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The map label for this gene is hisS

Identifier: 89053436

GI number: 89053436

Start: 902524

End: 904041

Strand: Direct

Name: hisS

Synonym: Jann_0945

Alternate gene names: 89053436

Gene position: 902524-904041 (Clockwise)

Preceding gene: 89053434

Following gene: 89053437

Centisome position: 20.9

GC content: 62.32

Gene sequence:

>1518_bases
ATGGCCAAGCAGAAAAAACAGCCCCGCCCCAAGGCCGAAACGCCCAAGGGGTTCCGCGACTACTTCGGTGCAGAAGTGGT
GGAGCGTCAGACGATGCTCCGCAAGATCGGAGAGGTTTACCACCGCTATGGCTTCGATCCGCTGGAAACGAGCGCTGTGG
AAACGGTGGAGGCCCTTGGCAAGTTCCTCCCAGATGTGGACCGGCCGAATGAGGGGGTTTTTGCGTTTCAGGAAGATGGG
GGATCGGACGACGGCAAATGGCTGGCGCTCCGCTATGACATGACCGCGCCGCTGGCCCGGGTGGCGGCGCAGTTTCGCGA
AAGGCCCCTGCCAGGACAAGAGGACTCTCTGCCCTCGCCCTATCGCCGCTATACTATGGGACCGGTCTGGCGGAATGAGA
AGCCCGGCCCGGGGCGCTTCCGGCAATTCTACCAATGCGACGCGGACACGGTCGGCGCGCCCTCCGTCGCCGCCGACGCT
GAGATCTGTGCGATGCTGGCCGATTGCCTTGAAGCCGTGGGCATTGAGCGCGGCGACTATGTTGTGCGCGTGAACAACCG
CAAGGTGCTGAATGGCGTCATGGAAGTGGCTGGCCTTGCCGGGGACGACAAGGAGGCCGCGCGCGGCATCGTTCTGCGTG
CGATTGATAAGATGGACCGCTTGGGAGCGGGTGGGGTCCGTGATCTTCTGGGGGACGGCCGCAAGGATGAAAGCGGAGAT
TTCACCAAAGGAGCGGGGATATCTTCTGCCAATGCAGACAAGATACTTGCATTTGTACAAGCCACGGCTGGGGATAATGC
AGCGACGGTGTCCAACCTCAAGTCGCTGACGGAAGGGTCCGATATAGGCACGCAAGGCGTGTCGGAACTCGAACAAATCG
CCTCGCTGCTCGCCGCTCAGGGCTACGGCCCCGATCGTATCATCATCGACCCATCAGTCGTGCGCGGCCTCGGTTATTAC
ACCGGCCCCGTCTACGAGGCGGAGCTGACCTTCGACGTGCAGAACGAGAAGGGCCAGACCGTGCAGTTCGGATCTGTCGC
GGGCGGCGGGCGCTATGACGATCTCGTCAAGCGTTTCACCGGGCAAGAGGTTCCGGCCACGGGTGTCTCCATCGGGGTGG
ACCGGCTGCTCGCGGCATTGCACGCCACGGGCAAACTCAAAGGCGAAGATGCGGGCCCGGTGATCGTGACGGTCATGGAT
CGTGACCGGATGGCCGAGTATCAGGCGATGGTCGCGACCCTGCGCAATGCGGGCATTCGGGCGGAGGTGTATCTGGGCAA
CCCCAAGAACTTCGGCAACCAGTTGAAATACGCGGATAAGCGGCGGTCTCCTGTCGCGATTATCCAGGGCTCGGATGAGG
CCGCACGCGGCGTGGTGCAGCTGAAAGACCTGATCCTGGGCGCGAAACTGGCCGAGGATGCGACCTTGGAAGAGTGGAAA
TCCCAGCCCGCCCAGACAGAAGTCGCCGTCGCGGATCTGGTCGCTGAGGTCCAAGCCATCATCGCACGTCACAAGTGA

Upstream 100 bases:

>100_bases
TCTTCCAATTGCGTGATCTCAGACATCTCCATCGCCCTCCTTGCCCCTGTACGCCCCACCCGTTAGACGGTGCGCGATCC
GATTTGAAGGCACGCCCGCC

Downstream 100 bases:

>100_bases
CCCCCAAAGCTGCCATACGGGCCGAGGCGGAGGCGCTTCAGGCCACCTTCGCCGACGCCGTCCCCGTCGACGCCCCCATG
TTGGTCAGCGCGGAAACGCT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MAKQKKQPRPKAETPKGFRDYFGAEVVERQTMLRKIGEVYHRYGFDPLETSAVETVEALGKFLPDVDRPNEGVFAFQEDG
GSDDGKWLALRYDMTAPLARVAAQFRERPLPGQEDSLPSPYRRYTMGPVWRNEKPGPGRFRQFYQCDADTVGAPSVAADA
EICAMLADCLEAVGIERGDYVVRVNNRKVLNGVMEVAGLAGDDKEAARGIVLRAIDKMDRLGAGGVRDLLGDGRKDESGD
FTKGAGISSANADKILAFVQATAGDNAATVSNLKSLTEGSDIGTQGVSELEQIASLLAAQGYGPDRIIIDPSVVRGLGYY
TGPVYEAELTFDVQNEKGQTVQFGSVAGGGRYDDLVKRFTGQEVPATGVSIGVDRLLAALHATGKLKGEDAGPVIVTVMD
RDRMAEYQAMVATLRNAGIRAEVYLGNPKNFGNQLKYADKRRSPVAIIQGSDEAARGVVQLKDLILGAKLAEDATLEEWK
SQPAQTEVAVADLVAEVQAIIARHK

Sequences:

>Translated_505_residues
MAKQKKQPRPKAETPKGFRDYFGAEVVERQTMLRKIGEVYHRYGFDPLETSAVETVEALGKFLPDVDRPNEGVFAFQEDG
GSDDGKWLALRYDMTAPLARVAAQFRERPLPGQEDSLPSPYRRYTMGPVWRNEKPGPGRFRQFYQCDADTVGAPSVAADA
EICAMLADCLEAVGIERGDYVVRVNNRKVLNGVMEVAGLAGDDKEAARGIVLRAIDKMDRLGAGGVRDLLGDGRKDESGD
FTKGAGISSANADKILAFVQATAGDNAATVSNLKSLTEGSDIGTQGVSELEQIASLLAAQGYGPDRIIIDPSVVRGLGYY
TGPVYEAELTFDVQNEKGQTVQFGSVAGGGRYDDLVKRFTGQEVPATGVSIGVDRLLAALHATGKLKGEDAGPVIVTVMD
RDRMAEYQAMVATLRNAGIRAEVYLGNPKNFGNQLKYADKRRSPVAIIQGSDEAARGVVQLKDLILGAKLAEDATLEEWK
SQPAQTEVAVADLVAEVQAIIARHK
>Mature_504_residues
AKQKKQPRPKAETPKGFRDYFGAEVVERQTMLRKIGEVYHRYGFDPLETSAVETVEALGKFLPDVDRPNEGVFAFQEDGG
SDDGKWLALRYDMTAPLARVAAQFRERPLPGQEDSLPSPYRRYTMGPVWRNEKPGPGRFRQFYQCDADTVGAPSVAADAE
ICAMLADCLEAVGIERGDYVVRVNNRKVLNGVMEVAGLAGDDKEAARGIVLRAIDKMDRLGAGGVRDLLGDGRKDESGDF
TKGAGISSANADKILAFVQATAGDNAATVSNLKSLTEGSDIGTQGVSELEQIASLLAAQGYGPDRIIIDPSVVRGLGYYT
GPVYEAELTFDVQNEKGQTVQFGSVAGGGRYDDLVKRFTGQEVPATGVSIGVDRLLAALHATGKLKGEDAGPVIVTVMDR
DRMAEYQAMVATLRNAGIRAEVYLGNPKNFGNQLKYADKRRSPVAIIQGSDEAARGVVQLKDLILGAKLAEDATLEEWKS
QPAQTEVAVADLVAEVQAIIARHK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=473, Percent_Identity=29.1754756871036, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI6996014, Length=468, Percent_Identity=29.9145299145299, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1788861, Length=469, Percent_Identity=25.1599147121535, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71993686, Length=478, Percent_Identity=30.1255230125523, Blast_Score=185, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI71993693, Length=475, Percent_Identity=30.3157894736842, Blast_Score=184, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6325290, Length=509, Percent_Identity=27.7013752455796, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24643061, Length=516, Percent_Identity=30.8139534883721, Blast_Score=191, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24643059, Length=535, Percent_Identity=29.7196261682243, Blast_Score=182, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_JANSC (Q28TV0)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508887.1
- STRING:   Q28TV0
- GeneID:   3933389
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0945
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   IGKVFRD
- PhylomeDB:   Q28TV0
- ProtClustDB:   PRK00037
- BioCyc:   JSP290400:JANN_0945-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 54487; Mature: 54356

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKKQPRPKAETPKGFRDYFGAEVVERQTMLRKIGEVYHRYGFDPLETSAVETVEALG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KFLPDVDRPNEGVFAFQEDGGSDDGKWLALRYDMTAPLARVAAQFRERPLPGQEDSLPSP
HHCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCC
YRRYTMGPVWRNEKPGPGRFRQFYQCDADTVGAPSVAADAEICAMLADCLEAVGIERGDY
HHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
VVRVNNRKVLNGVMEVAGLAGDDKEAARGIVLRAIDKMDRLGAGGVRDLLGDGRKDESGD
EEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCC
FTKGAGISSANADKILAFVQATAGDNAATVSNLKSLTEGSDIGTQGVSELEQIASLLAAQ
CCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
GYGPDRIIIDPSVVRGLGYYTGPVYEAELTFDVQNEKGQTVQFGSVAGGGRYDDLVKRFT
CCCCCEEEECHHHHHHCCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHC
GQEVPATGVSIGVDRLLAALHATGKLKGEDAGPVIVTVMDRDRMAEYQAMVATLRNAGIR
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCE
AEVYLGNPKNFGNQLKYADKRRSPVAIIQGSDEAARGVVQLKDLILGAKLAEDATLEEWK
EEEEECCCCCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SQPAQTEVAVADLVAEVQAIIARHK
CCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKQKKQPRPKAETPKGFRDYFGAEVVERQTMLRKIGEVYHRYGFDPLETSAVETVEALG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KFLPDVDRPNEGVFAFQEDGGSDDGKWLALRYDMTAPLARVAAQFRERPLPGQEDSLPSP
HHCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCC
YRRYTMGPVWRNEKPGPGRFRQFYQCDADTVGAPSVAADAEICAMLADCLEAVGIERGDY
HHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
VVRVNNRKVLNGVMEVAGLAGDDKEAARGIVLRAIDKMDRLGAGGVRDLLGDGRKDESGD
EEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCC
FTKGAGISSANADKILAFVQATAGDNAATVSNLKSLTEGSDIGTQGVSELEQIASLLAAQ
CCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
GYGPDRIIIDPSVVRGLGYYTGPVYEAELTFDVQNEKGQTVQFGSVAGGGRYDDLVKRFT
CCCCCEEEECHHHHHHCCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHC
GQEVPATGVSIGVDRLLAALHATGKLKGEDAGPVIVTVMDRDRMAEYQAMVATLRNAGIR
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCE
AEVYLGNPKNFGNQLKYADKRRSPVAIIQGSDEAARGVVQLKDLILGAKLAEDATLEEWK
EEEEECCCCCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SQPAQTEVAVADLVAEVQAIIARHK
CCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA