Click here to switch to the map view.

The map label for this gene is hycG [H]

Identifier: 91786823

GI number: 91786823

Start: 938847

End: 939392

Strand: Direct

Name: hycG [H]

Synonym: Bpro_0921

Alternate gene names: 91786823

Gene position: 938847-939392 (Clockwise)

Preceding gene: 91786822

Following gene: 91786825

Centisome position: 18.05

GC content: 64.29

Gene sequence:

>546_bases
ATGTGGCACATCCTCAAACAGATCGCCCGCACCGGCATCATCACCGAACCGGCTCCGGGTATTGACGACGCCCAACGTAC
CGCGATCGAGCGCATCCACCGCGATATTTTGCTGATCCTCGGTCAGGCGCTGACCATCCGCGCGGTCGATGCCGGCTCGT
GCAATGGTTGCGAGCTGGAAATCCACGCGCTCAACAACCCCTACTACAACATCGAAGGGCTGGGCATCAAGTTCGTCGCC
AGCCCACGCCACGCTGACATGCTGCTGGTGACCGGCCCGGTATCGCGCCACATGGACGAGGCGTTGCGGCGCACCTATGA
CGCGACGCCCGAGCCGAAACTCGTGGTCGCTGTGGGGGACTGCGGCTGCACCGGTGGTATTTTCGGCGACAGCTACGCGA
GCTGTGGCCGCGTTGCCAACGTGATTCCTGTGGATGTCACCGTGCCTGGCTGCCCGCCGCCGCCCATAGAAATTCTGCGG
GGAATACTGACCGCGGTGCGGCAGCGCCGACCCGGCACGGCGGGAGATAACCCTGCAGGCAGCTGA

Upstream 100 bases:

>100_bases
TCGAGCACGCCATCATCGGCAACATCGTTCCGGACTTTCCGCTGATCAACAAGTCCTTCAATCTCAGCTATTCGGGGCAC
GACCTCTAGAGATCCCTGAC

Downstream 100 bases:

>100_bases
CGGGTTCTTGCTGTGCTGTATGGACCGGAGACATGGGACATGGGACATGGGACAGTGGACAGTGGACAGTGGACAGTGGA
CAGTGGACAGGGGACAGGGG

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MWHILKQIARTGIITEPAPGIDDAQRTAIERIHRDILLILGQALTIRAVDAGSCNGCELEIHALNNPYYNIEGLGIKFVA
SPRHADMLLVTGPVSRHMDEALRRTYDATPEPKLVVAVGDCGCTGGIFGDSYASCGRVANVIPVDVTVPGCPPPPIEILR
GILTAVRQRRPGTAGDNPAGS

Sequences:

>Translated_181_residues
MWHILKQIARTGIITEPAPGIDDAQRTAIERIHRDILLILGQALTIRAVDAGSCNGCELEIHALNNPYYNIEGLGIKFVA
SPRHADMLLVTGPVSRHMDEALRRTYDATPEPKLVVAVGDCGCTGGIFGDSYASCGRVANVIPVDVTVPGCPPPPIEILR
GILTAVRQRRPGTAGDNPAGS
>Mature_181_residues
MWHILKQIARTGIITEPAPGIDDAQRTAIERIHRDILLILGQALTIRAVDAGSCNGCELEIHALNNPYYNIEGLGIKFVA
SPRHADMLLVTGPVSRHMDEALRRTYDATPEPKLVVAVGDCGCTGGIFGDSYASCGRVANVIPVDVTVPGCPPPPIEILR
GILTAVRQRRPGTAGDNPAGS

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=112, Percent_Identity=38.3928571428571, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1789074, Length=159, Percent_Identity=40.251572327044, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788834, Length=137, Percent_Identity=41.6058394160584, Blast_Score=120, Evalue=4e-29,
Organism=Escherichia coli, GI1788624, Length=124, Percent_Identity=33.8709677419355, Blast_Score=74, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17509685, Length=153, Percent_Identity=36.6013071895425, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI18859983, Length=130, Percent_Identity=37.6923076923077, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24642371, Length=130, Percent_Identity=37.6923076923077, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24651058, Length=135, Percent_Identity=35.5555555555556, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 19281; Mature: 19281

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWHILKQIARTGIITEPAPGIDDAQRTAIERIHRDILLILGQALTIRAVDAGSCNGCELE
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEE
IHALNNPYYNIEGLGIKFVASPRHADMLLVTGPVSRHMDEALRRTYDATPEPKLVVAVGD
EEECCCCEEEEECCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECC
CGCTGGIFGDSYASCGRVANVIPVDVTVPGCPPPPIEILRGILTAVRQRRPGTAGDNPAG
CCCCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
S
C
>Mature Secondary Structure
MWHILKQIARTGIITEPAPGIDDAQRTAIERIHRDILLILGQALTIRAVDAGSCNGCELE
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEE
IHALNNPYYNIEGLGIKFVASPRHADMLLVTGPVSRHMDEALRRTYDATPEPKLVVAVGD
EEECCCCEEEEECCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECC
CGCTGGIFGDSYASCGRVANVIPVDVTVPGCPPPPIEILRGILTAVRQRRPGTAGDNPAG
CCCCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]