Click here to switch to the map view.

The map label for this gene is tolB

Identifier: 89053466

GI number: 89053466

Start: 930772

End: 932109

Strand: Direct

Name: tolB

Synonym: Jann_0975

Alternate gene names: 89053466

Gene position: 930772-932109 (Clockwise)

Preceding gene: 89053465

Following gene: 89053467

Centisome position: 21.56

GC content: 64.57

Gene sequence:

>1338_bases
ATGTTGAACCGCCGCAATTTCATTCGCACAACCTCGGCCCTTGCGGCCTCCACCGCATTGCCGGGATACGCGTTCGGGCA
ATCGGGCCCGTTGCGGTTGGAGATCACCGAAGGTGTGATCGAGCCGTTGCCCTTCGCCTTGCCGACCTTTCTGGCGGTGG
GCGGAGCCGAAGATGCAGCCGCCGATGTCAGCCGCGTGATCGCAGCGGATCTGCGGGGCACCGGCCTGTTCCGCCAGATC
CCCCCGGATGCCTACATCAGCCAGATCACCAATTTCGGCGCCCCCGTGGCCTATCCCGATTGGCAGGCGATCAACGCGCA
GGCCCTGATCACGGGTCAGGTGGAGGCCCGCGCCGATGGCCAGCTGGTCGTCCGGTTCCGCTTGTTCGACGTGTTCAGCC
AAGCGCCCCTGGGCGAGGGCCTGCAATTTGTCGGTCCGGCGGATAGCTGGCGGCGCATGGCCCACACCGTCGCCGATCAG
GTCTACAGCCGGATCACCGGGGAGGGCGGCTATTTCGACACCCGCGTCGCCTTCATCGCCGAGGAAGGGCCCAAGAACGC
GCGTCTCAAACGGCTTGCGATCATGGATTACGATGGCGCGAATGTGCAGTTCCTGACGGACAGTCGGTCCATCGTATTGG
CCCCGCGCTTCTCGCCCCAAGGCGACAGGATCCTTTACACCAGTTACGAGAGTGGCTTTCCACAGGTCTACATCATGGAT
GTGGCGACCGTGCAGCGCCGCCCGCTGGAGGCGATCCCGGCAGAGACGATGACCTTTTCGCCGCGCTTCAGCCCCTCGGG
GCAATCGGTGGTGTTCAGCCTCGTGGATGGGTCGAACACCGATATCTATTCGCTGGATCTGGCGACGGGCACACGGCGAC
AACTGACCCAGGCCCCGTCGATCGAGACGGCCCCGTCGTTCAGTCCGGACGGCAGCCAGATCGTGTTTGAAAGCGACCGC
TCCGGCAACCAACAGATCTATATCATGCCAGCGGATGGCGGCGAGGCGCGGCGTGTTTCAGCCGGGACGGGGCGCTATGG
CACGCCGGTCTGGTCGCCGCGTGGCGACTACATCGCGTTCACCAAGCAGGAAAACGGGCGCTTCCACATTGGCGTGATGC
GCACCGACGGGTCAGATGAACGGCTTCTGACGTCCTCGTTCCTGGATGAAGGCCCGACCTGGGCGCCCAATGGACGCGTG
ATCATGTTCACCCGCGAAACACCGGGCGATGACGGGGCTCCGGCCGTCTACTCCGTCGATATCTCGGGCCGGAACCTGCA
ACGGGTCGCGACACCGGGCATGGCGTCGGACCCCGCGTGGTCGCCCTTACAGAGTTGA

Upstream 100 bases:

>100_bases
GCCACCGCATCGGCCACTCTTGTTTTTGATCGCAGTGGGGTGCGTTTGCAATGACCCCAGGCGCATCCTACGTATTTGCC
CTTAGTTCAGGAGGTTTCCC

Downstream 100 bases:

>100_bases
ATTGGCGGGGGCCGCGCGCCCGTGCTATACCTTTAAAAAACGAAAGATCCGAGGATCAGAGCTCATGACATTTTCCTTTC
TGAAACCAACCCTTCTGGTA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MLNRRNFIRTTSALAASTALPGYAFGQSGPLRLEITEGVIEPLPFALPTFLAVGGAEDAAADVSRVIAADLRGTGLFRQI
PPDAYISQITNFGAPVAYPDWQAINAQALITGQVEARADGQLVVRFRLFDVFSQAPLGEGLQFVGPADSWRRMAHTVADQ
VYSRITGEGGYFDTRVAFIAEEGPKNARLKRLAIMDYDGANVQFLTDSRSIVLAPRFSPQGDRILYTSYESGFPQVYIMD
VATVQRRPLEAIPAETMTFSPRFSPSGQSVVFSLVDGSNTDIYSLDLATGTRRQLTQAPSIETAPSFSPDGSQIVFESDR
SGNQQIYIMPADGGEARRVSAGTGRYGTPVWSPRGDYIAFTKQENGRFHIGVMRTDGSDERLLTSSFLDEGPTWAPNGRV
IMFTRETPGDDGAPAVYSVDISGRNLQRVATPGMASDPAWSPLQS

Sequences:

>Translated_445_residues
MLNRRNFIRTTSALAASTALPGYAFGQSGPLRLEITEGVIEPLPFALPTFLAVGGAEDAAADVSRVIAADLRGTGLFRQI
PPDAYISQITNFGAPVAYPDWQAINAQALITGQVEARADGQLVVRFRLFDVFSQAPLGEGLQFVGPADSWRRMAHTVADQ
VYSRITGEGGYFDTRVAFIAEEGPKNARLKRLAIMDYDGANVQFLTDSRSIVLAPRFSPQGDRILYTSYESGFPQVYIMD
VATVQRRPLEAIPAETMTFSPRFSPSGQSVVFSLVDGSNTDIYSLDLATGTRRQLTQAPSIETAPSFSPDGSQIVFESDR
SGNQQIYIMPADGGEARRVSAGTGRYGTPVWSPRGDYIAFTKQENGRFHIGVMRTDGSDERLLTSSFLDEGPTWAPNGRV
IMFTRETPGDDGAPAVYSVDISGRNLQRVATPGMASDPAWSPLQS
>Mature_445_residues
MLNRRNFIRTTSALAASTALPGYAFGQSGPLRLEITEGVIEPLPFALPTFLAVGGAEDAAADVSRVIAADLRGTGLFRQI
PPDAYISQITNFGAPVAYPDWQAINAQALITGQVEARADGQLVVRFRLFDVFSQAPLGEGLQFVGPADSWRRMAHTVADQ
VYSRITGEGGYFDTRVAFIAEEGPKNARLKRLAIMDYDGANVQFLTDSRSIVLAPRFSPQGDRILYTSYESGFPQVYIMD
VATVQRRPLEAIPAETMTFSPRFSPSGQSVVFSLVDGSNTDIYSLDLATGTRRQLTQAPSIETAPSFSPDGSQIVFESDR
SGNQQIYIMPADGGEARRVSAGTGRYGTPVWSPRGDYIAFTKQENGRFHIGVMRTDGSDERLLTSSFLDEGPTWAPNGRV
IMFTRETPGDDGAPAVYSVDISGRNLQRVATPGMASDPAWSPLQS

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=423, Percent_Identity=30.0236406619385, Blast_Score=167, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_JANSC (Q28TS0)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508917.1
- ProteinModelPortal:   Q28TS0
- SMR:   Q28TS0
- STRING:   Q28TS0
- GeneID:   3933419
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0975
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q28TS0
- ProtClustDB:   PRK05137
- BioCyc:   JSP290400:JANN_0975-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR006311
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 48276; Mature: 48276

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRRNFIRTTSALAASTALPGYAFGQSGPLRLEITEGVIEPLPFALPTFLAVGGAEDAA
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHEECCCCHHH
ADVSRVIAADLRGTGLFRQIPPDAYISQITNFGAPVAYPDWQAINAQALITGQVEARADG
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCC
QLVVRFRLFDVFSQAPLGEGLQFVGPADSWRRMAHTVADQVYSRITGEGGYFDTRVAFIA
EEEEEEEEEHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
EEGPKNARLKRLAIMDYDGANVQFLTDSRSIVLAPRFSPQGDRILYTSYESGFPQVYIMD
CCCCCCCCEEEEEEEECCCCCEEEEECCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEE
VATVQRRPLEAIPAETMTFSPRFSPSGQSVVFSLVDGSNTDIYSLDLATGTRRQLTQAPS
EEHHCCCCHHHCCCCCEEECCCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHCCCC
IETAPSFSPDGSQIVFESDRSGNQQIYIMPADGGEARRVSAGTGRYGTPVWSPRGDYIAF
CCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEE
TKQENGRFHIGVMRTDGSDERLLTSSFLDEGPTWAPNGRVIMFTRETPGDDGAPAVYSVD
EECCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEE
ISGRNLQRVATPGMASDPAWSPLQS
CCCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MLNRRNFIRTTSALAASTALPGYAFGQSGPLRLEITEGVIEPLPFALPTFLAVGGAEDAA
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHEECCCCHHH
ADVSRVIAADLRGTGLFRQIPPDAYISQITNFGAPVAYPDWQAINAQALITGQVEARADG
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCC
QLVVRFRLFDVFSQAPLGEGLQFVGPADSWRRMAHTVADQVYSRITGEGGYFDTRVAFIA
EEEEEEEEEHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
EEGPKNARLKRLAIMDYDGANVQFLTDSRSIVLAPRFSPQGDRILYTSYESGFPQVYIMD
CCCCCCCCEEEEEEEECCCCCEEEEECCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEE
VATVQRRPLEAIPAETMTFSPRFSPSGQSVVFSLVDGSNTDIYSLDLATGTRRQLTQAPS
EEHHCCCCHHHCCCCCEEECCCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHCCCC
IETAPSFSPDGSQIVFESDRSGNQQIYIMPADGGEARRVSAGTGRYGTPVWSPRGDYIAF
CCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEE
TKQENGRFHIGVMRTDGSDERLLTSSFLDEGPTWAPNGRVIMFTRETPGDDGAPAVYSVD
EECCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEE
ISGRNLQRVATPGMASDPAWSPLQS
CCCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA