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The map label for this gene is purN [H]

Identifier: 93006681

GI number: 93006681

Start: 2292049

End: 2292741

Strand: Direct

Name: purN [H]

Synonym: Pcryo_1857

Alternate gene names: 93006681

Gene position: 2292049-2292741 (Clockwise)

Preceding gene: 93006680

Following gene: 93006684

Centisome position: 74.91

GC content: 45.02

Gene sequence:

>693_bases
ATGCTAGCCAATAACACCAGTCATGCCAACAAACCATTACGCATTGCCGTTTTGGTATCTGGTAGCGGTAGCAACTTACA
AGTATTGATTGATGCTATGCAAGCTGGTGCGCTACCAATTGAGATTGTCGGCGTTATTAGTAACCGTGAAGACGCTTATG
CCATCACACGGGCAAAAGATGCTGACATTCCAGTGGCGGTCTTATCTCACGTTGCTAGTGGCAAACGCATGGGAATCAAA
ACTTTTGAGAGTCATGCCAGCGCCCAACTAACCACATGGCAACCTGACTTGATTGTGTTAGCTGGTTTTATGCGTGTTTT
AAGTGCTGGTTTTATTGATAACACGCCAGCACCAATGATTAATCTGCATCCTGCACTCTTGCCTGCTTATAAAGGTTTGG
ATACTCATCAGCGTGTCATTCAAGCAGGTGAGCGTCAGCACGGCTGTAGCATTCATGTCGTTACCGCTGAGCTCGATGCA
GGTGCTGTTTTGACCCAAGCCTGGCTAGAAGTGCATCAAAAAGATACTGCTGACAGTTTGCAAACGCGCGTGCAAAAGCT
TGAACATCAGCTGTTACCTTGGACAATTTTGTTATTAGCCAAAGGTGTATTGTCGTTGAATAACGAGCAATTATTAAATC
AGCAGCGCACCTGTATGCCGACGCTACCTTTGAAGCTATATTTAAACAGTTAA

Upstream 100 bases:

>100_bases
AAGCCGAAGCTGCTATCCAAACCTTGAATGATGCTGGCGAAAAAGCATGGAAATTAGGTGAAATGGTCAAGCGCGAAGCG
GATGCTGTGGTGTACCGTTA

Downstream 100 bases:

>100_bases
AGCAATGGCAACAAGCAAAGAAAAGTAGCACTCATTTCAAACTATCGACCAAAAAAAGCCCAGTCACTCAATTTATGAAT
GACTGGGCTTTTCGCTACTT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MLANNTSHANKPLRIAVLVSGSGSNLQVLIDAMQAGALPIEIVGVISNREDAYAITRAKDADIPVAVLSHVASGKRMGIK
TFESHASAQLTTWQPDLIVLAGFMRVLSAGFIDNTPAPMINLHPALLPAYKGLDTHQRVIQAGERQHGCSIHVVTAELDA
GAVLTQAWLEVHQKDTADSLQTRVQKLEHQLLPWTILLLAKGVLSLNNEQLLNQQRTCMPTLPLKLYLNS

Sequences:

>Translated_230_residues
MLANNTSHANKPLRIAVLVSGSGSNLQVLIDAMQAGALPIEIVGVISNREDAYAITRAKDADIPVAVLSHVASGKRMGIK
TFESHASAQLTTWQPDLIVLAGFMRVLSAGFIDNTPAPMINLHPALLPAYKGLDTHQRVIQAGERQHGCSIHVVTAELDA
GAVLTQAWLEVHQKDTADSLQTRVQKLEHQLLPWTILLLAKGVLSLNNEQLLNQQRTCMPTLPLKLYLNS
>Mature_230_residues
MLANNTSHANKPLRIAVLVSGSGSNLQVLIDAMQAGALPIEIVGVISNREDAYAITRAKDADIPVAVLSHVASGKRMGIK
TFESHASAQLTTWQPDLIVLAGFMRVLSAGFIDNTPAPMINLHPALLPAYKGLDTHQRVIQAGERQHGCSIHVVTAELDA
GAVLTQAWLEVHQKDTADSLQTRVQKLEHQLLPWTILLLAKGVLSLNNEQLLNQQRTCMPTLPLKLYLNS

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=207, Percent_Identity=36.231884057971, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI209869995, Length=207, Percent_Identity=36.231884057971, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI209869993, Length=207, Percent_Identity=36.231884057971, Blast_Score=144, Evalue=9e-35,
Organism=Escherichia coli, GI1788846, Length=205, Percent_Identity=38.5365853658537, Blast_Score=153, Evalue=8e-39,
Organism=Escherichia coli, GI1787483, Length=200, Percent_Identity=27, Blast_Score=84, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=194, Percent_Identity=34.020618556701, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=34.6938775510204, Blast_Score=96, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24582400, Length=198, Percent_Identity=39.8989898989899, Blast_Score=146, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 24952; Mature: 24952

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLANNTSHANKPLRIAVLVSGSGSNLQVLIDAMQAGALPIEIVGVISNREDAYAITRAKD
CCCCCCCCCCCCEEEEEEEECCCCCHHHHEEHHHCCCCCEEEEEEECCCCCCEEEEECCC
ADIPVAVLSHVASGKRMGIKTFESHASAQLTTWQPDLIVLAGFMRVLSAGFIDNTPAPMI
CCCHHHHHHHHHCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEE
NLHPALLPAYKGLDTHQRVIQAGERQHGCSIHVVTAELDAGAVLTQAWLEVHQKDTADSL
ECCHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHH
QTRVQKLEHQLLPWTILLLAKGVLSLNNEQLLNQQRTCMPTLPLKLYLNS
HHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure
MLANNTSHANKPLRIAVLVSGSGSNLQVLIDAMQAGALPIEIVGVISNREDAYAITRAKD
CCCCCCCCCCCCEEEEEEEECCCCCHHHHEEHHHCCCCCEEEEEEECCCCCCEEEEECCC
ADIPVAVLSHVASGKRMGIKTFESHASAQLTTWQPDLIVLAGFMRVLSAGFIDNTPAPMI
CCCHHHHHHHHHCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEE
NLHPALLPAYKGLDTHQRVIQAGERQHGCSIHVVTAELDAGAVLTQAWLEVHQKDTADSL
ECCHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHH
QTRVQKLEHQLLPWTILLLAKGVLSLNNEQLLNQQRTCMPTLPLKLYLNS
HHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]