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The map label for this gene is hycE [H]

Identifier: 91786822

GI number: 91786822

Start: 937285

End: 938835

Strand: Direct

Name: hycE [H]

Synonym: Bpro_0920

Alternate gene names: 91786822

Gene position: 937285-938835 (Clockwise)

Preceding gene: 91786821

Following gene: 91786823

Centisome position: 18.02

GC content: 68.99

Gene sequence:

>1551_bases
ATGGCGCTGCCTGACCTTGGACTCGACCTGCGGCGCCTGCCGGCGCCCCTGCCTATGTGGCACGGACAGGTCGGTCGCGA
CGCCTGGAGCTCCGCGGCGCGCACCATCGCGGACAGCGGCGGGCGACTGGTTTCAGTTTGGGGCGTGGACCGCCGGGCCA
GTGGCGGCGCCATGGCGGCCTGTGCGGCCTATGCTGTATTCGAGGGGCTGGTCTGGCTCGAACTGCCGCTCGACGATGCG
ACACCGGGCTTTCCGGATCTGTCGTCCGCGTTCCCGTGTGCGGGCCGGATGCAGCGCGCCATGGCCGACCTGTCGGGCCT
GCACGCAGACGGCAGCCGTGACGACCGCCCGTGGCTGGACCATGGCATGTGGGCGCCCGGAAACCCACCGCTGCAACACC
ATGCCGAACCGAAGGCTGCGGGCGTCGGCACGCTGCCCGCCGATTACCCCTTCGTGTGCGTCGAAGGCGACGGGGTGCAC
GAGATCGCCGTCGGCCCGGTGCATGCCGGCATCATCGAGCCTGGCCACTTCCGTTTCTCGGTGGTCGGCGAGAAGGTACT
CCGGCTGGAAGAGCATCTGGGCTACACGCACAAGGGCATCGAGCGCCGCTTCACCGAACTGACGCCAATCGAAGGCCACC
GGCTCGCTGGCCGCGTGTCGGGCGACTCGACGGTGGCGTATGCCTGGGCCTATTGCATGGCGCTCGAGTCGGCTTGGGGC
AGCAGGATCCCGGCCAGGGCGACCTGGCTGCGCGCGCTGATGCTGGAGCGCGAGCGCGTGGCCAACCACCTGGGTGACCT
TGGCGCCCTGGGTAACGACGCGGCGCTGGCCTTCGGTCTGGCGCAGTTCTCGCGCCTGCGCGAGGACTGGCAGCGCCTGT
CCAAAGAGGCCTTCGGCCACCGCTTCATGATGGACGCGGTGGTACCTGGAGGCGTCGCGGTCGACCTCGCGCCACCGATG
CTCGATCGCCTGCGCAAGCAGTGCGACGACATCGAGCGGGACGTGCGCGCGCTGAAGGTCGTGTACGACGAACATGCGGG
CCTGCAGGACCGCTTGCTCACCACCGGGAGGGTGACCCCTCAGCTCGCCGCCCAGCTCGGTCTCACGGGTCTGGCAGGGC
GCGCCAGCGGTCAGACGGCCGACCTGCGCTGCGACCACGAATGGGCGCCCTACGACCGGTTCGAGGTCGCGATGGCCACA
CACCGCAATGGCGATGTCGCAGCGCGCGTGACGGTGCGCTTCGAAGAGACGTTCGAGTCGTTGAGGCTCATCCGCGCCAT
TTGCGCCGGCTTGCCGGGCGATGCCACCCATGTCGACCTGCGGCCACCGGCTGTGCCGACCATGGGTGCGGGCTGGGTGG
AGGGCTGGCGCGGCGAGGTTTTCGTCGCGCTCGAGATTGGGGGCGATGGCCGCGTCGTGCGCTGCCACTGTCACGACCCG
TCGTGGCAGAACTGGCCGGTGCTCGAGCACGCCATCATCGGCAACATCGTTCCGGACTTTCCGCTGATCAACAAGTCCTT
CAATCTCAGCTATTCGGGGCACGACCTCTAG

Upstream 100 bases:

>100_bases
TTTGTTCACCTGGCGCTCGGGCTGATGCTGGGCCTGTACATTCCGTCCTATCTCAACGCCTGGTACCGGCAGGCGGCCGC
CATGCTGGGAAGCTGACCGT

Downstream 100 bases:

>100_bases
AGATCCCTGACATGTGGCACATCCTCAAACAGATCGCCCGCACCGGCATCATCACCGAACCGGCTCCGGGTATTGACGAC
GCCCAACGTACCGCGATCGA

Product: NADH-ubiquinone oxidoreductase subunit 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MALPDLGLDLRRLPAPLPMWHGQVGRDAWSSAARTIADSGGRLVSVWGVDRRASGGAMAACAAYAVFEGLVWLELPLDDA
TPGFPDLSSAFPCAGRMQRAMADLSGLHADGSRDDRPWLDHGMWAPGNPPLQHHAEPKAAGVGTLPADYPFVCVEGDGVH
EIAVGPVHAGIIEPGHFRFSVVGEKVLRLEEHLGYTHKGIERRFTELTPIEGHRLAGRVSGDSTVAYAWAYCMALESAWG
SRIPARATWLRALMLERERVANHLGDLGALGNDAALAFGLAQFSRLREDWQRLSKEAFGHRFMMDAVVPGGVAVDLAPPM
LDRLRKQCDDIERDVRALKVVYDEHAGLQDRLLTTGRVTPQLAAQLGLTGLAGRASGQTADLRCDHEWAPYDRFEVAMAT
HRNGDVAARVTVRFEETFESLRLIRAICAGLPGDATHVDLRPPAVPTMGAGWVEGWRGEVFVALEIGGDGRVVRCHCHDP
SWQNWPVLEHAIIGNIVPDFPLINKSFNLSYSGHDL

Sequences:

>Translated_516_residues
MALPDLGLDLRRLPAPLPMWHGQVGRDAWSSAARTIADSGGRLVSVWGVDRRASGGAMAACAAYAVFEGLVWLELPLDDA
TPGFPDLSSAFPCAGRMQRAMADLSGLHADGSRDDRPWLDHGMWAPGNPPLQHHAEPKAAGVGTLPADYPFVCVEGDGVH
EIAVGPVHAGIIEPGHFRFSVVGEKVLRLEEHLGYTHKGIERRFTELTPIEGHRLAGRVSGDSTVAYAWAYCMALESAWG
SRIPARATWLRALMLERERVANHLGDLGALGNDAALAFGLAQFSRLREDWQRLSKEAFGHRFMMDAVVPGGVAVDLAPPM
LDRLRKQCDDIERDVRALKVVYDEHAGLQDRLLTTGRVTPQLAAQLGLTGLAGRASGQTADLRCDHEWAPYDRFEVAMAT
HRNGDVAARVTVRFEETFESLRLIRAICAGLPGDATHVDLRPPAVPTMGAGWVEGWRGEVFVALEIGGDGRVVRCHCHDP
SWQNWPVLEHAIIGNIVPDFPLINKSFNLSYSGHDL
>Mature_515_residues
ALPDLGLDLRRLPAPLPMWHGQVGRDAWSSAARTIADSGGRLVSVWGVDRRASGGAMAACAAYAVFEGLVWLELPLDDAT
PGFPDLSSAFPCAGRMQRAMADLSGLHADGSRDDRPWLDHGMWAPGNPPLQHHAEPKAAGVGTLPADYPFVCVEGDGVHE
IAVGPVHAGIIEPGHFRFSVVGEKVLRLEEHLGYTHKGIERRFTELTPIEGHRLAGRVSGDSTVAYAWAYCMALESAWGS
RIPARATWLRALMLERERVANHLGDLGALGNDAALAFGLAQFSRLREDWQRLSKEAFGHRFMMDAVVPGGVAVDLAPPML
DRLRKQCDDIERDVRALKVVYDEHAGLQDRLLTTGRVTPQLAAQLGLTGLAGRASGQTADLRCDHEWAPYDRFEVAMATH
RNGDVAARVTVRFEETFESLRLIRAICAGLPGDATHVDLRPPAVPTMGAGWVEGWRGEVFVALEIGGDGRVVRCHCHDPS
WQNWPVLEHAIIGNIVPDFPLINKSFNLSYSGHDL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=372, Percent_Identity=24.7311827956989, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI260898743, Length=372, Percent_Identity=24.7311827956989, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1789076, Length=484, Percent_Identity=28.5123966942149, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1788832, Length=487, Percent_Identity=29.5687885010267, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17568379, Length=360, Percent_Identity=23.0555555555556, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17555284, Length=360, Percent_Identity=22.2222222222222, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221459469, Length=381, Percent_Identity=24.1469816272966, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24638644, Length=336, Percent_Identity=24.702380952381, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 56246; Mature: 56114

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPDLGLDLRRLPAPLPMWHGQVGRDAWSSAARTIADSGGRLVSVWGVDRRASGGAMAA
CCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
CAAYAVFEGLVWLELPLDDATPGFPDLSSAFPCAGRMQRAMADLSGLHADGSRDDRPWLD
HHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
HGMWAPGNPPLQHHAEPKAAGVGTLPADYPFVCVEGDGVHEIAVGPVHAGIIEPGHFRFS
CCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCEECCCCCCCCEEEE
VVGEKVLRLEEHLGYTHKGIERRFTELTPIEGHRLAGRVSGDSTVAYAWAYCMALESAWG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
SRIPARATWLRALMLERERVANHLGDLGALGNDAALAFGLAQFSRLREDWQRLSKEAFGH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RFMMDAVVPGGVAVDLAPPMLDRLRKQCDDIERDVRALKVVYDEHAGLQDRLLTTGRVTP
CEEHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCH
QLAAQLGLTGLAGRASGQTADLRCDHEWAPYDRFEVAMATHRNGDVAARVTVRFEETFES
HHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCEEEEEEEEHHHHHHH
LRLIRAICAGLPGDATHVDLRPPAVPTMGAGWVEGWRGEVFVALEIGGDGRVVRCHCHDP
HHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCEEEEEECCC
SWQNWPVLEHAIIGNIVPDFPLINKSFNLSYSGHDL
CCCCCCHHHHHHHHCCCCCCCCCCCCEECCCCCCCC
>Mature Secondary Structure 
ALPDLGLDLRRLPAPLPMWHGQVGRDAWSSAARTIADSGGRLVSVWGVDRRASGGAMAA
CCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
CAAYAVFEGLVWLELPLDDATPGFPDLSSAFPCAGRMQRAMADLSGLHADGSRDDRPWLD
HHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
HGMWAPGNPPLQHHAEPKAAGVGTLPADYPFVCVEGDGVHEIAVGPVHAGIIEPGHFRFS
CCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCEECCCCCCCCEEEE
VVGEKVLRLEEHLGYTHKGIERRFTELTPIEGHRLAGRVSGDSTVAYAWAYCMALESAWG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
SRIPARATWLRALMLERERVANHLGDLGALGNDAALAFGLAQFSRLREDWQRLSKEAFGH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RFMMDAVVPGGVAVDLAPPMLDRLRKQCDDIERDVRALKVVYDEHAGLQDRLLTTGRVTP
CEEHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCH
QLAAQLGLTGLAGRASGQTADLRCDHEWAPYDRFEVAMATHRNGDVAARVTVRFEETFES
HHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCEEEEEEEEHHHHHHH
LRLIRAICAGLPGDATHVDLRPPAVPTMGAGWVEGWRGEVFVALEIGGDGRVVRCHCHDP
HHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCEEEEEECCC
SWQNWPVLEHAIIGNIVPDFPLINKSFNLSYSGHDL
CCCCCCHHHHHHHHCCCCCCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]