Click here to switch to the map view.

The map label for this gene is mexA [H]

Identifier: 93006581

GI number: 93006581

Start: 2172607

End: 2173953

Strand: Direct

Name: mexA [H]

Synonym: Pcryo_1757

Alternate gene names: 93006581

Gene position: 2172607-2173953 (Clockwise)

Preceding gene: 93006580

Following gene: 93006582

Centisome position: 71.0

GC content: 48.55

Gene sequence:

>1347_bases
ATGAAGCACTCTTATCTTGCGCTTGTAATAGCAACTGTACTATCTGGAGCTGTACTGGTTGGCTGTGACAAAAAAGAGGA
TGCCGCTGGCGACGCGGCGGCGGCACAGCAACAAATGCCGCCCTCCGTCGTCAACGTTCAGACTGTTACTTTTGATACCG
TACCACAAGTACAGACTTTTGCTGGACGTACAGCGGCTTATCAAACCGCTGACGTCCGCCCACAGGTAAACGGCATTATC
GACGAAGTGTTGTTCCGTGAGGGCAGCAACGTCAAAAAAGGTCAACCTTTATATCGCATTAATACGGATAACTATGCGAC
GTCTATTACCAGTGGTCAGGCGGCAATAGCCCAAGCAGAGGCCAACTATCAGACGGCATTGGCTAATAATGCCAATGCAA
AAGCGGATTTGGCCAGCCGTCAAGCGTCATTATCACAAGCACAAAATGATTTGCAACGTCTGCAAGGTTTGGTGGCTATT
GATGCCATCTCAAAGCAGCAATACGATCAAGCACAAACCGCTGTGCGTACTGCACAAGCAGCTGTCCAAAGCGCCAATGC
TGCTATCGGTCAATCACAAGCCGGTATTGAAAGCGCAAAAGCAGGTATCCAAACGGCGAAGGCTGGGTTGCAAGCGAGCA
CTTTAGATCTCAATCGTACTATCGTACGTGCGCCTATCTCAGGTCGTACAGATCGCTCGAGCGTCACTGCCGGTACATTG
GTTAGCTCAGGTCAACCTACTCCTTTAGTGACTATCTCACGCCTAGATCCTATCTATGTCGATATCAGTCAATCATCCTC
TGAGCTGCTTAAATTGCGTCAGCAGATCTCTGCAGGCAAAGCAAAAACTGGCATGAACTCAGTTGAATTGGTGTTAGAGG
ATGGTTCAACCTATCCTGTACGTGGCGAGTTGGCATTGTCAGAGGCGAAAGTCGATGAATCAACAGGGGCAGTGACCTTA
CGTGCCATCTTCCCAAATAACAGCAATATCTTATTACCCGGTATGTATGTCACTGCGCGCTTAACTCAAAGCGTCATTAC
CAATGCGGCATTAATTCCGCAAAGCGCAGTCACACGTACACCTAAGAGTGAAACGCAAGTCTATATCGTCGATGAAAACA
ACAAAATCCAAGTTCGTCCGGTCACGATTAATGGTACTTATCAAGGACGATGGGTAGTCACTGACGGTCTTCAAGCTGGC
GACAAAGTCGTGATTATCGGCGGCGCAAAAGTGAAGCCTGAACAAGAGGTTATTGCGAAGCCATTAGAAAATCCAAACCC
TGCGCCTGCTGCAAAAGAAGCTGCTGCCAAAACACCGCAGCAAGCTGCACAATCAGCCGCCAAATAA

Upstream 100 bases:

>100_bases
CCTAATAGTTTTTTATATTTTCTACGTTAAGATGTATCCATAAACTTTTTATATTGTCACTATTTATTATTATATGATAT
TTATATTGCCGAGGACATTG

Downstream 100 bases:

>100_bases
TTCCATTCAAAGCTAGGAAGACTTAGGGATTTACTATGTCACGTTTTTTTATTGATCGCCCTATTTTTGCATGGGTGATG
GCTATTTTAGTCATGCTTAT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKHSYLALVIATVLSGAVLVGCDKKEDAAGDAAAAQQQMPPSVVNVQTVTFDTVPQVQTFAGRTAAYQTADVRPQVNGII
DEVLFREGSNVKKGQPLYRINTDNYATSITSGQAAIAQAEANYQTALANNANAKADLASRQASLSQAQNDLQRLQGLVAI
DAISKQQYDQAQTAVRTAQAAVQSANAAIGQSQAGIESAKAGIQTAKAGLQASTLDLNRTIVRAPISGRTDRSSVTAGTL
VSSGQPTPLVTISRLDPIYVDISQSSSELLKLRQQISAGKAKTGMNSVELVLEDGSTYPVRGELALSEAKVDESTGAVTL
RAIFPNNSNILLPGMYVTARLTQSVITNAALIPQSAVTRTPKSETQVYIVDENNKIQVRPVTINGTYQGRWVVTDGLQAG
DKVVIIGGAKVKPEQEVIAKPLENPNPAPAAKEAAAKTPQQAAQSAAK

Sequences:

>Translated_448_residues
MKHSYLALVIATVLSGAVLVGCDKKEDAAGDAAAAQQQMPPSVVNVQTVTFDTVPQVQTFAGRTAAYQTADVRPQVNGII
DEVLFREGSNVKKGQPLYRINTDNYATSITSGQAAIAQAEANYQTALANNANAKADLASRQASLSQAQNDLQRLQGLVAI
DAISKQQYDQAQTAVRTAQAAVQSANAAIGQSQAGIESAKAGIQTAKAGLQASTLDLNRTIVRAPISGRTDRSSVTAGTL
VSSGQPTPLVTISRLDPIYVDISQSSSELLKLRQQISAGKAKTGMNSVELVLEDGSTYPVRGELALSEAKVDESTGAVTL
RAIFPNNSNILLPGMYVTARLTQSVITNAALIPQSAVTRTPKSETQVYIVDENNKIQVRPVTINGTYQGRWVVTDGLQAG
DKVVIIGGAKVKPEQEVIAKPLENPNPAPAAKEAAAKTPQQAAQSAAK
>Mature_448_residues
MKHSYLALVIATVLSGAVLVGCDKKEDAAGDAAAAQQQMPPSVVNVQTVTFDTVPQVQTFAGRTAAYQTADVRPQVNGII
DEVLFREGSNVKKGQPLYRINTDNYATSITSGQAAIAQAEANYQTALANNANAKADLASRQASLSQAQNDLQRLQGLVAI
DAISKQQYDQAQTAVRTAQAAVQSANAAIGQSQAGIESAKAGIQTAKAGLQASTLDLNRTIVRAPISGRTDRSSVTAGTL
VSSGQPTPLVTISRLDPIYVDISQSSSELLKLRQQISAGKAKTGMNSVELVLEDGSTYPVRGELALSEAKVDESTGAVTL
RAIFPNNSNILLPGMYVTARLTQSVITNAALIPQSAVTRTPKSETQVYIVDENNKIQVRPVTINGTYQGRWVVTDGLQAG
DKVVIIGGAKVKPEQEVIAKPLENPNPAPAAKEAAAKTPQQAAQSAAK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=447, Percent_Identity=39.1498881431767, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1789665, Length=423, Percent_Identity=38.5342789598109, Blast_Score=254, Evalue=6e-69,
Organism=Escherichia coli, GI1789929, Length=408, Percent_Identity=36.7647058823529, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI87082036, Length=405, Percent_Identity=26.6666666666667, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790519, Length=291, Percent_Identity=25.7731958762887, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 47012; Mature: 47012

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSYLALVIATVLSGAVLVGCDKKEDAAGDAAAAQQQMPPSVVNVQTVTFDTVPQVQTF
CCCHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCHHHH
AGRTAAYQTADVRPQVNGIIDEVLFREGSNVKKGQPLYRINTDNYATSITSGQAAIAQAE
CCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHC
ANYQTALANNANAKADLASRQASLSQAQNDLQRLQGLVAIDAISKQQYDQAQTAVRTAQA
CCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVQSANAAIGQSQAGIESAKAGIQTAKAGLQASTLDLNRTIVRAPISGRTDRSSVTAGTL
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCCEECCE
VSSGQPTPLVTISRLDPIYVDISQSSSELLKLRQQISAGKAKTGMNSVELVLEDGSTYPV
ECCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCE
RGELALSEAKVDESTGAVTLRAIFPNNSNILLPGMYVTARLTQSVITNAALIPQSAVTRT
ECCEEECHHCCCCCCCEEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHCCCHHHCCC
PKSETQVYIVDENNKIQVRPVTINGTYQGRWVVTDGLQAGDKVVIIGGAKVKPEQEVIAK
CCCCCEEEEEECCCEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHC
PLENPNPAPAAKEAAAKTPQQAAQSAAK
CCCCCCCCCHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MKHSYLALVIATVLSGAVLVGCDKKEDAAGDAAAAQQQMPPSVVNVQTVTFDTVPQVQTF
CCCHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCHHHH
AGRTAAYQTADVRPQVNGIIDEVLFREGSNVKKGQPLYRINTDNYATSITSGQAAIAQAE
CCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHC
ANYQTALANNANAKADLASRQASLSQAQNDLQRLQGLVAIDAISKQQYDQAQTAVRTAQA
CCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVQSANAAIGQSQAGIESAKAGIQTAKAGLQASTLDLNRTIVRAPISGRTDRSSVTAGTL
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCCEECCE
VSSGQPTPLVTISRLDPIYVDISQSSSELLKLRQQISAGKAKTGMNSVELVLEDGSTYPV
ECCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCE
RGELALSEAKVDESTGAVTLRAIFPNNSNILLPGMYVTARLTQSVITNAALIPQSAVTRT
ECCEEECHHCCCCCCCEEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHCCCHHHCCC
PKSETQVYIVDENNKIQVRPVTINGTYQGRWVVTDGLQAGDKVVIIGGAKVKPEQEVIAK
CCCCCEEEEEECCCEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHC
PLENPNPAPAAKEAAAKTPQQAAQSAAK
CCCCCCCCCHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]