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The map label for this gene is cysM [H]

Identifier: 93005210

GI number: 93005210

Start: 462014

End: 462970

Strand: Reverse

Name: cysM [H]

Synonym: Pcryo_0380

Alternate gene names: 93005210

Gene position: 462970-462014 (Counterclockwise)

Preceding gene: 93005223

Following gene: 93005209

Centisome position: 15.13

GC content: 46.5

Gene sequence:

>957_bases
ATGTCCGCTACAGCTCAGTCAAACACCAATTTTATTACTCAAATTACTGACCTTGCTGATTGCGTCGGTCAAACGCCGTT
AGTCAAATTGCAGCGCTTACCTGAACAAGAGCAGATTGACAATGGTGCTATCATGCTTGCCAAGCTTGAAGGTAATAATC
CTGCTGGTTCTGTCAAAGACCGCCCTGCTTTTAATATGATTTATCAAGCGGAGCTACGCGGTGATATTAAGCCAGGTGAC
ATGTTGATTGAGGCAACCAGTGGCAATACTGGTATTGCACTAGCCATGGTAGCGGCGATGCGTGGCTATCCGATTACCTT
GTTTATGCCAAGCAACTCAACCCAAGAGCGTAAAGACGCCATGACGGCTTATGGTGCGACGCTGATTGAGGTAGAAGAAG
GGATAGAAGCCGCGCGCGACATGGCGCTACAAATGCAAACGGACGGTAAAGGTATTGTCTTAGATCAGTTTAATAACCCT
GATAATAAACAGGCGCATTATCTGACTACAGGTCCTGAGCTGTGGGCGCAAACAGACGGTAAAATTACCCATTTTATCAG
CTCTATGGGTACCACAGGCACCATTACCGGCGTGGCACAATATTTAAAAGAGCAAAACCCTGATATACAAATCATCGGTT
TACAGCCTGATGAGGATGCAGCTATCGCTGGTATCCGCCGCTGGCCTGCCGCTTATATGCCAGGGATTTTTGATGCCGAC
TTAGTCGATGAGATTATGGATATTGATCAGCGCGTCGCTGAGGTTTATATGCGTAAGCTTGCTAAAACCGAAGGGATTTT
TGCGGGTGTCTCATCAGGTGCAGCAGCATGGGCGGCTACACAAGTTGCTAAGGCAAATCCTGATGCCGTCATTGCCTTTA
TCGTCTGTGACCGTGGGGATCGTTATTTATCGACCGGCTTATATAATGTTGATGATAGCTTAGATAATGTAAACTAA

Upstream 100 bases:

>100_bases
ATAATAAAAACGCTGCTGTTACCTCAAAGTATTGCCGAATATATAGCACTTTACTATAGCGTTAAACTACTTTAAAAATA
GATACTATTAAGGATACTTT

Downstream 100 bases:

>100_bases
GATAATTTATAAATCGCTGACAATGCCGTTTATGCGGTCAGTGCGTTTAATGCAGTGTAGTAAGACAATAATCAGGTAGA
AACCTCTATGTCACAAGCTT

Product: cysteine synthase B

Products: NA

Alternate protein names: CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSATAQSNTNFITQITDLADCVGQTPLVKLQRLPEQEQIDNGAIMLAKLEGNNPAGSVKDRPAFNMIYQAELRGDIKPGD
MLIEATSGNTGIALAMVAAMRGYPITLFMPSNSTQERKDAMTAYGATLIEVEEGIEAARDMALQMQTDGKGIVLDQFNNP
DNKQAHYLTTGPELWAQTDGKITHFISSMGTTGTITGVAQYLKEQNPDIQIIGLQPDEDAAIAGIRRWPAAYMPGIFDAD
LVDEIMDIDQRVAEVYMRKLAKTEGIFAGVSSGAAAWAATQVAKANPDAVIAFIVCDRGDRYLSTGLYNVDDSLDNVN

Sequences:

>Translated_318_residues
MSATAQSNTNFITQITDLADCVGQTPLVKLQRLPEQEQIDNGAIMLAKLEGNNPAGSVKDRPAFNMIYQAELRGDIKPGD
MLIEATSGNTGIALAMVAAMRGYPITLFMPSNSTQERKDAMTAYGATLIEVEEGIEAARDMALQMQTDGKGIVLDQFNNP
DNKQAHYLTTGPELWAQTDGKITHFISSMGTTGTITGVAQYLKEQNPDIQIIGLQPDEDAAIAGIRRWPAAYMPGIFDAD
LVDEIMDIDQRVAEVYMRKLAKTEGIFAGVSSGAAAWAATQVAKANPDAVIAFIVCDRGDRYLSTGLYNVDDSLDNVN
>Mature_317_residues
SATAQSNTNFITQITDLADCVGQTPLVKLQRLPEQEQIDNGAIMLAKLEGNNPAGSVKDRPAFNMIYQAELRGDIKPGDM
LIEATSGNTGIALAMVAAMRGYPITLFMPSNSTQERKDAMTAYGATLIEVEEGIEAARDMALQMQTDGKGIVLDQFNNPD
NKQAHYLTTGPELWAQTDGKITHFISSMGTTGTITGVAQYLKEQNPDIQIIGLQPDEDAAIAGIRRWPAAYMPGIFDADL
VDEIMDIDQRVAEVYMRKLAKTEGIFAGVSSGAAAWAATQVAKANPDAVIAFIVCDRGDRYLSTGLYNVDDSLDNVN

Specific function: Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=327, Percent_Identity=31.8042813455657, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI295821200, Length=327, Percent_Identity=31.8042813455657, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI4557415, Length=327, Percent_Identity=31.8042813455657, Blast_Score=159, Evalue=3e-39,
Organism=Escherichia coli, GI2367138, Length=296, Percent_Identity=63.8513513513513, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1788754, Length=309, Percent_Identity=41.4239482200647, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17535051, Length=321, Percent_Identity=40.1869158878505, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17562970, Length=319, Percent_Identity=39.4984326018809, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI32566674, Length=304, Percent_Identity=39.8026315789474, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI115535073, Length=304, Percent_Identity=39.8026315789474, Blast_Score=183, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI17561720, Length=310, Percent_Identity=38.7096774193548, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI32566672, Length=224, Percent_Identity=37.5, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=34.384858044164, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI25147552, Length=285, Percent_Identity=32.6315789473684, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321594, Length=319, Percent_Identity=34.4827586206897, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321449, Length=329, Percent_Identity=33.7386018237082, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643623, Length=212, Percent_Identity=42.4528301886792, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI20129101, Length=212, Percent_Identity=42.4528301886792, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005858
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34345; Mature: 34214

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATAQSNTNFITQITDLADCVGQTPLVKLQRLPEQEQIDNGAIMLAKLEGNNPAGSVKD
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCEEEEEEEECCCCCCCCCC
RPAFNMIYQAELRGDIKPGDMLIEATSGNTGIALAMVAAMRGYPITLFMPSNSTQERKDA
CCCHHEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHH
MTAYGATLIEVEEGIEAARDMALQMQTDGKGIVLDQFNNPDNKQAHYLTTGPELWAQTDG
HHHHCCEEEEHHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCCEEEEECCHHHHHCCCC
KITHFISSMGTTGTITGVAQYLKEQNPDIQIIGLQPDEDAAIAGIRRWPAAYMPGIFDAD
HHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCHHHCCCCCCHH
LVDEIMDIDQRVAEVYMRKLAKTEGIFAGVSSGAAAWAATQVAKANPDAVIAFIVCDRGD
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCC
RYLSTGLYNVDDSLDNVN
CEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SATAQSNTNFITQITDLADCVGQTPLVKLQRLPEQEQIDNGAIMLAKLEGNNPAGSVKD
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCEEEEEEEECCCCCCCCCC
RPAFNMIYQAELRGDIKPGDMLIEATSGNTGIALAMVAAMRGYPITLFMPSNSTQERKDA
CCCHHEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHH
MTAYGATLIEVEEGIEAARDMALQMQTDGKGIVLDQFNNPDNKQAHYLTTGPELWAQTDG
HHHHCCEEEEHHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCCEEEEECCHHHHHCCCC
KITHFISSMGTTGTITGVAQYLKEQNPDIQIIGLQPDEDAAIAGIRRWPAAYMPGIFDAD
HHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCHHHCCCCCCHH
LVDEIMDIDQRVAEVYMRKLAKTEGIFAGVSSGAAAWAATQVAKANPDAVIAFIVCDRGD
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCC
RYLSTGLYNVDDSLDNVN
CEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503 [H]