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The map label for this gene is vanB [H]

Identifier: 91786895

GI number: 91786895

Start: 1011782

End: 1012741

Strand: Direct

Name: vanB [H]

Synonym: Bpro_0995

Alternate gene names: 91786895

Gene position: 1011782-1012741 (Clockwise)

Preceding gene: 91786894

Following gene: 91786898

Centisome position: 19.46

GC content: 59.38

Gene sequence:

>960_bases
ATGAGTGCCAATGAGATTGCTGTAAAAGTCGCGAGGAAGGTTCGCGAGGCGCAGGACATCAATTCCTATGAATTGGTGTC
CGTCAATGGGCAGGCGCTTCCCGCGTTTGAAGCGGGTGCGCATGTGGATGTGCATCTCCCCAATGGCCTCGTGCGGCAAT
ACTCCCTGTGCGGAATGCCGCACCAGAAGGACCGTTATCGCATCGCGGTGCTCAGGGACCCGAAATCCCGTGGAGGGTCC
GAGGCGGTCCATGACCTGGTGCACGAAGGCGATACCCTGCGCATCAGTGCGCCGCGTAATCTCTTCAGGCTGGCGGCGGG
GGAGCAAACCAGCGTTCTGCTGGCAGGCGGTATCGGCGTGACGCCTTTGCTGGCGATGGCATATCACCTGCACGCGCTGG
GCAGCGCCTTTGCGCTGCACTATTTCGCGCGATCCAGAGACCGCGTGGCATTCCTTGCCGAACTCCAGAACAGCGGCTTT
GCCAGAAATGTGGTGTTGCACCTGGATGACGAGGCTGTCGCGGGCGCCAGGCCCTTACGTTCACTTCTCGAAACCCTGCA
GCGCGATGCGCATATTTACACCTGTGGCCCTACTGGTTTCCTGAACCACGTTCTGGAAACGGCCTCCGCGCTGGATTGGC
CCGGCGGCCAGGTGCACTATGAGACCTTTTCGCCGCCGCAGCCGACAGCCGGCGATTCCTTCGAGGTTCGCATTTCGAGC
ACCGGACAGTCTGTAGTGGTCGCTGCCGATGAGTCGGTGGTCAGCGCGGTCGCACGGCTTGGCATAGAGATTCCGGTTTC
ATGTGAGCAAGGTATTTGCGGAACCTGCCTGACACGCGTGGCAGAAGGCGTTCCGGATCACCATGATCAATACCTGACAA
ACGAAGAACACGAGAGAAACGATCAATTCACCCCATGCTGCTCAAGAGCCCGATCGAAGGTGCTGGTCCTGGATTTATAG

Upstream 100 bases:

>100_bases
GATGGCAGCGGCCGCCTGAACCTGAAGTCGGTTTCCACGCCGTTCAAGAGCTACCAGGATGCCTACGCGTCCCAATGTCC
CCTGAAGTGATGTGCAGGTC

Downstream 100 bases:

>100_bases
CTTTTCATTTACAAATTCCGAGGCGTTCGTCGTGTGCTTCGTGTACGTTTCGCAGTCCGAAGTGACGCGATAGCTCAGGC
GGGTCGCCTGGTCGCGGCTT

Product: ferredoxin

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSANEIAVKVARKVREAQDINSYELVSVNGQALPAFEAGAHVDVHLPNGLVRQYSLCGMPHQKDRYRIAVLRDPKSRGGS
EAVHDLVHEGDTLRISAPRNLFRLAAGEQTSVLLAGGIGVTPLLAMAYHLHALGSAFALHYFARSRDRVAFLAELQNSGF
ARNVVLHLDDEAVAGARPLRSLLETLQRDAHIYTCGPTGFLNHVLETASALDWPGGQVHYETFSPPQPTAGDSFEVRISS
TGQSVVVAADESVVSAVARLGIEIPVSCEQGICGTCLTRVAEGVPDHHDQYLTNEEHERNDQFTPCCSRARSKVLVLDL

Sequences:

>Translated_319_residues
MSANEIAVKVARKVREAQDINSYELVSVNGQALPAFEAGAHVDVHLPNGLVRQYSLCGMPHQKDRYRIAVLRDPKSRGGS
EAVHDLVHEGDTLRISAPRNLFRLAAGEQTSVLLAGGIGVTPLLAMAYHLHALGSAFALHYFARSRDRVAFLAELQNSGF
ARNVVLHLDDEAVAGARPLRSLLETLQRDAHIYTCGPTGFLNHVLETASALDWPGGQVHYETFSPPQPTAGDSFEVRISS
TGQSVVVAADESVVSAVARLGIEIPVSCEQGICGTCLTRVAEGVPDHHDQYLTNEEHERNDQFTPCCSRARSKVLVLDL
>Mature_318_residues
SANEIAVKVARKVREAQDINSYELVSVNGQALPAFEAGAHVDVHLPNGLVRQYSLCGMPHQKDRYRIAVLRDPKSRGGSE
AVHDLVHEGDTLRISAPRNLFRLAAGEQTSVLLAGGIGVTPLLAMAYHLHALGSAFALHYFARSRDRVAFLAELQNSGFA
RNVVLHLDDEAVAGARPLRSLLETLQRDAHIYTCGPTGFLNHVLETASALDWPGGQVHYETFSPPQPTAGDSFEVRISST
GQSVVVAADESVVSAVARLGIEIPVSCEQGICGTCLTRVAEGVPDHHDQYLTNEEHERNDQFTPCCSRARSKVLVLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=317, Percent_Identity=35.6466876971609, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1788903, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787658, Length=229, Percent_Identity=29.2576419213974, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 34588; Mature: 34457

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANEIAVKVARKVREAQDINSYELVSVNGQALPAFEAGAHVDVHLPNGLVRQYSLCGMP
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHCCCC
HQKDRYRIAVLRDPKSRGGSEAVHDLVHEGDTLRISAPRNLFRLAAGEQTSVLLAGGIGV
CCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCEEEEECCCCH
TPLLAMAYHLHALGSAFALHYFARSRDRVAFLAELQNSGFARNVVLHLDDEAVAGARPLR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCCHHCCHHHHH
SLLETLQRDAHIYTCGPTGFLNHVLETASALDWPGGQVHYETFSPPQPTAGDSFEVRISS
HHHHHHHHCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEEC
TGQSVVVAADESVVSAVARLGIEIPVSCEQGICGTCLTRVAEGVPDHHDQYLTNEEHERN
CCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCHHHCC
DQFTPCCSRARSKVLVLDL
CCCCHHHHHCCCCEEEEEC
>Mature Secondary Structure 
SANEIAVKVARKVREAQDINSYELVSVNGQALPAFEAGAHVDVHLPNGLVRQYSLCGMP
CCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHCCCC
HQKDRYRIAVLRDPKSRGGSEAVHDLVHEGDTLRISAPRNLFRLAAGEQTSVLLAGGIGV
CCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCEEEEECCCCH
TPLLAMAYHLHALGSAFALHYFARSRDRVAFLAELQNSGFARNVVLHLDDEAVAGARPLR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCCHHCCHHHHH
SLLETLQRDAHIYTCGPTGFLNHVLETASALDWPGGQVHYETFSPPQPTAGDSFEVRISS
HHHHHHHHCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEEC
TGQSVVVAADESVVSAVARLGIEIPVSCEQGICGTCLTRVAEGVPDHHDQYLTNEEHERN
CCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCHHHCC
DQFTPCCSRARSKVLVLDL
CCCCHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579070 [H]