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The map label for this gene is mdeA [H]

Identifier: 91787715

GI number: 91787715

Start: 1896891

End: 1898192

Strand: Reverse

Name: mdeA [H]

Synonym: Bpro_1836

Alternate gene names: 91787715

Gene position: 1898192-1896891 (Counterclockwise)

Preceding gene: 91787717

Following gene: 91787714

Centisome position: 36.5

GC content: 64.82

Gene sequence:

>1302_bases
ATGTCCGGCTACTCCGACCCCGGTTTCGACACCCTGGCGCTGCACGCAGGTGCCGCACCCGACCCCGCCACGGGCGCCCG
CGCGGTGCCGATTTATCTCACCACCTCCTTTGTCTTTGAGTCGAGCGACCACGCCGCCAGCCTCTTTAACCTGGAGCGTG
CGGGCCACGTGTACTCACGCATCAGCAACCCCACCAATGCGGTGCTGGAGCAGCGCGTAGCGGCGCTGGAAGGCGGTATT
GGCGCCATTGCCACCGCCAGCGGACAGGCCGCCCTGCACCTGTCGATTGCCACGCTGATGGGCGCCGGCTCGCACATTGT
TGCCAGCACCGCCCTCTATGGCGGCTCGCAAAACCTGCTGCACTACACGCTGCGCCGCTTCGGCATTGAAACCACCTTTG
TGAAACCCGGCGACCTGGACGGCTGGCGCGCCGCCGTGCGCCCCAACACCAAGCTCCTGTTTGGTGAAACCGTGGGCAAT
CCGGGGCTGGACGTGCTGGACATTTCCGCCGTGGCCGCCATTGCCCATGAAACCGGCGTGCCGCTGCTGGTCGATTCCAC
CCTGACCTCGCCCTGGCTGATCAAGCCTTTCGACCACGGCGCCGACCTGGTGTACCACTCCGCGACCAAGTTTTTGAGCG
GCCACGGCACGGTCATCGGCGGCGTCGTCGTCGACGGCGGCTCGTTTGACTGGGAAAAGTCGGGCAAGTTTGCCGAGTTG
ACCCAAGCCTATGACGGCTTTCACAACATGGTGTTCAGCGAGGAAAGCACCGTGGGCGCCTTTTTGCTGCGCGCGCGCCG
CGAAGGACTGCGCGACTTCGGCGCCTGCATGAGCCCGCACACCGCCTGGCTGATCCTGCAGGGCATTGAAACCCTGCCGC
TGCGCATGGCGCGGCACATGAGCAACACTGAAAAAGTGGTGGACTTCCTGGCCAGCCACCCCTTTGTGGCACGCGTAGGC
CACCCGATGCTGGAATCACACCCCAGCTACGCGCTGGCCAGGCAGTTGCTGCCGCGCGGGGCGGGTTCGGTGTTCAGCTT
TGACCTGAAAGGCAGCCGCGAGCAGGGCGAAACCTTTGTTGAAACCCTCAAGGTCTTCAGCCACCTGGCCAATGTGGGCG
ACTGCCGCAGCCTGGTGATTCACCCGGCCAGTACCACGCACTTCCGGATGACGGATGAAGCGCTGCTCGGCGCCGGCATC
ACGCAGGGCACGATTCGCCTGTCGATCGGTCTGGAGGATCCGGACGATCTGGTGGACGACCTGAAGCGAGCCCTCAAGGC
TGCCGAGAAAGCGGGTGCCTGA

Upstream 100 bases:

>100_bases
TTAAATATAGCCCACAATCAAGCAGCGGGCTGTAACGATTTTCCGGCTTTCGCAGCCAGGTACCGCCCGCAGCCCACGCT
TTTTCTACTGGAGACTTCCC

Downstream 100 bases:

>100_bases
CATGGACATCACCGTCAACGGCCACAAAACCTATTGCTACACCGGTGGTAAAGCGTTTGATGCCGCCAGGCCCACCGTGG
TGTTTGTCCACGGCGTGCTC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSGYSDPGFDTLALHAGAAPDPATGARAVPIYLTTSFVFESSDHAASLFNLERAGHVYSRISNPTNAVLEQRVAALEGGI
GAIATASGQAALHLSIATLMGAGSHIVASTALYGGSQNLLHYTLRRFGIETTFVKPGDLDGWRAAVRPNTKLLFGETVGN
PGLDVLDISAVAAIAHETGVPLLVDSTLTSPWLIKPFDHGADLVYHSATKFLSGHGTVIGGVVVDGGSFDWEKSGKFAEL
TQAYDGFHNMVFSEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMARHMSNTEKVVDFLASHPFVARVG
HPMLESHPSYALARQLLPRGAGSVFSFDLKGSREQGETFVETLKVFSHLANVGDCRSLVIHPASTTHFRMTDEALLGAGI
TQGTIRLSIGLEDPDDLVDDLKRALKAAEKAGA

Sequences:

>Translated_433_residues
MSGYSDPGFDTLALHAGAAPDPATGARAVPIYLTTSFVFESSDHAASLFNLERAGHVYSRISNPTNAVLEQRVAALEGGI
GAIATASGQAALHLSIATLMGAGSHIVASTALYGGSQNLLHYTLRRFGIETTFVKPGDLDGWRAAVRPNTKLLFGETVGN
PGLDVLDISAVAAIAHETGVPLLVDSTLTSPWLIKPFDHGADLVYHSATKFLSGHGTVIGGVVVDGGSFDWEKSGKFAEL
TQAYDGFHNMVFSEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMARHMSNTEKVVDFLASHPFVARVG
HPMLESHPSYALARQLLPRGAGSVFSFDLKGSREQGETFVETLKVFSHLANVGDCRSLVIHPASTTHFRMTDEALLGAGI
TQGTIRLSIGLEDPDDLVDDLKRALKAAEKAGA
>Mature_432_residues
SGYSDPGFDTLALHAGAAPDPATGARAVPIYLTTSFVFESSDHAASLFNLERAGHVYSRISNPTNAVLEQRVAALEGGIG
AIATASGQAALHLSIATLMGAGSHIVASTALYGGSQNLLHYTLRRFGIETTFVKPGDLDGWRAAVRPNTKLLFGETVGNP
GLDVLDISAVAAIAHETGVPLLVDSTLTSPWLIKPFDHGADLVYHSATKFLSGHGTVIGGVVVDGGSFDWEKSGKFAELT
QAYDGFHNMVFSEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMARHMSNTEKVVDFLASHPFVARVGH
PMLESHPSYALARQLLPRGAGSVFSFDLKGSREQGETFVETLKVFSHLANVGDCRSLVIHPASTTHFRMTDEALLGAGIT
QGTIRLSIGLEDPDDLVDDLKRALKAAEKAGA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=35.4609929078014, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=32.6241134751773, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=31.2796208530806, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1790375, Length=421, Percent_Identity=33.729216152019, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1789383, Length=427, Percent_Identity=28.3372365339578, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71980708, Length=404, Percent_Identity=33.1683168316832, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17538055, Length=426, Percent_Identity=33.0985915492958, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17560056, Length=427, Percent_Identity=32.0843091334895, Blast_Score=184, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=42.8240740740741, Blast_Score=314, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6319307, Length=426, Percent_Identity=33.5680751173709, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=447, Percent_Identity=26.6219239373602, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=354, Percent_Identity=25.7062146892655, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=430, Percent_Identity=26.5116279069767, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19922554, Length=428, Percent_Identity=34.8130841121495, Blast_Score=229, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46085; Mature: 45953

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGYSDPGFDTLALHAGAAPDPATGARAVPIYLTTSFVFESSDHAASLFNLERAGHVYSR
CCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHH
ISNPTNAVLEQRVAALEGGIGAIATASGQAALHLSIATLMGAGSHIVASTALYGGSQNLL
HCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEHHHHHCCCCHHHHHHHHCCCCCHHH
HYTLRRFGIETTFVKPGDLDGWRAAVRPNTKLLFGETVGNPGLDVLDISAVAAIAHETGV
HHHHHHHCCCEEEECCCCCCCCEEECCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHCCC
PLLVDSTLTSPWLIKPFDHGADLVYHSATKFLSGHGTVIGGVVVDGGSFDWEKSGKFAEL
CEEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHH
TQAYDGFHNMVFSEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMARHM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHC
SNTEKVVDFLASHPFVARVGHPMLESHPSYALARQLLPRGAGSVFSFDLKGSREQGETFV
CCHHHHHHHHHCCCHHHHCCCHHHHCCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
ETLKVFSHLANVGDCRSLVIHPASTTHFRMTDEALLGAGITQGTIRLSIGLEDPDDLVDD
HHHHHHHHHHCCCCCCEEEEECCCCCEEEECHHHHHCCCCCCCEEEEEECCCCHHHHHHH
LKRALKAAEKAGA
HHHHHHHHHHCCC
>Mature Secondary Structure 
SGYSDPGFDTLALHAGAAPDPATGARAVPIYLTTSFVFESSDHAASLFNLERAGHVYSR
CCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHH
ISNPTNAVLEQRVAALEGGIGAIATASGQAALHLSIATLMGAGSHIVASTALYGGSQNLL
HCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEHHHHHCCCCHHHHHHHHCCCCCHHH
HYTLRRFGIETTFVKPGDLDGWRAAVRPNTKLLFGETVGNPGLDVLDISAVAAIAHETGV
HHHHHHHCCCEEEECCCCCCCCEEECCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHCCC
PLLVDSTLTSPWLIKPFDHGADLVYHSATKFLSGHGTVIGGVVVDGGSFDWEKSGKFAEL
CEEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHH
TQAYDGFHNMVFSEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMARHM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHC
SNTEKVVDFLASHPFVARVGHPMLESHPSYALARQLLPRGAGSVFSFDLKGSREQGETFV
CCHHHHHHHHHCCCHHHHCCCHHHHCCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
ETLKVFSHLANVGDCRSLVIHPASTTHFRMTDEALLGAGITQGTIRLSIGLEDPDDLVDD
HHHHHHHHHHCCCCCCEEEEECCCCCEEEECHHHHHCCCCCCCEEEEEECCCCHHHHHHH
LKRALKAAEKAGA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]