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The map label for this gene is hipO [H]

Identifier: 91790355

GI number: 91790355

Start: 4782416

End: 4783588

Strand: Reverse

Name: hipO [H]

Synonym: Bpro_4523

Alternate gene names: 91790355

Gene position: 4783588-4782416 (Counterclockwise)

Preceding gene: 91790356

Following gene: 91790350

Centisome position: 91.99

GC content: 69.82

Gene sequence:

>1173_bases
ATGAGCCCCTTGCTTGAAGAACTGCACGCGCAGGCCGATGAGTTCATCGGCCTGCGGCGCGACATCCACCGCCACCCCGA
ACTGGCCTTCGACGAGCACCGCACCTCGGCCCTGGTGGCCGAGAAGCTGCAAGGCTGGGGCTACGCGGTCGAGCGCGGCC
TGGGCGGCACCGGCTTGGTCGGCCGGCTGGTGCGTGGCGACGGCCAGCGCCGGCTCGGGCTGCGCGCCGACATGGACGCC
CTGCCCATCCACGAGGCCACCGGCCTGCCGCACGCCAGTTGCCATGCGGGCGTGATGCACGCCTGCGGCCACGACGGCCA
CACCGCCATGCTGCTGGCCGCCGCGCGCCACCTCGCCGAGCATGGGCGCTTTTCCGGCACCTTGAACCTGATCTTCCAGC
CGGCCGAAGAAGGCGGCGGCGGCGCGCTGCACATGATGGACGACGGCCTGTTCGAGAAGTACCCCTGCGATGCGGTCTTC
GCCATGCACAACATGCCCGGCATACCGCAGGGCCGGCTGGTGCTGCGCGAAGGCCCGGCCATGGCCTCGTCAGACTACGC
CACCGTCACGCTGACCGGCGTGGGTGGTCATGGCGCCATGCCGCACCGCGCGGCCGACCCCGTTGTGGCGGCCGCCAGCA
TCGTGATGGCGCTGCAGACCATCGTCTCGCGCAACATCGACCCGCTGCAGATGGCGGTGGTCACGGTGGGCGCGCTGCAC
GCCGGCAAGGCCAACAACGTGATCCCGCAAAGCGCGACGCTGGAGCTCAGCGTGCGCGCGCTGGACCGAGAGGTGCGCAG
CAAGCTGGAGCAGCGCATCAGGGCGTTGATCGCCGCGCAGGCCGAAAGCTTTGAGGTGCAGGCGCAGATAGCCTGGCGGC
CCGGTTACGCGGTGCTGGTCAACACACCCGGCGAAACCGCCTTTGCCCGCGAGGTGGCGCTGGAGCTGGTCGGCGCCGGG
CGCGTGACCCTGCAGGGCCCGGCCGTGTCCGGCAGTGAAGACTTCGCCTTCATGCTGGAGCGCGTGCCCGGCAGTTATCT
GTTTATCGGCAACGGCGATGGCGACAGCGCCGGCGCCTGTATGGTGCACAACCCAGGCTACGACTTCAACGATGCCAACC
TGCCCGTGGGCGCGGCCTACTGGGTGCTGCTGGCGCAACGCTTTCTGGTGTGA

Upstream 100 bases:

>100_bases
CCTGTCGCTGGCCGAATCGGCGAAGTTCTTCGAGGCCGAGACCGCGCGCTACCGCAGTATCGCCAAATCGATCAATCTGC
AGCCGCAGTGAGGCACGCCC

Downstream 100 bases:

>100_bases
ACAAGCCGCAGTGCCCTGAAAGAACGGCGCCCGAGGGCGCCGTTCTTTTTTCCATTAACTCTTCAAGTTAATTTGGTCAG
TAGTCTCTGCCAGTATTACG

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSPLLEELHAQADEFIGLRRDIHRHPELAFDEHRTSALVAEKLQGWGYAVERGLGGTGLVGRLVRGDGQRRLGLRADMDA
LPIHEATGLPHASCHAGVMHACGHDGHTAMLLAAARHLAEHGRFSGTLNLIFQPAEEGGGGALHMMDDGLFEKYPCDAVF
AMHNMPGIPQGRLVLREGPAMASSDYATVTLTGVGGHGAMPHRAADPVVAAASIVMALQTIVSRNIDPLQMAVVTVGALH
AGKANNVIPQSATLELSVRALDREVRSKLEQRIRALIAAQAESFEVQAQIAWRPGYAVLVNTPGETAFAREVALELVGAG
RVTLQGPAVSGSEDFAFMLERVPGSYLFIGNGDGDSAGACMVHNPGYDFNDANLPVGAAYWVLLAQRFLV

Sequences:

>Translated_390_residues
MSPLLEELHAQADEFIGLRRDIHRHPELAFDEHRTSALVAEKLQGWGYAVERGLGGTGLVGRLVRGDGQRRLGLRADMDA
LPIHEATGLPHASCHAGVMHACGHDGHTAMLLAAARHLAEHGRFSGTLNLIFQPAEEGGGGALHMMDDGLFEKYPCDAVF
AMHNMPGIPQGRLVLREGPAMASSDYATVTLTGVGGHGAMPHRAADPVVAAASIVMALQTIVSRNIDPLQMAVVTVGALH
AGKANNVIPQSATLELSVRALDREVRSKLEQRIRALIAAQAESFEVQAQIAWRPGYAVLVNTPGETAFAREVALELVGAG
RVTLQGPAVSGSEDFAFMLERVPGSYLFIGNGDGDSAGACMVHNPGYDFNDANLPVGAAYWVLLAQRFLV
>Mature_389_residues
SPLLEELHAQADEFIGLRRDIHRHPELAFDEHRTSALVAEKLQGWGYAVERGLGGTGLVGRLVRGDGQRRLGLRADMDAL
PIHEATGLPHASCHAGVMHACGHDGHTAMLLAAARHLAEHGRFSGTLNLIFQPAEEGGGGALHMMDDGLFEKYPCDAVFA
MHNMPGIPQGRLVLREGPAMASSDYATVTLTGVGGHGAMPHRAADPVVAAASIVMALQTIVSRNIDPLQMAVVTVGALHA
GKANNVIPQSATLELSVRALDREVRSKLEQRIRALIAAQAESFEVQAQIAWRPGYAVLVNTPGETAFAREVALELVGAGR
VTLQGPAVSGSEDFAFMLERVPGSYLFIGNGDGDSAGACMVHNPGYDFNDANLPVGAAYWVLLAQRFLV

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=427, Percent_Identity=29.03981264637, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41457; Mature: 41326

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLLEELHAQADEFIGLRRDIHRHPELAFDEHRTSALVAEKLQGWGYAVERGLGGTGLV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHH
GRLVRGDGQRRLGLRADMDALPIHEATGLPHASCHAGVMHACGHDGHTAMLLAAARHLAE
HHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HGRFSGTLNLIFQPAEEGGGGALHMMDDGLFEKYPCDAVFAMHNMPGIPQGRLVLREGPA
CCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCC
MASSDYATVTLTGVGGHGAMPHRAADPVVAAASIVMALQTIVSRNIDPLQMAVVTVGALH
CCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
AGKANNVIPQSATLELSVRALDREVRSKLEQRIRALIAAQAESFEVQAQIAWRPGYAVLV
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEE
NTPGETAFAREVALELVGAGRVTLQGPAVSGSEDFAFMLERVPGSYLFIGNGDGDSAGAC
ECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEE
MVHNPGYDFNDANLPVGAAYWVLLAQRFLV
EEECCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPLLEELHAQADEFIGLRRDIHRHPELAFDEHRTSALVAEKLQGWGYAVERGLGGTGLV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHH
GRLVRGDGQRRLGLRADMDALPIHEATGLPHASCHAGVMHACGHDGHTAMLLAAARHLAE
HHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HGRFSGTLNLIFQPAEEGGGGALHMMDDGLFEKYPCDAVFAMHNMPGIPQGRLVLREGPA
CCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCC
MASSDYATVTLTGVGGHGAMPHRAADPVVAAASIVMALQTIVSRNIDPLQMAVVTVGALH
CCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
AGKANNVIPQSATLELSVRALDREVRSKLEQRIRALIAAQAESFEVQAQIAWRPGYAVLV
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEE
NTPGETAFAREVALELVGAGRVTLQGPAVSGSEDFAFMLERVPGSYLFIGNGDGDSAGAC
ECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEE
MVHNPGYDFNDANLPVGAAYWVLLAQRFLV
EEECCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]