Click here to switch to the map view.

The map label for this gene is thyA

Identifier: 91787702

GI number: 91787702

Start: 1887261

End: 1888115

Strand: Reverse

Name: thyA

Synonym: Bpro_1823

Alternate gene names: 91787702

Gene position: 1888115-1887261 (Counterclockwise)

Preceding gene: 91787707

Following gene: 91787701

Centisome position: 36.31

GC content: 60.0

Gene sequence:

>855_bases
ATGAACACCCCTCCCGAAACCCGCCCTGACCGCCCCATTCGTTCCCAGTACGAAGACTTCATGCGCCATGTGGACGCGCA
CGGCGTCTTCAAGGCCGATCGCACCGGCACCGGCACCAAAAGCGTGTTTGGTTACCAGATGCGCTTTGATTTGAACGAAG
GCTTTCCGCTGGTGACCACCAAAAAGGTTCACCTCAAATCCATCGTGCAGGAACTGCTGTGGTTTCTGACCGGGAGCAGC
GACAACAACTGGCTGAAAGAACGCGGCGTGACGATTTGGGATGAGTGGGCCCGCGAAGACGGTGACCTGGGCCCGGTCTA
TGGCGTGCAATGGCGCAGTTGGCCGACGCCTGACGGCGGGCATATTGACCAGATCAGCGAAGTCATCAAGACGCTGAAAA
CCAACCCTGACTCGCGCCGCATCATCGTGAGCGCCTGGAACGTGGCTGACCTGTCCAAAATGGCGCTGATGCCTTGCCAC
GCATTTTTTCAGTTCTACGTCGCGCCTGCCACTGAAGCTGGCGGTAAAGGCAAGCTCAGCTGCCAGCTCTACCAGCGCAG
CGCCGACATTTTCCTCGGCGTGCCCTTCAACATTGGCAGCTACGCGCTGCTCACGCACATGGTGGCGCAGCAGTGCGACC
TGGACGTGGGCGACTTCATCTGGACGGGCGGCGACTGCCACATCTACAGCAACCACGCCGAGCAGGTGGCCCTGCAACTG
AGCCGGGCCCCCTTCCCCTATCCGACACTCCACATCAAGCGCAAGCCCGAGTCGATCTTCGACTACCAGTTCGACGATTT
CGAGTTCCTTGACTACCAGCACCATGCGGCCATCAAGGCGCCGGTAGCGGTGTAG

Upstream 100 bases:

>100_bases
CAGCCAACGCCAGTCCGCATAAAATCTGCCATCCCCGGCCTGGAAAAGTTGCTGCTGCAGCGGGGCAAGGCACTCAAAAT
CATCAAGCTGTTGGCTATTC

Downstream 100 bases:

>100_bases
TTCATGCGCATCAACATGATTTTTGCCCGCGCCGCCAACGGCGTGATCGGCAACAACAATCGCATGCCCTGGCATCTGCC
GGAAGACATGGCCCACTTCA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNTPPETRPDRPIRSQYEDFMRHVDAHGVFKADRTGTGTKSVFGYQMRFDLNEGFPLVTTKKVHLKSIVQELLWFLTGSS
DNNWLKERGVTIWDEWAREDGDLGPVYGVQWRSWPTPDGGHIDQISEVIKTLKTNPDSRRIIVSAWNVADLSKMALMPCH
AFFQFYVAPATEAGGKGKLSCQLYQRSADIFLGVPFNIGSYALLTHMVAQQCDLDVGDFIWTGGDCHIYSNHAEQVALQL
SRAPFPYPTLHIKRKPESIFDYQFDDFEFLDYQHHAAIKAPVAV

Sequences:

>Translated_284_residues
MNTPPETRPDRPIRSQYEDFMRHVDAHGVFKADRTGTGTKSVFGYQMRFDLNEGFPLVTTKKVHLKSIVQELLWFLTGSS
DNNWLKERGVTIWDEWAREDGDLGPVYGVQWRSWPTPDGGHIDQISEVIKTLKTNPDSRRIIVSAWNVADLSKMALMPCH
AFFQFYVAPATEAGGKGKLSCQLYQRSADIFLGVPFNIGSYALLTHMVAQQCDLDVGDFIWTGGDCHIYSNHAEQVALQL
SRAPFPYPTLHIKRKPESIFDYQFDDFEFLDYQHHAAIKAPVAV
>Mature_284_residues
MNTPPETRPDRPIRSQYEDFMRHVDAHGVFKADRTGTGTKSVFGYQMRFDLNEGFPLVTTKKVHLKSIVQELLWFLTGSS
DNNWLKERGVTIWDEWAREDGDLGPVYGVQWRSWPTPDGGHIDQISEVIKTLKTNPDSRRIIVSAWNVADLSKMALMPCH
AFFQFYVAPATEAGGKGKLSCQLYQRSADIFLGVPFNIGSYALLTHMVAQQCDLDVGDFIWTGGDCHIYSNHAEQVALQL
SRAPFPYPTLHIKRKPESIFDYQFDDFEFLDYQHHAAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=50.1730103806228, Blast_Score=270, Evalue=8e-73,
Organism=Escherichia coli, GI1789191, Length=270, Percent_Identity=68.1481481481482, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71993377, Length=290, Percent_Identity=45.1724137931035, Blast_Score=250, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6324648, Length=228, Percent_Identity=50, Blast_Score=207, Evalue=1e-54,
Organism=Drosophila melanogaster, GI17137556, Length=288, Percent_Identity=49.6527777777778, Blast_Score=278, Evalue=3e-75,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_POLSJ (Q12CI6)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548654.1
- ProteinModelPortal:   Q12CI6
- SMR:   Q12CI6
- STRING:   Q12CI6
- GeneID:   4015533
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1823
- NMPDR:   fig|296591.1.peg.2781
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q12CI6
- ProtClustDB:   PRK01827
- BioCyc:   PSP296591:BPRO_1823-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32301; Mature: 32301

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPPETRPDRPIRSQYEDFMRHVDAHGVFKADRTGTGTKSVFGYQMRFDLNEGFPLVTT
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEECCCCCCEEEE
KKVHLKSIVQELLWFLTGSSDNNWLKERGVTIWDEWAREDGDLGPVYGVQWRSWPTPDGG
CHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEHHHCCCCCCCCCEECCCCCCCCCCCCC
HIDQISEVIKTLKTNPDSRRIIVSAWNVADLSKMALMPCHAFFQFYVAPATEAGGKGKLS
CHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
CQLYQRSADIFLGVPFNIGSYALLTHMVAQQCDLDVGDFIWTGGDCHIYSNHAEQVALQL
EEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCEEEEECCHHHHHHHHH
SRAPFPYPTLHIKRKPESIFDYQFDDFEFLDYQHHAAIKAPVAV
HCCCCCCCEEEEEECCCHHHCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNTPPETRPDRPIRSQYEDFMRHVDAHGVFKADRTGTGTKSVFGYQMRFDLNEGFPLVTT
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEECCCCCCEEEE
KKVHLKSIVQELLWFLTGSSDNNWLKERGVTIWDEWAREDGDLGPVYGVQWRSWPTPDGG
CHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEHHHCCCCCCCCCEECCCCCCCCCCCCC
HIDQISEVIKTLKTNPDSRRIIVSAWNVADLSKMALMPCHAFFQFYVAPATEAGGKGKLS
CHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
CQLYQRSADIFLGVPFNIGSYALLTHMVAQQCDLDVGDFIWTGGDCHIYSNHAEQVALQL
EEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCEEEEECCHHHHHHHHH
SRAPFPYPTLHIKRKPESIFDYQFDDFEFLDYQHHAAIKAPVAV
HCCCCCCCEEEEEECCCHHHCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA