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The map label for this gene is holB [H]

Identifier: 92113731

GI number: 92113731

Start: 1827196

End: 1828167

Strand: Direct

Name: holB [H]

Synonym: Csal_1607

Alternate gene names: 92113731

Gene position: 1827196-1828167 (Clockwise)

Preceding gene: 92113730

Following gene: 92113732

Centisome position: 49.43

GC content: 68.83

Gene sequence:

>972_bases
ATGACGCCGTTGCCCTGGCAGCGGGATCTGTGGACGCGGCTGGTGCGTCAGCAGGACGCCCAGCGTCTGCCTCACGCGCT
GCTGATCTCGGGCCCCCACGGGGTCGGCAAGCACGATCTCGCCGACGCCCTGATCGCGCGCACCCTGTGCCATGCGCCGG
GGGAGACGGCCTGCGGCGAGTGTCATGCCTGTCACATGCTGGCCTCGGGCTATCACCCCGATCTGCTGGACATCTCGCCG
GCGGAGAAGCGCCGTCAGATACGCATCGATCCGATCCGTCAGGTCAATGCCTTCGTCTCGCAGACCGCGCAGCAGGGCGG
CCACCGGGTGATTCGCCTGCATCCTGCCGAGGCCATGAACGTGGCCGCCTCCAACGCTCTGCTCAAGAGCCTGGAGGAAC
CCGGGGAAAGGACCCTGTTCGTGCTGCTGGCCGACATCCCCTCGCGCATGCTGCCGACGATTCGCTCCCGCTGCCAGCAC
TGGAGCCTGGGCGTGCCCGATGCGGAAAGCGCCTTGCCCTGGTTGAGCGAACGCCTCGAGGATGCGGAGCAGGCGCGCTT
CTGGCTGCGCGTGGCCGGCGGGCTGCCGCAGTTCGCGCTGGAGCTGGTCGAGGGGCGCGCGCTGCGCCAGGAATTGCGGG
AACTGTTCGATGCTCTGGTGCGCGGTGCCGAGCCGGTCGCCGAGGCGGCGCGCCTCGATCCCCAGTCGTTGGACCGCGTG
CTGTGGTACGGTATCGTCTGGCTCGAGGATCTGATCCGCCTGGGCGTGGCCGGCGATGCCGAGCACGTGCGCAACCCGGA
TCTGCTGCCGTTGTACCGGCAGGCAGTCAAGAACGCGCGGGTGCGCGACTGGTTTCGCCTGCTCGATTATGCCCGCGAAC
AGCGGCGCCTGATCGCGGCGGGCGGCAATCCCAACGCGCAACTGGTGCTGGAGGCCTGGCTGGTGCGCTGGGCATCGTTG
CTGCGATCTTGA

Upstream 100 bases:

>100_bases
AGGCGCCGACGCGTTTCGCCGTGATCGATGCCGATCGCAGCCTGGAGGCGGTGCAAGCGTCGATCGCCGCACACCTGACC
GAGAGGTTGGCATCATGGTC

Downstream 100 bases:

>100_bases
CGGTGCCGTGGCGTCGCCGACGGTGCGGCGATCGTGTCCGGTCGCTTCCGCATCGGTCCGATGCCCTTGTTACGGAGTGT
TCTGCGTGGCGCAAAAAGCC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTPLPWQRDLWTRLVRQQDAQRLPHALLISGPHGVGKHDLADALIARTLCHAPGETACGECHACHMLASGYHPDLLDISP
AEKRRQIRIDPIRQVNAFVSQTAQQGGHRVIRLHPAEAMNVAASNALLKSLEEPGERTLFVLLADIPSRMLPTIRSRCQH
WSLGVPDAESALPWLSERLEDAEQARFWLRVAGGLPQFALELVEGRALRQELRELFDALVRGAEPVAEAARLDPQSLDRV
LWYGIVWLEDLIRLGVAGDAEHVRNPDLLPLYRQAVKNARVRDWFRLLDYAREQRRLIAAGGNPNAQLVLEAWLVRWASL
LRS

Sequences:

>Translated_323_residues
MTPLPWQRDLWTRLVRQQDAQRLPHALLISGPHGVGKHDLADALIARTLCHAPGETACGECHACHMLASGYHPDLLDISP
AEKRRQIRIDPIRQVNAFVSQTAQQGGHRVIRLHPAEAMNVAASNALLKSLEEPGERTLFVLLADIPSRMLPTIRSRCQH
WSLGVPDAESALPWLSERLEDAEQARFWLRVAGGLPQFALELVEGRALRQELRELFDALVRGAEPVAEAARLDPQSLDRV
LWYGIVWLEDLIRLGVAGDAEHVRNPDLLPLYRQAVKNARVRDWFRLLDYAREQRRLIAAGGNPNAQLVLEAWLVRWASL
LRS
>Mature_322_residues
TPLPWQRDLWTRLVRQQDAQRLPHALLISGPHGVGKHDLADALIARTLCHAPGETACGECHACHMLASGYHPDLLDISPA
EKRRQIRIDPIRQVNAFVSQTAQQGGHRVIRLHPAEAMNVAASNALLKSLEEPGERTLFVLLADIPSRMLPTIRSRCQHW
SLGVPDAESALPWLSERLEDAEQARFWLRVAGGLPQFALELVEGRALRQELRELFDALVRGAEPVAEAARLDPQSLDRVL
WYGIVWLEDLIRLGVAGDAEHVRNPDLLPLYRQAVKNARVRDWFRLLDYAREQRRLIAAGGNPNAQLVLEAWLVRWASLL
RS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=317, Percent_Identity=31.2302839116719, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786676, Length=165, Percent_Identity=32.7272727272727, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36386; Mature: 36255

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLPWQRDLWTRLVRQQDAQRLPHALLISGPHGVGKHDLADALIARTLCHAPGETACGE
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCH
CHACHMLASGYHPDLLDISPAEKRRQIRIDPIRQVNAFVSQTAQQGGHRVIRLHPAEAMN
HHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHH
VAASNALLKSLEEPGERTLFVLLADIPSRMLPTIRSRCQHWSLGVPDAESALPWLSERLE
HHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DAEQARFWLRVAGGLPQFALELVEGRALRQELRELFDALVRGAEPVAEAARLDPQSLDRV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHH
LWYGIVWLEDLIRLGVAGDAEHVRNPDLLPLYRQAVKNARVRDWFRLLDYAREQRRLIAA
HHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCEEEEC
GGNPNAQLVLEAWLVRWASLLRS
CCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPLPWQRDLWTRLVRQQDAQRLPHALLISGPHGVGKHDLADALIARTLCHAPGETACGE
CCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCH
CHACHMLASGYHPDLLDISPAEKRRQIRIDPIRQVNAFVSQTAQQGGHRVIRLHPAEAMN
HHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHH
VAASNALLKSLEEPGERTLFVLLADIPSRMLPTIRSRCQHWSLGVPDAESALPWLSERLE
HHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DAEQARFWLRVAGGLPQFALELVEGRALRQELRELFDALVRGAEPVAEAARLDPQSLDRV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHH
LWYGIVWLEDLIRLGVAGDAEHVRNPDLLPLYRQAVKNARVRDWFRLLDYAREQRRLIAA
HHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCEEEEC
GGNPNAQLVLEAWLVRWASLLRS
CCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]