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The map label for this gene is pdhB [H]

Identifier: 89054181

GI number: 89054181

Start: 1672386

End: 1673780

Strand: Reverse

Name: pdhB [H]

Synonym: Jann_1690

Alternate gene names: 89054181

Gene position: 1673780-1672386 (Counterclockwise)

Preceding gene: 89054182

Following gene: 89054180

Centisome position: 38.76

GC content: 63.23

Gene sequence:

>1395_bases
ATGGCTGTAGAAATTCTGATGCCCGCCCTGTCCCCCACGATGGAAGAGGGCACCCTGGCCAAGTGGCTGGTCAAGGAAGG
CGACACCGTTCAGTCCGGCGACATCCTGGCCGAGATCGAGACCGACAAGGCCACCATGGAGTTCGAGGCCGTTGATGAAG
GCGTCATCGGCAAGATCCTGATCGAGGAAGGCACCGAAGGCGTGAAGGTCAACACCGCCATCGCCATTATTGGCGAGGAA
GGTGAGGACATGTCCTCCGCGTCCGCAGCGCCCGCCTCAGATGCAAGCGCTGAGGAGGCCGCGCCAGCGGACTCCTCCAC
CGCCGAGGAAGAAGCCCCTGCCTCAGCCGCGCAAGCGGCGGCCCCGGCCGCGCCCAAGGCGGACACAAGCCCCGACTGGC
CCGAAGGGACAGCGATGAAAACCCAGACGGTGCGCGAAGCGCTTCGCGACGCGATGTCCGAGGAAATGCGCGCCGATGAG
GCCGTCTTTGTAATGGGCGAAGAGGTCGCCGAATATAACGGCGCCTACAAGATCACCCAGGGCATGCTGGACGAATTTGG
CGACAAGCGCGTCATCGACACGCCCATTACCGAGCACGGCTTTGCCGGGATCGGTGTGGGCGCGGCCTTCGGCGGCCTCA
AGCCCATCGTGGAGTTCATGACCTTCAACTTCGCCATGCAGGCGATCGACCACATCATCAACTCCGCCGCCAAGACGCTC
TATATGTCCGGCGGCCAGATGGGCGCGCCCATGGTGTTCCGGGGTCCCAACGGGGCCGCCGCCCGCGTGGGCGCGCAGCA
TTCCCAGGATTACGCGGCGTGGTACAGCCAGATCCCCGGCCTGAAGGTCGTGATGCCCTATTCCGCCGCCGACGCCAAAG
GCTTGCTGAAAACCGCCATCCGCGACCCCAACCCGGTGATTTTCCTGGAAAACGAGATCCTTTATGGCCGCTCTTTCGAC
GTGCCGGATATGGACGATTTCACAATCCCCTTCGGCAAGGCCAAGATCTGGCGCGAGGGCGATGACGTCACCCTCGTCTC
CTTCGGGATCGGCATGACCTACGCCTTGGAGGCCGCCGAAAAGCTGGCAGAAGAGGGTATTAGCGCCGAGGTCATCGACC
TGCGCACCCTGCGTCCGCTGGACTACGACACGCTGCTGGCCTCGGTCATGAAGACCAACCGCTGCGTGACCGTGGAGGAA
GGCTTCCCCGTCTGTTCCATCGGCAACCACCTGTCGGCCTACCTGATGCAAAACGCGTTCGACTACCTCGACGCGCCGGT
CATCAATTGCACCGGCAAGGACGTGCCCATGCCTTACGCGGCCAACCTGGAACGCCACGCGCTGATCACGACGGATGAAG
TCGTGGACGCGGTCAAACAGGTCACCTACCGCTGA

Upstream 100 bases:

>100_bases
CGCCTCCGCCGAGTTCGCCAAGGAAAGCCCGGAGCCCGCGTTAGAAGAGCTGTGGACCGACATCTACGCAAAAGAAATCC
CGCAGGGGGAGGCTGTATAA

Downstream 100 bases:

>100_bases
TGCGTATCCTAGGCAAAAACGGCTCCGCCGACGCCTACCGCGTCTCCGTCGATCTCGGCGGGGATGGGGTGGTGGCGCTG
GTCCCCGATGCCCTTTTGTC

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MAVEILMPALSPTMEEGTLAKWLVKEGDTVQSGDILAEIETDKATMEFEAVDEGVIGKILIEEGTEGVKVNTAIAIIGEE
GEDMSSASAAPASDASAEEAAPADSSTAEEEAPASAAQAAAPAAPKADTSPDWPEGTAMKTQTVREALRDAMSEEMRADE
AVFVMGEEVAEYNGAYKITQGMLDEFGDKRVIDTPITEHGFAGIGVGAAFGGLKPIVEFMTFNFAMQAIDHIINSAAKTL
YMSGGQMGAPMVFRGPNGAAARVGAQHSQDYAAWYSQIPGLKVVMPYSAADAKGLLKTAIRDPNPVIFLENEILYGRSFD
VPDMDDFTIPFGKAKIWREGDDVTLVSFGIGMTYALEAAEKLAEEGISAEVIDLRTLRPLDYDTLLASVMKTNRCVTVEE
GFPVCSIGNHLSAYLMQNAFDYLDAPVINCTGKDVPMPYAANLERHALITTDEVVDAVKQVTYR

Sequences:

>Translated_464_residues
MAVEILMPALSPTMEEGTLAKWLVKEGDTVQSGDILAEIETDKATMEFEAVDEGVIGKILIEEGTEGVKVNTAIAIIGEE
GEDMSSASAAPASDASAEEAAPADSSTAEEEAPASAAQAAAPAAPKADTSPDWPEGTAMKTQTVREALRDAMSEEMRADE
AVFVMGEEVAEYNGAYKITQGMLDEFGDKRVIDTPITEHGFAGIGVGAAFGGLKPIVEFMTFNFAMQAIDHIINSAAKTL
YMSGGQMGAPMVFRGPNGAAARVGAQHSQDYAAWYSQIPGLKVVMPYSAADAKGLLKTAIRDPNPVIFLENEILYGRSFD
VPDMDDFTIPFGKAKIWREGDDVTLVSFGIGMTYALEAAEKLAEEGISAEVIDLRTLRPLDYDTLLASVMKTNRCVTVEE
GFPVCSIGNHLSAYLMQNAFDYLDAPVINCTGKDVPMPYAANLERHALITTDEVVDAVKQVTYR
>Mature_463_residues
AVEILMPALSPTMEEGTLAKWLVKEGDTVQSGDILAEIETDKATMEFEAVDEGVIGKILIEEGTEGVKVNTAIAIIGEEG
EDMSSASAAPASDASAEEAAPADSSTAEEEAPASAAQAAAPAAPKADTSPDWPEGTAMKTQTVREALRDAMSEEMRADEA
VFVMGEEVAEYNGAYKITQGMLDEFGDKRVIDTPITEHGFAGIGVGAAFGGLKPIVEFMTFNFAMQAIDHIINSAAKTLY
MSGGQMGAPMVFRGPNGAAARVGAQHSQDYAAWYSQIPGLKVVMPYSAADAKGLLKTAIRDPNPVIFLENEILYGRSFDV
PDMDDFTIPFGKAKIWREGDDVTLVSFGIGMTYALEAAEKLAEEGISAEVIDLRTLRPLDYDTLLASVMKTNRCVTVEEG
FPVCSIGNHLSAYLMQNAFDYLDAPVINCTGKDVPMPYAANLERHALITTDEVVDAVKQVTYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=332, Percent_Identity=55.421686746988, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI291084858, Length=332, Percent_Identity=52.1084337349398, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI4557353, Length=300, Percent_Identity=36, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI34101272, Length=300, Percent_Identity=36, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI203098753, Length=81, Percent_Identity=54.320987654321, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=54.320987654321, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI31711992, Length=84, Percent_Identity=50, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI260898739, Length=58, Percent_Identity=58.6206896551724, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538422, Length=327, Percent_Identity=62.691131498471, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17506935, Length=332, Percent_Identity=39.1566265060241, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=144, Percent_Identity=42.3611111111111, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17539652, Length=445, Percent_Identity=26.7415730337079, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319698, Length=358, Percent_Identity=56.7039106145251, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324258, Length=138, Percent_Identity=41.304347826087, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321632, Length=126, Percent_Identity=34.9206349206349, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21358145, Length=348, Percent_Identity=56.3218390804598, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24650940, Length=348, Percent_Identity=56.3218390804598, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI160714832, Length=302, Percent_Identity=34.4370860927152, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI160714828, Length=302, Percent_Identity=34.4370860927152, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24650943, Length=118, Percent_Identity=55.9322033898305, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24650945, Length=118, Percent_Identity=55.9322033898305, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI20129315, Length=75, Percent_Identity=53.3333333333333, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24582497, Length=64, Percent_Identity=53.125, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 49613; Mature: 49482

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEILMPALSPTMEEGTLAKWLVKEGDTVQSGDILAEIETDKATMEFEAVDEGVIGKIL
CCCEEEEHHCCCCCCCCHHHHHHHHCCCCEECCCEEEEEECCCCEEEEHHCCCCCEEEEE
IEEGTEGVKVNTAIAIIGEEGEDMSSASAAPASDASAEEAAPADSSTAEEEAPASAAQAA
EECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHCCHHHHHHC
APAAPKADTSPDWPEGTAMKTQTVREALRDAMSEEMRADEAVFVMGEEVAEYNGAYKITQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCEEEHH
GMLDEFGDKRVIDTPITEHGFAGIGVGAAFGGLKPIVEFMTFNFAMQAIDHIINSAAKTL
HHHHHCCCCEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
YMSGGQMGAPMVFRGPNGAAARVGAQHSQDYAAWYSQIPGLKVVMPYSAADAKGLLKTAI
EECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
RDPNPVIFLENEILYGRSFDVPDMDDFTIPFGKAKIWREGDDVTLVSFGIGMTYALEAAE
CCCCCEEEEECEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHH
KLAEEGISAEVIDLRTLRPLDYDTLLASVMKTNRCVTVEEGFPVCSIGNHLSAYLMQNAF
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCEEECCHHHHHHHHHHHH
DYLDAPVINCTGKDVPMPYAANLERHALITTDEVVDAVKQVTYR
HHHCCCEEECCCCCCCCCHHCCCCCCEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVEILMPALSPTMEEGTLAKWLVKEGDTVQSGDILAEIETDKATMEFEAVDEGVIGKIL
CCEEEEHHCCCCCCCCHHHHHHHHCCCCEECCCEEEEEECCCCEEEEHHCCCCCEEEEE
IEEGTEGVKVNTAIAIIGEEGEDMSSASAAPASDASAEEAAPADSSTAEEEAPASAAQAA
EECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHCCHHHHHHC
APAAPKADTSPDWPEGTAMKTQTVREALRDAMSEEMRADEAVFVMGEEVAEYNGAYKITQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCEEEHH
GMLDEFGDKRVIDTPITEHGFAGIGVGAAFGGLKPIVEFMTFNFAMQAIDHIINSAAKTL
HHHHHCCCCEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
YMSGGQMGAPMVFRGPNGAAARVGAQHSQDYAAWYSQIPGLKVVMPYSAADAKGLLKTAI
EECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
RDPNPVIFLENEILYGRSFDVPDMDDFTIPFGKAKIWREGDDVTLVSFGIGMTYALEAAE
CCCCCEEEEECEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHH
KLAEEGISAEVIDLRTLRPLDYDTLLASVMKTNRCVTVEEGFPVCSIGNHLSAYLMQNAF
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCEEECCHHHHHHHHHHHH
DYLDAPVINCTGKDVPMPYAANLERHALITTDEVVDAVKQVTYR
HHHCCCEEECCCCCCCCCHHCCCCCCEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]