| Definition | Polaromonas sp. JS666 chromosome, complete genome. |
|---|---|
| Accession | NC_007948 |
| Length | 5,200,264 |
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The map label for this gene is murF [H]
Identifier: 91786969
GI number: 91786969
Start: 1102067
End: 1103446
Strand: Direct
Name: murF [H]
Synonym: Bpro_1071
Alternate gene names: 91786969
Gene position: 1102067-1103446 (Clockwise)
Preceding gene: 91786968
Following gene: 91786970
Centisome position: 21.19
GC content: 65.8
Gene sequence:
>1380_bases ATGATGACCTTGCAACAAGCCCTGGGCTGGTTGCCGTCGGCCCGGCTAGTGGGTGATGGCAGCGTGAAGCCTCTGCGCGT GCACACCGATACCCGCAGCCTGCAGGCCGGCGACCTGTTCGTGGCGCTCAAGGGCGAGCGCTTTGACGCCCATGATTTTC TGGCGCAGGCCAAGGCACAGGGCGCCGTGGCGGCGATTGCCCACCATGGCCTGGCCGCGGCAGGTCTGCCGGGTCTGGAG GTGGCTGACTCGAAACTGGCGCTCGGCCAACTGGCGGCCGGCTGGCGTTCGCAATTCAGCTTGCCCCTCATTGCGGTGAC GGGAAGCAACGGAAAAACGACGGTGACGCAGATGATCGCGGCTATCCTGCGGGCCTGGAAATCCACGCAGGCTTTTGCCA CGGAAGGCAATCTCAACAACGACATCGGCGTGCCCCTGACGCTGTTGCGCCTTCGTGCCTCCCATGCGGTGGGCGTGGTG GAGCTGGGCATGAACCATCCCGGCGAAATCGCCTACCTGGCCGCCTTGGCCAAGCCTGGCGTGGCGCTGGTCAATAACGC CCAGCGCGAGCACCTTGAATTCATGGCCACGGTGGAAGCCGTCGCCCGGGAGAACGGCTCCGTGCTGCAGGCCCTCGGCA CGGATGGCATTGCCGTTTTTCCTGCCGATGATGACTACACCGCCATCTGGCGCGGCCTCGCGGGGGCGCGCCGCGTACTG ACTTTTGCACAAACGGGCCCGGCCGATGTCACGGCCCAGGCGCAGTGGAGCGGCGACTGCTGGCAGGTTGCGGCCCGGAC ACCCGCAGGGCCGCTTGATTTTCCCCTGCATATTGCGGGCCGGCACAACGTCAAGAATGCGCTGGCCGCCACCGCCTGCG CGCTGGCCGCCGGCGTTCCCCTGCCAGTCATCGCGGCGGGTCTATCGGAATTTGCCCCGGTCAAGGGACGTTCCCGCGCC CTGACCGTGCGCCTGGCGAATCGTGTGGTGACATTGATCGACGACACCTACAACGCCAATCCCGACTCCATGCGCGCGGC CATCGAGGTGTTGGCCGAGCTGCCGGCGCCGCGCCTGCTGGTCATGGGCGACATGGGTGAGGTGGGCGACCAGGGTCCCC AGTTTCACGAAGAGGCCGGCCGGTATGCCCACGCGCGGGGCATCGAGCAGCTGTTCACGCTGGGAGCCCAGTCCCAAAGG GCGAGTACCGCTTTCGGCGCGGGACGCCATTTCAGCCAGATGGCCGATCTGGCCGCCGCCGTGGTGGCTGAATTGCCGCA GGCGGGCAGTGTGCTGGTGAAGGGCTCCCGCTTCATGAAGATGGAGCAGGTGGTTCAGGTCATCGCAGATCACACAAAAA ACTACAAGGAAGAAACATGA
Upstream 100 bases:
>100_bases TCCAGGGCGTGAAGCTTCCTTTTTCCGACAGGACGCAGGCGCAAGCCGCTCTCGCCCTGAATGCGGCGCGCGTGGCACGC CAGGCCGGGGGAGGCTCCGC
Downstream 100 bases:
>100_bases GCCCCCATGCTCACATGCGTTCGCTGTCCCATCAGGGGGGGCATGCCTCCTTCGAGGCGGTTCGGCAGGAGGCATTTCAT GCTGCTTAGCCTGGCCCAGT
Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 459; Mature: 459
Protein sequence:
>459_residues MMTLQQALGWLPSARLVGDGSVKPLRVHTDTRSLQAGDLFVALKGERFDAHDFLAQAKAQGAVAAIAHHGLAAAGLPGLE VADSKLALGQLAAGWRSQFSLPLIAVTGSNGKTTVTQMIAAILRAWKSTQAFATEGNLNNDIGVPLTLLRLRASHAVGVV ELGMNHPGEIAYLAALAKPGVALVNNAQREHLEFMATVEAVARENGSVLQALGTDGIAVFPADDDYTAIWRGLAGARRVL TFAQTGPADVTAQAQWSGDCWQVAARTPAGPLDFPLHIAGRHNVKNALAATACALAAGVPLPVIAAGLSEFAPVKGRSRA LTVRLANRVVTLIDDTYNANPDSMRAAIEVLAELPAPRLLVMGDMGEVGDQGPQFHEEAGRYAHARGIEQLFTLGAQSQR ASTAFGAGRHFSQMADLAAAVVAELPQAGSVLVKGSRFMKMEQVVQVIADHTKNYKEET
Sequences:
>Translated_459_residues MMTLQQALGWLPSARLVGDGSVKPLRVHTDTRSLQAGDLFVALKGERFDAHDFLAQAKAQGAVAAIAHHGLAAAGLPGLE VADSKLALGQLAAGWRSQFSLPLIAVTGSNGKTTVTQMIAAILRAWKSTQAFATEGNLNNDIGVPLTLLRLRASHAVGVV ELGMNHPGEIAYLAALAKPGVALVNNAQREHLEFMATVEAVARENGSVLQALGTDGIAVFPADDDYTAIWRGLAGARRVL TFAQTGPADVTAQAQWSGDCWQVAARTPAGPLDFPLHIAGRHNVKNALAATACALAAGVPLPVIAAGLSEFAPVKGRSRA LTVRLANRVVTLIDDTYNANPDSMRAAIEVLAELPAPRLLVMGDMGEVGDQGPQFHEEAGRYAHARGIEQLFTLGAQSQR ASTAFGAGRHFSQMADLAAAVVAELPQAGSVLVKGSRFMKMEQVVQVIADHTKNYKEET >Mature_459_residues MMTLQQALGWLPSARLVGDGSVKPLRVHTDTRSLQAGDLFVALKGERFDAHDFLAQAKAQGAVAAIAHHGLAAAGLPGLE VADSKLALGQLAAGWRSQFSLPLIAVTGSNGKTTVTQMIAAILRAWKSTQAFATEGNLNNDIGVPLTLLRLRASHAVGVV ELGMNHPGEIAYLAALAKPGVALVNNAQREHLEFMATVEAVARENGSVLQALGTDGIAVFPADDDYTAIWRGLAGARRVL TFAQTGPADVTAQAQWSGDCWQVAARTPAGPLDFPLHIAGRHNVKNALAATACALAAGVPLPVIAAGLSEFAPVKGRSRA LTVRLANRVVTLIDDTYNANPDSMRAAIEVLAELPAPRLLVMGDMGEVGDQGPQFHEEAGRYAHARGIEQLFTLGAQSQR ASTAFGAGRHFSQMADLAAAVVAELPQAGSVLVKGSRFMKMEQVVQVIADHTKNYKEET
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=430, Percent_Identity=44.1860465116279, Blast_Score=290, Evalue=2e-79,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 48278; Mature: 48278
Theoretical pI: Translated: 7.21; Mature: 7.21
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMTLQQALGWLPSARLVGDGSVKPLRVHTDTRSLQAGDLFVALKGERFDAHDFLAQAKAQ CCCHHHHHCCCCCCEEECCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHC GAVAAIAHHGLAAAGLPGLEVADSKLALGQLAAGWRSQFSLPLIAVTGSNGKTTVTQMIA CHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH AILRAWKSTQAFATEGNLNNDIGVPLTLLRLRASHAVGVVELGMNHPGEIAYLAALAKPG HHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCC VALVNNAQREHLEFMATVEAVARENGSVLQALGTDGIAVFPADDDYTAIWRGLAGARRVL HHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHE TFAQTGPADVTAQAQWSGDCWQVAARTPAGPLDFPLHIAGRHNVKNALAATACALAAGVP EEECCCCCCEEEEEECCCCHHEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCC LPVIAAGLSEFAPVKGRSRALTVRLANRVVTLIDDTYNANPDSMRAAIEVLAELPAPRLL HHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEE VMGDMGEVGDQGPQFHEEAGRYAHARGIEQLFTLGAQSQRASTAFGAGRHFSQMADLAAA EEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHH VVAELPQAGSVLVKGSRFMKMEQVVQVIADHTKNYKEET HHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCC >Mature Secondary Structure MMTLQQALGWLPSARLVGDGSVKPLRVHTDTRSLQAGDLFVALKGERFDAHDFLAQAKAQ CCCHHHHHCCCCCCEEECCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHC GAVAAIAHHGLAAAGLPGLEVADSKLALGQLAAGWRSQFSLPLIAVTGSNGKTTVTQMIA CHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH AILRAWKSTQAFATEGNLNNDIGVPLTLLRLRASHAVGVVELGMNHPGEIAYLAALAKPG HHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCC VALVNNAQREHLEFMATVEAVARENGSVLQALGTDGIAVFPADDDYTAIWRGLAGARRVL HHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHE TFAQTGPADVTAQAQWSGDCWQVAARTPAGPLDFPLHIAGRHNVKNALAATACALAAGVP EEECCCCCCEEEEEECCCCHHEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCC LPVIAAGLSEFAPVKGRSRALTVRLANRVVTLIDDTYNANPDSMRAAIEVLAELPAPRLL HHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEE VMGDMGEVGDQGPQFHEEAGRYAHARGIEQLFTLGAQSQRASTAFGAGRHFSQMADLAAA EEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHH VVAELPQAGSVLVKGSRFMKMEQVVQVIADHTKNYKEET HHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]