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The map label for this gene is amsI [H]

Identifier: 92113826

GI number: 92113826

Start: 1935970

End: 1936410

Strand: Direct

Name: amsI [H]

Synonym: Csal_1703

Alternate gene names: 92113826

Gene position: 1935970-1936410 (Clockwise)

Preceding gene: 92113825

Following gene: 92113827

Centisome position: 52.37

GC content: 67.35

Gene sequence:

>441_bases
ATGTTCGAGCGTATTCTAGTGGTGTGCACCGGGAACATTTGCCGAAGCCCGGTGGCGGCGGCGTTGTTGCAGGCGCGCAT
GCCCGGCAAGTCGATTGCCACGGCCGGGCTGGGCGCTCGCGAGGGCGAGGGTGTCTCGCCGCGCGCGCGTGAACTGGCGG
AGGCGGAAGGGCGCGATGTGGCGAATATCCTGTCGCGTCATGTGGCACGCCAGCTCGACAAGGTCATGCTCCGCGAGGCG
GATATGGTGCTGGTCATGACCGAAGGCCAGCGGCGCGCGGTGGGCGAGATGCTGCCCGCCGCCCTGGGCAAGACCATGCT
GTTCGGCCGCTGGCTCGACAACCCCGAGATCGGCGACCCCTACGGCAAGAGCGTCGAGGCGTATACCCACGTGCATGCGC
AGTTGGTGAAAGCGGCGCGGGCGTGGGAAGGCAAGTTGTGA

Upstream 100 bases:

>100_bases
GCGCGGCCATGCCTGATGGGCGAGACTTTTCATCTTTCCGGCGGGGGCGTGGAGCCGCATCATGGGGCATTGAATCCTGT
CGCGGGAGCGTGTGTCTGTC

Downstream 100 bases:

>100_bases
TCTTGTAGTGAACAGAGCATGACACGTCAAAGCTGGCGGCCCCTTTCTGGGGCCGTTTGCGTTTCTGGGGCGGGCGTCGG
TGCCAGCGTGGCGGTGACGG

Product: protein tyrosine phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MFERILVVCTGNICRSPVAAALLQARMPGKSIATAGLGAREGEGVSPRARELAEAEGRDVANILSRHVARQLDKVMLREA
DMVLVMTEGQRRAVGEMLPAALGKTMLFGRWLDNPEIGDPYGKSVEAYTHVHAQLVKAARAWEGKL

Sequences:

>Translated_146_residues
MFERILVVCTGNICRSPVAAALLQARMPGKSIATAGLGAREGEGVSPRARELAEAEGRDVANILSRHVARQLDKVMLREA
DMVLVMTEGQRRAVGEMLPAALGKTMLFGRWLDNPEIGDPYGKSVEAYTHVHAQLVKAARAWEGKL
>Mature_146_residues
MFERILVVCTGNICRSPVAAALLQARMPGKSIATAGLGAREGEGVSPRARELAEAEGRDVANILSRHVARQLDKVMLREA
DMVLVMTEGQRRAVGEMLPAALGKTMLFGRWLDNPEIGDPYGKSVEAYTHVHAQLVKAARAWEGKL

Specific function: May function as a phosphatase required for amylovoran (an exopolysaccharide that functions as a virulence factor) production [H]

COG id: COG0394

COG function: function code T; Protein-tyrosine-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the low molecular weight phosphotyrosine protein phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788375, Length=142, Percent_Identity=42.9577464788732, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI87081812, Length=145, Percent_Identity=40, Blast_Score=104, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000106
- InterPro:   IPR017867 [H]

Pfam domain/function: PF01451 LMWPc [H]

EC number: =3.1.3.48 [H]

Molecular weight: Translated: 15850; Mature: 15850

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERILVVCTGNICRSPVAAALLQARMPGKSIATAGLGAREGEGVSPRARELAEAEGRDV
CCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCH
ANILSRHVARQLDKVMLREADMVLVMTEGQRRAVGEMLPAALGKTMLFGRWLDNPEIGDP
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YGKSVEAYTHVHAQLVKAARAWEGKL
CCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFERILVVCTGNICRSPVAAALLQARMPGKSIATAGLGAREGEGVSPRARELAEAEGRDV
CCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCH
ANILSRHVARQLDKVMLREADMVLVMTEGQRRAVGEMLPAALGKTMLFGRWLDNPEIGDP
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YGKSVEAYTHVHAQLVKAARAWEGKL
CCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]