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The map label for this gene is mdlB [H]

Identifier: 89053894

GI number: 89053894

Start: 1372295

End: 1373401

Strand: Direct

Name: mdlB [H]

Synonym: Jann_1403

Alternate gene names: 89053894

Gene position: 1372295-1373401 (Clockwise)

Preceding gene: 89053893

Following gene: 89053897

Centisome position: 31.78

GC content: 64.05

Gene sequence:

>1107_bases
ATGATCCATTCAAGTGCAGACGCGCGACGCCTGGCGAAGCGGCGTCTGCCCTGGATGGTGTTCGATTACATCGACGGTGC
CGCCGGGGCCGAGACGGGGGCCGCGCGGGGACGGGCGGCCTTCGATACCTTGGAGTTGCGGCCCCGGATTTTGCGCGATG
TCAGTGCGCGCGATCTTGGCGCATCGGTCTTTGGGCAGGCAACGAAGGCCCCATTCGGCATTAGCCCCATGGGCATGTGC
AATCTGTCGGGCCCCGGCGCTGACATGATGCTGGCGCGGTTGGCAGCCCGCGAAGGCGTGCCATTGGGGGTGTCGACTGT
TGCGTCGACCGCCATGGAGCCGTTGATCGAGGAGGCGGAGGGCAATGCCTGGTTCCAGCTTTACTTCACCGGCGACGGGT
CCGGTACGTTCAAACTGGTAGAGCGCGCAAAGTCCGCTGGATATCAGACGCTTATCCTGACGGTGGACGTTCCCGAAGTG
GGCCGCCGACCGCGCGAATTGCGCCACGGCTTCACCATGCCGTTCAGGATCGGCCCCAAGCAGTTCCTTGATTTCGCGCT
GCACCCGCGGTGGTCAATTTCGTCGCTGCTGGCGGGCAAGCCCGACATGGCGAATTTCCAGATGGAGGGGTTTGAGTTCG
ACCGCACAGCCTCCCGCGCGAAGGCGGATTTCGGCACGTTGGCCAGGCTGCGAGAGATGTGGCCCGGCAAGCTGGTGATC
AAAGGCGTGCTAGACCCGGAGGACGCGCGGCTGCTGAAAGAGGCGGGCGTGGATGCGATCCAAGTGTCCAGCCACGGCGC
GCGGCAGTTGGAAAGCGCGCCGGTCCCGATCAGCGTCTTGCCCGCGATCCGGGATGCGGTTGGTCCGGAATTCCCGCTGT
TTTTTGATACCGGCCTGCGTGGCGGAGAGGACGTCGTGAAGGCCTATGCCCAGGGTGCAAACTTCACCTTCCTGGGCCGC
GTGCTTCAATTCGCCATCGCAGCGGGGGGAGAGGAGGGATTGGCTCAGCTGTGGTCTGTGTTGAAAGACGAGACAAGTAT
CACGCTGGCGCAGATCGGCGCGACGTCGCTGGGGCACAATCAGTTGGCCGACGCTATCCGGGCCTAA

Upstream 100 bases:

>100_bases
TCGTGTCCAAATGCGACAGCGGCAACATCAAGCTGCCCGTCATCACCATGGATCCGGTCGTGATCCGCGCCCGCTTCATG
GACGCGGTGGCAAGCTGACG

Downstream 100 bases:

>100_bases
GGACGGGGGTCGTCCAGATCCACCGGAAAGCGGCGTGGCGTCAAGCCCGCGTGAAACCATGTCAGAAGGCTGTAGACGGA
ATAGACCGGAAGCCCGCTTC

Product: FMN-dependent alpha-hydroxy acid dehydrogenase

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MIHSSADARRLAKRRLPWMVFDYIDGAAGAETGAARGRAAFDTLELRPRILRDVSARDLGASVFGQATKAPFGISPMGMC
NLSGPGADMMLARLAAREGVPLGVSTVASTAMEPLIEEAEGNAWFQLYFTGDGSGTFKLVERAKSAGYQTLILTVDVPEV
GRRPRELRHGFTMPFRIGPKQFLDFALHPRWSISSLLAGKPDMANFQMEGFEFDRTASRAKADFGTLARLREMWPGKLVI
KGVLDPEDARLLKEAGVDAIQVSSHGARQLESAPVPISVLPAIRDAVGPEFPLFFDTGLRGGEDVVKAYAQGANFTFLGR
VLQFAIAAGGEEGLAQLWSVLKDETSITLAQIGATSLGHNQLADAIRA

Sequences:

>Translated_368_residues
MIHSSADARRLAKRRLPWMVFDYIDGAAGAETGAARGRAAFDTLELRPRILRDVSARDLGASVFGQATKAPFGISPMGMC
NLSGPGADMMLARLAAREGVPLGVSTVASTAMEPLIEEAEGNAWFQLYFTGDGSGTFKLVERAKSAGYQTLILTVDVPEV
GRRPRELRHGFTMPFRIGPKQFLDFALHPRWSISSLLAGKPDMANFQMEGFEFDRTASRAKADFGTLARLREMWPGKLVI
KGVLDPEDARLLKEAGVDAIQVSSHGARQLESAPVPISVLPAIRDAVGPEFPLFFDTGLRGGEDVVKAYAQGANFTFLGR
VLQFAIAAGGEEGLAQLWSVLKDETSITLAQIGATSLGHNQLADAIRA
>Mature_368_residues
MIHSSADARRLAKRRLPWMVFDYIDGAAGAETGAARGRAAFDTLELRPRILRDVSARDLGASVFGQATKAPFGISPMGMC
NLSGPGADMMLARLAAREGVPLGVSTVASTAMEPLIEEAEGNAWFQLYFTGDGSGTFKLVERAKSAGYQTLILTVDVPEV
GRRPRELRHGFTMPFRIGPKQFLDFALHPRWSISSLLAGKPDMANFQMEGFEFDRTASRAKADFGTLARLREMWPGKLVI
KGVLDPEDARLLKEAGVDAIQVSSHGARQLESAPVPISVLPAIRDAVGPEFPLFFDTGLRGGEDVVKAYAQGANFTFLGR
VLQFAIAAGGEEGLAQLWSVLKDETSITLAQIGATSLGHNQLADAIRA

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI11068137, Length=357, Percent_Identity=33.0532212885154, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI54234014, Length=352, Percent_Identity=32.1022727272727, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI7705393, Length=352, Percent_Identity=32.1022727272727, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI1790033, Length=372, Percent_Identity=33.6021505376344, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI193208036, Length=342, Percent_Identity=30.7017543859649, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32566217, Length=297, Percent_Identity=28.956228956229, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323587, Length=371, Percent_Identity=28.0323450134771, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI78707188, Length=356, Percent_Identity=33.7078651685393, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI281363140, Length=356, Percent_Identity=33.7078651685393, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI78707190, Length=356, Percent_Identity=33.7078651685393, Blast_Score=172, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 39633; Mature: 39633

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHSSADARRLAKRRLPWMVFDYIDGAAGAETGAARGRAAFDTLELRPRILRDVSARDLG
CCCCCHHHHHHHHHCCCEEEEEHHCCCCCCCCCCCCCCHHHHHHHHCHHHHHHCHHHHCC
ASVFGQATKAPFGISPMGMCNLSGPGADMMLARLAAREGVPLGVSTVASTAMEPLIEEAE
HHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
GNAWFQLYFTGDGSGTFKLVERAKSAGYQTLILTVDVPEVGRRPRELRHGFTMPFRIGPK
CCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCHHHCCCHHHHHCCCCCCEECCHH
QFLDFALHPRWSISSLLAGKPDMANFQMEGFEFDRTASRAKADFGTLARLREMWPGKLVI
HHHHHHCCCCCCHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
KGVLDPEDARLLKEAGVDAIQVSSHGARQLESAPVPISVLPAIRDAVGPEFPLFFDTGLR
EECCCCHHHHHHHHCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCC
GGEDVVKAYAQGANFTFLGRVLQFAIAAGGEEGLAQLWSVLKDETSITLAQIGATSLGHN
CHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEHHHHCCCCCCHH
QLADAIRA
HHHHHHCC
>Mature Secondary Structure
MIHSSADARRLAKRRLPWMVFDYIDGAAGAETGAARGRAAFDTLELRPRILRDVSARDLG
CCCCCHHHHHHHHHCCCEEEEEHHCCCCCCCCCCCCCCHHHHHHHHCHHHHHHCHHHHCC
ASVFGQATKAPFGISPMGMCNLSGPGADMMLARLAAREGVPLGVSTVASTAMEPLIEEAE
HHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
GNAWFQLYFTGDGSGTFKLVERAKSAGYQTLILTVDVPEVGRRPRELRHGFTMPFRIGPK
CCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCHHHCCCHHHHHCCCCCCEECCHH
QFLDFALHPRWSISSLLAGKPDMANFQMEGFEFDRTASRAKADFGTLARLREMWPGKLVI
HHHHHHCCCCCCHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
KGVLDPEDARLLKEAGVDAIQVSSHGARQLESAPVPISVLPAIRDAVGPEFPLFFDTGLR
EECCCCHHHHHHHHCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCC
GGEDVVKAYAQGANFTFLGRVLQFAIAAGGEEGLAQLWSVLKDETSITLAQIGATSLGHN
CHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEHHHHCCCCCCHH
QLADAIRA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]