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The map label for this gene is ispB [H]

Identifier: 93006580

GI number: 93006580

Start: 2171025

End: 2172059

Strand: Direct

Name: ispB [H]

Synonym: Pcryo_1756

Alternate gene names: 93006580

Gene position: 2171025-2172059 (Clockwise)

Preceding gene: 93006576

Following gene: 93006581

Centisome position: 70.95

GC content: 44.93

Gene sequence:

>1035_bases
ATGACTTGTACTCCTTTATCTAAAACCGCTGCGCCATTAATCAATCTATCGTCTACACCAACCTATGCTGATATCCAGAG
CATTGTGGCTGATGATTTCGATATTATGGACAAACAAGTGTTTGGCAGCCTTAATTCCAAAGTTAAGCTGGTGATGAATG
TCTCACAGCACGTGATTAACGCTGGCGGCAAACGTATGCGCCCATTAATTACCTTGTTATGCGCGCGAATGCTTAATGAT
GAGCCATCGAAGCAAGCCATGCATTTGGCAGCGATTACTGAGATGCTGCATACGGCAACCTTGGTACATGATGATGTGAT
TGATGAATCAGGACAGCGCCGTGGTAAGCCTACTGCCAATGCCACTTGGGATAATGCGACAGCTGTCTTGGTCGGCGATT
ATTTGATTGCTCGCGCTTTTAATTTGTTAGTGGGCTTTCAAAGCTTGCCACTATTGCAAGTGTTTTCAGACGGTACTTGT
GATATTGCTGAAGGTGAAGTATTACAACTGCAGCATCAGCATAATCCTGAAGCAACTGAGCAAGATTACCTCAATATCAT
CGATGGTAAGACTTCACGATTATTTATGATGGCAACTCAAGGCGCTGCGATTTTGCAAGGTCAAACGCAGCATTTGCAAG
CATTGGCTAATTTTGGTCAGCACTTTGGCAATGCTTTTCAAATTGTCGATGACGTGCTTGATTACAGTGGCGATAGTGCG
CTGATGGGTAAAAATCTCGGCGATGATCTAGCTGAAGGCAAACCTACTCTACCTACTATTAAAGCACTCGAACTACTCAA
AGACAGCAACAAAGAAGGCTATGAGCAGCTGCGTATCGCCGTCCAAACGGGCAAAACCCCCAATGCAGAACAGTTAATCG
CCTTAGTACGCCATTCAGGCTCACTAGATTACTGTAAACAGCGGGCTTTAGAAGAAACAAAGCTTGCCCAACAAGCACTC
AGCAGTTTGCCGGATAATCGCTATCGTCAGGGCCTATACCAGTTGACTGAACTTGCCAGTGCTCGCTTATTATAA

Upstream 100 bases:

>100_bases
AGCTATGCTATCATGCATAAAACGCTGGCTTTGCTAAGTATCACTATTAAAAGTATCGCTATTAAAATATTCTTTATTTT
TATTGGTATGATTATCTATT

Downstream 100 bases:

>100_bases
TTATCAAATAACAACGCTTAGTTATTATTTATATTGCTACTACATTGATAAGACTCAAATCGATACTGCTTATATTGATA
TAAGCAGTTAAATGTTTTTA

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTCTPLSKTAAPLINLSSTPTYADIQSIVADDFDIMDKQVFGSLNSKVKLVMNVSQHVINAGGKRMRPLITLLCARMLND
EPSKQAMHLAAITEMLHTATLVHDDVIDESGQRRGKPTANATWDNATAVLVGDYLIARAFNLLVGFQSLPLLQVFSDGTC
DIAEGEVLQLQHQHNPEATEQDYLNIIDGKTSRLFMMATQGAAILQGQTQHLQALANFGQHFGNAFQIVDDVLDYSGDSA
LMGKNLGDDLAEGKPTLPTIKALELLKDSNKEGYEQLRIAVQTGKTPNAEQLIALVRHSGSLDYCKQRALEETKLAQQAL
SSLPDNRYRQGLYQLTELASARLL

Sequences:

>Translated_344_residues
MTCTPLSKTAAPLINLSSTPTYADIQSIVADDFDIMDKQVFGSLNSKVKLVMNVSQHVINAGGKRMRPLITLLCARMLND
EPSKQAMHLAAITEMLHTATLVHDDVIDESGQRRGKPTANATWDNATAVLVGDYLIARAFNLLVGFQSLPLLQVFSDGTC
DIAEGEVLQLQHQHNPEATEQDYLNIIDGKTSRLFMMATQGAAILQGQTQHLQALANFGQHFGNAFQIVDDVLDYSGDSA
LMGKNLGDDLAEGKPTLPTIKALELLKDSNKEGYEQLRIAVQTGKTPNAEQLIALVRHSGSLDYCKQRALEETKLAQQAL
SSLPDNRYRQGLYQLTELASARLL
>Mature_343_residues
TCTPLSKTAAPLINLSSTPTYADIQSIVADDFDIMDKQVFGSLNSKVKLVMNVSQHVINAGGKRMRPLITLLCARMLNDE
PSKQAMHLAAITEMLHTATLVHDDVIDESGQRRGKPTANATWDNATAVLVGDYLIARAFNLLVGFQSLPLLQVFSDGTCD
IAEGEVLQLQHQHNPEATEQDYLNIIDGKTSRLFMMATQGAAILQGQTQHLQALANFGQHFGNAFQIVDDVLDYSGDSAL
MGKNLGDDLAEGKPTLPTIKALELLKDSNKEGYEQLRIAVQTGKTPNAEQLIALVRHSGSLDYCKQRALEETKLAQQALS
SLPDNRYRQGLYQLTELASARLL

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=298, Percent_Identity=30.5369127516779, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4758430, Length=216, Percent_Identity=24.537037037037, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI83700220, Length=216, Percent_Identity=24.537037037037, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1789578, Length=320, Percent_Identity=41.5625, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1786623, Length=238, Percent_Identity=29.8319327731092, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17505681, Length=302, Percent_Identity=30.794701986755, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319475, Length=297, Percent_Identity=30.976430976431, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325188, Length=262, Percent_Identity=25.9541984732824, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24651612, Length=302, Percent_Identity=29.8013245033113, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281365769, Length=228, Percent_Identity=24.5614035087719, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24660002, Length=228, Percent_Identity=24.5614035087719, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 37633; Mature: 37502

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCTPLSKTAAPLINLSSTPTYADIQSIVADDFDIMDKQVFGSLNSKVKLVMNVSQHVIN
CCCCCCCHHHHCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AGGKRMRPLITLLCARMLNDEPSKQAMHLAAITEMLHTATLVHDDVIDESGQRRGKPTAN
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ATWDNATAVLVGDYLIARAFNLLVGFQSLPLLQVFSDGTCDIAEGEVLQLQHQHNPEATE
CCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEECCCCCCCCH
QDYLNIIDGKTSRLFMMATQGAAILQGQTQHLQALANFGQHFGNAFQIVDDVLDYSGDSA
HHHHHHHCCCCCEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LMGKNLGDDLAEGKPTLPTIKALELLKDSNKEGYEQLRIAVQTGKTPNAEQLIALVRHSG
HHCCCCCCHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
SLDYCKQRALEETKLAQQALSSLPDNRYRQGLYQLTELASARLL
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TCTPLSKTAAPLINLSSTPTYADIQSIVADDFDIMDKQVFGSLNSKVKLVMNVSQHVIN
CCCCCCHHHHCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AGGKRMRPLITLLCARMLNDEPSKQAMHLAAITEMLHTATLVHDDVIDESGQRRGKPTAN
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ATWDNATAVLVGDYLIARAFNLLVGFQSLPLLQVFSDGTCDIAEGEVLQLQHQHNPEATE
CCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEECCCCCCCCH
QDYLNIIDGKTSRLFMMATQGAAILQGQTQHLQALANFGQHFGNAFQIVDDVLDYSGDSA
HHHHHHHCCCCCEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LMGKNLGDDLAEGKPTLPTIKALELLKDSNKEGYEQLRIAVQTGKTPNAEQLIALVRHSG
HHCCCCCCHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
SLDYCKQRALEETKLAQQALSSLPDNRYRQGLYQLTELASARLL
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]