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The map label for this gene is ispG

Identifier: 89054426

GI number: 89054426

Start: 1925523

End: 1926656

Strand: Direct

Name: ispG

Synonym: Jann_1935

Alternate gene names: 89054426

Gene position: 1925523-1926656 (Clockwise)

Preceding gene: 89054425

Following gene: 89054427

Centisome position: 44.59

GC content: 62.87

Gene sequence:

>1134_bases
ATGTCCCACAATCCGATCCGCCCCTGGCGCAACATCGACCGGCGCAAGAGCCGCCAGATCATGGTCGGCAACGTGCCGGT
TGGCGGCGACGCACCGATTACCGTGCAGACCATGACCAACACGCTGACCACCGATGCCAGCGCCACGATCAAACAGGTCA
TCGCGGCGGCGGAGGCGGGGGCGGATATCGTCCGTGTCTCTGTGCCGGATGCGGATTCGGCCCGCGCGATGCATGAGATT
TGCCGCGAAAGCCCGGTGCCCATCGTGGCCGACATCCACTTCCATTATAAGCGTGGGATCGAGGCGGCCGAGGCGGGCGC
GGCCTGCCTGCGGATCAATCCCGGCAACATTGGCGACGCGGCCCGTGTGAAAGAGGTCGTGAAGGCGGCGCGGGACCACA
ATTGCTCCATCCGCATCGGCGTGAATGCGGGGTCGCTGGAAAAGCACCTGTTGGAGAAATACGGGGAGCCATGCCCCGAT
GCCATGGTCGAGAGTGGCATGGACCATATCAAGCTGTTGGAAGACAACGACTTTCACGAGTTCAAGATCTCCATGAAAGC
CTCCGACATCTTCATGACTGCCGCCGCCTATCAGCAACTCGCCGACCAGACCGATGCGCCGTTCCACATGGGGATCACGG
AAGCAGGCGGGTTCGTGGGCGGCACGGTGAAATCCGCCATCGGCCTCGGCAACCTGCTTTGGGCGGGGATTGGCGATACG
ATGCGCGTGTCGCTCTCCGCCGATCCGGTGGAGGAGGTGAAGATCGGGTTCGAGATCCTGAAATCCCTTGGCCTGCGGCA
CCGGGGCGTGAACATCATCTCCTGCCCGTCCTGTGCGCGGCAGGGCTTCGACGTGATCAAGACGGTGGAGGTGTTGGAAC
AGCGGCTGGAGCATATCAAGACGCCCATGAGCCTCTCGATCATCGGCTGCGTGGTGAACGGACCGGGAGAGGCGCTGATG
ACCGATGTGGGCTTCACCGGCGGCGGGGCGGGCAGCGGCATGGTCTATCTGGCGGGCAAGCAGAGCCACAAGATGAGCAA
TGAGCAGATGGTCGATCACATCGTCGAACAGGTCGAAAAACGCGCCGAGGCCATTGAGGCCCAGGCCAAAGCGGCAGACC
AGGCCGCAGAATAG

Upstream 100 bases:

>100_bases
GATGCCGGTCTTTTTGCCCCATCTCCCACCGCCCACATTGTCCAGCCCGCCTGACACGTCTATCGTGCTGGTCAGCTGAC
CAAACGCGAGTGCGCGACCC

Downstream 100 bases:

>100_bases
GGAGCACGCGATGAAAACTGCCAAGGATTATATGGACGCTGCCAATGCCTCCGTGCCGAAGCTGTCCGCCGAGGAGGCGA
TCGCCAAACATGGCGCGGGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSHNPIRPWRNIDRRKSRQIMVGNVPVGGDAPITVQTMTNTLTTDASATIKQVIAAAEAGADIVRVSVPDADSARAMHEI
CRESPVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDAARVKEVVKAARDHNCSIRIGVNAGSLEKHLLEKYGEPCPD
AMVESGMDHIKLLEDNDFHEFKISMKASDIFMTAAAYQQLADQTDAPFHMGITEAGGFVGGTVKSAIGLGNLLWAGIGDT
MRVSLSADPVEEVKIGFEILKSLGLRHRGVNIISCPSCARQGFDVIKTVEVLEQRLEHIKTPMSLSIIGCVVNGPGEALM
TDVGFTGGGAGSGMVYLAGKQSHKMSNEQMVDHIVEQVEKRAEAIEAQAKAADQAAE

Sequences:

>Translated_377_residues
MSHNPIRPWRNIDRRKSRQIMVGNVPVGGDAPITVQTMTNTLTTDASATIKQVIAAAEAGADIVRVSVPDADSARAMHEI
CRESPVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDAARVKEVVKAARDHNCSIRIGVNAGSLEKHLLEKYGEPCPD
AMVESGMDHIKLLEDNDFHEFKISMKASDIFMTAAAYQQLADQTDAPFHMGITEAGGFVGGTVKSAIGLGNLLWAGIGDT
MRVSLSADPVEEVKIGFEILKSLGLRHRGVNIISCPSCARQGFDVIKTVEVLEQRLEHIKTPMSLSIIGCVVNGPGEALM
TDVGFTGGGAGSGMVYLAGKQSHKMSNEQMVDHIVEQVEKRAEAIEAQAKAADQAAE
>Mature_376_residues
SHNPIRPWRNIDRRKSRQIMVGNVPVGGDAPITVQTMTNTLTTDASATIKQVIAAAEAGADIVRVSVPDADSARAMHEIC
RESPVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDAARVKEVVKAARDHNCSIRIGVNAGSLEKHLLEKYGEPCPDA
MVESGMDHIKLLEDNDFHEFKISMKASDIFMTAAAYQQLADQTDAPFHMGITEAGGFVGGTVKSAIGLGNLLWAGIGDTM
RVSLSADPVEEVKIGFEILKSLGLRHRGVNIISCPSCARQGFDVIKTVEVLEQRLEHIKTPMSLSIIGCVVNGPGEALMT
DVGFTGGGAGSGMVYLAGKQSHKMSNEQMVDHIVEQVEKRAEAIEAQAKAADQAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=366, Percent_Identity=56.8306010928962, Blast_Score=423, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_JANSC (Q28R10)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509877.1
- ProteinModelPortal:   Q28R10
- STRING:   Q28R10
- GeneID:   3934386
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1935
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   HEICRES
- PhylomeDB:   Q28R10
- ProtClustDB:   PRK00366
- BioCyc:   JSP290400:JANN_1935-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR016425
- InterPro:   IPR004588
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51366 RibP_bind_barrel

EC number: =1.17.7.1

Molecular weight: Translated: 40312; Mature: 40180

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNPIRPWRNIDRRKSRQIMVGNVPVGGDAPITVQTMTNTLTTDASATIKQVIAAAEAG
CCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHCC
ADIVRVSVPDADSARAMHEICRESPVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDA
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCHHHCCCEEEEECCCCCCCH
ARVKEVVKAARDHNCSIRIGVNAGSLEKHLLEKYGEPCPDAMVESGMDHIKLLEDNDFHE
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCCEE
FKISMKASDIFMTAAAYQQLADQTDAPFHMGITEAGGFVGGTVKSAIGLGNLLWAGIGDT
EEEEEEHHHHHHHHHHHHHHHHCCCCCEEECHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
MRVSLSADPVEEVKIGFEILKSLGLRHRGVNIISCPSCARQGFDVIKTVEVLEQRLEHIK
EEEEECCCCHHHHHHHHHHHHHHCCHHCCCCEEECCHHHHHCHHHHHHHHHHHHHHHHHC
TPMSLSIIGCVVNGPGEALMTDVGFTGGGAGSGMVYLAGKQSHKMSNEQMVDHIVEQVEK
CCCEEEEEEEEECCCCCCEEEECCCCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHH
RAEAIEAQAKAADQAAE
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHNPIRPWRNIDRRKSRQIMVGNVPVGGDAPITVQTMTNTLTTDASATIKQVIAAAEAG
CCCCCCCHHHHHHHCCCEEEECCCCCCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHCC
ADIVRVSVPDADSARAMHEICRESPVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDA
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCHHHCCCEEEEECCCCCCCH
ARVKEVVKAARDHNCSIRIGVNAGSLEKHLLEKYGEPCPDAMVESGMDHIKLLEDNDFHE
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCCEE
FKISMKASDIFMTAAAYQQLADQTDAPFHMGITEAGGFVGGTVKSAIGLGNLLWAGIGDT
EEEEEEHHHHHHHHHHHHHHHHCCCCCEEECHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
MRVSLSADPVEEVKIGFEILKSLGLRHRGVNIISCPSCARQGFDVIKTVEVLEQRLEHIK
EEEEECCCCHHHHHHHHHHHHHHCCHHCCCCEEECCHHHHHCHHHHHHHHHHHHHHHHHC
TPMSLSIIGCVVNGPGEALMTDVGFTGGGAGSGMVYLAGKQSHKMSNEQMVDHIVEQVEK
CCCEEEEEEEEECCCCCCEEEECCCCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHH
RAEAIEAQAKAADQAAE
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA