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The map label for this gene is folA [H]

Identifier: 91787701

GI number: 91787701

Start: 1886763

End: 1887257

Strand: Reverse

Name: folA [H]

Synonym: Bpro_1822

Alternate gene names: 91787701

Gene position: 1887257-1886763 (Counterclockwise)

Preceding gene: 91787702

Following gene: 91787700

Centisome position: 36.29

GC content: 58.99

Gene sequence:

>495_bases
ATGCGCATCAACATGATTTTTGCCCGCGCCGCCAACGGCGTGATCGGCAACAACAATCGCATGCCCTGGCATCTGCCGGA
AGACATGGCCCACTTCAAGCGCCTGACCCAGGGCTGGCCCGTCATCATGGGGCGAAAGACCTGGGACTCACTGCCGCCCA
AATTCCGCCCCCTGCCCGGCCGCACCAATATTGTCATCACCCGGCAGCAGGACTGGAAAGAAGATGGCGCCGAGCCAGCA
GCCAGCCTGGCCGACGCTTTGAGCCTTTGCGGGCAGTCTGAAGAAGTCTGGGTCATCGGTGGCGCGCAAATTTACGCGCA
AGCCAAGCCCCTGGCGCAGCGCATCGAAGTCACCGAAATTGCCCAGGACTTTGAAGGGGATGCCTTTGCGCCCCCGCTGG
GTTCCGAATGGATTGAAGCTGCGCGTGAGGATCACATCTCCTCCAATGGGCTGAAGTTCAGTTTTATCACTTACGACAAA
CACAAAGGGAATTGA

Upstream 100 bases:

>100_bases
GCGCAAGCCCGAGTCGATCTTCGACTACCAGTTCGACGATTTCGAGTTCCTTGACTACCAGCACCATGCGGCCATCAAGG
CGCCGGTAGCGGTGTAGTTC

Downstream 100 bases:

>100_bases
CATGTCAGGAGCCGGATTTCACGTCCACGGGCCGCACGACCACGAGCTGGAGCATGCCGCCCAAGGCGCTCATGGCGCAG
CACACGACGGCGGCGGCGGC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MRINMIFARAANGVIGNNNRMPWHLPEDMAHFKRLTQGWPVIMGRKTWDSLPPKFRPLPGRTNIVITRQQDWKEDGAEPA
ASLADALSLCGQSEEVWVIGGAQIYAQAKPLAQRIEVTEIAQDFEGDAFAPPLGSEWIEAAREDHISSNGLKFSFITYDK
HKGN

Sequences:

>Translated_164_residues
MRINMIFARAANGVIGNNNRMPWHLPEDMAHFKRLTQGWPVIMGRKTWDSLPPKFRPLPGRTNIVITRQQDWKEDGAEPA
ASLADALSLCGQSEEVWVIGGAQIYAQAKPLAQRIEVTEIAQDFEGDAFAPPLGSEWIEAAREDHISSNGLKFSFITYDK
HKGN
>Mature_164_residues
MRINMIFARAANGVIGNNNRMPWHLPEDMAHFKRLTQGWPVIMGRKTWDSLPPKFRPLPGRTNIVITRQQDWKEDGAEPA
ASLADALSLCGQSEEVWVIGGAQIYAQAKPLAQRIEVTEIAQDFEGDAFAPPLGSEWIEAAREDHISSNGLKFSFITYDK
HKGN

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=182, Percent_Identity=36.2637362637363, Blast_Score=96, Evalue=1e-20,
Organism=Homo sapiens, GI28827793, Length=146, Percent_Identity=36.986301369863, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1786233, Length=129, Percent_Identity=44.9612403100775, Blast_Score=107, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17505899, Length=178, Percent_Identity=35.9550561797753, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324810, Length=151, Percent_Identity=33.112582781457, Blast_Score=81, Evalue=8e-17,
Organism=Drosophila melanogaster, GI116008026, Length=180, Percent_Identity=31.6666666666667, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24647458, Length=180, Percent_Identity=31.6666666666667, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18371; Mature: 18371

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINMIFARAANGVIGNNNRMPWHLPEDMAHFKRLTQGWPVIMGRKTWDSLPPKFRPLPG
CEEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCC
RTNIVITRQQDWKEDGAEPAASLADALSLCGQSEEVWVIGGAQIYAQAKPLAQRIEVTEI
CCCEEEECCCCCHHCCCCHHHHHHHHHHHCCCCCCEEEEECCEEEEHHHHHHHHHHHHHH
AQDFEGDAFAPPLGSEWIEAAREDHISSNGLKFSFITYDKHKGN
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MRINMIFARAANGVIGNNNRMPWHLPEDMAHFKRLTQGWPVIMGRKTWDSLPPKFRPLPG
CEEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCC
RTNIVITRQQDWKEDGAEPAASLADALSLCGQSEEVWVIGGAQIYAQAKPLAQRIEVTEI
CCCEEEECCCCCHHCCCCHHHHHHHHHHHCCCCCCEEEEECCEEEEHHHHHHHHHHHHHH
AQDFEGDAFAPPLGSEWIEAAREDHISSNGLKFSFITYDKHKGN
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]