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The map label for this gene is lepA [H]

Identifier: 93005194

GI number: 93005194

Start: 442105

End: 443901

Strand: Direct

Name: lepA [H]

Synonym: Pcryo_0364

Alternate gene names: 93005194

Gene position: 442105-443901 (Clockwise)

Preceding gene: 93005193

Following gene: 93005195

Centisome position: 14.45

GC content: 45.41

Gene sequence:

>1797_bases
GTGACTGCATTAGCCAATATTCGTAACTTTTCTATTATTGCCCACATTGATCATGGTAAGTCGACCCTTGCCGATCGTTT
TATTCAAATGTGTGGCGCGCTGCAAGATCGCGAGATGCAGGCGCAAGTCCTCGATTCGATGGATATTGAGCGTGAGCGTG
GTATCACGATCAAAGCGCAGTCGGTGACGCTGTATTATGACCATCCAAATGGTGAGCGCTATCAGCTCAACTTTATTGAT
ACGCCTGGTCACGTGGATTTCTCTTATGAAGTTTCACGTTCCTTAGCGGCTTGTGAAGGCGCGCTATTGGTGGTTGATGC
GGCACAAGGTGTTGAAGCACAGTCGGTTGCTAACTGTTATACCGCAGTAGATCAAGGTCTTGAGGTCATGGCAGTTTTAA
ATAAAATTGACTTGCCACAAGTTGAGCCTGAGCGCGTCATTCAAGAGATTGAAGACATCATTGGTATTGATGCCGTTGAT
GCGCCACGCGTGTCGGCAAAATCTGGTCTCGGCGTTGATAAACTGCTTGAGGCGTTGGTTGAATTTATTCCTGCGCCAAC
GGGTGATCGTGATGCACCGCTGCAAGCGTTGATTATTGACTCATGGTTTGATAATTATTTAGGCGTGGTTTCATTAGTCC
GTGTACGCCAAGGTACGATAAAAAAAGGTGATAAACTTTATATTAAATCGACCAAAGATGCGCATTTAGTCGGATCAATT
GGTGTCTTTACCCCGAAGCCGTTAGATACGGGTATCTTAGAAGCGGGTGAAGTGGGCTTTATTATTGCGGGTATTAAAGA
TATTGCAGGCGCGCCAGTTGGTGATACGATTACGCATGCCAGTACGCCTGACGTCGATCGTATTCCTGGATTTAAACAGA
TTACGCCGCAAGTGTATGCTGGTATGTTTCCTGTAGAATCTACCGACTTTGAAAAGTTCCGTGAAGCACTACAAAAACTG
CAGATTAACGATGCATCGCTATTTTTTGAGCCGGATACCTCAGACGCGTTAGGTTTTGGTTTCCGTTGTGGCTTCCTCGG
TATGCTGCATATGGAGATTATCCAAGAGCGCCTAGAACGCGAGTATGACTTGGATCTGATTACTACCGCGCCTTCAGTTA
TCTACGAGATTGTAAAAAAAGATGGCAGCATTATCTATGTTGATAACCCATCAAGACTGCCTGAACCGAATAATATCGAA
GAATTCCGTGAGCCGATTGCTCGTTGTCAGATTTTAGTACCGCAGGATTATCTCGGTAACGTGATGACGCTTTGCATTGA
GCGCCGCGGAGTGCAAGTCGATATGCGCTTTATGGGTCGTCAAGTGCAGCTCATTTTTGATATTCCGATGGGTGAAGTGG
TCATGGACTTCTTTGACCGTCTAAAATCAGTGTCACGTGGTTTTGCGTCACTTGATTATAATTTTGAGCGTTATCAAGTT
GATAAATTGGTTAAAGTTGATGTGCTGATTAACGGTGACAAAGTCGATGCGCTTGCTATGATTGTCCATGAAACTCAGTC
GCGCTACCGTGGTAACGCACTGGTGACCAAAATGAAAGAGCTGATTCCGCGTCAAATGTTTGATGTCGCTATTCAAGCGG
CAATTGGCAGCCAAATTATCGGGCGTAGTACTGTGAAAGCCATGCGTAAAGATGTCTTGGCTAAGTGTTATGGCGGTGAC
GTCTCGCGTAAGAAAAAGCTACTGTCTAAGCAAAAAGCCGGTAAAAAACGCATGAAGCAAGTGGGTAACGTCGAGATTCC
ACAAGAAGCCTTCTTAGCCGTATTGCAGGTAGATTAG

Upstream 100 bases:

>100_bases
GGTGTATTGACCAATATCAGCTATGAATAAGTATTCTATTGTTGCCATCTGACAGTATTTATATTCGTTATTCAATCCTC
CATTATTTAGTAGGCGCTTT

Downstream 100 bases:

>100_bases
ATTATTAATAAATGAATCATCATTGCTGTTTTTACGGATGTCATCATTGGATAAGTAGCTCGCTAATAGGCAATTAAGTA
TGGATTTTGATTTCAATTTG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MTALANIRNFSIIAHIDHGKSTLADRFIQMCGALQDREMQAQVLDSMDIERERGITIKAQSVTLYYDHPNGERYQLNFID
TPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAVDQGLEVMAVLNKIDLPQVEPERVIQEIEDIIGIDAVD
APRVSAKSGLGVDKLLEALVEFIPAPTGDRDAPLQALIIDSWFDNYLGVVSLVRVRQGTIKKGDKLYIKSTKDAHLVGSI
GVFTPKPLDTGILEAGEVGFIIAGIKDIAGAPVGDTITHASTPDVDRIPGFKQITPQVYAGMFPVESTDFEKFREALQKL
QINDASLFFEPDTSDALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPSVIYEIVKKDGSIIYVDNPSRLPEPNNIE
EFREPIARCQILVPQDYLGNVMTLCIERRGVQVDMRFMGRQVQLIFDIPMGEVVMDFFDRLKSVSRGFASLDYNFERYQV
DKLVKVDVLINGDKVDALAMIVHETQSRYRGNALVTKMKELIPRQMFDVAIQAAIGSQIIGRSTVKAMRKDVLAKCYGGD
VSRKKKLLSKQKAGKKRMKQVGNVEIPQEAFLAVLQVD

Sequences:

>Translated_598_residues
MTALANIRNFSIIAHIDHGKSTLADRFIQMCGALQDREMQAQVLDSMDIERERGITIKAQSVTLYYDHPNGERYQLNFID
TPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAVDQGLEVMAVLNKIDLPQVEPERVIQEIEDIIGIDAVD
APRVSAKSGLGVDKLLEALVEFIPAPTGDRDAPLQALIIDSWFDNYLGVVSLVRVRQGTIKKGDKLYIKSTKDAHLVGSI
GVFTPKPLDTGILEAGEVGFIIAGIKDIAGAPVGDTITHASTPDVDRIPGFKQITPQVYAGMFPVESTDFEKFREALQKL
QINDASLFFEPDTSDALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPSVIYEIVKKDGSIIYVDNPSRLPEPNNIE
EFREPIARCQILVPQDYLGNVMTLCIERRGVQVDMRFMGRQVQLIFDIPMGEVVMDFFDRLKSVSRGFASLDYNFERYQV
DKLVKVDVLINGDKVDALAMIVHETQSRYRGNALVTKMKELIPRQMFDVAIQAAIGSQIIGRSTVKAMRKDVLAKCYGGD
VSRKKKLLSKQKAGKKRMKQVGNVEIPQEAFLAVLQVD
>Mature_597_residues
TALANIRNFSIIAHIDHGKSTLADRFIQMCGALQDREMQAQVLDSMDIERERGITIKAQSVTLYYDHPNGERYQLNFIDT
PGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAVDQGLEVMAVLNKIDLPQVEPERVIQEIEDIIGIDAVDA
PRVSAKSGLGVDKLLEALVEFIPAPTGDRDAPLQALIIDSWFDNYLGVVSLVRVRQGTIKKGDKLYIKSTKDAHLVGSIG
VFTPKPLDTGILEAGEVGFIIAGIKDIAGAPVGDTITHASTPDVDRIPGFKQITPQVYAGMFPVESTDFEKFREALQKLQ
INDASLFFEPDTSDALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPSVIYEIVKKDGSIIYVDNPSRLPEPNNIEE
FREPIARCQILVPQDYLGNVMTLCIERRGVQVDMRFMGRQVQLIFDIPMGEVVMDFFDRLKSVSRGFASLDYNFERYQVD
KLVKVDVLINGDKVDALAMIVHETQSRYRGNALVTKMKELIPRQMFDVAIQAAIGSQIIGRSTVKAMRKDVLAKCYGGDV
SRKKKLLSKQKAGKKRMKQVGNVEIPQEAFLAVLQVD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=46.9217970049917, Blast_Score=592, Evalue=1e-169,
Organism=Homo sapiens, GI94966754, Length=155, Percent_Identity=41.2903225806452, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=38.255033557047, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI18390331, Length=160, Percent_Identity=35.625, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI25306283, Length=137, Percent_Identity=43.0656934306569, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI25306287, Length=137, Percent_Identity=43.0656934306569, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI19923640, Length=137, Percent_Identity=43.0656934306569, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=44.1441441441441, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=44.1441441441441, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=44.1441441441441, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI53729339, Length=223, Percent_Identity=30.9417040358744, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI53729337, Length=223, Percent_Identity=30.9417040358744, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=38.0597014925373, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI217272894, Length=134, Percent_Identity=38.0597014925373, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI94966752, Length=115, Percent_Identity=37.3913043478261, Blast_Score=70, Evalue=8e-12,
Organism=Homo sapiens, GI34147630, Length=238, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=70.03367003367, Blast_Score=852, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=508, Percent_Identity=29.9212598425197, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789738, Length=163, Percent_Identity=34.9693251533742, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1790835, Length=164, Percent_Identity=31.0975609756098, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789559, Length=223, Percent_Identity=30.9417040358744, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1790412, Length=277, Percent_Identity=29.9638989169675, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789737, Length=277, Percent_Identity=29.9638989169675, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=41.5711947626841, Blast_Score=493, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17556745, Length=158, Percent_Identity=33.5443037974684, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=252, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=138, Percent_Identity=36.231884057971, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=138, Percent_Identity=36.231884057971, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=158, Percent_Identity=34.1772151898734, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.8345864661654, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=225, Percent_Identity=30.6666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=45.3488372093023, Blast_Score=539, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=38.9261744966443, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=38.9261744966443, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=33.879781420765, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324166, Length=173, Percent_Identity=35.2601156069364, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322359, Length=117, Percent_Identity=37.6068376068376, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=283, Percent_Identity=30.0353356890459, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6325337, Length=272, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319594, Length=272, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=43.906510851419, Blast_Score=527, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24585709, Length=169, Percent_Identity=34.3195266272189, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=169, Percent_Identity=34.3195266272189, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585713, Length=169, Percent_Identity=34.3195266272189, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574573, Length=136, Percent_Identity=42.6470588235294, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24582462, Length=159, Percent_Identity=36.4779874213836, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=37.7483443708609, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=36.0902255639098, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921738, Length=252, Percent_Identity=29.7619047619048, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363316, Length=238, Percent_Identity=27.7310924369748, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17864358, Length=238, Percent_Identity=27.7310924369748, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28572034, Length=230, Percent_Identity=31.304347826087, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66444; Mature: 66313

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALANIRNFSIIAHIDHGKSTLADRFIQMCGALQDREMQAQVLDSMDIERERGITIKAQ
CCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCEEEEE
SVTLYYDHPNGERYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCY
EEEEEEECCCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
TAVDQGLEVMAVLNKIDLPQVEPERVIQEIEDIIGIDAVDAPRVSAKSGLGVDKLLEALV
HHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
EFIPAPTGDRDAPLQALIIDSWFDNYLGVVSLVRVRQGTIKKGDKLYIKSTKDAHLVGSI
HHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEE
GVFTPKPLDTGILEAGEVGFIIAGIKDIAGAPVGDTITHASTPDVDRIPGFKQITPQVYA
CCCCCCCCCCCCCCCCCCCEEEECHHHHCCCCCCCCCCCCCCCCHHHCCCHHHCCHHHHH
GMFPVESTDFEKFREALQKLQINDASLFFEPDTSDALGFGFRCGFLGMLHMEIIQERLER
CCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHH
EYDLDLITTAPSVIYEIVKKDGSIIYVDNPSRLPEPNNIEEFREPIARCQILVPQDYLGN
HCCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCEEECCHHHHHH
VMTLCIERRGVQVDMRFMGRQVQLIFDIPMGEVVMDFFDRLKSVSRGFASLDYNFERYQV
HHHHHHHHCCCEEEHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHCCCCHHHEEC
DKLVKVDVLINGDKVDALAMIVHETQSRYRGNALVTKMKELIPRQMFDVAIQAAIGSQII
CCEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRSTVKAMRKDVLAKCYGGDVSRKKKLLSKQKAGKKRMKQVGNVEIPQEAFLAVLQVD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECC
>Mature Secondary Structure 
TALANIRNFSIIAHIDHGKSTLADRFIQMCGALQDREMQAQVLDSMDIERERGITIKAQ
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCEEEEE
SVTLYYDHPNGERYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCY
EEEEEEECCCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
TAVDQGLEVMAVLNKIDLPQVEPERVIQEIEDIIGIDAVDAPRVSAKSGLGVDKLLEALV
HHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
EFIPAPTGDRDAPLQALIIDSWFDNYLGVVSLVRVRQGTIKKGDKLYIKSTKDAHLVGSI
HHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEE
GVFTPKPLDTGILEAGEVGFIIAGIKDIAGAPVGDTITHASTPDVDRIPGFKQITPQVYA
CCCCCCCCCCCCCCCCCCCEEEECHHHHCCCCCCCCCCCCCCCCHHHCCCHHHCCHHHHH
GMFPVESTDFEKFREALQKLQINDASLFFEPDTSDALGFGFRCGFLGMLHMEIIQERLER
CCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHH
EYDLDLITTAPSVIYEIVKKDGSIIYVDNPSRLPEPNNIEEFREPIARCQILVPQDYLGN
HCCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCEEECCHHHHHH
VMTLCIERRGVQVDMRFMGRQVQLIFDIPMGEVVMDFFDRLKSVSRGFASLDYNFERYQV
HHHHHHHHCCCEEEHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHCCCCHHHEEC
DKLVKVDVLINGDKVDALAMIVHETQSRYRGNALVTKMKELIPRQMFDVAIQAAIGSQII
CCEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRSTVKAMRKDVLAKCYGGDVSRKKKLLSKQKAGKKRMKQVGNVEIPQEAFLAVLQVD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA