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The map label for this gene is otsA [H]

Identifier: 89054554

GI number: 89054554

Start: 2071478

End: 2072836

Strand: Direct

Name: otsA [H]

Synonym: Jann_2063

Alternate gene names: 89054554

Gene position: 2071478-2072836 (Clockwise)

Preceding gene: 89054553

Following gene: 89054555

Centisome position: 47.97

GC content: 61.52

Gene sequence:

>1359_bases
ATGTCTCGTTTGGTCGTCGTCTCGAACCGGCTGCCCAAAGACCAGACCCCTGCGGGCGGGCTCGTCTTCGCGCTGCACGA
ACTCTTGTCCCGGCAAGGGGGCATCTGGATCGGGGCCGATGATCCGGGGGAGACGGGCGACGGCCCGCTGAAACAGATCG
GATCGGGTGTTTACGACCGCTTTGTGTTTCCGCTGAGCCAGTCGGAGAATGACAAATATTATCTTGGCTACGCGAACTCA
GCGCTCTGGCCCACTTTCCATTCCCGCCGTGATCTGATGCGCCATCGCGGCGAGTGGTTTGCGGCCTATTATGACGTCAA
CCAGCGGCTCGCACGCCTATTGGCCGAGATGTTGCAGCCCGACGACCTGGTCTGGATCCATGATTACCATTTCCTGCCGC
TCGCCTCAAAACTGCGGGAGCTGAAAGTCACCAACAAGATCGGCTTCTTCCTTCATATTCCATTCCCCTCCACCACCGAC
ATCACCGCCTTGCCCGAGCATACGATGATGCCTGACTGGCTGTCGGCCTACGACCTGCTGGGGCTGCAAACCCAACGGGA
TGTGTCGGCCTGTCGCGAGTATCTGCGCCAATCCGGTGAGGTGGAGCTTTTGATGTCGGGCGGGCTCTTGCAGCGAAATC
ACCGCACGTTCACCGTCAACAGCTTCCCGATCGGCGTGGATGCGGCGGCCATCGCGGCAGATGCAAAGGCCGCCCATGCC
GATGTGGCGGCCCGGGTCAAAACGGCGGGGCCGCTGATCTTGGGGGTCGACCGGCTTGATTATTCCAAGGGTCTGATCCA
GCGGGTTGAAGGGTTCGGGCGGTACCTGGACCTCTATAATTCCGGCGACCTGCGCCCCACATATCTGCAAATCGCGCCGG
TCAGCCGGGGTGATGTGACGGCCTACAGGACGCTCCGGCAGCAACTGGACGAGATTGCGGGATCGGTCAACGGCACCCAC
GCCGATCTGGACTGGACGCCCATCCGATACGTGCGGCGCAATGTGTCGCGTGACGTGATCTCGGCCCTCTACCGTCATGC
GGACATTTGCCTGGTGACGCCGCTGGCGGACGGCATGAACCTTGTGGCGAAGGAGTTTATTGCAGCCCAGGACCCCGAGG
ATCCCGGCGTGCTGATCCTGTCGCAATTTGCTGGCGCGGCCGAACAGTTGCAGGATGCGCTCTCCATCAATCCCTTCGAC
ACCGACAGTATCGCGGCGACCATTTCGCGGGCCATCTCAATGCCTCTGGAGGAGCGTCAGGCGCGTCATGCGACCCAGGC
CGACAGCGTGTTCTCCCAGGATATCGCCTGGTGGGGCAGGACCTATCTTGATCGGCTGACCAATCTCGCGACGGACTAG

Upstream 100 bases:

>100_bases
GTGGCCAAGCGATCAGCGTCGGTGGTATCCATGCCGCAGCCGATATTCACCTGGCCGCACCCCAGGACGTGCGCCGCCTT
CTCAAAGATTGGATCGTCTG

Downstream 100 bases:

>100_bases
TCGGCGGCGTTCGCAAGGGACCATCAGGGTATTCTTGCGCGTCGAAGGGAACCTGCTTGAGGTCCGGGAAGTTGGTCCCG
GGAAGCGCCAGATGCGCGCC

Product: Alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSRLVVVSNRLPKDQTPAGGLVFALHELLSRQGGIWIGADDPGETGDGPLKQIGSGVYDRFVFPLSQSENDKYYLGYANS
ALWPTFHSRRDLMRHRGEWFAAYYDVNQRLARLLAEMLQPDDLVWIHDYHFLPLASKLRELKVTNKIGFFLHIPFPSTTD
ITALPEHTMMPDWLSAYDLLGLQTQRDVSACREYLRQSGEVELLMSGGLLQRNHRTFTVNSFPIGVDAAAIAADAKAAHA
DVAARVKTAGPLILGVDRLDYSKGLIQRVEGFGRYLDLYNSGDLRPTYLQIAPVSRGDVTAYRTLRQQLDEIAGSVNGTH
ADLDWTPIRYVRRNVSRDVISALYRHADICLVTPLADGMNLVAKEFIAAQDPEDPGVLILSQFAGAAEQLQDALSINPFD
TDSIAATISRAISMPLEERQARHATQADSVFSQDIAWWGRTYLDRLTNLATD

Sequences:

>Translated_452_residues
MSRLVVVSNRLPKDQTPAGGLVFALHELLSRQGGIWIGADDPGETGDGPLKQIGSGVYDRFVFPLSQSENDKYYLGYANS
ALWPTFHSRRDLMRHRGEWFAAYYDVNQRLARLLAEMLQPDDLVWIHDYHFLPLASKLRELKVTNKIGFFLHIPFPSTTD
ITALPEHTMMPDWLSAYDLLGLQTQRDVSACREYLRQSGEVELLMSGGLLQRNHRTFTVNSFPIGVDAAAIAADAKAAHA
DVAARVKTAGPLILGVDRLDYSKGLIQRVEGFGRYLDLYNSGDLRPTYLQIAPVSRGDVTAYRTLRQQLDEIAGSVNGTH
ADLDWTPIRYVRRNVSRDVISALYRHADICLVTPLADGMNLVAKEFIAAQDPEDPGVLILSQFAGAAEQLQDALSINPFD
TDSIAATISRAISMPLEERQARHATQADSVFSQDIAWWGRTYLDRLTNLATD
>Mature_451_residues
SRLVVVSNRLPKDQTPAGGLVFALHELLSRQGGIWIGADDPGETGDGPLKQIGSGVYDRFVFPLSQSENDKYYLGYANSA
LWPTFHSRRDLMRHRGEWFAAYYDVNQRLARLLAEMLQPDDLVWIHDYHFLPLASKLRELKVTNKIGFFLHIPFPSTTDI
TALPEHTMMPDWLSAYDLLGLQTQRDVSACREYLRQSGEVELLMSGGLLQRNHRTFTVNSFPIGVDAAAIAADAKAAHAD
VAARVKTAGPLILGVDRLDYSKGLIQRVEGFGRYLDLYNSGDLRPTYLQIAPVSRGDVTAYRTLRQQLDEIAGSVNGTHA
DLDWTPIRYVRRNVSRDVISALYRHADICLVTPLADGMNLVAKEFIAAQDPEDPGVLILSQFAGAAEQLQDALSINPFDT
DSIAATISRAISMPLEERQARHATQADSVFSQDIAWWGRTYLDRLTNLATD

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=462, Percent_Identity=42.8571428571429, Blast_Score=347, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI115533560, Length=358, Percent_Identity=27.9329608938547, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115533558, Length=358, Percent_Identity=27.9329608938547, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71987012, Length=445, Percent_Identity=25.8426966292135, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319602, Length=472, Percent_Identity=31.1440677966102, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6320279, Length=381, Percent_Identity=28.3464566929134, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6323537, Length=433, Percent_Identity=26.5588914549654, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323917, Length=392, Percent_Identity=25.2551020408163, Blast_Score=123, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19920676, Length=496, Percent_Identity=34.6774193548387, Blast_Score=256, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 50374; Mature: 50242

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVVVSNRLPKDQTPAGGLVFALHELLSRQGGIWIGADDPGETGDGPLKQIGSGVYDR
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCHHHHH
FVFPLSQSENDKYYLGYANSALWPTFHSRRDLMRHRGEWFAAYYDVNQRLARLLAEMLQP
EEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCC
DDLVWIHDYHFLPLASKLRELKVTNKIGFFLHIPFPSTTDITALPEHTMMPDWLSAYDLL
CCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCCCCCHHHHHHHHH
GLQTQRDVSACREYLRQSGEVELLMSGGLLQRNHRTFTVNSFPIGVDAAAIAADAKAAHA
CCCCHHHHHHHHHHHHCCCCEEEEECCCEEECCCCEEEEECCCCCCCHHHHHHCCHHHHH
DVAARVKTAGPLILGVDRLDYSKGLIQRVEGFGRYLDLYNSGDLRPTYLQIAPVSRGDVT
HHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHH
AYRTLRQQLDEIAGSVNGTHADLDWTPIRYVRRNVSRDVISALYRHADICLVTPLADGMN
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCHH
LVAKEFIAAQDPEDPGVLILSQFAGAAEQLQDALSINPFDTDSIAATISRAISMPLEERQ
HHHHHHHHCCCCCCCCEEEEHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHH
ARHATQADSVFSQDIAWWGRTYLDRLTNLATD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRLVVVSNRLPKDQTPAGGLVFALHELLSRQGGIWIGADDPGETGDGPLKQIGSGVYDR
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCHHHHH
FVFPLSQSENDKYYLGYANSALWPTFHSRRDLMRHRGEWFAAYYDVNQRLARLLAEMLQP
EEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCC
DDLVWIHDYHFLPLASKLRELKVTNKIGFFLHIPFPSTTDITALPEHTMMPDWLSAYDLL
CCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCCCCCHHHHHHHHH
GLQTQRDVSACREYLRQSGEVELLMSGGLLQRNHRTFTVNSFPIGVDAAAIAADAKAAHA
CCCCHHHHHHHHHHHHCCCCEEEEECCCEEECCCCEEEEECCCCCCCHHHHHHCCHHHHH
DVAARVKTAGPLILGVDRLDYSKGLIQRVEGFGRYLDLYNSGDLRPTYLQIAPVSRGDVT
HHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHH
AYRTLRQQLDEIAGSVNGTHADLDWTPIRYVRRNVSRDVISALYRHADICLVTPLADGMN
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCHH
LVAKEFIAAQDPEDPGVLILSQFAGAAEQLQDALSINPFDTDSIAATISRAISMPLEERQ
HHHHHHHHCCCCCCCCEEEEHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHH
ARHATQADSVFSQDIAWWGRTYLDRLTNLATD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA