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The map label for this gene is kefA [C]

Identifier: 89054485

GI number: 89054485

Start: 1993442

End: 1995832

Strand: Direct

Name: kefA [C]

Synonym: Jann_1994

Alternate gene names: 89054485

Gene position: 1993442-1995832 (Clockwise)

Preceding gene: 89054484

Following gene: 89054486

Centisome position: 46.17

GC content: 61.44

Gene sequence:

>2391_bases
ATGACGGCTATTGTCGGACGATTGCTGACAGCGCTATTTTTGGCGCTGATACCGGTTATGGCCGCTATGCCGGGTGCGGT
TGTCGCGCAGACGACGGCCCCCGACGCAGAGATCGACTACGAGGCCTGGGAACGTGAGGCCGCCGCCACGGAAGCGGTGA
TCGAGGGCGCACGGGCGTCGACGTCCTTGCTGGAAACACGCCGTTTGGAACTGGTGCGTTGGCGGGCGCAGTTTCTCGCA
TCGGATGCCAGCAACGAAGAGCGGATCGCAACGATCCAAAGCCAGATTGACGCGCTGGGGCCCGGCCCGGCGGACGGAGA
CGTCGAACCTGACGCAATTGCCGACCGACGGACGGCCCTGGGAGAGGCGATGACGGCCGCACAAGCGCCGGGCTTGCAGG
CCAGAGAGGCGTTCAATCGTGCCAATGGCCTGATCGCCGAGATCGACACGATACTGCTGGAACGTCAGACGCAACTGTTG
TTGGAACGCGACCCTACGCCGCTGAACCCGGCAAATTGGGCCATTGCGATCAACGCGCTGGTAGAGTTTGTGCGCGATCA
GCAGCGGCAGAAAGGCGGACTTCCCCAGGACCCGGACAGTCTTTTGTCGGCTGCCGAAACCTTGCCGCTGCTCATTTTCG
CGGTCGTGGTTGGACTGGCACTTATCGTCTATGGCCGACCATTTGTGGCGCGATTGGGTGCCCGTTTTTTGGATACGGAC
CGCCGAAGGGGCCGGACGGCTCTTGGGTTCGCGGTGTCATTGGGGCAGCTGCTGCTGCCGTTGGTTGGCTTTCTGGTTAT
TATCCTGGCGCTGGATCTATCCGGCCTTCTCACCGAAGATCTTGACGCGCTGGCGGTCGCCGTCGGCGCATTAATCCTGT
CCGTCTACATCGCCCTCTGGATCGCTGGTCGGCTTTTTCCAGATCACGAGGAACGCGCCGCAGCCTTTGACATTCAAATG
CCCGCTCGGTCCCAGGCGCGGCGCACCATCTTGTTTATCGGCGTCTTTGCGGGCCTTGCGGCTTTTGCGGAGGCGGTGGC
CGCATTCGATGTCATTCCACCCGCCGCGAGAGGCGTGTTTATCCTGCCCGCCTATGCCGGTCTTGCGTGGATGTTCATAC
GCTTCGGACGGCTGATGCGGGCCGCGCGCGCTGAGGTTGAGGATGGGGACTCGTCCAATTTCGGCCAGCGGGTCGTGGGC
CTCCTGACGCGGGCGCTTACGGTCATCGCCGTCGTCGGCCCGGTGTTGGTCGTGTTCGGCTATATCAACGCCGCCGAAGC
GATCATGTTGCCAACGGTGACGACGCTTCTGATCCTGGGTATTCTGCTCGTGTTGCAATCCGTGATCCGTGATCTTTACG
CGGTCCTCTTTCGCTCAACGGTTGAAGCGGCGGCAGATGCATTGCTTCCGGTCATCGTAAATTTCTCGCTGTTTGTTTTG
GCCACGCCGCTTCTTGCGCTTGTATGGGGTGTCCGCCCGGAACGGTTGCAGGAAATATACGCCCGTGTGCTGGAGGGGTT
CACCCTTGGCGATACGCGGATCACGCCGGGCATTGTGCTTGCGGTTCTTCTGGTCTTTGCCGCAGGGGTCCTACTGACGC
GCATCTTGCAGGGTGCCCTGAAATCCACCGTCTTGCCGCGCACGAAGCTGGATGTGGGCGCGCGCAATGCCGTCTCAGCA
GGCGTTGGCTATGTCGGGATCGCGTTGGCCTGCGTGATTGCCGTGACCAGCGCGGGCATCGACCTGACGGCACTTGGTTT
CGTCGTCGGCGCTCTGTCCGTCGGCATCGGTTTTGGCCTCCAGAACGTCGTCTCCAACTTTGTGTCCGGCATTATCCTCT
TGATTGAGCGCCCGATCAGCGAAGGGGACTGGATCGAGGTCGCGGGCAATATGGGGATCGTCAAGGATATCTCCGTGCGC
TCCACCACGATCGAGACCTTCGACAAGACCGACGTGATCGTGCCCAACGCGGATTTCATCAGCGGGACCGTCACCAACTG
GACGCGGGGCAATACCGTGGGCCGGGTGGTGCTGACCGTGGGCGTGGCCTATGGCAACGACACGCGCCGGGTGGCTGATC
TGCTGATGGAGATCGCCAATGGTCACGACGATGTCGTGAGCTTCCCCGCCCCGGGCGTTGATTTTCTGGGGTTCGGGGCC
GACAGCCTCGACTTCCGGGTCCGGGTAATCCTGCGCGACATCAACCTGTTCGTTACTGTCCAGACCGAGCTTTATCACCG
GATAAATGAGACATTTGTGCGCGAAGGTATCGAAATTCCCTTTGCACAGCGCGATGTCTGGTTGCGCAATCCCGAGACGC
TGCAACCACTGCAATCCAAGCCGGAACCACAGCCCCAAGCCGGGCCTTCCACGCCGCCACAGGAGGCCTAA

Upstream 100 bases:

>100_bases
AATCCAAGCTGTTTAACCCCGACTTCCTGCGCGAAAAGGGCCTGCCCGTGCCGCAATGGATGGACGCAAAGGGGCGCGAC
GTCCCGCAGGTCTTCGCGGA

Downstream 100 bases:

>100_bases
GCCCCATGACGACCGAGACCTTGTTCCTCAAGGATCCGTATCGCACCTCCGCCACAGCCCGCGTCATTGGCCAGACGGAG
GAGGGCGGCGTGATCCTTGA

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 796; Mature: 795

Protein sequence:

>796_residues
MTAIVGRLLTALFLALIPVMAAMPGAVVAQTTAPDAEIDYEAWEREAAATEAVIEGARASTSLLETRRLELVRWRAQFLA
SDASNEERIATIQSQIDALGPGPADGDVEPDAIADRRTALGEAMTAAQAPGLQAREAFNRANGLIAEIDTILLERQTQLL
LERDPTPLNPANWAIAINALVEFVRDQQRQKGGLPQDPDSLLSAAETLPLLIFAVVVGLALIVYGRPFVARLGARFLDTD
RRRGRTALGFAVSLGQLLLPLVGFLVIILALDLSGLLTEDLDALAVAVGALILSVYIALWIAGRLFPDHEERAAAFDIQM
PARSQARRTILFIGVFAGLAAFAEAVAAFDVIPPAARGVFILPAYAGLAWMFIRFGRLMRAARAEVEDGDSSNFGQRVVG
LLTRALTVIAVVGPVLVVFGYINAAEAIMLPTVTTLLILGILLVLQSVIRDLYAVLFRSTVEAAADALLPVIVNFSLFVL
ATPLLALVWGVRPERLQEIYARVLEGFTLGDTRITPGIVLAVLLVFAAGVLLTRILQGALKSTVLPRTKLDVGARNAVSA
GVGYVGIALACVIAVTSAGIDLTALGFVVGALSVGIGFGLQNVVSNFVSGIILLIERPISEGDWIEVAGNMGIVKDISVR
STTIETFDKTDVIVPNADFISGTVTNWTRGNTVGRVVLTVGVAYGNDTRRVADLLMEIANGHDDVVSFPAPGVDFLGFGA
DSLDFRVRVILRDINLFVTVQTELYHRINETFVREGIEIPFAQRDVWLRNPETLQPLQSKPEPQPQAGPSTPPQEA

Sequences:

>Translated_796_residues
MTAIVGRLLTALFLALIPVMAAMPGAVVAQTTAPDAEIDYEAWEREAAATEAVIEGARASTSLLETRRLELVRWRAQFLA
SDASNEERIATIQSQIDALGPGPADGDVEPDAIADRRTALGEAMTAAQAPGLQAREAFNRANGLIAEIDTILLERQTQLL
LERDPTPLNPANWAIAINALVEFVRDQQRQKGGLPQDPDSLLSAAETLPLLIFAVVVGLALIVYGRPFVARLGARFLDTD
RRRGRTALGFAVSLGQLLLPLVGFLVIILALDLSGLLTEDLDALAVAVGALILSVYIALWIAGRLFPDHEERAAAFDIQM
PARSQARRTILFIGVFAGLAAFAEAVAAFDVIPPAARGVFILPAYAGLAWMFIRFGRLMRAARAEVEDGDSSNFGQRVVG
LLTRALTVIAVVGPVLVVFGYINAAEAIMLPTVTTLLILGILLVLQSVIRDLYAVLFRSTVEAAADALLPVIVNFSLFVL
ATPLLALVWGVRPERLQEIYARVLEGFTLGDTRITPGIVLAVLLVFAAGVLLTRILQGALKSTVLPRTKLDVGARNAVSA
GVGYVGIALACVIAVTSAGIDLTALGFVVGALSVGIGFGLQNVVSNFVSGIILLIERPISEGDWIEVAGNMGIVKDISVR
STTIETFDKTDVIVPNADFISGTVTNWTRGNTVGRVVLTVGVAYGNDTRRVADLLMEIANGHDDVVSFPAPGVDFLGFGA
DSLDFRVRVILRDINLFVTVQTELYHRINETFVREGIEIPFAQRDVWLRNPETLQPLQSKPEPQPQAGPSTPPQEA
>Mature_795_residues
TAIVGRLLTALFLALIPVMAAMPGAVVAQTTAPDAEIDYEAWEREAAATEAVIEGARASTSLLETRRLELVRWRAQFLAS
DASNEERIATIQSQIDALGPGPADGDVEPDAIADRRTALGEAMTAAQAPGLQAREAFNRANGLIAEIDTILLERQTQLLL
ERDPTPLNPANWAIAINALVEFVRDQQRQKGGLPQDPDSLLSAAETLPLLIFAVVVGLALIVYGRPFVARLGARFLDTDR
RRGRTALGFAVSLGQLLLPLVGFLVIILALDLSGLLTEDLDALAVAVGALILSVYIALWIAGRLFPDHEERAAAFDIQMP
ARSQARRTILFIGVFAGLAAFAEAVAAFDVIPPAARGVFILPAYAGLAWMFIRFGRLMRAARAEVEDGDSSNFGQRVVGL
LTRALTVIAVVGPVLVVFGYINAAEAIMLPTVTTLLILGILLVLQSVIRDLYAVLFRSTVEAAADALLPVIVNFSLFVLA
TPLLALVWGVRPERLQEIYARVLEGFTLGDTRITPGIVLAVLLVFAAGVLLTRILQGALKSTVLPRTKLDVGARNAVSAG
VGYVGIALACVIAVTSAGIDLTALGFVVGALSVGIGFGLQNVVSNFVSGIILLIERPISEGDWIEVAGNMGIVKDISVRS
TTIETFDKTDVIVPNADFISGTVTNWTRGNTVGRVVLTVGVAYGNDTRRVADLLMEIANGHDDVVSFPAPGVDFLGFGAD
SLDFRVRVILRDINLFVTVQTELYHRINETFVREGIEIPFAQRDVWLRNPETLQPLQSKPEPQPQAGPSTPPQEA

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=259, Percent_Identity=35.9073359073359, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI2367355, Length=248, Percent_Identity=32.258064516129, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1789291, Length=251, Percent_Identity=29.0836653386454, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1787591, Length=297, Percent_Identity=24.2424242424242, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 85222; Mature: 85091

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVGRLLTALFLALIPVMAAMPGAVVAQTTAPDAEIDYEAWEREAAATEAVIEGARAS
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
TSLLETRRLELVRWRAQFLASDASNEERIATIQSQIDALGPGPADGDVEPDAIADRRTAL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
GEAMTAAQAPGLQAREAFNRANGLIAEIDTILLERQTQLLLERDPTPLNPANWAIAINAL
HHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VEFVRDQQRQKGGLPQDPDSLLSAAETLPLLIFAVVVGLALIVYGRPFVARLGARFLDTD
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
RRRGRTALGFAVSLGQLLLPLVGFLVIILALDLSGLLTEDLDALAVAVGALILSVYIALW
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGRLFPDHEERAAAFDIQMPARSQARRTILFIGVFAGLAAFAEAVAAFDVIPPAARGVF
HHHCCCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ILPAYAGLAWMFIRFGRLMRAARAEVEDGDSSNFGQRVVGLLTRALTVIAVVGPVLVVFG
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YINAAEAIMLPTVTTLLILGILLVLQSVIRDLYAVLFRSTVEAAADALLPVIVNFSLFVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATPLLALVWGVRPERLQEIYARVLEGFTLGDTRITPGIVLAVLLVFAAGVLLTRILQGAL
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KSTVLPRTKLDVGARNAVSAGVGYVGIALACVIAVTSAGIDLTALGFVVGALSVGIGFGL
HHHCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCH
QNVVSNFVSGIILLIERPISEGDWIEVAGNMGIVKDISVRSTTIETFDKTDVIVPNADFI
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCCCHHHHCCCCCEECCCCCCC
SGTVTNWTRGNTVGRVVLTVGVAYGNDTRRVADLLMEIANGHDDVVSFPAPGVDFLGFGA
CCCCCCCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHCCCC
DSLDFRVRVILRDINLFVTVQTELYHRINETFVREGIEIPFAQRDVWLRNPETLQPLQSK
CCHHHHHHHHEEECCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCHHHCC
PEPQPQAGPSTPPQEA
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAIVGRLLTALFLALIPVMAAMPGAVVAQTTAPDAEIDYEAWEREAAATEAVIEGARAS
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
TSLLETRRLELVRWRAQFLASDASNEERIATIQSQIDALGPGPADGDVEPDAIADRRTAL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
GEAMTAAQAPGLQAREAFNRANGLIAEIDTILLERQTQLLLERDPTPLNPANWAIAINAL
HHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VEFVRDQQRQKGGLPQDPDSLLSAAETLPLLIFAVVVGLALIVYGRPFVARLGARFLDTD
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
RRRGRTALGFAVSLGQLLLPLVGFLVIILALDLSGLLTEDLDALAVAVGALILSVYIALW
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGRLFPDHEERAAAFDIQMPARSQARRTILFIGVFAGLAAFAEAVAAFDVIPPAARGVF
HHHCCCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ILPAYAGLAWMFIRFGRLMRAARAEVEDGDSSNFGQRVVGLLTRALTVIAVVGPVLVVFG
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YINAAEAIMLPTVTTLLILGILLVLQSVIRDLYAVLFRSTVEAAADALLPVIVNFSLFVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATPLLALVWGVRPERLQEIYARVLEGFTLGDTRITPGIVLAVLLVFAAGVLLTRILQGAL
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KSTVLPRTKLDVGARNAVSAGVGYVGIALACVIAVTSAGIDLTALGFVVGALSVGIGFGL
HHHCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCH
QNVVSNFVSGIILLIERPISEGDWIEVAGNMGIVKDISVRSTTIETFDKTDVIVPNADFI
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCCCHHHHCCCCCEECCCCCCC
SGTVTNWTRGNTVGRVVLTVGVAYGNDTRRVADLLMEIANGHDDVVSFPAPGVDFLGFGA
CCCCCCCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHCCCC
DSLDFRVRVILRDINLFVTVQTELYHRINETFVREGIEIPFAQRDVWLRNPETLQPLQSK
CCHHHHHHHHEEECCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCHHHCC
PEPQPQAGPSTPPQEA
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]