gene: 93006681

Following gene: 93006685

Centisome position: 75.05

GC content: 44.23

Gene sequence:

>1587_bases
ATGTCAACTGCATATAAATCAGCGATCGATTTAGTACGTGAACTAAAACAGAAACACGGTAACTGGCAGAACATCAGCGA
AACTGATGCGGCACGTATGATGGCACAAAACCGCTTCAAAACAGGTTTAGATATTGCTAAGTACACCGCAAAAATTATGC
GTCAAGACATGGAAGAGTATGACAATGACACGTCTCAATACACCCAGTCTCTAGGTGCATGGCATGGTTTTGTTGCACAG
CAAACCATGTATGCGAAGAAAAAATATTTTGGCACAACATCTAAAACTTACATCTACCTATCTGGTTGGATGGTAGCAGC
TCTACGTTCTGAGTTTGGTCCTCTGCCTGACCAATCTATGCACGAAAAAACGTCTGTACCTAAGCTGATTGCAGAGATCT
ACACTTTCTTGCGTCAAGCAGATGCAAAAGTATTGAACGATTATTTCCGCGAGCTAAACGCTGCTGAAGAAGCGGGTCAA
GACACCTCAGCTATCTTAGAAAAAATCGAAAACTTTGATTCACATGTTGTGCCAATCATTGCAGATATCGATGCAGGTTT
CGGTAACGAAGAAGCAACGTATCTATTGACCAAACAAATGATTGAAGCGGGTGCTTGTGCCATTCAGGTTGAAAACCAAG
TATCTGATGCCAAGCAATGTGGTCACCAAGCGGGTAAAGTTACCGTACCGCATGAAGACTTCATCTCTAAAATAAACGCG
ATTCGTTATGCATTCTTAGAGCTTGGTGTTGAAGACGGTGTTATCGTTGCTCGTACTGACTCTGAAGGCGCGTCATTAAC
GCAAAAAATCCCAGTATCAAATGAGCCAGGCGATCTTGCTTCTCAGTACATCGATTTTATCGAGATGGAAGAAGTCACGC
TAGAAAACGCCAAAGAGAACGACAGCTTACTTAAGCATAACGGCAAATTAGTACGTCCAGTTCGTTTGCCTAACGGTTTA
TATCAGTTCCGTGAAGACACCAACATCGATCGCGTGGTCCTTGACTGTGTAACTGCTCTAGAAAACGGCGCTGATCTACT
ATGGATCGAAACACCGACTCCAGACGTAGCACACATCAAAATGATGGTTGACCGTATTAAAGAGCAACAGCCAAAAGCCA
AGCTTGTATACAACAACAGCCCATCGTTCAACTGGACGCTGAACTTCCGTAAGCAAATCATCGCTCAGTGGGAAGAAGAA
GGTAAAGACCTATCTGCCTATAATAAAGCTGACTTGATGAGTGCTGATTACGATGGTACTGAGCTTGATACAGCAGCTGA
CGAAGCGGCTAGAAACTTCCAACGCGATGCAGCACGTGAAGCAGGTGTATTCCATCACTTAATCACGCTACCTACTTATC
ACACCACGGCTCTTAGCGTTCATGAACTTGCTAAAGGCTACTTCGGTGAAGATGGTATGCTTGCTTATGCAGCTGGCGTA
CAACGTAAAGAAATCCGTGAAGGCATCGCTTGTGTTAAACACCAAGCAATGGCGGGTTCTGACCTTGGTGATGATCATAA
AGAGATCTTCTCTGGTGAAAACGCCCTTAAAGCTGGCGGCGGCAAGAACACGATGAACCAGTTCTAA

Upstream 100 bases:

>100_bases
AAGAAGCTTATATTTCAAATTTTTACTTCTGCCAGCATCTTTCTATATAGACCAAAATTATTTTAATTTACTTATTTATT
CACCACCAAGGAGACTATAG

Downstream 100 bases:

>100_bases
TGTCCGACCACTTATAAGTACATTTTATAAGTAAAAGTTTTGAGAAAAGCCGTCCTACATGGTAGGGCGGCTTTTTTGTG
CGCTATAAATAACGATATCT

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSTAYKSAIDLVRELKQKHGNWQNISETDAARMMAQNRFKTGLDIAKYTAKIMRQDMEEYDNDTSQYTQSLGAWHGFVAQ
QTMYAKKKYFGTTSKTYIYLSGWMVAALRSEFGPLPDQSMHEKTSVPKLIAEIYTFLRQADAKVLNDYFRELNAAEEAGQ
DTSAILEKIENFDSHVVPIIADIDAGFGNEEATYLLTKQMIEAGACAIQVENQVSDAKQCGHQAGKVTVPHEDFISKINA
IRYAFLELGVEDGVIVARTDSEGASLTQKIPVSNEPGDLASQYIDFIEMEEVTLENAKENDSLLKHNGKLVRPVRLPNGL
YQFREDTNIDRVVLDCVTALENGADLLWIETPTPDVAHIKMMVDRIKEQQPKAKLVYNNSPSFNWTLNFRKQIIAQWEEE
GKDLSAYNKADLMSADYDGTELDTAADEAARNFQRDAAREAGVFHHLITLPTYHTTALSVHELAKGYFGEDGMLAYAAGV
QRKEIREGIACVKHQAMAGSDLGDDHKEIFSGENALKAGGGKNTMNQF

Sequences:

>Translated_528_residues
MSTAYKSAIDLVRELKQKHGNWQNISETDAARMMAQNRFKTGLDIAKYTAKIMRQDMEEYDNDTSQYTQSLGAWHGFVAQ
QTMYAKKKYFGTTSKTYIYLSGWMVAALRSEFGPLPDQSMHEKTSVPKLIAEIYTFLRQADAKVLNDYFRELNAAEEAGQ
DTSAILEKIENFDSHVVPIIADIDAGFGNEEATYLLTKQMIEAGACAIQVENQVSDAKQCGHQAGKVTVPHEDFISKINA
IRYAFLELGVEDGVIVARTDSEGASLTQKIPVSNEPGDLASQYIDFIEMEEVTLENAKENDSLLKHNGKLVRPVRLPNGL
YQFREDTNIDRVVLDCVTALENGADLLWIETPTPDVAHIKMMVDRIKEQQPKAKLVYNNSPSFNWTLNFRKQIIAQWEEE
GKDLSAYNKADLMSADYDGTELDTAADEAARNFQRDAAREAGVFHHLITLPTYHTTALSVHELAKGYFGEDGMLAYAAGV
QRKEIREGIACVKHQAMAGSDLGDDHKEIFSGENALKAGGGKNTMNQF
>Mature_527_residues
STAYKSAIDLVRELKQKHGNWQNISETDAARMMAQNRFKTGLDIAKYTAKIMRQDMEEYDNDTSQYTQSLGAWHGFVAQQ
TMYAKKKYFGTTSKTYIYLSGWMVAALRSEFGPLPDQSMHEKTSVPKLIAEIYTFLRQADAKVLNDYFRELNAAEEAGQD
TSAILEKIENFDSHVVPIIADIDAGFGNEEATYLLTKQMIEAGACAIQVENQVSDAKQCGHQAGKVTVPHEDFISKINAI
RYAFLELGVEDGVIVARTDSEGASLTQKIPVSNEPGDLASQYIDFIEMEEVTLENAKENDSLLKHNGKLVRPVRLPNGLY
QFREDTNIDRVVLDCVTALENGADLLWIETPTPDVAHIKMMVDRIKEQQPKAKLVYNNSPSFNWTLNFRKQIIAQWEEEG
KDLSAYNKADLMSADYDGTELDTAADEAARNFQRDAAREAGVFHHLITLPTYHTTALSVHELAKGYFGEDGMLAYAAGVQ
RKEIREGIACVKHQAMAGSDLGDDHKEIFSGENALKAGGGKNTMNQF

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=467, Percent_Identity=28.4796573875803, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71982926, Length=474, Percent_Identity=29.957805907173, Blast_Score=148, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17561814, Length=474, Percent_Identity=29.957805907173, Blast_Score=147, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6325263, Length=428, Percent_Identity=25, Blast_Score=113, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6320908, Length=527, Percent_Identity=24.6679316888046, Blast_Score=100, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 58975; Mature: 58844

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAYKSAIDLVRELKQKHGNWQNISETDAARMMAQNRFKTGLDIAKYTAKIMRQDMEEY
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DNDTSQYTQSLGAWHGFVAQQTMYAKKKYFGTTSKTYIYLSGWMVAALRSEFGPLPDQSM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHH
HEKTSVPKLIAEIYTFLRQADAKVLNDYFRELNAAEEAGQDTSAILEKIENFDSHVVPII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEE
ADIDAGFGNEEATYLLTKQMIEAGACAIQVENQVSDAKQCGHQAGKVTVPHEDFISKINA
EECCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCEECCHHHHHHHHHH
IRYAFLELGVEDGVIVARTDSEGASLTQKIPVSNEPGDLASQYIDFIEMEEVTLENAKEN
HHHHHHHHCCCCCEEEEEECCCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
DSLLKHNGKLVRPVRLPNGLYQFREDTNIDRVVLDCVTALENGADLLWIETPTPDVAHIK
CHHHHCCCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
MMVDRIKEQQPKAKLVYNNSPSFNWTLNFRKQIIAQWEEEGKDLSAYNKADLMSADYDGT
HHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCHHCCHHHCCCCCCCCC
ELDTAADEAARNFQRDAAREAGVFHHLITLPTYHTTALSVHELAKGYFGEDGMLAYAAGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCC
QRKEIREGIACVKHQAMAGSDLGDDHKEIFSGENALKAGGGKNTMNQF
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure 
STAYKSAIDLVRELKQKHGNWQNISETDAARMMAQNRFKTGLDIAKYTAKIMRQDMEEY
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DNDTSQYTQSLGAWHGFVAQQTMYAKKKYFGTTSKTYIYLSGWMVAALRSEFGPLPDQSM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHH
HEKTSVPKLIAEIYTFLRQADAKVLNDYFRELNAAEEAGQDTSAILEKIENFDSHVVPII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEE
ADIDAGFGNEEATYLLTKQMIEAGACAIQVENQVSDAKQCGHQAGKVTVPHEDFISKINA
EECCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCEECCHHHHHHHHHH
IRYAFLELGVEDGVIVARTDSEGASLTQKIPVSNEPGDLASQYIDFIEMEEVTLENAKEN
HHHHHHHHCCCCCEEEEEECCCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
DSLLKHNGKLVRPVRLPNGLYQFREDTNIDRVVLDCVTALENGADLLWIETPTPDVAHIK
CHHHHCCCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
MMVDRIKEQQPKAKLVYNNSPSFNWTLNFRKQIIAQWEEEGKDLSAYNKADLMSADYDGT
HHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCHHCCHHHCCCCCCCCC
ELDTAADEAARNFQRDAAREAGVFHHLITLPTYHTTALSVHELAKGYFGEDGMLAYAAGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCC
QRKEIREGIACVKHQAMAGSDLGDDHKEIFSGENALKAGGGKNTMNQF
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]