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The map label for this gene is lysC [H]

Identifier: 93006463

GI number: 93006463

Start: 2018934

End: 2020220

Strand: Direct

Name: lysC [H]

Synonym: Pcryo_1639

Alternate gene names: 93006463

Gene position: 2018934-2020220 (Clockwise)

Preceding gene: 93006462

Following gene: 93006464

Centisome position: 65.98

GC content: 45.77

Gene sequence:

>1287_bases
ATGGCGTTAATAGTACAAAAATATGGCGGCACCTCGATGGGCAGTATCGACCGCATCAAAAACGTCGCCAAACGAGTCAA
ACGCTGGCATGACAATGGTCATCAAGTGGTCGTTGTCGTGTCTGCCATGAGCGGTGAAACCAACCGTTTGATTGATTTAG
CACGCCAAATCAGTAGCCAGCCCGATCCTCGCGAATATGACCAAATGGTCTCAACCGGCGAGCAGGTGTCTATCTCACTG
CTTGCCATGGCGATTAAAGAGTTGGGTATCGGTGCACGTTCATTTACCGGTCGTCAAGTCGCTATTAAAACCGATAATGC
TCACAATAAAGCGCGTATTGAAAGTATCGATGATAAAAACATTCGTGAGCAGCTAGATGCTGGTAACGTCGTTATTGTCG
CAGGCTTCCAAGGTATCGACGAAGAAGGCAATGCGACGACGTTAGGGCGCGGCGGATCTGATACGACAGGCGTGGCGATT
GCCGCGGCTTTAGGCGCGGATGAATGCCAAATCTATACTGATGTCGATGGCGTCTATACCACTGACCCACGCGTCACCTC
AAAAGCCAAAAAACTTGAAAAGATTACTTTTGAAGAAATGCTTGAGATGGCAAGTCTTGGCTCTAAGATTTTACAGATTC
GCTCAGTAGAATTTGCTGGCAAATACGGCGTACCGCTGCGCGTATTATCTAGCTTTGATGAAAACAACGATGGTAGCTTC
GACCAAGAATTTCAAGACAATGTCGGCACCCTAATTACGATAGACGAAGGAGACAATATGGAACAGGCAATCATCTCAGG
TATCGCTTTTAATCGAGACGAAGCAAAAATCGTCGTACGCGGTGTCCCTGATCACCCTGGCATTGCTTCTGCTATCCTTA
GCCCTATTGGCCGCGCCAATATCGAAATTGATATGATCGTGCAAAACCTATCTACCAACGGTATGACTGATTTTACTTTT
ACCGTTAATCGTACGGACATGGAAAAAACCATGAAAGTACTAGAAAGCGAAGTCAAAGATGAAATCGGTGCTAAAGAAAT
ATTGGCCAATGATGAAGTGGTTAAAGTCTCATTGGTCGGCGTCGGCATGCGCTCGCACGCAGGTGTTGCTAGTCTTATGT
TCCAAACCCTTGCGGAAAACAACATCAATATTCAAATGATATCGACCAGTGAAATCAAGGTATCTGTTCTTATCCAAGAG
CAGTATCTGGAAAAAGCGGTAAGATCACTGCATACGGCCTTTGGTCTTGATCGTGAAGATGGTGAAAGCAAAATCGCTGG
TTTATAA

Upstream 100 bases:

>100_bases
TCGTTATTAAAATCAATCTAGCACTTAATTTAGACAGTGCCATGACGAATTGATGTCTCTCAATAACAGATAACCATTGG
TGAATAATAGGTAGATTTTT

Downstream 100 bases:

>100_bases
GAATAAGCATATAAATACTTTACTAAAGCCGTATAAAAATAATAGGCTTTAGTATTTATTCAATAAACTGCGATTTATTA
TTTTCAATAATACTTAATTA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MALIVQKYGGTSMGSIDRIKNVAKRVKRWHDNGHQVVVVVSAMSGETNRLIDLARQISSQPDPREYDQMVSTGEQVSISL
LAMAIKELGIGARSFTGRQVAIKTDNAHNKARIESIDDKNIREQLDAGNVVIVAGFQGIDEEGNATTLGRGGSDTTGVAI
AAALGADECQIYTDVDGVYTTDPRVTSKAKKLEKITFEEMLEMASLGSKILQIRSVEFAGKYGVPLRVLSSFDENNDGSF
DQEFQDNVGTLITIDEGDNMEQAIISGIAFNRDEAKIVVRGVPDHPGIASAILSPIGRANIEIDMIVQNLSTNGMTDFTF
TVNRTDMEKTMKVLESEVKDEIGAKEILANDEVVKVSLVGVGMRSHAGVASLMFQTLAENNINIQMISTSEIKVSVLIQE
QYLEKAVRSLHTAFGLDREDGESKIAGL

Sequences:

>Translated_428_residues
MALIVQKYGGTSMGSIDRIKNVAKRVKRWHDNGHQVVVVVSAMSGETNRLIDLARQISSQPDPREYDQMVSTGEQVSISL
LAMAIKELGIGARSFTGRQVAIKTDNAHNKARIESIDDKNIREQLDAGNVVIVAGFQGIDEEGNATTLGRGGSDTTGVAI
AAALGADECQIYTDVDGVYTTDPRVTSKAKKLEKITFEEMLEMASLGSKILQIRSVEFAGKYGVPLRVLSSFDENNDGSF
DQEFQDNVGTLITIDEGDNMEQAIISGIAFNRDEAKIVVRGVPDHPGIASAILSPIGRANIEIDMIVQNLSTNGMTDFTF
TVNRTDMEKTMKVLESEVKDEIGAKEILANDEVVKVSLVGVGMRSHAGVASLMFQTLAENNINIQMISTSEIKVSVLIQE
QYLEKAVRSLHTAFGLDREDGESKIAGL
>Mature_427_residues
ALIVQKYGGTSMGSIDRIKNVAKRVKRWHDNGHQVVVVVSAMSGETNRLIDLARQISSQPDPREYDQMVSTGEQVSISLL
AMAIKELGIGARSFTGRQVAIKTDNAHNKARIESIDDKNIREQLDAGNVVIVAGFQGIDEEGNATTLGRGGSDTTGVAIA
AALGADECQIYTDVDGVYTTDPRVTSKAKKLEKITFEEMLEMASLGSKILQIRSVEFAGKYGVPLRVLSSFDENNDGSFD
QEFQDNVGTLITIDEGDNMEQAIISGIAFNRDEAKIVVRGVPDHPGIASAILSPIGRANIEIDMIVQNLSTNGMTDFTFT
VNRTDMEKTMKVLESEVKDEIGAKEILANDEVVKVSLVGVGMRSHAGVASLMFQTLAENNINIQMISTSEIKVSVLIQEQ
YLEKAVRSLHTAFGLDREDGESKIAGL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=357, Percent_Identity=27.4509803921569, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790455, Length=465, Percent_Identity=24.9462365591398, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790376, Length=364, Percent_Identity=22.5274725274725, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320893, Length=503, Percent_Identity=23.8568588469185, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 46428; Mature: 46297

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVQKYGGTSMGSIDRIKNVAKRVKRWHDNGHQVVVVVSAMSGETNRLIDLARQISSQ
CEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHCCC
PDPREYDQMVSTGEQVSISLLAMAIKELGIGARSFTGRQVAIKTDNAHNKARIESIDDKN
CCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHCCCCCCHH
IREQLDAGNVVIVAGFQGIDEEGNATTLGRGGSDTTGVAIAAALGADECQIYTDVDGVYT
HHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCCCHHEEEEECCCCCEEEEECCCCEEE
TDPRVTSKAKKLEKITFEEMLEMASLGSKILQIRSVEFAGKYGVPLRVLSSFDENNDGSF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHCCCCCCCCC
DQEFQDNVGTLITIDEGDNMEQAIISGIAFNRDEAKIVVRGVPDHPGIASAILSPIGRAN
CHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCCCC
IEIDMIVQNLSTNGMTDFTFTVNRTDMEKTMKVLESEVKDEIGAKEILANDEVVKVSLVG
CEEEEEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEEE
VGMRSHAGVASLMFQTLAENNINIQMISTSEIKVSVLIQEQYLEKAVRSLHTAFGLDRED
ECCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCC
GESKIAGL
CCHHCCCC
>Mature Secondary Structure 
ALIVQKYGGTSMGSIDRIKNVAKRVKRWHDNGHQVVVVVSAMSGETNRLIDLARQISSQ
EEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHCCC
PDPREYDQMVSTGEQVSISLLAMAIKELGIGARSFTGRQVAIKTDNAHNKARIESIDDKN
CCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHCCCCCCHH
IREQLDAGNVVIVAGFQGIDEEGNATTLGRGGSDTTGVAIAAALGADECQIYTDVDGVYT
HHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCCCHHEEEEECCCCCEEEEECCCCEEE
TDPRVTSKAKKLEKITFEEMLEMASLGSKILQIRSVEFAGKYGVPLRVLSSFDENNDGSF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHCCCCCCCCC
DQEFQDNVGTLITIDEGDNMEQAIISGIAFNRDEAKIVVRGVPDHPGIASAILSPIGRAN
CHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCCCC
IEIDMIVQNLSTNGMTDFTFTVNRTDMEKTMKVLESEVKDEIGAKEILANDEVVKVSLVG
CEEEEEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEEE
VGMRSHAGVASLMFQTLAENNINIQMISTSEIKVSVLIQEQYLEKAVRSLHTAFGLDRED
ECCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCC
GESKIAGL
CCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]