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The map label for this gene is mrcB [H]

Identifier: 93006470

GI number: 93006470

Start: 2027215

End: 2029698

Strand: Direct

Name: mrcB [H]

Synonym: Pcryo_1646

Alternate gene names: 93006470

Gene position: 2027215-2029698 (Clockwise)

Preceding gene: 93006468

Following gene: 93006471

Centisome position: 66.25

GC content: 45.21

Gene sequence:

>2484_bases
GTGCAAAAATCTAATTCTAAGCAACCACAGTCCTCTAAGCCTGCTATCAATACGCTACCTTCCCAGCAGCGCGGTGTGGT
GTTTAGTAGTATTTTATTAATTGTTGTCATCGCTGGCATGGTGCTATTAGCACTATACTTGGTCAAACTTGATCGCACCA
TTACTCACAAGTTTGAGGGTAAACGCTGGGATATTCCTGCCAAAGTATATTCGCAGCCATTAGAGCTGTATCAAGGGGCC
AACGTCGATAAAGACACGATGAAGAAATGGCTTGAGCTACTTAATTATCAAAGTGATAAAGCCTATGATCGCACGGGTAC
TTATCATAAATCGGGTAATACCTATTTTATTCATACACGTGGGTTTACTTATAGCGCTAATGATATAGATCAAGAGCAAG
TCATCAAAATGACTATTGCTGGTGATAAGATTGAGTCAGTACAAAGTACTGTGGCGGCGAAAACCGGCATTATCCGTCTT
GAACCGGTGAATATCGGTGGTATTTACCCAGATAGTAACGAAGACCGTATGGTTGTCTCGTTAGATCAAGTACCCCAGCC
GCTTATTGATGCGCTGATAGCGACAGAAGATCGTGGTTTTTATGAACATAAAGGGGTATCGATACGTGGTATCGCTCGTG
CAGTTATCAATAACTTCTCTGGTGGATCACGTCAAGGTGGCTCGACCATCACCCAGCAGCTGATCAAAAACTTTTATCTG
AATTCAGACCGCACAATGAAGCGTAAAGCCAATGAAGCCTTGATGGCTGTGCTACTTGAGCTGCATTATAGTAAAGATGA
GATTTTGCAAGCATATCTCAACGAGATTTATCTTGGTCAAAATGGCAAACGCTCTATTAATGGTTTTGGGTTGGCATCAC
AATTTTATTTTGATAAGCCTCTCAATGAGCTGCGCCTAGATCAGCAAGCATTGCTTGTCGGTATGGCAAAAGGACCGAGC
GTTTATAATCCGCGCCGTCATCCAAACGATTCAAAGGCGCGTCGCGATGTTGTGCTTAGCAATATGTTGGCCGTCGGCTC
GCTCAGTCAAGAAGACTACGATAAAGCATTAAAAAACTCACTTGGGGTAGTTGATAAGCCTGTTGAAGGTAAAAGCCAAT
TCCCTGACTTTTTAGATATCGTCAAGCGTGAACTAAATAAAGTCTATTATTCCGATGATCTCAAAAATGAAGGTCTGATT
ATCATCAGTACGATGGACCCTATTGCCCAGTTGGCAGCGGACAAAGCGGTTGAGAGAAAGCTTGGCGAGCTACGCCGTAG
TGGTAGCAAAACCAAAGATTTACAAGGGGCACTGGTCAGTGCCAATCCTGAGACGGGTGAGCTGGTTTCTGTTGTCGGTA
GCGGCAGCGAGTTTACTGGCTTTAACCGTGCGGTCGATGCCAAACGCCAAGTAGGCTCATTATTAAAGCCGATTATTTAT
ATGACTGCTCTCGAAAGTGGTCGCTATAATTTGGCAAGTTCGGTTGATGACTCCCCTATTACGGTCAGTCTAAGCGACGG
TACCGAATGGAATCCCAAAAACTATGACAATCGTGATCATGGCTACATACCCTTTACCACAGCGCTGTCGCAATCGTATA
ACCATAGTGCCGTACGCTTAGGGATGGAGTTTGGAGTCGACACCTTTGCCAAGCAATTAAAACGTATGGGCGTTAAAGAG
AAAATACCTCCTTATCCTGCGGCATTATTGGGCTCAGTCAATCTAAGCCCGATGGACATGCTTGGGGTCTATCAAGTTTT
TGCAACGGGTGGTTTCCGTACGCCTATTCATAGTATCCGTAGTGTGATTGATGATCGCGGTAGTATTTTGCAGCGCACAG
GCTTAAATACTCAGCGCAGTATTCCGCCTGAGACCAACTTTTTGATTAATTATGCGTTGCAAGATGTCGTCAAGAACGGT
ACAGCTAGACGCGTGCAATCGCTTGGTAGCAATCTAAATCTTGCGGGCAAAACAGGTACTACCAATGATTACCGCGATGC
GTGGTTTGCCGGTTATAGTGGCAACTATGTCAGCGTGGTTTGGGTTGGACGTGATGATAATAAACCGATTGGGCTTAGTG
GCGGTAGCGGTGCTTTGCCAGTTTGGGTTGATTATATGAATCGTCTAAAACTGACACCTGTTGCGCTACCAGAGCCAGAA
GGCATCGAATGGCTCTGGTTAGAGAACAACTCAGGCAAGCTGTCTAATGAGCGCTGTGCCAGCGCACGCTACTTGCCTGT
TATGTCAGCATATTTGCCCCAAGAAGCCAGCAGCTGTGCGATTGGCTTATACCAACAGGATCGTGCGCGCGAGCAAATGC
AGTGGCAAAATCAGCAAGGCGATATCAATAAGCAACGTCGCCGTGAAGGATTAGACATACCAGATGGTGAAGTTATTGAT
AATAATAGCAATGATGCCGAGCAAGACGGTGATACACCCAATCGTGTAGATACTTGGTATGATCGTGCTGTTGAATGGTT
TTAG

Upstream 100 bases:

>100_bases
TGAGAGCAAATGAATGCCTGCGTTGATGATGATAGATTGAAAAATTTGTCAATTTGCGTCATTATGAGGCAGTTTTTCAT
CCCAAAATGGTGTGTCATCA

Downstream 100 bases:

>100_bases
TGAACTGCTTCTATTTCAGTCGTGATAGCTGAAAATTTGACTTTAAAAGGTTAGTAGCTGAACGATTTTAAGATTTTTAA
AACTTAGAAAGGGTTTTAAT

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 827; Mature: 827

Protein sequence:

>827_residues
MQKSNSKQPQSSKPAINTLPSQQRGVVFSSILLIVVIAGMVLLALYLVKLDRTITHKFEGKRWDIPAKVYSQPLELYQGA
NVDKDTMKKWLELLNYQSDKAYDRTGTYHKSGNTYFIHTRGFTYSANDIDQEQVIKMTIAGDKIESVQSTVAAKTGIIRL
EPVNIGGIYPDSNEDRMVVSLDQVPQPLIDALIATEDRGFYEHKGVSIRGIARAVINNFSGGSRQGGSTITQQLIKNFYL
NSDRTMKRKANEALMAVLLELHYSKDEILQAYLNEIYLGQNGKRSINGFGLASQFYFDKPLNELRLDQQALLVGMAKGPS
VYNPRRHPNDSKARRDVVLSNMLAVGSLSQEDYDKALKNSLGVVDKPVEGKSQFPDFLDIVKRELNKVYYSDDLKNEGLI
IISTMDPIAQLAADKAVERKLGELRRSGSKTKDLQGALVSANPETGELVSVVGSGSEFTGFNRAVDAKRQVGSLLKPIIY
MTALESGRYNLASSVDDSPITVSLSDGTEWNPKNYDNRDHGYIPFTTALSQSYNHSAVRLGMEFGVDTFAKQLKRMGVKE
KIPPYPAALLGSVNLSPMDMLGVYQVFATGGFRTPIHSIRSVIDDRGSILQRTGLNTQRSIPPETNFLINYALQDVVKNG
TARRVQSLGSNLNLAGKTGTTNDYRDAWFAGYSGNYVSVVWVGRDDNKPIGLSGGSGALPVWVDYMNRLKLTPVALPEPE
GIEWLWLENNSGKLSNERCASARYLPVMSAYLPQEASSCAIGLYQQDRAREQMQWQNQQGDINKQRRREGLDIPDGEVID
NNSNDAEQDGDTPNRVDTWYDRAVEWF

Sequences:

>Translated_827_residues
MQKSNSKQPQSSKPAINTLPSQQRGVVFSSILLIVVIAGMVLLALYLVKLDRTITHKFEGKRWDIPAKVYSQPLELYQGA
NVDKDTMKKWLELLNYQSDKAYDRTGTYHKSGNTYFIHTRGFTYSANDIDQEQVIKMTIAGDKIESVQSTVAAKTGIIRL
EPVNIGGIYPDSNEDRMVVSLDQVPQPLIDALIATEDRGFYEHKGVSIRGIARAVINNFSGGSRQGGSTITQQLIKNFYL
NSDRTMKRKANEALMAVLLELHYSKDEILQAYLNEIYLGQNGKRSINGFGLASQFYFDKPLNELRLDQQALLVGMAKGPS
VYNPRRHPNDSKARRDVVLSNMLAVGSLSQEDYDKALKNSLGVVDKPVEGKSQFPDFLDIVKRELNKVYYSDDLKNEGLI
IISTMDPIAQLAADKAVERKLGELRRSGSKTKDLQGALVSANPETGELVSVVGSGSEFTGFNRAVDAKRQVGSLLKPIIY
MTALESGRYNLASSVDDSPITVSLSDGTEWNPKNYDNRDHGYIPFTTALSQSYNHSAVRLGMEFGVDTFAKQLKRMGVKE
KIPPYPAALLGSVNLSPMDMLGVYQVFATGGFRTPIHSIRSVIDDRGSILQRTGLNTQRSIPPETNFLINYALQDVVKNG
TARRVQSLGSNLNLAGKTGTTNDYRDAWFAGYSGNYVSVVWVGRDDNKPIGLSGGSGALPVWVDYMNRLKLTPVALPEPE
GIEWLWLENNSGKLSNERCASARYLPVMSAYLPQEASSCAIGLYQQDRAREQMQWQNQQGDINKQRRREGLDIPDGEVID
NNSNDAEQDGDTPNRVDTWYDRAVEWF
>Mature_827_residues
MQKSNSKQPQSSKPAINTLPSQQRGVVFSSILLIVVIAGMVLLALYLVKLDRTITHKFEGKRWDIPAKVYSQPLELYQGA
NVDKDTMKKWLELLNYQSDKAYDRTGTYHKSGNTYFIHTRGFTYSANDIDQEQVIKMTIAGDKIESVQSTVAAKTGIIRL
EPVNIGGIYPDSNEDRMVVSLDQVPQPLIDALIATEDRGFYEHKGVSIRGIARAVINNFSGGSRQGGSTITQQLIKNFYL
NSDRTMKRKANEALMAVLLELHYSKDEILQAYLNEIYLGQNGKRSINGFGLASQFYFDKPLNELRLDQQALLVGMAKGPS
VYNPRRHPNDSKARRDVVLSNMLAVGSLSQEDYDKALKNSLGVVDKPVEGKSQFPDFLDIVKRELNKVYYSDDLKNEGLI
IISTMDPIAQLAADKAVERKLGELRRSGSKTKDLQGALVSANPETGELVSVVGSGSEFTGFNRAVDAKRQVGSLLKPIIY
MTALESGRYNLASSVDDSPITVSLSDGTEWNPKNYDNRDHGYIPFTTALSQSYNHSAVRLGMEFGVDTFAKQLKRMGVKE
KIPPYPAALLGSVNLSPMDMLGVYQVFATGGFRTPIHSIRSVIDDRGSILQRTGLNTQRSIPPETNFLINYALQDVVKNG
TARRVQSLGSNLNLAGKTGTTNDYRDAWFAGYSGNYVSVVWVGRDDNKPIGLSGGSGALPVWVDYMNRLKLTPVALPEPE
GIEWLWLENNSGKLSNERCASARYLPVMSAYLPQEASSCAIGLYQQDRAREQMQWQNQQGDINKQRRREGLDIPDGEVID
NNSNDAEQDGDTPNRVDTWYDRAVEWF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=731, Percent_Identity=38.9876880984952, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI87082258, Length=243, Percent_Identity=39.917695473251, Blast_Score=172, Evalue=1e-43,
Organism=Escherichia coli, GI1788867, Length=563, Percent_Identity=28.7744227353464, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789601, Length=169, Percent_Identity=33.1360946745562, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 92002; Mature: 92002

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSNSKQPQSSKPAINTLPSQQRGVVFSSILLIVVIAGMVLLALYLVKLDRTITHKFEG
CCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KRWDIPAKVYSQPLELYQGANVDKDTMKKWLELLNYQSDKAYDRTGTYHKSGNTYFIHTR
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEEEEEC
GFTYSANDIDQEQVIKMTIAGDKIESVQSTVAAKTGIIRLEPVNIGGIYPDSNEDRMVVS
CEEECCCCCCHHHEEEEEECCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEEEE
LDQVPQPLIDALIATEDRGFYEHKGVSIRGIARAVINNFSGGSRQGGSTITQQLIKNFYL
HHHCCHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
NSDRTMKRKANEALMAVLLELHYSKDEILQAYLNEIYLGQNGKRSINGFGLASQFYFDKP
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCC
LNELRLDQQALLVGMAKGPSVYNPRRHPNDSKARRDVVLSNMLAVGSLSQEDYDKALKNS
HHHHHCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
LGVVDKPVEGKSQFPDFLDIVKRELNKVYYSDDLKNEGLIIISTMDPIAQLAADKAVERK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCEEEEECCCHHHHHHHHHHHHHH
LGELRRSGSKTKDLQGALVSANPETGELVSVVGSGSEFTGFNRAVDAKRQVGSLLKPIIY
HHHHHHCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
MTALESGRYNLASSVDDSPITVSLSDGTEWNPKNYDNRDHGYIPFTTALSQSYNHSAVRL
HHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHCCCCCHHEEE
GMEFGVDTFAKQLKRMGVKEKIPPYPAALLGSVNLSPMDMLGVYQVFATGGFRTPIHSIR
HHHHCHHHHHHHHHHCCCHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
SVIDDRGSILQRTGLNTQRSIPPETNFLINYALQDVVKNGTARRVQSLGSNLNLAGKTGT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCEECCCCCC
TNDYRDAWFAGYSGNYVSVVWVGRDDNKPIGLSGGSGALPVWVDYMNRLKLTPVALPEPE
CCCCCHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCEECEECCCCCC
GIEWLWLENNSGKLSNERCASARYLPVMSAYLPQEASSCAIGLYQQDRAREQMQWQNQQG
CCEEEEEECCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCC
DINKQRRREGLDIPDGEVIDNNSNDAEQDGDTPNRVDTWYDRAVEWF
CCCHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQKSNSKQPQSSKPAINTLPSQQRGVVFSSILLIVVIAGMVLLALYLVKLDRTITHKFEG
CCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KRWDIPAKVYSQPLELYQGANVDKDTMKKWLELLNYQSDKAYDRTGTYHKSGNTYFIHTR
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEEEEEC
GFTYSANDIDQEQVIKMTIAGDKIESVQSTVAAKTGIIRLEPVNIGGIYPDSNEDRMVVS
CEEECCCCCCHHHEEEEEECCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEEEE
LDQVPQPLIDALIATEDRGFYEHKGVSIRGIARAVINNFSGGSRQGGSTITQQLIKNFYL
HHHCCHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
NSDRTMKRKANEALMAVLLELHYSKDEILQAYLNEIYLGQNGKRSINGFGLASQFYFDKP
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCC
LNELRLDQQALLVGMAKGPSVYNPRRHPNDSKARRDVVLSNMLAVGSLSQEDYDKALKNS
HHHHHCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
LGVVDKPVEGKSQFPDFLDIVKRELNKVYYSDDLKNEGLIIISTMDPIAQLAADKAVERK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCEEEEECCCHHHHHHHHHHHHHH
LGELRRSGSKTKDLQGALVSANPETGELVSVVGSGSEFTGFNRAVDAKRQVGSLLKPIIY
HHHHHHCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
MTALESGRYNLASSVDDSPITVSLSDGTEWNPKNYDNRDHGYIPFTTALSQSYNHSAVRL
HHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHCCCCCHHEEE
GMEFGVDTFAKQLKRMGVKEKIPPYPAALLGSVNLSPMDMLGVYQVFATGGFRTPIHSIR
HHHHCHHHHHHHHHHCCCHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
SVIDDRGSILQRTGLNTQRSIPPETNFLINYALQDVVKNGTARRVQSLGSNLNLAGKTGT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCEECCCCCC
TNDYRDAWFAGYSGNYVSVVWVGRDDNKPIGLSGGSGALPVWVDYMNRLKLTPVALPEPE
CCCCCHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCEECEECCCCCC
GIEWLWLENNSGKLSNERCASARYLPVMSAYLPQEASSCAIGLYQQDRAREQMQWQNQQG
CCEEEEEECCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCC
DINKQRRREGLDIPDGEVIDNNSNDAEQDGDTPNRVDTWYDRAVEWF
CCCHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]