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The map label for this gene is opuE [H]

Identifier: 93006400

GI number: 93006400

Start: 1933223

End: 1934722

Strand: Direct

Name: opuE [H]

Synonym: Pcryo_1576

Alternate gene names: 93006400

Gene position: 1933223-1934722 (Clockwise)

Preceding gene: 93006399

Following gene: 93006406

Centisome position: 63.18

GC content: 43.67

Gene sequence:

>1500_bases
ATGAACGGAGTTTCTAGTGGCGTGCTGATATCACTTGGCGCCTATTTCTTAGTGATGATCGGGATTGGGGTTTATGCTTA
TTTTAAGCAAGCCAATGATACCGAAGGCTATATGCTTGGTGGTCGTAGTCTCGGTCCTGCGGTAACCGCTTTATCCGCTG
GCGCATCAGACATGTCCGGTTGGTTATTACTTGGTTTACCGGGTTATATGTATGCCGATGGCGTCGTGAGTATCTGGATT
GCCCTCGGTTTGACATTGGGTGCATTTTTAAACTATATCATCGTGGCACCGCGCTTACGTGTTTACACTGAAATTGCAGA
TAATGCCATCACATTACCTGACTACTTTGCAAATCGATTCGAAGATAAGTCGCATATGCTGCGTGTTATCTCAGCAGTTG
TAATTATTTTATTCTTTACTGTTTATACAGCAGCTAGCTTAGTTGGTGGTGGTAAACTGTTTGAAAGCTCACTCAACTTA
TCTTATACCACAGGTTTGTGGGTGACTGCTGGTGTGGTTGTCGCCTATACTCTATTTGGCGGCTTTTTAGCAGTGTCAAT
GACTGACTTTGTACAGGGCATTATCATGCTATTTGCGATGGTTATCGTACCTGTTGTAGCCTTTACTGATCTTGGCGGAA
TTTCAGCCACAACTGAGGCGGTCAGGAACATTGATCCAAGCTTACTTAATGTCACCAGTGGTATGACGATCCTTGGTATT
GTCTCGTTAATGGCTTGGGGTTTGGGGTACTTTGGGCAGCCGCATATTATTGTACGCTTTATGGCTATTCGCTCAGTCAA
AGATATACCAACGGCACGTAACATTGGTATGAGCTGGATGATTGTGAGTCTAATCGGTGCATTAATGACTGGTTTTGCAG
GTCGTGCTTATGTACAGAAAACAGCAATGACTTTAGATGATCCTGAAACTATCTTCTTAGTATTCACCCAGTTCTTATTC
ACGCCATTAGTTTCAGGCTTCTTGTTAGCCGCTATTTTAGCCGCGATCATGAGTACAATCTCATCACAGCTATTGGTCGT
ATCAAGCTCACTAACCAAAGATGTGTACAAGCTGTTTTTTGATAGAGATGCACCAGAAGCCCGCCAAGTAATGGTCGGTC
GTCTTTCAGTTATCGTGGTCGCCGTCGTTGCTATTTTGCTGGCTTCTAATCCTGAAAGCTCTGTATTAAGTTTGGTCTCT
AACGCTTGGGCAGGATTTGGTGCGGCATTTGGACCGTTGGTTATTTTCAGTCTTATCTGGCGCGGTATGAACCGAAACGG
TGCGGTTGCTGGTATGGTCGTTGGTGCATTAACTGTCATCTTATGGATCTATGGTCCTTTCCAGATGAATGGTATGGCAC
TAAATAGCTGGTTATACGCTATCGTTCCAGGCTTTATCCTAAGTACTATTGCCATCTTTGCTGTAAGTATTATGACAGGT
GGTCCAAAAGCCTCAGTCTCAGCGAAGTTTAAAGAAATGGAAATGAACTTAGATCAATAA

Upstream 100 bases:

>100_bases
TTTTTGTCTTTAGATATTGTTTCTAAACATCGTCTTGCAAGACTTAATTTTTCTCGTTAAAAATAATGACAGCCAACAAC
GTTGGTTAAGGACTATTTAT

Downstream 100 bases:

>100_bases
GCACTTTTTAATTACTGCTGTTAAATTAAAAAACCTCGCTACTGAACTGACCCCCAAATGTTGGACGGTTTAGTTTATAT
CAAGGACTGAGTTCTGTACT

Product: Sodium/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNGVSSGVLISLGAYFLVMIGIGVYAYFKQANDTEGYMLGGRSLGPAVTALSAGASDMSGWLLLGLPGYMYADGVVSIWI
ALGLTLGAFLNYIIVAPRLRVYTEIADNAITLPDYFANRFEDKSHMLRVISAVVIILFFTVYTAASLVGGGKLFESSLNL
SYTTGLWVTAGVVVAYTLFGGFLAVSMTDFVQGIIMLFAMVIVPVVAFTDLGGISATTEAVRNIDPSLLNVTSGMTILGI
VSLMAWGLGYFGQPHIIVRFMAIRSVKDIPTARNIGMSWMIVSLIGALMTGFAGRAYVQKTAMTLDDPETIFLVFTQFLF
TPLVSGFLLAAILAAIMSTISSQLLVVSSSLTKDVYKLFFDRDAPEARQVMVGRLSVIVVAVVAILLASNPESSVLSLVS
NAWAGFGAAFGPLVIFSLIWRGMNRNGAVAGMVVGALTVILWIYGPFQMNGMALNSWLYAIVPGFILSTIAIFAVSIMTG
GPKASVSAKFKEMEMNLDQ

Sequences:

>Translated_499_residues
MNGVSSGVLISLGAYFLVMIGIGVYAYFKQANDTEGYMLGGRSLGPAVTALSAGASDMSGWLLLGLPGYMYADGVVSIWI
ALGLTLGAFLNYIIVAPRLRVYTEIADNAITLPDYFANRFEDKSHMLRVISAVVIILFFTVYTAASLVGGGKLFESSLNL
SYTTGLWVTAGVVVAYTLFGGFLAVSMTDFVQGIIMLFAMVIVPVVAFTDLGGISATTEAVRNIDPSLLNVTSGMTILGI
VSLMAWGLGYFGQPHIIVRFMAIRSVKDIPTARNIGMSWMIVSLIGALMTGFAGRAYVQKTAMTLDDPETIFLVFTQFLF
TPLVSGFLLAAILAAIMSTISSQLLVVSSSLTKDVYKLFFDRDAPEARQVMVGRLSVIVVAVVAILLASNPESSVLSLVS
NAWAGFGAAFGPLVIFSLIWRGMNRNGAVAGMVVGALTVILWIYGPFQMNGMALNSWLYAIVPGFILSTIAIFAVSIMTG
GPKASVSAKFKEMEMNLDQ
>Mature_499_residues
MNGVSSGVLISLGAYFLVMIGIGVYAYFKQANDTEGYMLGGRSLGPAVTALSAGASDMSGWLLLGLPGYMYADGVVSIWI
ALGLTLGAFLNYIIVAPRLRVYTEIADNAITLPDYFANRFEDKSHMLRVISAVVIILFFTVYTAASLVGGGKLFESSLNL
SYTTGLWVTAGVVVAYTLFGGFLAVSMTDFVQGIIMLFAMVIVPVVAFTDLGGISATTEAVRNIDPSLLNVTSGMTILGI
VSLMAWGLGYFGQPHIIVRFMAIRSVKDIPTARNIGMSWMIVSLIGALMTGFAGRAYVQKTAMTLDDPETIFLVFTQFLF
TPLVSGFLLAAILAAIMSTISSQLLVVSSSLTKDVYKLFFDRDAPEARQVMVGRLSVIVVAVVAILLASNPESSVLSLVS
NAWAGFGAAFGPLVIFSLIWRGMNRNGAVAGMVVGALTVILWIYGPFQMNGMALNSWLYAIVPGFILSTIAIFAVSIMTG
GPKASVSAKFKEMEMNLDQ

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=490, Percent_Identity=53.469387755102, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI14140236, Length=470, Percent_Identity=22.1276595744681, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI110835708, Length=489, Percent_Identity=22.9038854805726, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1787251, Length=497, Percent_Identity=50.3018108651911, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI87082237, Length=448, Percent_Identity=29.6875, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1790503, Length=445, Percent_Identity=23.8202247191011, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24651739, Length=486, Percent_Identity=26.9547325102881, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648033, Length=393, Percent_Identity=23.1552162849873, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356865, Length=393, Percent_Identity=23.1552162849873, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24645928, Length=377, Percent_Identity=25.4641909814324, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281362918, Length=235, Percent_Identity=31.063829787234, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53250; Mature: 53250

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGVSSGVLISLGAYFLVMIGIGVYAYFKQANDTEGYMLGGRSLGPAVTALSAGASDMSG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHCCCCCCCC
WLLLGLPGYMYADGVVSIWIALGLTLGAFLNYIIVAPRLRVYTEIADNAITLPDYFANRF
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHH
EDKSHMLRVISAVVIILFFTVYTAASLVGGGKLFESSLNLSYTTGLWVTAGVVVAYTLFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEECHHHHHHHHHHHHHHHH
GFLAVSMTDFVQGIIMLFAMVIVPVVAFTDLGGISATTEAVRNIDPSLLNVTSGMTILGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHH
VSLMAWGLGYFGQPHIIVRFMAIRSVKDIPTARNIGMSWMIVSLIGALMTGFAGRAYVQK
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHH
TAMTLDDPETIFLVFTQFLFTPLVSGFLLAAILAAIMSTISSQLLVVSSSLTKDVYKLFF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRDAPEARQVMVGRLSVIVVAVVAILLASNPESSVLSLVSNAWAGFGAAFGPLVIFSLIW
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RGMNRNGAVAGMVVGALTVILWIYGPFQMNGMALNSWLYAIVPGFILSTIAIFAVSIMTG
CCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPKASVSAKFKEMEMNLDQ
CCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNGVSSGVLISLGAYFLVMIGIGVYAYFKQANDTEGYMLGGRSLGPAVTALSAGASDMSG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHCCCCCCCC
WLLLGLPGYMYADGVVSIWIALGLTLGAFLNYIIVAPRLRVYTEIADNAITLPDYFANRF
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHH
EDKSHMLRVISAVVIILFFTVYTAASLVGGGKLFESSLNLSYTTGLWVTAGVVVAYTLFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEECHHHHHHHHHHHHHHHH
GFLAVSMTDFVQGIIMLFAMVIVPVVAFTDLGGISATTEAVRNIDPSLLNVTSGMTILGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHH
VSLMAWGLGYFGQPHIIVRFMAIRSVKDIPTARNIGMSWMIVSLIGALMTGFAGRAYVQK
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHH
TAMTLDDPETIFLVFTQFLFTPLVSGFLLAAILAAIMSTISSQLLVVSSSLTKDVYKLFF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRDAPEARQVMVGRLSVIVVAVVAILLASNPESSVLSLVSNAWAGFGAAFGPLVIFSLIW
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RGMNRNGAVAGMVVGALTVILWIYGPFQMNGMALNSWLYAIVPGFILSTIAIFAVSIMTG
CCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPKASVSAKFKEMEMNLDQ
CCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]