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The map label for this gene is otsB [H]

Identifier: 89054553

GI number: 89054553

Start: 2070741

End: 2071478

Strand: Direct

Name: otsB [H]

Synonym: Jann_2062

Alternate gene names: 89054553

Gene position: 2070741-2071478 (Clockwise)

Preceding gene: 89054552

Following gene: 89054554

Centisome position: 47.96

GC content: 64.77

Gene sequence:

>738_bases
ATGCCTGATACCCCATCGTCAAACCTGCCTGCGCCGGCGCGGGCGTCCCTGTTTCTTGATTTCGACGGAACGCTCGTTGA
CATCGCGCCGACGCCCGATGGCATTACCGTGCCGGATGATCTGGCGGACCTGTTGGCCATGCTGGACACGGCCCTCGGCG
GGCGTCTGGCGCTGGTGTCGGGGCGGGCCATCGCCAGTCTTGAGCAGCACCTGCCGACGTTTCAGGGGATCATCGTCGGC
GGTCATGGCGCAGAGATGCGGGGGCGCGATGGGCGCATAGAGCGTCTTGCAGGGGCCGATAGCGGTATCGCGGATCTGCA
TTTCTGGGCGACCGGGATTGAAGGCGATTTGGCGGGTGTCCGGGCGGAGTTGAAACGCACCGGCGCGGCGCTGCATTTCC
GTCAGGCGCCGCAGCACGAGGATGCGTTGCACGCAGCCGCGCAGACGTATCTGGCGACGCACCCGGATTTGGCGTTGCAG
ATGGCGCACATGACCGTCGAGCTACGGCCAAAGGATGCCAACAAGGCCAGCGCGGTGGCGACGCTGATGCACTCGCCCCC
CTTTGTGGGCCATCCTCCGATCTTCGCAGGCGATGACGCGACGGATATTCCGGCCATGGACCATTGCCAAACCGCAGGTG
GCCAAGCGATCAGCGTCGGTGGTATCCATGCCGCAGCCGATATTCACCTGGCCGCACCCCAGGACGTGCGCCGCCTTCTC
AAAGATTGGATCGTCTGA

Upstream 100 bases:

>100_bases
CACGTCGATATGAGGTCGTCCGAGCGAGGGATCGGATGGATGCTGCTTGGATGCGACAGAAATGCGGCGGCCTGGACCGC
CGGGACAAGAGGGTTCGACC

Downstream 100 bases:

>100_bases
TGTCTCGTTTGGTCGTCGTCTCGAACCGGCTGCCCAAAGACCAGACCCCTGCGGGCGGGCTCGTCTTCGCGCTGCACGAA
CTCTTGTCCCGGCAAGGGGG

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPDTPSSNLPAPARASLFLDFDGTLVDIAPTPDGITVPDDLADLLAMLDTALGGRLALVSGRAIASLEQHLPTFQGIIVG
GHGAEMRGRDGRIERLAGADSGIADLHFWATGIEGDLAGVRAELKRTGAALHFRQAPQHEDALHAAAQTYLATHPDLALQ
MAHMTVELRPKDANKASAVATLMHSPPFVGHPPIFAGDDATDIPAMDHCQTAGGQAISVGGIHAAADIHLAAPQDVRRLL
KDWIV

Sequences:

>Translated_245_residues
MPDTPSSNLPAPARASLFLDFDGTLVDIAPTPDGITVPDDLADLLAMLDTALGGRLALVSGRAIASLEQHLPTFQGIIVG
GHGAEMRGRDGRIERLAGADSGIADLHFWATGIEGDLAGVRAELKRTGAALHFRQAPQHEDALHAAAQTYLATHPDLALQ
MAHMTVELRPKDANKASAVATLMHSPPFVGHPPIFAGDDATDIPAMDHCQTAGGQAISVGGIHAAADIHLAAPQDVRRLL
KDWIV
>Mature_244_residues
PDTPSSNLPAPARASLFLDFDGTLVDIAPTPDGITVPDDLADLLAMLDTALGGRLALVSGRAIASLEQHLPTFQGIIVGG
HGAEMRGRDGRIERLAGADSGIADLHFWATGIEGDLAGVRAELKRTGAALHFRQAPQHEDALHAAAQTYLATHPDLALQM
AHMTVELRPKDANKASAVATLMHSPPFVGHPPIFAGDDATDIPAMDHCQTAGGQAISVGGIHAAADIHLAAPQDVRRLLK
DWIV

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=229, Percent_Identity=36.2445414847162, Blast_Score=144, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 25670; Mature: 25539

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTPSSNLPAPARASLFLDFDGTLVDIAPTPDGITVPDDLADLLAMLDTALGGRLALVS
CCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEC
GRAIASLEQHLPTFQGIIVGGHGAEMRGRDGRIERLAGADSGIADLHFWATGIEGDLAGV
CHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHH
RAELKRTGAALHFRQAPQHEDALHAAAQTYLATHPDLALQMAHMTVELRPKDANKASAVA
HHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHEEEEEEEEEECCCCCCHHHHHH
TLMHSPPFVGHPPIFAGDDATDIPAMDHCQTAGGQAISVGGIHAAADIHLAAPQDVRRLL
HHHCCCCCCCCCCEECCCCCCCCCCHHHHHCCCCCEEEECCEEEEEEEEEECCHHHHHHH
KDWIV
HHHCC
>Mature Secondary Structure 
PDTPSSNLPAPARASLFLDFDGTLVDIAPTPDGITVPDDLADLLAMLDTALGGRLALVS
CCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEC
GRAIASLEQHLPTFQGIIVGGHGAEMRGRDGRIERLAGADSGIADLHFWATGIEGDLAGV
CHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHH
RAELKRTGAALHFRQAPQHEDALHAAAQTYLATHPDLALQMAHMTVELRPKDANKASAVA
HHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHEEEEEEEEEECCCCCCHHHHHH
TLMHSPPFVGHPPIFAGDDATDIPAMDHCQTAGGQAISVGGIHAAADIHLAAPQDVRRLL
HHHCCCCCCCCCCEECCCCCCCCCCHHHHHCCCCCEEEECCEEEEEEEEEECCHHHHHHH
KDWIV
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503 [H]