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The map label for this gene is panB [H]

Identifier: 93004956

GI number: 93004956

Start: 148765

End: 149565

Strand: Direct

Name: panB [H]

Synonym: Pcryo_0125

Alternate gene names: 93004956

Gene position: 148765-149565 (Clockwise)

Preceding gene: 93004955

Following gene: 93004957

Centisome position: 4.86

GC content: 47.32

Gene sequence:

>801_bases
ATGACAACGTTATCTACTTTAAATAAGTTTAAAAAAGACGGTACCAAATTTACTTGTCTGACCTGTTATGACGCCATGTT
TGCGCGCATGATGGAAAAAGCGCAGATTGATACGATTTTAATCGGTGATAGCTTGGGTATGGTGGTGCAGGGTCATGATT
CAACTTTGCCTGTTACGGTCGATGATATGGCTTACCACACTGCTAATATCGCCCGTAGCAATAAACAGGCGCTTATTTTA
GCGGACTTACCCTTTATGAGTTATGTGACGCTGCCTGAGGCCGTTGTTAATAGCCGCAAGCTGATGCAGGCAGGCGCTCA
TGTGATTAAAATTGAGGGTGGTTGTGAGCTTTGCGAGTTGATTACAACATTGGCGCAAGCAGGTACGCCAACGTGCGTGC
ATTTAGGCTTAACACCGCAGTCGGTAAACGTTTTTGGCGGTTATAAAGTCCAAGGTCGCGGCAATGAAGCAGGTCAAAAA
CTCCTTGATGATGCAAAAGCTGTGGTTGACGCTGGTGCAGCGCTTTTGGTACTAGAATGCGTCCCTGCTGAGCTTGCTAA
AGCAGTAACGGAAGCCGTTGCTGTGCCAGTGATAGGCATCGGGGCAGGCGCCGATACCGATGGTCAAGTATTGGTCATGC
ACGATATGCTCGGTATGGTTCATGGTCGCGCGCCGCGTTTTATCCATGATTTTTTGACTGATGAGCGTAACACTGAGCAC
AGTATCGAAGGCGCATTTGCCTTGTATCAGCAGTCAGTAAAGGAAGGTAGTTTTCCAACAGAGCAGCACCAGTTTAGCTA
A

Upstream 100 bases:

>100_bases
CTGTTAGAACATGATGCGTCAAGTCATAAATAAAGAGACATTCATTTAAAGTGAATGACAGTTGATTGATATTCAGAAGT
ATTGAGGATGATATAACGAT

Downstream 100 bases:

>100_bases
GCAAATATAGAATTTCAGTAATTAGGTCCACCAGTAAAAGAGCAGTTAACCATGCCGACGATTCATTACCATATCTCAGA
CTTACGCGTTGCTCTACGGC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTTLSTLNKFKKDGTKFTCLTCYDAMFARMMEKAQIDTILIGDSLGMVVQGHDSTLPVTVDDMAYHTANIARSNKQALIL
ADLPFMSYVTLPEAVVNSRKLMQAGAHVIKIEGGCELCELITTLAQAGTPTCVHLGLTPQSVNVFGGYKVQGRGNEAGQK
LLDDAKAVVDAGAALLVLECVPAELAKAVTEAVAVPVIGIGAGADTDGQVLVMHDMLGMVHGRAPRFIHDFLTDERNTEH
SIEGAFALYQQSVKEGSFPTEQHQFS

Sequences:

>Translated_266_residues
MTTLSTLNKFKKDGTKFTCLTCYDAMFARMMEKAQIDTILIGDSLGMVVQGHDSTLPVTVDDMAYHTANIARSNKQALIL
ADLPFMSYVTLPEAVVNSRKLMQAGAHVIKIEGGCELCELITTLAQAGTPTCVHLGLTPQSVNVFGGYKVQGRGNEAGQK
LLDDAKAVVDAGAALLVLECVPAELAKAVTEAVAVPVIGIGAGADTDGQVLVMHDMLGMVHGRAPRFIHDFLTDERNTEH
SIEGAFALYQQSVKEGSFPTEQHQFS
>Mature_265_residues
TTLSTLNKFKKDGTKFTCLTCYDAMFARMMEKAQIDTILIGDSLGMVVQGHDSTLPVTVDDMAYHTANIARSNKQALILA
DLPFMSYVTLPEAVVNSRKLMQAGAHVIKIEGGCELCELITTLAQAGTPTCVHLGLTPQSVNVFGGYKVQGRGNEAGQKL
LDDAKAVVDAGAALLVLECVPAELAKAVTEAVAVPVIGIGAGADTDGQVLVMHDMLGMVHGRAPRFIHDFLTDERNTEHS
IEGAFALYQQSVKEGSFPTEQHQFS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=54.1666666666667, Blast_Score=292, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6319653, Length=262, Percent_Identity=33.969465648855, Blast_Score=152, Evalue=6e-38,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 28483; Mature: 28352

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLSTLNKFKKDGTKFTCLTCYDAMFARMMEKAQIDTILIGDSLGMVVQGHDSTLPVTV
CCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCEEE
DDMAYHTANIARSNKQALILADLPFMSYVTLPEAVVNSRKLMQAGAHVIKIEGGCELCEL
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
ITTLAQAGTPTCVHLGLTPQSVNVFGGYKVQGRGNEAGQKLLDDAKAVVDAGAALLVLEC
HHHHHHCCCCEEEEECCCCCCEEEEECEEEECCCCHHHHHHHHHHHHHHHCCCCEEHHHH
VPAELAKAVTEAVAVPVIGIGAGADTDGQVLVMHDMLGMVHGRAPRFIHDFLTDERNTEH
CCHHHHHHHHHHHHHCEEEECCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHCCCCCCCH
SIEGAFALYQQSVKEGSFPTEQHQFS
HHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TTLSTLNKFKKDGTKFTCLTCYDAMFARMMEKAQIDTILIGDSLGMVVQGHDSTLPVTV
CCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCEEE
DDMAYHTANIARSNKQALILADLPFMSYVTLPEAVVNSRKLMQAGAHVIKIEGGCELCEL
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
ITTLAQAGTPTCVHLGLTPQSVNVFGGYKVQGRGNEAGQKLLDDAKAVVDAGAALLVLEC
HHHHHHCCCCEEEEECCCCCCEEEEECEEEECCCCHHHHHHHHHHHHHHHCCCCEEHHHH
VPAELAKAVTEAVAVPVIGIGAGADTDGQVLVMHDMLGMVHGRAPRFIHDFLTDERNTEH
CCHHHHHHHHHHHHHCEEEECCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHCCCCCCCH
SIEGAFALYQQSVKEGSFPTEQHQFS
HHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA