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The map label for this gene is speE [H]

Identifier: 92112254

GI number: 92112254

Start: 141196

End: 142074

Strand: Direct

Name: speE [H]

Synonym: Csal_0119

Alternate gene names: 92112254

Gene position: 141196-142074 (Clockwise)

Preceding gene: 92112253

Following gene: 92112255

Centisome position: 3.82

GC content: 63.25

Gene sequence:

>879_bases
ATGACGAGGATTCCCATGACCGAGCGTCTCGATCAGGGCTGGTTCACCGAAGTGTTCGACAGCCATGGCAGTGCTTTTTC
CCTCATGATCAAGGACAAGCTGCACGAAGTGCAGAGTCCCTATCAGCACCTGGAGGTCTACGCGACCGAGACCTACGGCA
ATCTGATGGTGCTCGACGGCTGCGTGATGCTCAGCACGCGCGACAATTTTCTCTATCACGAGATGATGGCGCATCCGGCG
CTGTTCGCGCATGCCGCGCCCAAGCGTGTGGTGATCATCGGCGGCGGCGATTGCGGAACGCTGCGCGAGGTGCTCAAGCA
CCCCGGCGTCGAGCAGGTCGTGCAGATCGATATCGATGAAGAAGTCACGCGCGCCTCGCAGCGTTATTTCCCGGAGTTGA
CGGCTTCCAACGACGACCCGCGCGCCACGCTCAAGTTCGAGGACGGTGTCAAATGGGTCGAGAGCGCGCCGGAAGGCAGC
GTCGACGTCATGATCATCGACTCGACCGATCCGGTGGGGCCGGCGGAAGGACTGTTCGAGGTCGACTTCCTGACGCGTTG
CCACCGGCTGCTCGCCGACGGCGGCGTGCTGGTGCAGCAAAGCGAGTCGCCGCTGTATCACACCGGCTCGATCATTCGCG
AATTGCGCGCCGACATGCAACGCGCGGGCTTCGATAGCGTCGCCACGCTGCCGTTCCCGCAGCCGGTCTACCCCTCCGGA
TGGTGGAGCGCGACTCTGGCCGGCAAGGGCAGTGACGTGACGCGCTTTCGCGAACGGGATGCCGCCGCGGTGGAATTCCC
GCTCGAGTACTACAGCGTCGAGACCCATCGTGGTGCATTGGCGCTGCCGCCGTTCATGCGTCGTGCACTATCGGCGTGA

Upstream 100 bases:

>100_bases
TTGTTGCGCACGGATAGGCTATGTTGCGCGGTTTTCCGGTGCGTTCGCGGCAGGCCGTGAACGCCGCGACTCCGGCCCCT
CGAGGGCTTTCACTAGGGCG

Downstream 100 bases:

>100_bases
TGCGAAACGTGTGAAAGGCGTTAATGACACGTGACTTAGGTATATTGGCTGGGATGGCAAGCTCTTTGCTAATGTGATGC
GTGGCTCTCGTATAAGCCAC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTRIPMTERLDQGWFTEVFDSHGSAFSLMIKDKLHEVQSPYQHLEVYATETYGNLMVLDGCVMLSTRDNFLYHEMMAHPA
LFAHAAPKRVVIIGGGDCGTLREVLKHPGVEQVVQIDIDEEVTRASQRYFPELTASNDDPRATLKFEDGVKWVESAPEGS
VDVMIIDSTDPVGPAEGLFEVDFLTRCHRLLADGGVLVQQSESPLYHTGSIIRELRADMQRAGFDSVATLPFPQPVYPSG
WWSATLAGKGSDVTRFRERDAAAVEFPLEYYSVETHRGALALPPFMRRALSA

Sequences:

>Translated_292_residues
MTRIPMTERLDQGWFTEVFDSHGSAFSLMIKDKLHEVQSPYQHLEVYATETYGNLMVLDGCVMLSTRDNFLYHEMMAHPA
LFAHAAPKRVVIIGGGDCGTLREVLKHPGVEQVVQIDIDEEVTRASQRYFPELTASNDDPRATLKFEDGVKWVESAPEGS
VDVMIIDSTDPVGPAEGLFEVDFLTRCHRLLADGGVLVQQSESPLYHTGSIIRELRADMQRAGFDSVATLPFPQPVYPSG
WWSATLAGKGSDVTRFRERDAAAVEFPLEYYSVETHRGALALPPFMRRALSA
>Mature_291_residues
TRIPMTERLDQGWFTEVFDSHGSAFSLMIKDKLHEVQSPYQHLEVYATETYGNLMVLDGCVMLSTRDNFLYHEMMAHPAL
FAHAAPKRVVIIGGGDCGTLREVLKHPGVEQVVQIDIDEEVTRASQRYFPELTASNDDPRATLKFEDGVKWVESAPEGSV
DVMIIDSTDPVGPAEGLFEVDFLTRCHRLLADGGVLVQQSESPLYHTGSIIRELRADMQRAGFDSVATLPFPQPVYPSGW
WSATLAGKGSDVTRFRERDAAAVEFPLEYYSVETHRGALALPPFMRRALSA

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=288, Percent_Identity=38.5416666666667, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1786312, Length=283, Percent_Identity=33.5689045936396, Blast_Score=158, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI25154106, Length=272, Percent_Identity=38.9705882352941, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI212645616, Length=323, Percent_Identity=33.4365325077399, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325326, Length=286, Percent_Identity=36.7132867132867, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6323175, Length=291, Percent_Identity=32.6460481099656, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24645443, Length=285, Percent_Identity=33.3333333333333, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI21355941, Length=216, Percent_Identity=31.9444444444444, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32564; Mature: 32433

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIPMTERLDQGWFTEVFDSHGSAFSLMIKDKLHEVQSPYQHLEVYATETYGNLMVLDG
CCCCCCHHHHCCCHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHEEEEEECCCCCEEEEEC
CVMLSTRDNFLYHEMMAHPALFAHAAPKRVVIIGGGDCGTLREVLKHPGVEQVVQIDIDE
EEEEECCCCHHHHHHHHCHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCH
EVTRASQRYFPELTASNDDPRATLKFEDGVKWVESAPEGSVDVMIIDSTDPVGPAEGLFE
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCEEEEEEECCCCCCCCCCEEE
VDFLTRCHRLLADGGVLVQQSESPLYHTGSIIRELRADMQRAGFDSVATLPFPQPVYPSG
HHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
WWSATLAGKGSDVTRFRERDAAAVEFPLEYYSVETHRGALALPPFMRRALSA
CCEEEECCCCCCHHHHHHCCCCEEEECHHHEEEECCCCCEECCHHHHHHHCC
>Mature Secondary Structure 
TRIPMTERLDQGWFTEVFDSHGSAFSLMIKDKLHEVQSPYQHLEVYATETYGNLMVLDG
CCCCCHHHHCCCHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHEEEEEECCCCCEEEEEC
CVMLSTRDNFLYHEMMAHPALFAHAAPKRVVIIGGGDCGTLREVLKHPGVEQVVQIDIDE
EEEEECCCCHHHHHHHHCHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCH
EVTRASQRYFPELTASNDDPRATLKFEDGVKWVESAPEGSVDVMIIDSTDPVGPAEGLFE
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCEEEEEEECCCCCCCCCCEEE
VDFLTRCHRLLADGGVLVQQSESPLYHTGSIIRELRADMQRAGFDSVATLPFPQPVYPSG
HHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
WWSATLAGKGSDVTRFRERDAAAVEFPLEYYSVETHRGALALPPFMRRALSA
CCEEEECCCCCCHHHHHHCCCCEEEECHHHEEEECCCCCEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA