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The map label for this gene is hyfF [H]

Identifier: 91786821

GI number: 91786821

Start: 935817

End: 937280

Strand: Direct

Name: hyfF [H]

Synonym: Bpro_0919

Alternate gene names: 91786821

Gene position: 935817-937280 (Clockwise)

Preceding gene: 91786820

Following gene: 91786822

Centisome position: 18.0

GC content: 63.32

Gene sequence:

>1464_bases
ATGACTGAGTTGCTTGCCCTGGCCTTCCTCCTCGCAACCCCGCTCGCCGGCGGGCTGGTGCTGGCGCTGGTCGGCCACCG
CGACAACGCGCGCGATGTCAACGTCGCCTTCAGCCTGGCAACCTTCATTGCCGCTTGCGTGCTCACGATACAGATCATCG
AAGGCGGGCCGCTGCTGGTGTGGGGCAACGAGTTCTACATCGATGCGCTGAACGTCTTTCTCGTCTCGCTGACCGCGTTT
GTCGGCTTCACTACCTCGATCTTCTCGCGGCCCTATATGCGCATCGAGCGCGACCGCGGCAAGATGACCCCGCCGCGCCT
GCGCCTTTATCACAGCATGTACCAGCTGTTCAGCTTCACGATGCTGCTGGCACTGACGACCAACAACATGGGCATTCTCT
GGGTCGCGATGGAGGCCGCCACGCTGACCACCGTGCTGCTGGTCAGCGTCTACCGCACCGCGGCCAGCCTGGAGGCCGCG
TGGAAATACTTCATCCTGTGCGGTGTGGGTATTGCCCAGGCGCTGTTCGGCACGGTGCTGCTCTACATGGCGGCCGAGAA
GGTCATCAGCGCCGAGGCCGGTGCCTTGCTCTGGACCAACCTCGATGCGGTGAAGAATCAGCTCGACCCGAACATCATTA
CGCTGGCGTTCGCGTTCCTGTTCATCGGCTACGGCACCAAGGTTGGCCTGGTGCCGTTGCACAACTGGTTGCCGGACGCC
CATGCCGAGGGGCCTACGCCCGTGTCCGCCGTGTTGTCGGGCCTGCTGCTGAACGTGGCGCTCTATGCGATCCTGCGCTG
CAAGGTGCTGACGGACGGTGCGCTGCACAGTCATCTGGCCGGCCGGCTGATGATGGGCTTCGGCCTGTTGTCGGTGGTGG
CTGCGGTGTTCCTCATGATCCGGCAGAAGGACGTCAAGCGCATGTTCGCCTATTCGTCCATCGAGCACATGGGGATGATG
ACCTTCGCGTTCGGCATGGGCGGCCCGATCGCCAACTTCGCCGGGCTGCTGCACATGACGGTGCATTCGCTGATCAAGTC
CGCGATCTTTTTTGCCGTCGGCCACGCCACGCAGAAGGCCGGTACACAGGTCATGGAAAACATTCGCGGGCTGATCAAGG
TCAGCCCGACGGTCGCCTGGGGCCTGATGCTCGGATCTCTGGCCATCCTGGGTATGCCGCCATTCGGGGTTTTCGCCAGC
GAGTTCCTGATCGTCACGACCGCGATGCGCGAGCAGCCCTGGGCGACGCCGTTCCTGTTGATCGCCTTGGGCCTGGCATT
TGCATCGGTCTTCAGCCGCATCCAGCCCATGGTGTTCGGCGAGACCAACGTCCAGCCCCTGGCGCATCCGCCAGCACTGA
TCCCGGTGTTTGTTCACCTGGCGCTCGGGCTGATGCTGGGCCTGTACATTCCGTCCTATCTCAACGCCTGGTACCGGCAG
GCGGCCGCCATGCTGGGAAGCTGA

Upstream 100 bases:

>100_bases
CTGGATGTGCTGATCGGCGTGCTGATCCTGGGCGTGTTCATGTTCCAGATCCGCGAGCAGTTCGACAGCCTGGACATCAG
CCATCTCGAAAAACTCAAGG

Downstream 100 bases:

>100_bases
CCGTATGGCGCTGCCTGACCTTGGACTCGACCTGCGGCGCCTGCCGGCGCCCCTGCCTATGTGGCACGGACAGGTCGGTC
GCGACGCCTGGAGCTCCGCG

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MTELLALAFLLATPLAGGLVLALVGHRDNARDVNVAFSLATFIAACVLTIQIIEGGPLLVWGNEFYIDALNVFLVSLTAF
VGFTTSIFSRPYMRIERDRGKMTPPRLRLYHSMYQLFSFTMLLALTTNNMGILWVAMEAATLTTVLLVSVYRTAASLEAA
WKYFILCGVGIAQALFGTVLLYMAAEKVISAEAGALLWTNLDAVKNQLDPNIITLAFAFLFIGYGTKVGLVPLHNWLPDA
HAEGPTPVSAVLSGLLLNVALYAILRCKVLTDGALHSHLAGRLMMGFGLLSVVAAVFLMIRQKDVKRMFAYSSIEHMGMM
TFAFGMGGPIANFAGLLHMTVHSLIKSAIFFAVGHATQKAGTQVMENIRGLIKVSPTVAWGLMLGSLAILGMPPFGVFAS
EFLIVTTAMREQPWATPFLLIALGLAFASVFSRIQPMVFGETNVQPLAHPPALIPVFVHLALGLMLGLYIPSYLNAWYRQ
AAAMLGS

Sequences:

>Translated_487_residues
MTELLALAFLLATPLAGGLVLALVGHRDNARDVNVAFSLATFIAACVLTIQIIEGGPLLVWGNEFYIDALNVFLVSLTAF
VGFTTSIFSRPYMRIERDRGKMTPPRLRLYHSMYQLFSFTMLLALTTNNMGILWVAMEAATLTTVLLVSVYRTAASLEAA
WKYFILCGVGIAQALFGTVLLYMAAEKVISAEAGALLWTNLDAVKNQLDPNIITLAFAFLFIGYGTKVGLVPLHNWLPDA
HAEGPTPVSAVLSGLLLNVALYAILRCKVLTDGALHSHLAGRLMMGFGLLSVVAAVFLMIRQKDVKRMFAYSSIEHMGMM
TFAFGMGGPIANFAGLLHMTVHSLIKSAIFFAVGHATQKAGTQVMENIRGLIKVSPTVAWGLMLGSLAILGMPPFGVFAS
EFLIVTTAMREQPWATPFLLIALGLAFASVFSRIQPMVFGETNVQPLAHPPALIPVFVHLALGLMLGLYIPSYLNAWYRQ
AAAMLGS
>Mature_486_residues
TELLALAFLLATPLAGGLVLALVGHRDNARDVNVAFSLATFIAACVLTIQIIEGGPLLVWGNEFYIDALNVFLVSLTAFV
GFTTSIFSRPYMRIERDRGKMTPPRLRLYHSMYQLFSFTMLLALTTNNMGILWVAMEAATLTTVLLVSVYRTAASLEAAW
KYFILCGVGIAQALFGTVLLYMAAEKVISAEAGALLWTNLDAVKNQLDPNIITLAFAFLFIGYGTKVGLVPLHNWLPDAH
AEGPTPVSAVLSGLLLNVALYAILRCKVLTDGALHSHLAGRLMMGFGLLSVVAAVFLMIRQKDVKRMFAYSSIEHMGMMT
FAFGMGGPIANFAGLLHMTVHSLIKSAIFFAVGHATQKAGTQVMENIRGLIKVSPTVAWGLMLGSLAILGMPPFGVFASE
FLIVTTAMREQPWATPFLLIALGLAFASVFSRIQPMVFGETNVQPLAHPPALIPVFVHLALGLMLGLYIPSYLNAWYRQA
AAMLGS

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=380, Percent_Identity=26.5789473684211, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI251831116, Length=305, Percent_Identity=24.9180327868852, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788831, Length=359, Percent_Identity=43.1754874651811, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1788827, Length=350, Percent_Identity=29.1428571428571, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788613, Length=231, Percent_Identity=31.6017316017316, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788829, Length=420, Percent_Identity=25.7142857142857, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788614, Length=358, Percent_Identity=26.8156424581006, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI145693160, Length=268, Percent_Identity=25.3731343283582, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI2367154, Length=194, Percent_Identity=26.8041237113402, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 52739; Mature: 52607

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELLALAFLLATPLAGGLVLALVGHRDNARDVNVAFSLATFIAACVLTIQIIEGGPLLV
CHHHHHHHHHHHCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEE
WGNEFYIDALNVFLVSLTAFVGFTTSIFSRPYMRIERDRGKMTPPRLRLYHSMYQLFSFT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHH
MLLALTTNNMGILWVAMEAATLTTVLLVSVYRTAASLEAAWKYFILCGVGIAQALFGTVL
HHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYMAAEKVISAEAGALLWTNLDAVKNQLDPNIITLAFAFLFIGYGTKVGLVPLHNWLPDA
HHHHHHHHHHHCCCEEEEECHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEECHHCCCCC
HAEGPTPVSAVLSGLLLNVALYAILRCKVLTDGALHSHLAGRLMMGFGLLSVVAAVFLMI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQKDVKRMFAYSSIEHMGMMTFAFGMGGPIANFAGLLHMTVHSLIKSAIFFAVGHATQKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTQVMENIRGLIKVSPTVAWGLMLGSLAILGMPPFGVFASEFLIVTTAMREQPWATPFLL
HHHHHHHHHHHEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
IALGLAFASVFSRIQPMVFGETNVQPLAHPPALIPVFVHLALGLMLGLYIPSYLNAWYRQ
HHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAMLGS
HHHHHCC
>Mature Secondary Structure 
TELLALAFLLATPLAGGLVLALVGHRDNARDVNVAFSLATFIAACVLTIQIIEGGPLLV
HHHHHHHHHHHCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEE
WGNEFYIDALNVFLVSLTAFVGFTTSIFSRPYMRIERDRGKMTPPRLRLYHSMYQLFSFT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHH
MLLALTTNNMGILWVAMEAATLTTVLLVSVYRTAASLEAAWKYFILCGVGIAQALFGTVL
HHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYMAAEKVISAEAGALLWTNLDAVKNQLDPNIITLAFAFLFIGYGTKVGLVPLHNWLPDA
HHHHHHHHHHHCCCEEEEECHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEECHHCCCCC
HAEGPTPVSAVLSGLLLNVALYAILRCKVLTDGALHSHLAGRLMMGFGLLSVVAAVFLMI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQKDVKRMFAYSSIEHMGMMTFAFGMGGPIANFAGLLHMTVHSLIKSAIFFAVGHATQKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTQVMENIRGLIKVSPTVAWGLMLGSLAILGMPPFGVFASEFLIVTTAMREQPWATPFLL
HHHHHHHHHHHEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
IALGLAFASVFSRIQPMVFGETNVQPLAHPPALIPVFVHLALGLMLGLYIPSYLNAWYRQ
HHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAMLGS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA