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The map label for this gene is phoB [H]

Identifier: 91788128

GI number: 91788128

Start: 2343767

End: 2344498

Strand: Direct

Name: phoB [H]

Synonym: Bpro_2257

Alternate gene names: 91788128

Gene position: 2343767-2344498 (Clockwise)

Preceding gene: 91788127

Following gene: 91788129

Centisome position: 45.07

GC content: 61.89

Gene sequence:

>732_bases
ATGAAACACCAAGCCCGCATACTGATTGTTGAAGACGAGCCAGCGATTGCCGAACTGATTGCGGTCAACTTGAGGCATGG
TGGGTTTGAGCCCGTCGTGGTTCAGGACAGTGTGGCGGCCCAGCGCGAACTCGACGCGGTGCTGCCCGATGCCATCCTGC
TCGACTGGATGCTGCCGGGGCAGAGCGGCTTGGCGCTGGCGCGTCATTGGCGCAAGCAGGTACGCACCAAGGCCATTCCC
ATCCTGATGCTGACCGCCCGCGGCGATGAGCCTGACAAGGTTGCGGGCCTGGATGCCGGTGCCGACGACTACATCACCAA
GCCGTTTTCAACGCAGGAGCTGCTCGCGCGCATTCGTGCCGTGCTGCGCCGCCGCGCACCGGAGTCGGTGAACGACAGTG
TGACAGTGGGTGCGCTGGTACTGGATGCAGGCACCCATCGCATTTCGTTCCAGAACCAGGAACTCAAGCTGGGCCCGACG
GAGTTCAAGCTGCTGCATTACCTGATGAAACATGCGGAACGTGTGCACAGCCGCAGTACCTTGCTGGACAAGGTGTGGGG
TGACCATGTGTTCATTGAAGAACGTACGGTAGACGTTCACGTCAAGCGCCTGCGTGAGGCGCTGGGCGCCGCCGGTACCA
TGGTTGAAACCGTTCGCGGGGCGGGTTACCGTCTGACTGCTGCGGCTGCCGCTACCACTCGGGTAACTGCTGCGGCACAA
TCCGGCACATGA

Upstream 100 bases:

>100_bases
CGCCAAGAATATCGCCGAGTTCATCATCTATATCGTCAAAGGCGCGGATGTCAGGCATACCTCCATGGACCAGATCGAGT
CCGCCGTCAAGTAAGGCCGC

Downstream 100 bases:

>100_bases
TCAACCGCTGGATCGCTTTTACCGCCTGCATGTTGGCCGGTGGCGCATTGGGATGGTTGACGACTGCGAAGGGTGGACCC
GCATGGAGCCTGGGTGTGGG

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKHQARILIVEDEPAIAELIAVNLRHGGFEPVVVQDSVAAQRELDAVLPDAILLDWMLPGQSGLALARHWRKQVRTKAIP
ILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRAVLRRRAPESVNDSVTVGALVLDAGTHRISFQNQELKLGPT
EFKLLHYLMKHAERVHSRSTLLDKVWGDHVFIEERTVDVHVKRLREALGAAGTMVETVRGAGYRLTAAAAATTRVTAAAQ
SGT

Sequences:

>Translated_243_residues
MKHQARILIVEDEPAIAELIAVNLRHGGFEPVVVQDSVAAQRELDAVLPDAILLDWMLPGQSGLALARHWRKQVRTKAIP
ILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRAVLRRRAPESVNDSVTVGALVLDAGTHRISFQNQELKLGPT
EFKLLHYLMKHAERVHSRSTLLDKVWGDHVFIEERTVDVHVKRLREALGAAGTMVETVRGAGYRLTAAAAATTRVTAAAQ
SGT
>Mature_243_residues
MKHQARILIVEDEPAIAELIAVNLRHGGFEPVVVQDSVAAQRELDAVLPDAILLDWMLPGQSGLALARHWRKQVRTKAIP
ILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRAVLRRRAPESVNDSVTVGALVLDAGTHRISFQNQELKLGPT
EFKLLHYLMKHAERVHSRSTLLDKVWGDHVFIEERTVDVHVKRLREALGAAGTMVETVRGAGYRLTAAAAATTRVTAAAQ
SGT

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=224, Percent_Identity=52.2321428571429, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1788394, Length=233, Percent_Identity=38.6266094420601, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789809, Length=236, Percent_Identity=36.4406779661017, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=38.5650224215247, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=38.4615384615385, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=35.9090909090909, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=36.6071428571429, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=34.6846846846847, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082012, Length=228, Percent_Identity=35.0877192982456, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=32.7272727272727, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=35.9649122807018, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1790863, Length=233, Percent_Identity=31.7596566523605, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=30.2631578947368, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=29.004329004329, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26640; Mature: 26640

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHQARILIVEDEPAIAELIAVNLRHGGFEPVVVQDSVAAQRELDAVLPDAILLDWMLPG
CCCCEEEEEEECCCHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHCCCHHHHHEECCC
QSGLALARHWRKQVRTKAIPILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRA
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHH
VLRRRAPESVNDSVTVGALVLDAGTHRISFQNQELKLGPTEFKLLHYLMKHAERVHSRST
HHHHCCCCCCCCCEEEEEEEEECCCCEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHH
LLDKVWGDHVFIEERTVDVHVKRLREALGAAGTMVETVRGAGYRLTAAAAATTRVTAAAQ
HHHHHCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEHHHHHHHHEECCC
SGT
CCC
>Mature Secondary Structure
MKHQARILIVEDEPAIAELIAVNLRHGGFEPVVVQDSVAAQRELDAVLPDAILLDWMLPG
CCCCEEEEEEECCCHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHCCCHHHHHEECCC
QSGLALARHWRKQVRTKAIPILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRA
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHH
VLRRRAPESVNDSVTVGALVLDAGTHRISFQNQELKLGPTEFKLLHYLMKHAERVHSRST
HHHHCCCCCCCCCEEEEEEEEECCCCEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHH
LLDKVWGDHVFIEERTVDVHVKRLREALGAAGTMVETVRGAGYRLTAAAAATTRVTAAAQ
HHHHHCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEHHHHHHHHEECCC
SGT
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]