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The map label for this gene is alaXM [H]

Identifier: 89054486

GI number: 89054486

Start: 1995838

End: 1996578

Strand: Direct

Name: alaXM [H]

Synonym: Jann_1995

Alternate gene names: 89054486

Gene position: 1995838-1996578 (Clockwise)

Preceding gene: 89054485

Following gene: 89054487

Centisome position: 46.22

GC content: 64.24

Gene sequence:

>741_bases
ATGACGACCGAGACCTTGTTCCTCAAGGATCCGTATCGCACCTCCGCCACAGCCCGCGTCATTGGCCAGACGGAGGAGGG
CGGCGTGATCCTTGATCGCACCATCTTCTATGCGCGCGGCGGGGGGCAACCCGGTGACAGTGGGGCACTGACCTGGGACG
GCCATCGCCTGCCTATCGCCACGGCAGTGAAAGGGCCGATCCCCGTTGAAGGGGCGGGTGGTGCGATTGTTCTGATCCCC
GCAGAGCCCGCGCCGCTGCCGCCTGTGGGCGCAGAGGTTGAACAGCAACTCGACTGGGACCGCCGGCTTGGCCACATGCG
CATCCACACGGCGTTGCATCTGTTGTCCGTCGTGATCCCTCTGCCCGTGACCGGCGGGGCCGTGGCCGCCGACAAGGGAC
GGCTTGATTTCCTGATGCCGCAACCGCCCGAAGACAAGATGATCCTGGAAGGGCAGTTGAATGCGCTGATCACCCGCGAT
CTGCCCGTCAGCGTGGAGTGGATCACCGAGAGTCAGTTGAACGCAAACCCAAACCTCGTGAAGACACTGTCTGTCCAGCC
GCCAAGCGGGGCGGGCGAGATCCGTCTGGTGCGTATCGGCGATGAGGCCGCGCCGGTTGATTTGCAGCCCTGCGGCGGGA
CCCACGTGGCACGGACCTCCGAGATCGGGAAACTTGCCATCACCAAGGTGGAGAACAAGGGCAAGCAGAACCGTCGCGTC
ACAATCGAGTTCGCGTCGTGA

Upstream 100 bases:

>100_bases
TCTGGTTGCGCAATCCCGAGACGCTGCAACCACTGCAATCCAAGCCGGAACCACAGCCCCAAGCCGGGCCTTCCACGCCG
CCACAGGAGGCCTAAGCCCC

Downstream 100 bases:

>100_bases
GCCTTTATGGTGCACTTTACACCGCCGCGGGCGTGTTCGCGCTGCTGTTGGGGGTGGTGCTTATTCTGATCCCGCAGCTT
TACCTGTCGCTATACGTGGC

Product: metal-dependent hydrolases related to alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTTETLFLKDPYRTSATARVIGQTEEGGVILDRTIFYARGGGQPGDSGALTWDGHRLPIATAVKGPIPVEGAGGAIVLIP
AEPAPLPPVGAEVEQQLDWDRRLGHMRIHTALHLLSVVIPLPVTGGAVAADKGRLDFLMPQPPEDKMILEGQLNALITRD
LPVSVEWITESQLNANPNLVKTLSVQPPSGAGEIRLVRIGDEAAPVDLQPCGGTHVARTSEIGKLAITKVENKGKQNRRV
TIEFAS

Sequences:

>Translated_246_residues
MTTETLFLKDPYRTSATARVIGQTEEGGVILDRTIFYARGGGQPGDSGALTWDGHRLPIATAVKGPIPVEGAGGAIVLIP
AEPAPLPPVGAEVEQQLDWDRRLGHMRIHTALHLLSVVIPLPVTGGAVAADKGRLDFLMPQPPEDKMILEGQLNALITRD
LPVSVEWITESQLNANPNLVKTLSVQPPSGAGEIRLVRIGDEAAPVDLQPCGGTHVARTSEIGKLAITKVENKGKQNRRV
TIEFAS
>Mature_245_residues
TTETLFLKDPYRTSATARVIGQTEEGGVILDRTIFYARGGGQPGDSGALTWDGHRLPIATAVKGPIPVEGAGGAIVLIPA
EPAPLPPVGAEVEQQLDWDRRLGHMRIHTALHLLSVVIPLPVTGGAVAADKGRLDFLMPQPPEDKMILEGQLNALITRDL
PVSVEWITESQLNANPNLVKTLSVQPPSGAGEIRLVRIGDEAAPVDLQPCGGTHVARTSEIGKLAITKVENKGKQNRRVT
IEFAS

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) or Gly-tRNA(Ala). Has no activity on incorrectly charged Ser-tRNA(Thr), nor on correctly charged Ala-tRNA(Ala) or Ser-tRNA(Ser) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789048, Length=229, Percent_Identity=27.9475982532751, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17506981, Length=233, Percent_Identity=27.0386266094421, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17536681, Length=247, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 26211; Mature: 26080

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTETLFLKDPYRTSATARVIGQTEEGGVILDRTIFYARGGGQPGDSGALTWDGHRLPIA
CCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCEEECCCCCEEE
TAVKGPIPVEGAGGAIVLIPAEPAPLPPVGAEVEQQLDWDRRLGHMRIHTALHLLSVVIP
EECCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHC
LPVTGGAVAADKGRLDFLMPQPPEDKMILEGQLNALITRDLPVSVEWITESQLNANPNLV
CCCCCCEEEECCCCEEEECCCCCCCCEEEECCCEEEEEECCCEEEEEEECCCCCCCCCEE
KTLSVQPPSGAGEIRLVRIGDEAAPVDLQPCGGTHVARTSEIGKLAITKVENKGKQNRRV
EEEEECCCCCCCCEEEEEECCCCCCEEECCCCCCCEECCCCCCEEEEEEECCCCCCCCEE
TIEFAS
EEEECC
>Mature Secondary Structure 
TTETLFLKDPYRTSATARVIGQTEEGGVILDRTIFYARGGGQPGDSGALTWDGHRLPIA
CCCEEEEECCCCCCCEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCEEECCCCCEEE
TAVKGPIPVEGAGGAIVLIPAEPAPLPPVGAEVEQQLDWDRRLGHMRIHTALHLLSVVIP
EECCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHC
LPVTGGAVAADKGRLDFLMPQPPEDKMILEGQLNALITRDLPVSVEWITESQLNANPNLV
CCCCCCEEEECCCCEEEECCCCCCCCEEEECCCEEEEEECCCEEEEEEECCCCCCCCCEE
KTLSVQPPSGAGEIRLVRIGDEAAPVDLQPCGGTHVARTSEIGKLAITKVENKGKQNRRV
EEEEECCCCCCCCEEEEEECCCCCCEEECCCCCCCEECCCCCCEEEEEEECCCCCCCCEE
TIEFAS
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA