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The map label for this gene is cysS

Identifier: 91788726

GI number: 91788726

Start: 3020266

End: 3021654

Strand: Reverse

Name: cysS

Synonym: Bpro_2865

Alternate gene names: 91788726

Gene position: 3021654-3020266 (Counterclockwise)

Preceding gene: 91788732

Following gene: 91788725

Centisome position: 58.11

GC content: 61.2

Gene sequence:

>1389_bases
ATGAGCCTTCGCATTTACAACACGCTGTCGCGTGACGTAGAAGTTTTTTCTCCACTGCTTCCCGGCCACGTGCGCATGTA
TGTGTGCGGCATGACGGTGTATGACCTCTGCCATCTGGGCCATGCACGCGCCATGGTGGCTTTTGATGTGGTCCAGCGCT
GGCTCAAGGTGAGCGGGTTGAAGGTGACTTACGTGCGCAACGTGACCGACATTGACGACAAGATCATCAAGCGGGCCGTG
GAAAACGGTGAAACCATCCGATCGCTGACCGACCGCATGGTGGTCGCCCTGCACCAGGATGCAGATGCGCTGGGCATTGA
GCGCCCCACCCACGAGCCGCGCGCCACCGACTTTGTGCCGCAGATGCTCAGCCTGATCGGCACCCTGGAGAAAAAGGGAC
TGGCCTATCAAACCCAAAGCGGCTCGGGCCAGGGCGACGTGAACTACGCCGTGCGCAAGTTTGACGGCTACGGCAAGCTG
TCCGGCAAGTCGCTGGATGAGTTGCGGGCCGGTGAGCGGGTGGAAGTGCTTGATGGCAAGGACGATCCGCTGGACTTTGT
GCTCTGGAAAAGCGCCAAGCCGAGCGAGCCCGATGACGCCAAGTGGGACAGCCCCTACGGCCCCGGCCGGCCCGGCTGGC
ATATTGAATGTTCGGCCATGGCCTGCGAGTTGCTCGGGGAGTCGTTTGACATCCACGGCGGCGGCGCCGATCTGCAGTTC
CCGCACCACGAAAACGAAATTGCCCAGTCGGAAGGAGCCTTTGGCAAGCCCCTGGCCGGCACCTGGATGCACAACGGCTT
CGTGCGGGTGGACAACGAAAAGATGTCCAAGTCACTGGGGAATTTTTTCACCATCCGTGAAATCCTGGCGAAGTACGACG
CCGAGACCGTGCGTTTTTTCATCATTCGAGCCCACTACCGCAGTCCGCTCAATTACAGCGATGCTCACCTGGATGACGCC
CGCAATTCGCTCAAGCGCCTCTATACCGCGCTGGACCTCGTCAGCCCTGCCGTGGTGGCGATTGACTGGACCAACCCGTT
TGCGGCGCGCTTCAAGGCCGCCATGGATGAAGACTTCGGCACACCGGAAGCGGTGGCGGTGCTGTTCGATCTGGCCGGTG
AAGTCAACAAAACCCGTTCTGCCGAGCTGGCTGGTTTGCTGAAGAGCCTGGGCGGCTGCCTGGGCTTGCTGCAGAGCGCC
CCCGGCAGCTACCTCCAGGCCGGCGCCGGGCTGGACGATGCCAGCATCCAGCGGCTGATCGCTCAGCGCGCCGATGCCAA
GAAAACGAAAAACTTCGCCGAGGCAGACCGCATCCGCAATGATTTGCTGGGTCAGGGCATTGTGCTCAAGGATTCACCGG
CAGGCACCACCTGGGAAGTGCAATCGTGA

Upstream 100 bases:

>100_bases
TTTACCTTTGCCTGCGCCTGTAACCGTTCGTTGTATTTCGCTTCTTGTCCCCTTCTGACCGATCGACCGATTCTCGTCAG
GCCACGCATTCAGACACTCC

Downstream 100 bases:

>100_bases
GGTTGGGGCTTAAATATTCAGATCATTCTGGCCACCAGTTGCTGCAGCGCGGGTGCGGGCAGTTATGAAAAAGATAGTAA
AAACCGGCCCTGCCAGTGTT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSLRIYNTLSRDVEVFSPLLPGHVRMYVCGMTVYDLCHLGHARAMVAFDVVQRWLKVSGLKVTYVRNVTDIDDKIIKRAV
ENGETIRSLTDRMVVALHQDADALGIERPTHEPRATDFVPQMLSLIGTLEKKGLAYQTQSGSGQGDVNYAVRKFDGYGKL
SGKSLDELRAGERVEVLDGKDDPLDFVLWKSAKPSEPDDAKWDSPYGPGRPGWHIECSAMACELLGESFDIHGGGADLQF
PHHENEIAQSEGAFGKPLAGTWMHNGFVRVDNEKMSKSLGNFFTIREILAKYDAETVRFFIIRAHYRSPLNYSDAHLDDA
RNSLKRLYTALDLVSPAVVAIDWTNPFAARFKAAMDEDFGTPEAVAVLFDLAGEVNKTRSAELAGLLKSLGGCLGLLQSA
PGSYLQAGAGLDDASIQRLIAQRADAKKTKNFAEADRIRNDLLGQGIVLKDSPAGTTWEVQS

Sequences:

>Translated_462_residues
MSLRIYNTLSRDVEVFSPLLPGHVRMYVCGMTVYDLCHLGHARAMVAFDVVQRWLKVSGLKVTYVRNVTDIDDKIIKRAV
ENGETIRSLTDRMVVALHQDADALGIERPTHEPRATDFVPQMLSLIGTLEKKGLAYQTQSGSGQGDVNYAVRKFDGYGKL
SGKSLDELRAGERVEVLDGKDDPLDFVLWKSAKPSEPDDAKWDSPYGPGRPGWHIECSAMACELLGESFDIHGGGADLQF
PHHENEIAQSEGAFGKPLAGTWMHNGFVRVDNEKMSKSLGNFFTIREILAKYDAETVRFFIIRAHYRSPLNYSDAHLDDA
RNSLKRLYTALDLVSPAVVAIDWTNPFAARFKAAMDEDFGTPEAVAVLFDLAGEVNKTRSAELAGLLKSLGGCLGLLQSA
PGSYLQAGAGLDDASIQRLIAQRADAKKTKNFAEADRIRNDLLGQGIVLKDSPAGTTWEVQS
>Mature_461_residues
SLRIYNTLSRDVEVFSPLLPGHVRMYVCGMTVYDLCHLGHARAMVAFDVVQRWLKVSGLKVTYVRNVTDIDDKIIKRAVE
NGETIRSLTDRMVVALHQDADALGIERPTHEPRATDFVPQMLSLIGTLEKKGLAYQTQSGSGQGDVNYAVRKFDGYGKLS
GKSLDELRAGERVEVLDGKDDPLDFVLWKSAKPSEPDDAKWDSPYGPGRPGWHIECSAMACELLGESFDIHGGGADLQFP
HHENEIAQSEGAFGKPLAGTWMHNGFVRVDNEKMSKSLGNFFTIREILAKYDAETVRFFIIRAHYRSPLNYSDAHLDDAR
NSLKRLYTALDLVSPAVVAIDWTNPFAARFKAAMDEDFGTPEAVAVLFDLAGEVNKTRSAELAGLLKSLGGCLGLLQSAP
GSYLQAGAGLDDASIQRLIAQRADAKKTKNFAEADRIRNDLLGQGIVLKDSPAGTTWEVQS

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=405, Percent_Identity=39.5061728395062, Blast_Score=236, Evalue=5e-62,
Organism=Homo sapiens, GI21269877, Length=233, Percent_Identity=38.1974248927039, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI303304987, Length=233, Percent_Identity=38.1974248927039, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI62240992, Length=233, Percent_Identity=38.1974248927039, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI10835051, Length=233, Percent_Identity=38.1974248927039, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1786737, Length=465, Percent_Identity=54.6236559139785, Blast_Score=482, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17509797, Length=222, Percent_Identity=36.9369369369369, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17509795, Length=222, Percent_Identity=36.9369369369369, Blast_Score=149, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324082, Length=452, Percent_Identity=28.7610619469027, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI19922218, Length=497, Percent_Identity=32.3943661971831, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI19922364, Length=241, Percent_Identity=40.6639004149378, Blast_Score=165, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_POLSJ (Q129L2)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549678.1
- ProteinModelPortal:   Q129L2
- SMR:   Q129L2
- STRING:   Q129L2
- GeneID:   4014478
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2865
- NMPDR:   fig|296591.1.peg.5456
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q129L2
- ProtClustDB:   PRK00260
- BioCyc:   PSP296591:BPRO_2865-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 50834; Mature: 50703

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRIYNTLSRDVEVFSPLLPGHVRMYVCGMTVYDLCHLGHARAMVAFDVVQRWLKVSGL
CCEEEEHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCE
KVTYVRNVTDIDDKIIKRAVENGETIRSLTDRMVVALHQDADALGIERPTHEPRATDFVP
EEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHH
QMLSLIGTLEKKGLAYQTQSGSGQGDVNYAVRKFDGYGKLSGKSLDELRAGERVEVLDGK
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHEEEECCCCCCCCCCHHHHHCCCEEEEECCC
DDPLDFVLWKSAKPSEPDDAKWDSPYGPGRPGWHIECSAMACELLGESFDIHGGGADLQF
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEECCCCCCCCC
PHHENEIAQSEGAFGKPLAGTWMHNGFVRVDNEKMSKSLGNFFTIREILAKYDAETVRFF
CCCCCHHHHCCCCCCCCCCCHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
IIRAHYRSPLNYSDAHLDDARNSLKRLYTALDLVSPAVVAIDWTNPFAARFKAAMDEDFG
EEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCC
TPEAVAVLFDLAGEVNKTRSAELAGLLKSLGGCLGLLQSAPGSYLQAGAGLDDASIQRLI
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHH
AQRADAKKTKNFAEADRIRNDLLGQGIVLKDSPAGTTWEVQS
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECC
>Mature Secondary Structure 
SLRIYNTLSRDVEVFSPLLPGHVRMYVCGMTVYDLCHLGHARAMVAFDVVQRWLKVSGL
CEEEEHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCE
KVTYVRNVTDIDDKIIKRAVENGETIRSLTDRMVVALHQDADALGIERPTHEPRATDFVP
EEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHH
QMLSLIGTLEKKGLAYQTQSGSGQGDVNYAVRKFDGYGKLSGKSLDELRAGERVEVLDGK
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHEEEECCCCCCCCCCHHHHHCCCEEEEECCC
DDPLDFVLWKSAKPSEPDDAKWDSPYGPGRPGWHIECSAMACELLGESFDIHGGGADLQF
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEECCCCCCCCC
PHHENEIAQSEGAFGKPLAGTWMHNGFVRVDNEKMSKSLGNFFTIREILAKYDAETVRFF
CCCCCHHHHCCCCCCCCCCCHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
IIRAHYRSPLNYSDAHLDDARNSLKRLYTALDLVSPAVVAIDWTNPFAARFKAAMDEDFG
EEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCC
TPEAVAVLFDLAGEVNKTRSAELAGLLKSLGGCLGLLQSAPGSYLQAGAGLDDASIQRLI
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHH
AQRADAKKTKNFAEADRIRNDLLGQGIVLKDSPAGTTWEVQS
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA