Click here to switch to the map view.

The map label for this gene is yomI [H]

Identifier: 93005223

GI number: 93005223

Start: 477947

End: 478864

Strand: Reverse

Name: yomI [H]

Synonym: Pcryo_0393

Alternate gene names: 93005223

Gene position: 478864-477947 (Counterclockwise)

Preceding gene: 93005224

Following gene: 93005210

Centisome position: 15.65

GC content: 43.36

Gene sequence:

>918_bases
ATGAAATATTGCAGACAGGATAACCGCTTTATTGCGCAAACCAATGCGTCCATAAGCAAGGCAATTTGCTACTACTCGAT
AAAAAGTGATAACCCTATGATAAATGTTGCACCTTTCATAACCCGCTGCTTGTCTCCCGTTTTACTCACCGCCGTTGCCC
TATCTAGCCTACCTATCGCTGCTCAAGCAGGCAATATGTATATCTATAAAGACAAAGGCGGACAAGTACTTCTCACCAAC
GTCAATCCCAGTGGTAATTTTGATAAATTTACTAAAAAAGTAAAAGTCACTTATTATAAAGATTCTAGTCTGTATGATGG
TGGTAGCAGCAGTAATAGCAATGACTATGGGAGCAGCTCTGCAAGCAGCAGCGGTACTCGTAACTCTTATGATAGCTATA
TTCTGGCTTCAGCGCAGCGCCACGGTGTCGATCCAGGACTTATGAAAGCCATGATGCATACTGAGTCAGCCTTTAATCCC
AATGCTCGCTCACCAGTCGGTGCGCAGGGTTTAATGCAGCTGATGCCAGCTACAGCACGTCGATTTAAAGTGAGCAATCC
TTGGAATCCTGCTGATAATATTGAAGGTTCTGCCAAATATATCGCTTGGCTGATGCGCCGCTTTAACAACAACGTCGAGT
TTGCGGTCGCGGGTTATAATGCAGGTGAAGGTAACGTCGATAAATATAATGGCATTCCACCGTTTAAAGAAACGCGTAAC
TATGTCAAAAGTGTGATGAGCCGATATCATAGCCTGTATAAAAACGATTCAGGGCTATCTGGTAATACGATGAATGCTAA
TAATAGCAATACTGCCAATACCAGTAATGGCGGTGGCATGCAAAACGTTAGTTATGGCACGAGTAGCAACAGTAATAGCG
CAAGCTATGCCAACTCTGCTTATTTGGCATTGCGTTAA

Upstream 100 bases:

>100_bases
AGTTATTATATGTTATAGTAAGCAACTATTATTTTTTGTATCTATTGCAATTGGTTTTGTTAACCGTGGTTCTGCGTTGT
AGTACTTAAGCTTAGTGACT

Downstream 100 bases:

>100_bases
TCCAAAATAGAAATCACAGACATAAAAAAACCCGTTAAATTGAACTGACCCCCTAAACTTGGACAGTTTAATTAAATCAA
GGACTGAGTTCTGTATTGTA

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKYCRQDNRFIAQTNASISKAICYYSIKSDNPMINVAPFITRCLSPVLLTAVALSSLPIAAQAGNMYIYKDKGGQVLLTN
VNPSGNFDKFTKKVKVTYYKDSSLYDGGSSSNSNDYGSSSASSSGTRNSYDSYILASAQRHGVDPGLMKAMMHTESAFNP
NARSPVGAQGLMQLMPATARRFKVSNPWNPADNIEGSAKYIAWLMRRFNNNVEFAVAGYNAGEGNVDKYNGIPPFKETRN
YVKSVMSRYHSLYKNDSGLSGNTMNANNSNTANTSNGGGMQNVSYGTSSNSNSASYANSAYLALR

Sequences:

>Translated_305_residues
MKYCRQDNRFIAQTNASISKAICYYSIKSDNPMINVAPFITRCLSPVLLTAVALSSLPIAAQAGNMYIYKDKGGQVLLTN
VNPSGNFDKFTKKVKVTYYKDSSLYDGGSSSNSNDYGSSSASSSGTRNSYDSYILASAQRHGVDPGLMKAMMHTESAFNP
NARSPVGAQGLMQLMPATARRFKVSNPWNPADNIEGSAKYIAWLMRRFNNNVEFAVAGYNAGEGNVDKYNGIPPFKETRN
YVKSVMSRYHSLYKNDSGLSGNTMNANNSNTANTSNGGGMQNVSYGTSSNSNSASYANSAYLALR
>Mature_305_residues
MKYCRQDNRFIAQTNASISKAICYYSIKSDNPMINVAPFITRCLSPVLLTAVALSSLPIAAQAGNMYIYKDKGGQVLLTN
VNPSGNFDKFTKKVKVTYYKDSSLYDGGSSSNSNDYGSSSASSSGTRNSYDSYILASAQRHGVDPGLMKAMMHTESAFNP
NARSPVGAQGLMQLMPATARRFKVSNPWNPADNIEGSAKYIAWLMRRFNNNVEFAVAGYNAGEGNVDKYNGIPPFKETRN
YVKSVMSRYHSLYKNDSGLSGNTMNANNSNTANTSNGGGMQNVSYGTSSNSNSASYANSAYLALR

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=142, Percent_Identity=36.6197183098592, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR008258
- InterPro:   IPR016047
- InterPro:   IPR010090
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01551 Peptidase_M23; PF10145 PhageMin_Tail; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 33089; Mature: 33089

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYCRQDNRFIAQTNASISKAICYYSIKSDNPMINVAPFITRCLSPVLLTAVALSSLPIA
CCCCCCCCCEEEECCCCHHEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCE
AQAGNMYIYKDKGGQVLLTNVNPSGNFDKFTKKVKVTYYKDSSLYDGGSSSNSNDYGSSS
EECCCEEEEECCCCEEEEEECCCCCCHHHHHEEEEEEEEECCCCCCCCCCCCCCCCCCCC
ASSSGTRNSYDSYILASAQRHGVDPGLMKAMMHTESAFNPNARSPVGAQGLMQLMPATAR
CCCCCCCCCCCCCEEEHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
RFKVSNPWNPADNIEGSAKYIAWLMRRFNNNVEFAVAGYNAGEGNVDKYNGIPPFKETRN
HEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHH
YVKSVMSRYHSLYKNDSGLSGNTMNANNSNTANTSNGGGMQNVSYGTSSNSNSASYANSA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
YLALR
EEEEC
>Mature Secondary Structure
MKYCRQDNRFIAQTNASISKAICYYSIKSDNPMINVAPFITRCLSPVLLTAVALSSLPIA
CCCCCCCCCEEEECCCCHHEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCE
AQAGNMYIYKDKGGQVLLTNVNPSGNFDKFTKKVKVTYYKDSSLYDGGSSSNSNDYGSSS
EECCCEEEEECCCCEEEEEECCCCCCHHHHHEEEEEEEEECCCCCCCCCCCCCCCCCCCC
ASSSGTRNSYDSYILASAQRHGVDPGLMKAMMHTESAFNPNARSPVGAQGLMQLMPATAR
CCCCCCCCCCCCCEEEHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
RFKVSNPWNPADNIEGSAKYIAWLMRRFNNNVEFAVAGYNAGEGNVDKYNGIPPFKETRN
HEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHH
YVKSVMSRYHSLYKNDSGLSGNTMNANNSNTANTSNGGGMQNVSYGTSSNSNSASYANSA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
YLALR
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]