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The map label for this gene is purF [H]

Identifier: 89054198

GI number: 89054198

Start: 1689020

End: 1690513

Strand: Reverse

Name: purF [H]

Synonym: Jann_1707

Alternate gene names: 89054198

Gene position: 1690513-1689020 (Counterclockwise)

Preceding gene: 89054200

Following gene: 89054197

Centisome position: 39.15

GC content: 63.59

Gene sequence:

>1494_bases
ATGGACGAGACAGCCGTCCCCGCCTCGCACTTTCCAAGGCATCCCTTCGACGCCTGCGATGAGGATAAATTGCGCGAGGA
ATGCGGCGTTTTCGGTGTGATCGGCGTGACGGATGCGGCGAATTTCGTGGCCCTTGGCCTGCACGCCCTGCAACATCGCG
GGCAGGAAGCGGGCGGGATCGTCACCCATGACCCCGATGCGGGCTTCAGCTCGGCCCGTCGGTTCGGCTACGTGCGCGAC
ACCTTCACATCGCAAAAGGTGATGGAGACGCTGCCGGGGTCCATCGGGATCGGCCATGTGCGCTATTCCACCGCCGGATC
GAAGGGGGCCACGCAGATCCGCGACGTGCAGCCGTTCTTCGGCGAATTTGCCATGGGCGGCGCGGCGATTGCCCATAACG
GCAACATCGTCAACGCCGATGAGCTGCGCAAAGAGCTGATCGAGCGCGGGTCGATCTTCCAGTCGTCCTCCGACAGTGAA
TGCATCATCCATCTGATGGCCCGGTCCCTGCAACGCAACATCCCGGAGCGGATGAAAGACGGGTTGCGAAGGGTGGAGGG
GGCGTTTTCCATCGTCGCCATGACGCGCACCAAGCTGATCGGCTGCCGCGATGCGCTGGGTGTGCGCCCGCTGGTGATCG
GCAAGTTGGGGGACGGCTATGTGTTGTCGTCGGAGACCTGCGCGCTGGACATCATCGGCGCGGAACTGATCCGGGAGGTG
GAGCCCGGAGAGATGGTGGTGATCACCGCCCAGGGCATCGAATCCTCCAAGCCGTTCAACGCGGCACGGCCCCGGTTCTG
TATCTTCGAGCATGTCTATTTCTCCCGCCCCGACAGCATCATCGGCGGGCAATCCGTCTATGAGACGCGCCGGCAGATCG
GCGTGGAACTGGCGAAAGAGGCCCCGGTGGACGCAGACTTGGTGTGTCCCGTGCCGGATTCAGGGACGCCTGCCGCCATT
GGCTACAGTCAGGAAAGCGGCATCCCCTACGCCATGGGCATCATCCGCAACCAATATATGGGCCGCACGTTCATCGAGCC
ATCCGAGCAGATCCGCAACATGGGCGTGCGCCTGAAGCTGAACGTGAACCGCGCGCTGATCCGCGGCAAGCGGGTGATCC
TGGTGGATGACAGCGTGGTCCGCGGCACGACCAGCCGGAAGATCAAAGAGATGATCCTGGATGCGGGCGCGGCAGAGGTG
CATTTCCGCATCGCCTCCCCCCCCACCGCCTGGCCGTGCTTTTACGGGGTCGACACGCCGGAGCGGAGCAAGCTGCTGGC
CGCGACGATGACGGCGGAAGAGATGCGCGACTACCTTGCCGTCGATAGCCTGAAATTCATTTCGCTCGATGGGCTCTACC
GCGCGGTGGGCGAGGCCAATGGCCGGGATCCGGCGCAGCCTGCCTATTGCGATGCGTGTTTTTCGGGCGAGTATCCCGTC
AGCCCAACCGACCAGATCGCGAAAGGGTTCCAACTGGTGGAGGCGGCTGAATGA

Upstream 100 bases:

>100_bases
CCGCCGGTCCAATCCCCCGTGACAAACGTATGATCGTTTCGTGACACCGCCGCGCGGGCGTCCTATGTGTCACTCACGCT
AAGGATTAAGGATTCGCCCG

Downstream 100 bases:

>100_bases
TCGGCAAATTGTTCCGCCGCGCCAAAGTCGCCCCGGAGATCCCCGAAGACGAGTTGGCCCGCCGCCTGATCGACCCTGAT
TTCATCGTCGCATCCGGCGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MDETAVPASHFPRHPFDACDEDKLREECGVFGVIGVTDAANFVALGLHALQHRGQEAGGIVTHDPDAGFSSARRFGYVRD
TFTSQKVMETLPGSIGIGHVRYSTAGSKGATQIRDVQPFFGEFAMGGAAIAHNGNIVNADELRKELIERGSIFQSSSDSE
CIIHLMARSLQRNIPERMKDGLRRVEGAFSIVAMTRTKLIGCRDALGVRPLVIGKLGDGYVLSSETCALDIIGAELIREV
EPGEMVVITAQGIESSKPFNAARPRFCIFEHVYFSRPDSIIGGQSVYETRRQIGVELAKEAPVDADLVCPVPDSGTPAAI
GYSQESGIPYAMGIIRNQYMGRTFIEPSEQIRNMGVRLKLNVNRALIRGKRVILVDDSVVRGTTSRKIKEMILDAGAAEV
HFRIASPPTAWPCFYGVDTPERSKLLAATMTAEEMRDYLAVDSLKFISLDGLYRAVGEANGRDPAQPAYCDACFSGEYPV
SPTDQIAKGFQLVEAAE

Sequences:

>Translated_497_residues
MDETAVPASHFPRHPFDACDEDKLREECGVFGVIGVTDAANFVALGLHALQHRGQEAGGIVTHDPDAGFSSARRFGYVRD
TFTSQKVMETLPGSIGIGHVRYSTAGSKGATQIRDVQPFFGEFAMGGAAIAHNGNIVNADELRKELIERGSIFQSSSDSE
CIIHLMARSLQRNIPERMKDGLRRVEGAFSIVAMTRTKLIGCRDALGVRPLVIGKLGDGYVLSSETCALDIIGAELIREV
EPGEMVVITAQGIESSKPFNAARPRFCIFEHVYFSRPDSIIGGQSVYETRRQIGVELAKEAPVDADLVCPVPDSGTPAAI
GYSQESGIPYAMGIIRNQYMGRTFIEPSEQIRNMGVRLKLNVNRALIRGKRVILVDDSVVRGTTSRKIKEMILDAGAAEV
HFRIASPPTAWPCFYGVDTPERSKLLAATMTAEEMRDYLAVDSLKFISLDGLYRAVGEANGRDPAQPAYCDACFSGEYPV
SPTDQIAKGFQLVEAAE
>Mature_497_residues
MDETAVPASHFPRHPFDACDEDKLREECGVFGVIGVTDAANFVALGLHALQHRGQEAGGIVTHDPDAGFSSARRFGYVRD
TFTSQKVMETLPGSIGIGHVRYSTAGSKGATQIRDVQPFFGEFAMGGAAIAHNGNIVNADELRKELIERGSIFQSSSDSE
CIIHLMARSLQRNIPERMKDGLRRVEGAFSIVAMTRTKLIGCRDALGVRPLVIGKLGDGYVLSSETCALDIIGAELIREV
EPGEMVVITAQGIESSKPFNAARPRFCIFEHVYFSRPDSIIGGQSVYETRRQIGVELAKEAPVDADLVCPVPDSGTPAAI
GYSQESGIPYAMGIIRNQYMGRTFIEPSEQIRNMGVRLKLNVNRALIRGKRVILVDDSVVRGTTSRKIKEMILDAGAAEV
HFRIASPPTAWPCFYGVDTPERSKLLAATMTAEEMRDYLAVDSLKFISLDGLYRAVGEANGRDPAQPAYCDACFSGEYPV
SPTDQIAKGFQLVEAAE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=513, Percent_Identity=38.7914230019493, Blast_Score=354, Evalue=9e-98,
Organism=Escherichia coli, GI1788651, Length=480, Percent_Identity=38.125, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1790167, Length=211, Percent_Identity=29.8578199052133, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=39.7489539748954, Blast_Score=331, Evalue=5e-91,
Organism=Saccharomyces cerevisiae, GI6323958, Length=455, Percent_Identity=35.6043956043956, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24659598, Length=494, Percent_Identity=41.2955465587045, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=41.8454935622318, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI28573187, Length=501, Percent_Identity=40.5189620758483, Blast_Score=360, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54041; Mature: 54041

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDETAVPASHFPRHPFDACDEDKLREECGVFGVIGVTDAANFVALGLHALQHRGQEAGGI
CCCCCCCHHHCCCCCCCCCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCE
VTHDPDAGFSSARRFGYVRDTFTSQKVMETLPGSIGIGHVRYSTAGSKGATQIRDVQPFF
EECCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHCCHHH
GEFAMGGAAIAHNGNIVNADELRKELIERGSIFQSSSDSECIIHLMARSLQRNIPERMKD
HHHHCCCEEEECCCCEECHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
GLRRVEGAFSIVAMTRTKLIGCRDALGVRPLVIGKLGDGYVLSSETCALDIIGAELIREV
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCEEECCCCCEEEHHHHHHHHHC
EPGEMVVITAQGIESSKPFNAARPRFCIFEHVYFSRPDSIIGGQSVYETRRQIGVELAKE
CCCCEEEEEECCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
APVDADLVCPVPDSGTPAAIGYSQESGIPYAMGIIRNQYMGRTFIEPSEQIRNMGVRLKL
CCCCCCEEECCCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEE
NVNRALIRGKRVILVDDSVVRGTTSRKIKEMILDAGAAEVHFRIASPPTAWPCFYGVDTP
ECCHHHHCCCEEEEECCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCC
ERSKLLAATMTAEEMRDYLAVDSLKFISLDGLYRAVGEANGRDPAQPAYCDACFSGEYPV
CHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCC
SPTDQIAKGFQLVEAAE
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDETAVPASHFPRHPFDACDEDKLREECGVFGVIGVTDAANFVALGLHALQHRGQEAGGI
CCCCCCCHHHCCCCCCCCCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCE
VTHDPDAGFSSARRFGYVRDTFTSQKVMETLPGSIGIGHVRYSTAGSKGATQIRDVQPFF
EECCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHCCHHH
GEFAMGGAAIAHNGNIVNADELRKELIERGSIFQSSSDSECIIHLMARSLQRNIPERMKD
HHHHCCCEEEECCCCEECHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
GLRRVEGAFSIVAMTRTKLIGCRDALGVRPLVIGKLGDGYVLSSETCALDIIGAELIREV
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCEEECCCCCEEEHHHHHHHHHC
EPGEMVVITAQGIESSKPFNAARPRFCIFEHVYFSRPDSIIGGQSVYETRRQIGVELAKE
CCCCEEEEEECCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
APVDADLVCPVPDSGTPAAIGYSQESGIPYAMGIIRNQYMGRTFIEPSEQIRNMGVRLKL
CCCCCCEEECCCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEE
NVNRALIRGKRVILVDDSVVRGTTSRKIKEMILDAGAAEVHFRIASPPTAWPCFYGVDTP
ECCHHHHCCCEEEEECCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCC
ERSKLLAATMTAEEMRDYLAVDSLKFISLDGLYRAVGEANGRDPAQPAYCDACFSGEYPV
CHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCC
SPTDQIAKGFQLVEAAE
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA